scholarly journals Correlations between Electrophysiological Parameters, Lymphocyte Distribution and Cytokine Levels in Patients with Chronic Demyelinating Inflammatory Polyneuropathy

2021 ◽  
Vol 11 (8) ◽  
pp. 766
Author(s):  
Edyta Dziadkowiak ◽  
Helena Moreira ◽  
Malgorzata Wieczorek ◽  
Slawomir Budrewicz ◽  
Ewa Barg ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Compound Muscle Action Potential ◽  
Control Group ◽  
Autoimmune Process ◽  
Inflammatory Infiltration ◽  
Muscle Action Potential ◽  
Tnf Α ◽  
Cytokine Levels ◽  
Immunoglobulin Treatment ◽  
Inflammatory Polyneuropathy

The goal of this study was to analyse, in relation to electrophysiological results, the distribution of lymphocyte subpopulations and the level of cytokines in patients with the typical form of chronic demyelinating inflammatory polyneuropathy (CIDP) before immunoglobulin treatment. The study group consisted of 60 patients (52 men, eight women), with a mean age 64.8 ± 11.2, who fulfilled the diagnostic criteria for the typical variant of CIDP, with (23 patients) and without (37 patients) diabetes mellitus. We analysed the results of the neurophysiological tests, and correlated them with the leukocyte subpopulations, and cytokine levels. In CIDP patients, IL-6, IL-2, IL-4 and TNF-α levels were significantly increased compared to the control group. Fifty patients had decreased levels of T CD8+ lymphocytes, and 51 patients had increased levels of CD4+ lymphocytes. An increased CD4+/CD8+ ratio was also found. Negative correlations were observed mainly between compound muscle action potential (CMAP) amplitudes and cytokine levels. The study enabled the conclusion that electrophysiological parameters in CIDP patients are closely related to the autoimmune process, but without any clear differences between patients with and without diabetes mellitus. Correlations found in the study indicated that axonal degeneration might be independent of the demyelinating process and might be caused by direct inflammatory infiltration.

scholarly journals Pro- and Anti-Inflammatory Cytokines in the First Trimester—Comparison of Missed Miscarriage and Normal Pregnancy

2021 ◽  
Vol 18 (16) ◽  
pp. 8538
Author(s):  
Maciej Kwiatek ◽  
Tomasz Gęca ◽  
Anna Kwaśniewska
Keyword(s):  
Immune Response ◽  
Inflammatory Cytokines ◽  
First Trimester ◽  
Normal Pregnancy ◽  
Control Group ◽  
Study Group ◽  
Tnf Α ◽  
Cytokine Levels ◽  
Anti Inflammatory ◽  
Tgf Β1

The advantage in response of Th2 over Th1 is observed in normal pregnancy in peripheral blood. A disturbance of this balance can lead to symptoms of miscarriage and pregnancy loss. The aim of this study was to evaluate the pro- and anti-inflammatory cytokines in sera of women who were diagnosed with missed miscarriage in the first trimester and to compare this systemic immune response to the response in women with normal pregnancy. The study group consisted of 61 patients diagnosed with missed miscarriage. In total, 19 healthy women with uncomplicated first trimester created the control group. Cytokines were determined in the maternal serum by ELISA. The analysis included INF-γ, TNF-α, Il-1β, Il-4, Il-5, Il-6, Il-9, Il-10, Il-13 and TGF-β1. Th1 cytokine levels in the study group reached slightly higher values for INF-γ, Il-1β and slightly lower for IL-6 and TNF-α. In turn, Th2 cytokine levels in the study group were slightly higher (Il-9, Il-13), significantly higher (Il4, p = 0.015; Il-5, p = 0.0003) or showed no differences with the control group (Il-10). Slightly lower concentration involved only TGF-β1. Analysis of the correlation between levels of pro- and anti-inflammatory cytokines resulted in some discrepancies, without showing predominance of a specific immune response. The results did not confirm that women with missed miscarriage had an advantage in any type of immune response in comparison to women with normal pregnancy.

scholarly journals The expression and significance of inflammatory cytokines and MMP-9 in the tears of the infected eye and contralateral uninfected eye in patients with fungal keratitis

