scholarly journals Higher Number of EBI3 Cells in Mucosal Chronic Hyperplastic Candidiasis May Serve to Regulate IL-17-Producing Cells

2021 ◽  
Vol 7 (7) ◽  
pp. 533
Author(s):  
Ailish Williams ◽  
Helen Rogers ◽  
David Williams ◽  
Xiao-Qing Wei ◽  
Damian Farnell ◽  
...  
Keyword(s):  
Oral Mucosa ◽  
Oral Lichen Planus ◽  
Inflammatory Cell ◽  
Candida Infection ◽  
Inflammatory Cell Infiltrate ◽  
T Helper ◽  
Cell Infiltrate ◽  
High Prevalence ◽  
Squamous Cell Papilloma ◽  
Oral Lichen

Previous research into the inflammatory cell infiltrate of chronic hyperplastic candidosis (CHC) determined that the immune response is primarily composed of T cells, the majority of which are T helper (CD4+) cells. This present investigation used immunohistochemistry to further delineate the inflammatory cell infiltrate in CHC. Cells profiled were those expressing IL-17A cytokine, EBI3 and IL-12A subunits of the IL-35 cytokine, and FoxP3+ cells. Squamous cell papilloma (with Candida infection) and oral lichen planus tissues served as comparative controls to understand the local immune responses to Candida infection. The results demonstrated that Candida-induced inflammation and immune regulation co-exist in the oral mucosa of CHC and that high prevalence of cells expressing the EBI3 cytokine subunit may play an important role in this regulation. This balance between inflammation and immune tolerance toward invading Candida in the oral mucosa may be critical in determining progress of infection.

scholarly journals Thickness of the epithelium and the inflammatory cell infiltrate in oral lichen planus. A morphometric study

2014 ◽  
Vol 6 (2) ◽  
pp. 78-82
Author(s):  
D.B Nandini ◽  
VV Ramya
Keyword(s):  
Image Analysis ◽  
Lichen Planus ◽  
Oral Lichen Planus ◽  
Inflammatory Cell ◽  
Morphometric Study ◽  
Inflammatory Cell Infiltrate ◽  
Analysis Software ◽  
Cell Infiltrate ◽  
Image Analysis Software ◽  
Oral Lichen

Abstract Background Oral lichen planus is a chronic mucocutaneous disease of uncertain etiology. Inflammatory cell infiltrate plays an important role in pathogenesis of oral lichen planus. It is said that hyperplastic epithelium is seen where the inflammatory cell infiltrate is mild while beneath atrophic epithelium dense inflammatory cell infiltrate is evident. Studies have shown negative correlation between thickness of the epithelium and thickness of inflammatory cell infiltrate. Morphometric studies in oral lichen planus are very scanty and have been performed using stage micrometer and eye piece graticule. Using Image Analysis Software can avoid inter-observer variations which has not been done till date. Aim and Objectives The present study aimed at evaluating thickness of epithelium and thickness of inflammatory cell infiltrate and to determine any existing relation between the same using Image Analysis Software. Other histopathologic features were also evaluated. Materials and method 58 confirmed cases of oral lichen planus from buccal mucosa were retrieved from department archives. 6μ thick sections were stained with Hematoxylin and Eosin. Six non-overlapping fields were selected randomly from each section and photomicrographs were captured under 4x objective using Trinocular Research Microscope. Morphometry was done using Image Analysis Software and data was stored in Microsoft excel for statistical analysis. Results In present study the mean of epithelium thickness was 281.2059 and inflammatory cell infiltrate thickness was 330.2540. An inverse correlation was observed. As the thickness of inflammatory cell infiltrate increased there was a decrease in thickness of epithelium (Coefficient -0.156). Conclusion Thickness of the epithelium may vary according to the site, however in this study all cases were from buccal mucosa. Inflammatory cell infiltrate influences overlying thickness of epithelium and determines its nature. How to cite this article Ramya VV, Nandini DB, Praveen SB, Madhushankari GS. Thickness of the epithelium and the inflammatory cell infiltrate in oral lichen planus. A morphometric study. CODS J Dent 2014;6;78-82

Characterisation of the inflammatory cell infiltrate in chronic hyperplastic candidosis of the oral mucosa

