scholarly journals Evaluation of Reference Genes and Expression Level of Genes Potentially Involved in the Mode of Action of Cry1Ac and Cry1F in a Susceptible Reference Strain of Chrysodeixis includens

Insects ◽  
2021 ◽  
Vol 12 (7) ◽  
pp. 598
Author(s):  
Macarena Martin ◽  
Debora Boaventura ◽  
Ralf Nauen
Keyword(s):  
Genetically Modified Crops ◽  
Control Measures ◽  
Larval Stages ◽  
Basal Expression ◽  
Protein Receptor ◽  
Bt Protein ◽  
High Selection ◽  
Gene Study ◽  
Soybean Looper ◽  
Chrysodeixis Includens

Soybean looper (SBL), Chrysodeixis includens (Walker), is one of the major lepidopteran pests of soybean in the American continent. SBL control relies mostly on the use of insecticides and genetically modified crops expressing Bacillus thuringiensis (Bt) insecticidal Cry proteins. Due to the high selection pressure exerted by these control measures, resistance has developed to different insecticides and Bt proteins. Nevertheless, studies on the mechanistic background are still scarce. Here, the susceptibility of the laboratory SBL-Benzon strain to the Bt proteins Cry1Ac and Cry1F was determined in diet overlay assays and revealed a greater activity of Cry1Ac than Cry1F, thus confirming results obtained for other sensitive SBL strains. A reference gene study across larval stages with four candidate genes revealed that RPL10 and EF1 were the most stable genes for normalization of gene expression data obtained by RT-qPCR. Finally, the basal expression levels of eight potential Bt protein receptor genes in six larval instars were analyzed, including ATP-binding cassette (ABC) transporters, alkaline phosphatase, aminopeptidases, and cadherin. The results presented here provide fundamental knowledge to support future SBL resistance studies.

Field-evolved resistance to chitin synthesis inhibitor insecticides by soybean looper, Chrysodeixis includens (Lepidoptera: Noctuidae), in Brazil

Chemosphere ◽  
2020 ◽  
Vol 259 ◽  
pp. 127499
Author(s):  
Regis F. Stacke ◽  
Daniela N. Godoy ◽  
Venicius E. Pretto ◽  
Fábio M. Führ ◽  
Patricia da S. Gubiani ◽  
...  
Keyword(s):  
Chitin Synthesis ◽  
Synthesis Inhibitor ◽  
Chitin Synthesis Inhibitor ◽  
Soybean Looper ◽  
Lepidoptera Noctuidae ◽  
Chrysodeixis Includens

scholarly journals Efficacy of Chrysodeixis Includens Nucleopolyhedrovirus for Control of Soybean Looper, 2016

2020 ◽  
Vol 45 (1) ◽  
Author(s):  
Joe Black ◽  
Gus Lorenz ◽  
Nicki Taillon ◽  
Andrew Plummer ◽  
Aaron Cato ◽  
...  
Keyword(s):  
Soybean Looper ◽  
Chrysodeixis Includens

scholarly journals Complete Genome Sequences of Seven New Chrysodeixis includens Nucleopolyhedrovirus Isolates from Minas Gerais and Mato Grosso States in Brazil

2020 ◽  
Vol 9 (8) ◽  
Author(s):  
Saluana R. Craveiro ◽  
Peter W. Inglis ◽  
Laura Lisieux S. Monteiro ◽  
Luis Arthur V. M. Santos ◽  
Roberto C. Togawa ◽  
...  
Keyword(s):  
Open Reading Frames ◽  
Southeastern Brazil ◽  
Genome Sequences ◽  
Mato Grosso ◽  
Content Type ◽  
Soybean Looper ◽  
Reading Frames ◽  
Viral Isolates ◽  
Chrysodeixis Includens ◽  
Complete Genomic

We report the complete genomic sequences of seven viral isolates from the soybean looper (Chrysodeixis includens) from midwestern and southeastern Brazil. The genomes range from 138,760 to 139,637 bp in length with a G+C content of 39.2% and 140 open reading frames (ORFs).

scholarly journals Susceptibility of soybean looper to lufenuron and spinosad

2020 ◽  
Vol 87 ◽  
Author(s):  
Lucas Ferraz de Queiroz ◽  
Janaina De Nadai Corassa ◽  
Sandra Maria Morais Rodrigues ◽  
Rafael Major Pitta
Keyword(s):  
Chemical Control ◽  
Resistance Management ◽  
The State ◽  
Laboratory Population ◽  
Mato Grosso ◽  
Regional Monitoring ◽  
Pest Monitoring ◽  
Overlay Method ◽  
Soybean Looper ◽  
Chrysodeixis Includens

ABSTRACT: The soybean looper (Chrysodeixis includens) is an important defoliation pest in crops such as soybean and cotton in Brazil. Its main control tactic is chemical insecticides. Considering the importance of chemical control for this pest, monitoring the susceptibility of C. includens populations is strategic for an efficient Insect Resistance Management. Therefore, the objective of this study was to evaluate the susceptibility levels of C. includens populations in the state of Mato Grosso - Brazil to lufenuron and spinosad. Seven populations were collected in soybean fields around the state. For the bioassays, early L3 larvae were exposed to insecticides using the diet-overlay method. Although the compounds have distinct modes of action, Tangará da Serra population had the highest resistance ratios for lufenuron (11.62) and spinosad (7.84), compared to laboratory population (susceptibility reference). Even with low resistance levels, it is necessary to maintain regional monitoring of C. includens susceptibility to the evaluated insecticides, as well as to extend the range of molecules monitored.

