scholarly journals Aquaporin 1, 3, and 5 Patterns in Salivary Gland Mucoepidermoid Carcinoma: Expression in Surgical Specimens and an In Vitro Pilot Study

2020 ◽  
Vol 21 (4) ◽  
pp. 1287 ◽  
Author(s):  
Mérin Barbara Stamboni ◽  
Ágatha Nagli de Mello Gomes ◽  
Milena Monteiro de Souza ◽  
Katia Klug Oliveira ◽  
Claudia Fabiana Joca Arruda ◽  
...  
Keyword(s):  
Salivary Gland ◽  
Mucoepidermoid Carcinoma ◽  
Tumor Biology ◽  
Tumor Stroma ◽  
Cell Phenotype ◽  
Mucosal Cells ◽  
Aqp1 Expression ◽  
Salivary Gland Malignancy ◽  
Vitro Model

Salivary gland aquaporins (AQPs) are essential for the control of saliva production and maintenance of glandular structure. However, little is known of their role in salivary gland neoplasia. Salivary gland tumors comprise a heterogeneous group of lesions, featuring variable histological characteristics and diverse clinical behaviors. Mucoepidermoid carcinoma (MEC) is the most common salivary gland malignancy. The aim of this study was to evaluate the expression of AQP1, AQP3, and AQP5 in 24 MEC samples by immunohistochemistry. AQP1 expression was observed in vascular endothelium throughout the tumor stroma. AQP3 was expressed in epidermoid and mucosal cells and AQP5 was expressed in mucosal cells of MEC. These proteins were expressed in the human MEC cell line UH-HMC-3A. Cellular ultrastructural aspects were analyzed by electron microscopy to certificate the tumor cell phenotype. In summary, our results show that, despite the fact that these molecules are important for salivary gland physiology, they may not play a distinct role in tumorigenesis in MEC. Additionally, the in vitro model may offer new possibilities to further investigate mechanisms of these molecules in tumor biology and their real significance in prognosis and possible target therapies.

An in vitro model for genetic changes associated with the adenomacarcinoma sequence in salivary gland tumors

1992 ◽  
Vol 63 (2) ◽  
pp. 118
Author(s):  
Bernd Kazmierczak ◽  
Brita Thode ◽  
Sabine Bartnitzke ◽  
Jörn Bullerdiek ◽  
Werner Schloot
Keyword(s):  
Salivary Gland ◽  
In Vitro Model ◽  
Salivary Gland Tumors ◽  
Genetic Changes ◽  
Vitro Model

scholarly journals Mucoepidermoid Carcinoma of the Palatine Tonsil

2015 ◽  
Vol 2015 ◽  
pp. 1-6
Author(s):  
Lucas Novaes Teixeira ◽  
Victor Angelo Martins Montalli ◽  
Luiz Carlos Santana Teixeira ◽  
Fabrício Passador-Santos ◽  
Andresa Borges Soares ◽  
...  
Keyword(s):  
Salivary Gland ◽  
Oral Cavity ◽  
Mucoepidermoid Carcinoma ◽  
Unusual Case ◽  
Cell Types ◽  
Palatine Tonsil ◽  
Soft Mass ◽  
Salivary Gland Malignancy ◽  
Adults And Children ◽  
Intermediate Cells

Mucoepidermoid carcinoma (MEC) is the most common primary salivary gland malignancy in both adults and children. It has a slight female predilection and usually presents as a painless, rubber-like or soft mass, which may be fixed or mobile. Histologically, MEC is comprised of a mixture of cell types including mucous, epidermoid, and intermediate cells that can be arranged in solid nests or cystic structures. In the oral cavity, it most frequently occurs at the palate or buccal mucosa. The present paper aimed to describe an unusual case of MEC arising in the palatine tonsil.