1997 ◽  
Vol 26 (2) ◽  
pp. 83-89 ◽  
Author(s):  
D. W. Williams ◽  
A. J. C. Potts ◽  
M. J. Wilson ◽  
J. B. Matthews ◽  
M. A. O. Lewis
Keyword(s):  
Oral Mucosa ◽  
Inflammatory Cell ◽  
Inflammatory Cell Infiltrate ◽  
Cell Infiltrate

Criteria for assessing severity of oral lichen planus

MedAlliance ◽  
2021 ◽  
Vol 9 (1) ◽  
pp. 73-81
Keyword(s):  
Lichen Planus ◽  
Oral Mucosa ◽  
Severity Score ◽  
Oral Lichen Planus ◽  
Unknown Etiology ◽  
Altered Level ◽  
Doppler Flowmetry ◽  
Serum Free ◽  
Control Subjects ◽  
Oral Lichen

Introduction. Oral lichen planus (OLP) is an autoim- mune disease of unknown etiology, manifested in vari- ous clinical forms. A correlation between intensity of OLP local manifestations and severity of general disturbances predisposing to altered oral mucosa have been poorly investigated. The aim of our study was to identify criteria for assessing OLP severity score. Methods. OLP patients and control subjects (not OLP) matched by hepatobiliary disease and type IIa dyslipidemia were examined. Samples collected during the study were as- sessed by way clinical examination, gas chromatographic analysis of serum free fatty acids (FFA), oral fluid, serum and fecal microbial marker analysis by gas chromatogra- phy/mass spectrometry, laser Doppler flowmetry of the oral mucosa, immunohistochemistry staining with an- ti-CD68 monoclonal antibody specific to apolipoprotein B-100-bearing macrophages. Results. It was found that OLP patients significantly differed from control subjects in the three FFA levels (lauric acid, eicosopentaenoic/ lauric and eicosopentaenoic/linoleic ratios), displayed four pathologic factors matching the altered level of mi- crobial markers. In addition, OLP patients were found to differ in oral mucosa blood filling both inside and outside lesions paralleled with oxidative changes immediately in oral mucosa highlighted as elevated amount of apo- lipoprotein B-100-bearing CD68-positive macrophages. Conclusion. OLP severity score is an integrate parameter which reflects oxidative changes and alterations in oral mucosa microhemocirculation primarily in serum free fat- ty acid composition, altered oral and gut microbiota as well as intensity score of clinical picture.

scholarly journals Evaluation of Cellular Proliferative Activity in Patients with Oral Lichen Planus and Hepatitis C through AgNOR Method

2014 ◽  
Vol 25 (6) ◽  
pp. 461-465
Author(s):  
João Paulo De Carli ◽  
Soluete Oliveira da Silva ◽  
Maria Salete Sandini Linden ◽  
Carmen Silvia Busin ◽  
Luiz Renato Paranhos ◽  
...  
Keyword(s):  
Hepatitis C ◽  
Lichen Planus ◽  
Oral Mucosa ◽  
Oral Lichen Planus ◽  
Clinical Signs ◽  
Hcv Infection ◽  
Control Group ◽  
Proliferative Potential ◽  
Group 2 ◽  
Oral Lichen

The objective of this study was to evaluate the cellular proliferative potential of oral lichen planus (OLP) lesions from patients without hepatitis C virus (HCV) by means of AgNOR method, as well as the cellular proliferative potential of the normal oral mucosa from patients with HCV, treated or untreated by interferon and ribavirin. A cross-sectional study was developed to investigate four groups: 10 HCV+ patients without clinical signs of OLP who had never been treated for HCV infection - Group 1; 10 HCV+ patients that were under interferon and ribavirin treatment - Group 2; 15 patients with reticular OLP lesions histopathologically confirmed, without HCV - Group 3; and 15 blood donors without HCV infection and no clinical signs of OLP GROUP 4 Control Group. The cytological material of all groups was collected by the liquid-based cytology technique. Then, the sedimented material from each patient was filled with the Nucleolar Organizer Regions impregnation by silver method (AgNOR). The count of NORs was performed on 100 epithelial cell nuclei per patient using the Image Tool(tm) software. The Tukey HSD test was used to compare the median value of NORs among the groups and showed that the oral mucosa of HCV+ patients previously treated with anti-HCV drugs (GROUP 2), presented a higher average number of NORs in relation to others (p<0.05). The anti-HCV treatment may be related to increased cell proliferation of oral mucosa, indicating a possible relationship between OLP and HCV+ patients treated with interferon and ribavirin.