2021 ◽  
Author(s):  
Shuang Zhang ◽  
Hui-Min Wu ◽  
Xiang-Ni Cao ◽  
Xian-Qi Zhang ◽  
Gui-ping Gao
Keyword(s):  
Tear Film ◽  
Fungal Keratitis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Healthy Controls ◽  
Tnf Α ◽  
Cytokine Levels ◽  
Healthy Control ◽  
The Relationship ◽  
Tear Film Function

Abstract Background: We investigated bilateral tear cytokine levels including interleukin (IL)-1β, IL-10, IL-17, tumor necrosis factor (TNF)-α and Matrix metalloproteinase-9 (MMP-9) in patients with fungal keratitis(FK). Meanwhile, we evaluated the relationship between the changes of tear cytokines with corneal perception and pain in infected eyes, and the relationship between tear cytokines and tear film function in contralateral uninfected eyes .Methods : A total of 60(20 FK, 20 contralateral, 20 healthy controls) tear samples were collected prospectively and analyzed by enzyme linked immunosorbent assay(ELISA). Approximately 50 to 60 ul of tear samples in each case were collected. Meanwhile ,we analyzed the changes of visual analogue scale(VAS), tear breakup time (TBUT), Schirmer I test (SIT) and corneal perception compared with healthy controls. Results :The concentrations of IL-1β, IL-10 and IL-17 increased in bilateral eyes compared with healthy controls(P<0.05). The tear concentrations of MMP-9 , TNF-α only significantly increased in affected eyes (P <0.05). Patients with FK showed significant reduction in corneal perception of infected eyes compared with controls(P<0.05). Corneal perception of the normal eyes in FK patients was slightly lower than that of control group, but there was not statistical difference (P>0.05).TBUT and SIT of contralateral uninfected eyes were significantly lower than that of control group(P<0.05), which were significantly correlated with levels of IL-1β, IL-17(P<0.05). SIT were also negatively correlated with MMP-9(P<0.05), while the levels of IL-1β, IL-10, IL-17, TNF-α and MMP-9 in the tears of the healthy control group had no significant correlation with TBUT and SIT indicators(P>0.05).The corneal perception and VAS score of the affected FK eyes showed correlation with IL-1β, IL-17 and TNF-α(P<0.05).In addition, concentration of IL-10 inversely was correlated with VAS (P<0.05). Conclusion: Proinflammatory tear cytokines are elevated in bilateral eyes with unilateral FK as associated with tear film function ,pain and corneal sensitivity.

Lack of Evoked Laryngeal Electromyography Response in Patients with a Clinical Diagnosis of Vocal Cord Paralysis

2001 ◽  
Vol 110 (9) ◽  
pp. 815-819 ◽  
Author(s):  
Edward J. Damrose ◽  
Keith E. Blackwell ◽  
Robert Y. Huang ◽  
Joel A. Sercarz ◽  
Joel H. Blumin ◽  
...  
Keyword(s):  
Vocal Cord ◽  
Vocal Cord Paralysis ◽  
Compound Muscle Action Potential ◽  
Constant Current ◽  
Control Group ◽  
Vocal Cord Motion ◽  
Muscle Action Potential ◽  
Evoked Electromyography ◽  
Laryngeal Reinnervation ◽  
Recent Debate

There has been recent debate about whether patients with vocal cord immobility have a neurologic paralysis or whether synkinesis, the misdirection of axons to competing laryngeal muscles, is responsible for the lack of voluntary vocal cord motion. This issue was studied in 15 patients with vocal cord paralysis who underwent laryngeal reinnervation. Evoked electromyography was performed with a surface electrode endotracheal tube. The recurrent laryngeal nerve (RLN) was identified and stimulated with constant current. Of the 15 patients, only 1 produced a compound muscle action potential upon nerve stimulation. The remaining 14 patients had no evoked response during RLN stimulation. a control group of 8 patients with normal vocal cord mobility was studied, and each had a normal evoked electromyography response after RLN stimulation. These results support the assertion that patients who require treatment for vocal cord paralysis do not have synkinesis produced by RLN reinnervation.

scholarly journals Rosiglitazone Decreases Serum Bone-Specific Alkaline Phosphatase Activity in Postmenopausal Diabetic Women