Silencing of CXCR4 inhibits the proliferation, adhesion, chemotaxis and invasion of salivary gland mucoepidermoid carcinoma Mc3 cells in vitro

Oral Oncology ◽  
2008 ◽  
Vol 44 (6) ◽  
pp. 545-554 ◽  
Author(s):  
De-Sheng Wen ◽  
Xiu-Li Zhu ◽  
Su-Min Guan ◽  
Yuan-Ming Wu ◽  
Li-Li Yu ◽  
...  
Keyword(s):  
Salivary Gland ◽  
Mucoepidermoid Carcinoma

scholarly journals Encapsulated multicellular tumor spheroids as a novel in vitro model to study small size tumors

2003 ◽  
Vol 57 (12) ◽  
pp. 585-588 ◽  
Author(s):  
Elena Markvicheva ◽  
Lina Bezdetnaya ◽  
Artur Bartkowiak ◽  
Annie Marc ◽  
Jean-Louis Gorgen ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Tumor Biology ◽  
Cell Encapsulation ◽  
Large Cell ◽  
In Vitro Models ◽  
Multicellular Tumor Spheroids ◽  
Tumor Spheroids ◽  
Vitro Model

Presently multicellular tumor spheroids (MTS) are being widely used in various aspects of tumor biology, including studies in biology and photodynamic therapy. The cellular organization of spheroids allows the recreation of in vivo small tumors much better than all common two-dimensional in vitro models. The cell encapsulation method could be proposed as a novel technique to quickly and easily prepare a large number of spheroids with narrow size distribution within a desirable diameter range. Moreover, the proposed technique for spheroid generation using encapsulated growing tumor cells could provide entirely new avenues to develop a novel spheroid co-culture model (for instance, the in vitro co-cultvation of tumor cells and monocytes, or epithelial cells, or fibroblasts etc). The current research was aimed at developing a simple and reliable method to encapsulate tumor cells and to cultivate them in vitro. In order to generate spheroids, MCF-7 cells were encapsulated and cultivated in 200 ml T-flasks in a 5% CO2 atmosphere at 37?C for 4-5 weeks. The cell proliferation was easily observed using a light microscope. The cells grew in aggregates increasing in size with time. The cell growth resulted in the formation of large cell clusters (spheroids) which filled the whole microcapsule volume in 4-5 weeks.

G1 Arrest and Caspase-Mediated Apoptosis in HL-60 Cells by Dichloromethane Extract of Centrosema pubescens

2010 ◽  
Vol 38 (06) ◽  
pp. 1143-1159 ◽  
Author(s):  
Subramanireddy Ramadevi Mani ◽  
Baddireddi Subhadra Lakshmi
Keyword(s):  
Cell Cycle ◽  
Mononuclear Cells ◽  
Tumor Biology ◽  
G1 Phase ◽  
G1 Arrest ◽  
Synergistic Activity ◽  
Peripheral Blood Mononuclear ◽  
Normal Peripheral Blood ◽  
Vitro Model

Cell division and apoptosis are two crucial components of tumor biology and the importance of increased cell proliferation and reduced cell death have made them valid therapeutic targets. The plant kingdom is a relatively underexploited cache of novel drugs, and crude extracts of plants are known for their synergistic activity. The present study assessed the anti-proliferative activity of the medicinal plant Centrosema pubescens Benth. Centrosema pubescens dichloromethane extract (CPDE) inhibited the proliferation of HL-60 (promyelocytic acute leukaemia) cells with an IC 50 value of 5 μg/ml. Further studies also showed that CPDE induces growth arrest at the G1 phase and specifically down-regulates the expressions of cyclin E and CDK2 and up-regulates p27(CKI) levels. These events apparently lead to the induction of apoptosis, which was demonstrated qualitatively by a DNA fragmentation assay and propidium iodide staining. Quantitative assessment of the effective arrest of the cell cycle and of apoptosis was confirmed by flow cytometry. CPDE exhibited negligible cytotoxicity even at the highest dose tested (100 μg/ml) in both normal peripheral blood mononuclear cells and in an in vitro model (HL-60). Our results strongly suggest that CPDE arrests the cell cycle at the G1 phase and triggers apoptosis by caspase activation.

scholarly journals Isolation and characterization of biliary epithelial and stromal cells from resected human cholangiocarcinoma: A novel in vitro model to study tumor-stroma interactions