scholarly journals Role of apoptosis in erosive and reticular oral lichen planus exhibiting variable epithelial thickness

2008 ◽  
Vol 19 (3) ◽  
pp. 179-185 ◽  
Author(s):  
Juliana M. Caldeira Brant ◽  
Anilton C. Vasconcelos ◽  
Luciana V. Rodrigues
Keyword(s):  
Lichen Planus ◽  
Oral Mucosa ◽  
Oral Lichen Planus ◽  
Chronic Inflammatory Disease ◽  
Mechanical Trauma ◽  
Epithelial Thickness ◽  
Healthy Mucosa ◽  
Toxic Agents ◽  
Oral Lichen

Oral lichen planus (OLP) is a chronic inflammatory disease with different clinical types. Reticular and erosive forms are the most common. Although the cause of OLP remains speculative, many findings suggest auto-immune involvement, mediated by T lymphocytes against the basal keratinocytes. Inflammation, mechanical trauma or toxic agents can affect the epithelial homeostasia. Increased apoptosis may cause a decrease in epithelial thickness reflecting in the activity of the lesion. The objective of this study was to evaluate the occurrence of apoptosis and epithelial thickness in reticular and erosive forms of OLP. 15 samples of OLP each type (reticular and erosive) plus 10 of healthy mucosa were collected and processed. After morphometry, the apoptotic index and epitelial thickness were obtained. TUNEL and M30 CytoDEATH immunohistochemical assay were used to validate the morphologic criteria used. Apoptosis in the erosive OLP was significantly more intense than in the reticular type and both forms of OLP presented more apoptosis than the healthy oral mucosa. Healthy oral mucosa was thicker than both OLP forms and thicker in OLP reticular form than in the erosive one. The clinical differences between reticular and erosive forms of OLP are related to variations in epithelial thickness and in intensity of apoptosis.

scholarly journals Familial oral lichen planus in a 3-year-old boy: a case report with eight years of follow-up

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Fang Wang ◽  
Ya-Qin Tan ◽  
Jing Zhang ◽  
Gang Zhou
Keyword(s):  
Lichen Planus ◽  
Oral Mucosa ◽  
Buccal Mucosa ◽  
Oral Lichen Planus ◽  
Familial Aggregation ◽  
Pediatric Population ◽  
Case Presentation ◽  
Oral Lichen

Abstract Background Oral lichen planus (OLP) is a chronic mucocutaneous disease characterized by adult predominance and a prolonged course. However, it is rare in the pediatric population with familial aggregation. Case presentation A 3-year-old boy presented with pain and irritation on the oral mucosa while contacting spicy food for 2 months. Oral examination showed widespread whitish reticular and papular lesions on the lips, the dorsum of the tongue, and bilateral buccal mucosa, with diffuse erosions covered with pseudomembrane on the buccal mucosa. The boy’s parents were examined to exhibit white reticular and plaque-like lesions on their oral mucosa. The three patients were clinically diagnosed as affected by OLP and histopathologically confirmed. The boy underwent topical treatment with recombinant bovine basic fibroblast growth factor (rb-bFGF) gel, and oral lesions gradually resolved and healed. Neither of his parents received treatment. During the subsequent follow-ups, none of three patients underwent any medical treatment. Fortunately, their lesions had almost faded over 8 years. Conclusions Our case emphasizes that pediatric OLP should be recorded with family history. Besides, long-term periodic follow-up is recommended in pediatric patients with OLP for monitoring any changes in lesions.

scholarly journals DNA Vaccines: MHC II-Targeted Vaccine Protein Produced by Transfected Muscle Fibres Induces a Local Inflammatory Cell Infiltrate in Mice

PLoS ONE ◽  
2014 ◽  
Vol 9 (10) ◽  
pp. e108069 ◽  
Author(s):  
Tom-Ole Løvås ◽  
Jo C. Bruusgaard ◽  
Inger Øynebråten ◽  
Kristian Gundersen ◽  
Bjarne Bogen
Keyword(s):  
Inflammatory Cell ◽  
Dna Vaccines ◽  
Inflammatory Cell Infiltrate ◽  
Muscle Fibres ◽  
Cell Infiltrate ◽  
Mhc Ii
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