2007 ◽  
Vol 92 (9) ◽  
pp. 3523-3530 ◽  
Author(s):  
Zehra Berberoglu ◽  
Alptekin Gursoy ◽  
Nilufer Bayraktar ◽  
Ayse Canan Yazici ◽  
Neslihan Bascil Tutuncu ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Bone Formation ◽  
Controlled Trial ◽  
Lifestyle Changes ◽  
Control Group ◽  
Long Term Effects ◽  
Open Label ◽  
Tnf Α ◽  
Cytokine Levels ◽  
Rosiglitazone Treatment

Abstract Objectives: Our objectives were to evaluate the effect of rosiglitazone on bone metabolism and to assess the association between changes in bone turnover parameters and plasma cytokine levels in postmenopausal diabetic women. Design: This was a 12-wk open-label randomized-controlled trial. Patients or Other Participants: A total of 56 obese postmenopausal women with newly diagnosed diabetes and 26 nondiabetic healthy controls matched for age and body mass index were included in the study. Interventions: The subjects were instructed to follow a weight-maintenance diet. Half were randomly assigned to receive rosiglitazone 4 mg/d, and the other half remained on diet alone. Main Outcome Measures: Before and after the interventions, metabolic bone markers and serum cytokine levels were assessed. Results: Serum total alkaline phosphatase (ALP) and bone-specific ALP levels were statistically significantly lower 12 wk after initiation of rosiglitazone treatment. There were no statistically significant changes in osteocalcin levels among the three groups or in deoxypyridinoline levels in the rosiglitazone group. At the end of 12 wk, all patients had statistically significantly decreased IL-1β and TNF-α levels compared with baseline. Changes in bone-specific ALP levels showed a moderate negative correlation with the changes in the TNF-α levels after rosiglitazone treatment and after diet in the diabetic control group. Conclusions: Rosiglitazone use is associated with reduced bone formation at earlier stages in postmenopausal diabetic women. The cytokine-lowering effects of rosiglitazone and lifestyle changes could reverse the early inhibitory effect of rosiglitazone therapy on bone formation. Further studies will clarify the long-term effects of rosiglitazone therapy on bone loss and fracture.

scholarly journals Anti-inflammatory activities of puerarin in high-fat diet-fed rats with streptozotocin-induced gestational diabetes mellitus

2020 ◽  
Vol 47 (10) ◽  
pp. 7537-7546 ◽  
Author(s):  
Wenting Xu ◽  
Mengyu Tang ◽  
Jiahui Wang ◽  
Lihong Wang
Keyword(s):  
Diabetes Mellitus ◽  
Insulin Resistance ◽  
Gestational Diabetes ◽  
Gestational Diabetes Mellitus ◽  
Density Lipoprotein ◽  
Reproductive Organs ◽  
Control Group ◽  
High Fat ◽  
Adipose Tissues ◽  
Tnf Α

Abstract To investigate the effect of puerarin on insulin resistance and inflammation in rats with gestational diabetes mellitus (GDM). Gestational diabetic model rats were established by intraperitoneal injection of streptozotocin (25 mg/kg) combined with high-fat feeding and were randomly assigned to three groups: the control group, the GDM group, and the puerarin-treated group. Puerarin was intragastrically administered to rats daily until the offspring were born. The rats in both the GDM group and control group were administered the same volume of normal saline. Serum total cholesterol, triglycerides, high-density lipoprotein cholesterol, and low-density lipoprotein cholesterol in all groups of rats were measured. Haematoxylin and eosin staining was used to evaluate morphological changes in the liver, pancreas, and adipose tissues around the reproductive organs. Western blotting was carried out to measure the protein expression of IRS-1 and inflammatory factors, including TNF-α, TLR4, MyD88 and phosphorylated NF-κB, in the adipose tissues around the reproductive organs. Puerarin had preventive effects on GDM-induced pathological changes and ameliorated glucose and lipid metabolism disorders in GDM rats. Puerarin upregulated IRS-1 expression and decreased the protein expression of TNF-α, TLR4, and MyD88 as well as the levels of phosphorylated NF-κB in adipose tissues around the reproductive organs in GDM rats. This study indicated that puerarin exerts anti-inflammatory effects by downregulating the important TLR4/MyD88/NF-κB inflammatory signalling pathway. Therefore, puerarin can decrease the expression of TNF-α and ameliorate insulin resistance in GDM rats, suggesting the potential efficacy of puerarin in GDM treatment.

