scholarly journals L1CAM as an E-selectin Ligand in Colon Cancer

2020 ◽  
Vol 21 (21) ◽  
pp. 8286
Author(s):  
Fanny M. Deschepper ◽  
Roberta Zoppi ◽  
Martina Pirro ◽  
Paul J. Hensbergen ◽  
Fabio Dall’Olio ◽  
...  
Keyword(s):  
Cell Line ◽  
Sialyl Lewis X ◽  
The Novel ◽  
Migration Ability ◽  
Sialyl Lewis ◽  
Selectin Ligands ◽  
Neural Cell Adhesion ◽  
Selectin Ligand ◽  
Carbohydrate Ligands ◽  
Cell Adhesion Molecule L1

Metastasis is the main cause of death among colorectal cancer (CRC) patients. E-selectin and its carbohydrate ligands, including sialyl Lewis X (sLeX) antigen, are key players in the binding of circulating tumor cells to the endothelium, which is one of the major events leading to organ invasion. Nevertheless, the identity of the glycoprotein scaffolds presenting these glycans in CRC remains unclear. In this study, we firstly have characterized the glycoengineered cell line SW620 transfected with the fucosyltransferase 6 (FUT6) coding for the α1,3-fucosyltransferase 6 (FUT6), which is the main enzyme responsible for the synthesis of sLeX in CRC. The SW620FUT6 cell line expressed high levels of sLeX antigen and E-selectin ligands. Moreover, it displayed increased migration ability. E-selectin ligand glycoproteins were isolated from the SW620FUT6 cell line, identified by mass spectrometry, and validated by flow cytometry and Western blot (WB). The most prominent E-selectin ligand we identified was the neural cell adhesion molecule L1 (L1CAM). Previous studies have shown association of L1CAM with metastasis in cancer, thus the novel role as E-selectin counter-receptor contributes to understand the molecular mechanism involving L1CAM in metastasis formation.

Sulfated Sialyl Lewis X, the Putative L-Selectin Ligand, Detected on Endothelial Cells of High Endothelial Venules by a Distinct Set of Anti-Sialyl Lewis X Antibodies

1997 ◽  
Vol 230 (3) ◽  
pp. 546-551 ◽  
Author(s):  
Chikako Mitsuoka ◽  
Naoko Kawakami-Kimura ◽  
Mikiko Kasugai-Sawada ◽  
Nozomu Hiraiwa ◽  
Ken'ichi Toda ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Sialyl Lewis X ◽  
Lewis X ◽  
High Endothelial Venules ◽  
Sialyl Lewis ◽  
Selectin Ligand

scholarly journals Selectin Ligands Sialyl-Lewis a and Sialyl-Lewis x in Gastrointestinal Cancers

Biology ◽  
2017 ◽  
Vol 6 (4) ◽  
pp. 16 ◽  
Author(s):  
Marco Trinchera ◽  
Adele Aronica ◽  
Fabio Dall’Olio
Keyword(s):  
Sialyl Lewis X ◽  
Gastrointestinal Cancers ◽  
Lewis X ◽  
Sialyl Lewis ◽  
Selectin Ligands ◽  
Sialyl Lewis A ◽  
Lewis A

scholarly journals Identification of a Major Carbohydrate Capping Group of the L-selectin Ligand on High Endothelial Venules in Human Lymph Nodes as 6-Sulfo Sialyl Lewis X

1998 ◽  
Vol 273 (18) ◽  
pp. 11225-11233 ◽  
Author(s):  
Chikako Mitsuoka ◽  
Mikiko Sawada-Kasugai ◽  
Keiko Ando-Furui ◽  
Mineko Izawa ◽  
Hayao Nakanishi ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
Sialyl Lewis X ◽  
Lewis X ◽  
High Endothelial Venules ◽  
Sialyl Lewis ◽  
Selectin Ligand

Biosynthesis of L-Selectin Ligands:  Sulfation of Sialyl Lewis x-Related Oligosaccharides by a Family of GlcNAc-6-sulfotransferases†

Biochemistry ◽  
2001 ◽  
Vol 40 (18) ◽  
pp. 5382-5391 ◽  
Author(s):  
Kendra G. Bowman ◽  
Brian N. Cook ◽  
Christopher L. de Graffenried ◽  
Carolyn R. Bertozzi
Keyword(s):  
Sialyl Lewis X ◽  
Lewis X ◽  
Sialyl Lewis ◽  
Selectin Ligands

O-glycans on human high endothelial CD34 putatively participating in L-selectin recognition

Blood ◽  
2002 ◽  
Vol 99 (7) ◽  
pp. 2609-2611 ◽  
Author(s):  
Tero Satomaa ◽  
Ossi Renkonen ◽  
Jari Helin ◽  
Juha Kirveskari ◽  
Antti Mäkitie ◽  
...  
Keyword(s):  
Laser Desorption ◽  
Sialyl Lewis X ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Flight Mass Spectrometry ◽  
Sialyl Lewis ◽  
Candidate Structure ◽  
Selectin Ligands ◽  
Matrix Assisted Laser

Leukocyte traffic into lymph nodes and sites of inflammation is guided by L-selectin. Experiments performed in vitro and with gene-deleted mice suggest that CD34 recognizes L-selectin if decorated by 6-sulfo sialyl Lewis x (sLex) saccharides and the MECA-79 epitope. However, very little is known about glycosylation of human L-selectin ligands. We report here on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) profiles of N- and O-linked oligosaccharide fractions from human tonsillar endothelial CD34. All detected O-glycans were sialylated; some were also monosulfated or monosulfated and monofucosylated. If a given CD34-glycan may carry all requirements for L-selectin recognition, that is, both 6-sulfo-sLex and MECA-79 epitopes, only one O-glycan fraction, O-9, SA2Hex3HexNAc3- Fuc1(SO3)1, meets the criteria. A candidate structure is SAα2-3Galβ1-4(Fucα1-3)(6-sulfo)GlcNAcβ1-3Galβ1-3(SAα2-3Galβ1-4GlcNAcβ1-6)GalNAc. However, if sulfo sLex glycans are supplemented with separate sulfated, nonfucosylated O-glycans, saccharides in O-6, O-8, or O-9, putatively carrying MECA-79 epitopes, could form multiglycan binding epitopes for L-selectin.

scholarly journals Human Synovial Lubricin Expresses Sialyl Lewis x Determinant and Has L-selectin Ligand Activity

2012 ◽  
Vol 287 (43) ◽  
pp. 35922-35933 ◽  
Author(s):  
Chunsheng Jin ◽  
Anna-Karin Hultgård Ekwall ◽  
Johan Bylund ◽  
Lena Björkman ◽  
Ruby P. Estrella ◽  
...  
Keyword(s):  
Sialyl Lewis X ◽  
Lewis X ◽  
Sialyl Lewis ◽  
Selectin Ligand ◽  
Ligand Activity
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