2013 ◽  
Vol 30 (3) ◽  
pp. 1143-1148 ◽  
Author(s):  
MARCO MASSANI ◽  
TOMMASO STECCA ◽  
LUCA FABRIS ◽  
EZIO CARATOZZOLO ◽  
CESARE RUFFOLO ◽  
...  
Keyword(s):  
Stromal Cells ◽  
In Vitro Model ◽  
Tumor Stroma ◽  
Isolation And Characterization ◽  
Vitro Model

Isolated mucoepidermoid carcinoma in the neck

1998 ◽  
Vol 119 (4) ◽  
pp. 398-399 ◽  
Author(s):  
Manish K. Wani ◽  
K. Thomas Robbins ◽  
Frank S. H. Wong ◽  
Todd E. Stiles
Keyword(s):  
Salivary Gland ◽  
Lymph Node ◽  
Lymph Nodes ◽  
Mucoepidermoid Carcinoma ◽  
Cervical Lymph Node ◽  
Common Type ◽  
Primary Site ◽  
Cervical Lymph Nodes ◽  
Salivary Gland Malignancy

Mucoepidermoid carcinoma is a common type of salivary gland malignancy with metastasis to the cervical lymph nodes occurring in up to 29% of patients. 1 We describe three patients presenting with isolated mucoepidermoid carcinoma in a cervical lymph node without an obvious primary site of origin.

scholarly journals Survival of salivary gland cancer stem cells requires mTOR signaling

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Nathalia P. Andrade ◽  
Kristy A. Warner ◽  
Zhaocheng Zhang ◽  
Alexander T. Pearson ◽  
Andrea Mantesso ◽  
...  
Keyword(s):  
Salivary Gland ◽  
Tumor Cells ◽  
Mucoepidermoid Carcinoma ◽  
Therapeutic Potential ◽  
Mtor Pathway ◽  
Mtor Signaling ◽  
Salivary Gland Cancer ◽  
Self Renewal ◽  
Immunodeficient Mice

AbstractAdvanced salivary gland mucoepidermoid carcinoma (MEC) is a relentless cancer that exhibits resistance to conventional chemotherapy. As such, treatment for patients with advanced MEC is tipically radical surgery and radiotherapy. Facial disfigurement and poor quality of life are frequent treatment challenges, and many patients succumb to loco-regional recurrence and/or metastasis. We know that cancer stem-like cells (CSC) drive MEC tumorigenesis. The current study tests the hypothesis that MEC CSC are sensitive to therapeutic inhibition of mTOR. Here, we report a correlation between the long-term clinical outcomes of 17 MEC patients and the intratumoral expression of p-mTOR (p = 0.00294) and p-S6K1 (p = 0.00357). In vitro, we observed that MEC CSC exhibit constitutive activation of the mTOR signaling pathway (i.e., mTOR, AKT, and S6K1), unveiling a potential strategy for targeted ablation of these cells. Using a panel of inhibitors of the mTOR pathway, i.e., rapamycin and temsirolimus (mTOR inhibitors), buparlisib and LY294002 (AKT inhibitors), and PF4708671 (S6K1 inhibitor), we observed consistently dose-dependent decrease in the fraction of CSC, as well as inhibition of secondary sphere formation and self-renewal in three human MEC cell lines (UM-HMC-1,-3A,-3B). Notably, therapeutic inhibition of mTOR with rapamycin or temsirolimus induced preferential apoptosis of CSC, when compared to bulk tumor cells. In contrast, conventional chemotherapeutic drugs (cisplatin, paclitaxel) induced preferential apoptosis of bulk tumor cells and accumulation of CSC. In vivo, therapeutic inhibition of mTOR with temsirolimus caused ablation of CSC and downregulation of Bmi-1 expression (major inducer of stem cell self-renewal) in MEC xenografts. Transplantation of MEC cells genetically silenced for mTOR into immunodeficient mice corroborated the results obtained with temsirolimus. Collectively, these data demonstrated that mTOR signaling is required for CSC survival, and unveiled the therapeutic potential of targeting the mTOR pathway for elimination of highly tumorigenic cancer stem-like cells in salivary gland mucoepidermoid carcinoma.

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