scholarly journals Proinflammatory Cytokines Correlate with Depression and Anxiety in Colorectal Cancer Patients

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Diego Oliveira Miranda ◽  
Taís Aparecida Soares de Lima ◽  
Lucas Ribeiro Azevedo ◽  
Omar Feres ◽  
José Joaquim Ribeiro da Rocha ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Proinflammatory Cytokines ◽  
Molecular Mechanisms ◽  
Depression Scale ◽  
Serum Levels ◽  
Anxiety And Depression ◽  
Control Group ◽  
Depression And Anxiety ◽  
Tnf Α ◽  
Cytokine Levels

The objective of this study was to investigate whether serum cytokine levels correlate with depression and anxiety in colorectal cancer (CRC) patients. Twenty patients hospitalized for surgical resection of CRC were included in the study group and twenty healthy volunteers comprised the control group. Depression and anxiety were analyzed using the Hospital Anxiety and Depression Scale (HADS), and serum levels of IL-1β, IL-6, IL-8, IL-10, IL-12, TNF-α, and TGF-βwere measured by Cytometric Bead Array. We found that more than half of CRC patients presented clinically significant levels of anxiety or depression, and 65% of them manifested a combination of severe anxiety and depression. CRC patients had increased serum levels of IL-1β, IL-6, IL-8, and TNF-α but lower IL-10 concentrations. Correlation analysis between HADS score and cytokine levels revealed a positive association of anxiety and/or depression with IL-1β, IL-6, IL-8, and TNF-α and a negative correlation with IL-10. These results indicate that circulating proinflammatory cytokines are involved in the pathophysiology of anxiety and depression in CRC patients. A better understanding of the molecular mechanisms involved in these psychological disorders will allow the design of therapeutic interventions that lead to an improved quality of life and overall survival of CRC patients.

scholarly journals Effect of various inductions of sleep deprivation stress on proinflammatory cytokine levels in gingival crevicular fluids of white male Wistar strain rats (Rattus novergicus)

2020 ◽  
Vol 53 (1) ◽  
pp. 24
Author(s):  
Pratiwi Nur Widyaningsih ◽  
Fitranto Arjadi ◽  
Erlina Sih Mahanani
Keyword(s):  
Sleep Deprivation ◽  
Proinflammatory Cytokines ◽  
Paradoxical Sleep ◽  
White Male ◽  
Control Group ◽  
Total Sleep Deprivation ◽  
Wistar Strain ◽  
Tnf Α ◽  
Cytokine Levels ◽  
One Way Anova

Background: Stress that is induced by sleep deprivation can modulate the damage of periodontal tissue by elevating the levels of proinflammatory cytokines (i.e. IL-1β and TNF-α). The effects of sleep deprivation can be resolved with sleep recovery. Gingival crevicular fluid (GCF) is fluid in sulcular gingiva which acts as an oral biomarker for evaluating periodontal abnormalities. Purpose: The aim of this study was to determine the effect of various induction methods of sleep deprivation stress on cytokine levels in GCF of white male Wistar strain rats (Rattus novergicus). Methods: The study method was true experimental with a posttest-only control group design. Thirty male Wistar rats were randomly divided into five groups: paradoxical sleep deprivation (PSD), total sleep deprivation (TSD), partial sleep deprivation with sleep recovery for five days (PSD+SR), total sleep deprivation with sleep recovery for five days (TSD+SR) and a healthy control group. Data were analysed via one-way ANOVA to determine differences between groups. Result: The results showed the highest level of IL-1β and TNF-α was found in the PSD group. One-way ANOVA analysis showed significant differences (p<0,05) of IL-1β level between PSD and control groups, PSD and PSD+SR groups and PSD and TSD+SR groups; in contrast, the analysis of TNF-α levels showed significant differences (p<0,05) between PSD group to control group, PSD to PSD+SR group and TSD to TSD+SR group. Conclusions: There is an effect of various induction methods of sleep deprivation stress on proinflammatory cytokines (IL-1β and TNF-α).