The life-history and control of Hapatesus hirtus Candeze (Coleoptera: Elateridae) in Victoria

1991 ◽  
Vol 42 (5) ◽  
pp. 827 ◽  
Author(s):  
PA Horne ◽  
JA Horne
Keyword(s):  
Life History ◽  
Field Trials ◽  
Soil Samples ◽  
Control Measures ◽  
Potato Tubers ◽  
Larval Stages ◽  
Complete Development ◽  
History Of ◽  
And Control ◽  
Potato Crops

The incidence and life-history of potato wireworm, Hapatesus hirtus Candeze, in Victorian potato crops were studied, and insecticidal control measures assessed. Larval stages were present and active throughout the year. Ten larval instars were identified, probably requiring several years to complete development. Egg development occurred in October-November and the most likely timing of oviposition was December. The relative numbers of larval H. hirtus detected in soil samples, pre-planting baits and harvested tubers are presented. Baiting with halves of potatoes provided an efficient means of detecting areas likely to suffer wireworm damage. One formulation of chlorpyrifos ('Lorsban') was effective in reducing damage to, and larval numbers in, potatoes in field trials. The granular formulation of tefluthrin and the controlled release chlorpyrifos were not effective in protecting potato tubers.

Basal Expression of Bone Morphogenetic Protein Receptor Is Reduced in Mild Asthma

2008 ◽  
Vol 177 (10) ◽  
pp. 1074-1081 ◽  
Author(s):  
Harsha H. Kariyawasam ◽  
Georgina Xanthou ◽  
Julia Barkans ◽  
Maxine Aizen ◽  
A. Barry Kay ◽  
...  
Keyword(s):  
Bone Morphogenetic Protein ◽  
Mild Asthma ◽  
Bone Morphogenetic Protein Receptor ◽  
Basal Expression ◽  
Protein Receptor ◽  
Morphogenetic Protein

scholarly journals Antixenosis of bean genotypes to Chrysodeixis includens (Lepidoptera: Noctuidae)

2015 ◽  
Vol 50 (6) ◽  
pp. 450-458 ◽  
Author(s):  
Rafaela Morando ◽  
Edson Luiz Lopes Baldin ◽  
Patrícia Leite Cruz ◽  
André Luiz Lourenção ◽  
Alisson Fernando Chiorato
Keyword(s):  
Free Choice ◽  
Oviposition Preference ◽  
Green Light ◽  
Choice Test ◽  
Choice Tests ◽  
Dark Green ◽  
Soybean Looper ◽  
Lepidoptera Noctuidae ◽  
Light Green ◽  
Chrysodeixis Includens

The objective of this work was to evaluate bean genotypes for resistance to soybean looper (Chrysodeixis includens). Initially, free-choice tests were carried out with 59 genotypes, divided into three groups according to leaf color intensity (dark green, light green, and medium green), in order to evaluate oviposition preference. Subsequently, 12 genotypes with high potential for resistance were selected, as well as two susceptible commercial standards. With these genotypes, new tests were performed for oviposition in a greenhouse, besides tests for attractiveness and consumption under laboratory conditions (26±2ºC, 65±10% RH, and 14 h light: 10 h dark photophase). In the no-choice test with adults, in the greenhouse, the 'IAC Jabola', Arcelina 1, 'IAC Boreal', 'Flor de Mayo', and 'IAC Formoso' genotypes were the least oviposited, showing antixenosis-type resistance for oviposition. In the free-choice test with larvae, Arcelina 4, 'BRS Horizonte', 'Pérola', H96A102-1-1-1-52, 'IAC Boreal', 'IAC Harmonia', and 'IAC Formoso' were the less consumed genotypes, which indicates antixenosis to feeding. In the no-choice test, all genotypes (except for 'IAPAR 57') expressed moderate levels of antixenosis to feeding against C. includens larvae.

scholarly journals Population expansion and genomic adaptation to agricultural environments of the soybean looper, Chrysodeixis includens

2020 ◽  
Vol 13 (8) ◽  
pp. 2071-2085 ◽  
Author(s):  
Cleane S. Silva ◽  
Erick M.G. Cordeiro ◽  
Julia B. Paiva ◽  
Patrick M. Dourado ◽  
Renato A. Carvalho ◽  
...  
Keyword(s):  
Population Expansion ◽  
Genomic Adaptation ◽  
Soybean Looper ◽  
Chrysodeixis Includens
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