scholarly journals Analysis on the Effect of Metformin Hydrochloride Combined with Insulin Pump for Gestational Diabetes Mellitus

2022 ◽  
Author(s):  
Xinghua Li ◽  
Guilian Li ◽  
Yan Liu ◽  
Fanchun Meng ◽  
Lihong Han ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Blood Glucose ◽  
Gestational Diabetes ◽  
Research Group ◽  
Pregnancy Outcomes ◽  
Insulin Pump ◽  
Control Group ◽  
Metformin Hydrochloride ◽  
Tnf Α ◽  
Adverse Pregnancy

Background: U To analyze the effect of metformin hydrochloride combined with insulin pump for gestational diabetes mellitus (GDM). Methods: Overall, 216 patients with GDM in Zhangqiu Maternity and Child Care Hospital, Jinan, China from Aug 2018 to Dec 2020 were enrolled and randomized into research and control groups. Patients in the control group were treated with insulin pump, while those in the research group were treated with metformin hydrochloride combined with insulin pump. The clinical efficacy, blood glucose levels, serum Betatrophin, C reactive protein (CRP), Cystatin C (Cys-C), homocysteine (Hcy), adiponectin, tumor necrosis factor (TNF-α), interleukin-6 (IL-6) content, incidence of adverse pregnancy outcomes and incidence of adverse newborns of patients in the two groups were compared. Results: After treatment, the total clinical efficiency of the research group was 84.26%, significantly higher than that of the control group (68.52%). The levels of FPG, 2hPG, HbAlc, serum Betatrophin, CRP, CysC, Hcy, adiponectin factors, TNF-α, and IL-6 in the research group were lower than those in the control group, with statistically significant differences (P<0.05). The overall incidence of adverse pregnancy outcomes was 10.19% in the research group, and 25.93% in the control group. The comparative differences between the two groups were statistically significant (P<0.05). The overall incidence of adverse newborns was 9.26% in the research group, and 21.30% in the control group. The comparative differences between the two groups were statistically significant as well (P<0.05). Conclusion: Metformin hydrochloride combined with insulin pump for GDM can significantly reduce blood glucose level, regulate serum protein factor levels, and improve adverse outcomes for mother and child, which deserves clinical promotion.   

scholarly journals Moringa Leaf Powder (Moringa oleifera) Decrease of Inflammation Plasma Cytokine of Pregnant Rats with Diabetes Mellitus

2021 ◽  
Vol 9 (A) ◽  
pp. 1043-1046
Author(s):  
Harry Kurniawan Gondo
Keyword(s):  
Diabetes Mellitus ◽  
Risk Factors ◽  
Dose Group ◽  
Moringa Oleifera ◽  
Normal Pregnancy ◽  
Positive Control ◽  
Control Group ◽  
Group 4 ◽  
Tnf Α ◽  
Leaf Powder

BACKGROUND: Preeclampsia is a major obstetric problem worldwide, especially in developing countries that can cause maternal and fetal morbidity and mortality that affect 2–10% of pregnancies worldwide. More than 4 million pregnant women each year develop preeclampsia and cause 15–20% of maternal deaths worldwide. There are several high-risk factors associated with preeclampsia. Diabetes mellitus is one of the risk factors for preeclampsia. Preeclampsia has been shown to have higher levels of Th1 (pro-inflammatory) products and lower levels of Th2 (anti-inflammatory) products compared to normal pregnancy in blood serum. Interleukin (IL) 1, IL-2, IL-8, tumor necrosis factor (TNF)-alpha, and interferon gamma are Th1 cytokines or pro-inflammatory cytokines that can induce inflammatory reactions and are associated with pregnancy complications such as repeated abortion, preterm labor, rupture of membranes. preeclampsia, and stunted fetal growth. In contrast, Th2 cytokines such as transforming growth factor-β (TGF-β), IL-4, IL-5, IL6, and IL-10 are associated with normal pregnancy. AIM: This study aims to prove the effect of giving Moringa leaf powder (M. oleifera) to decrease blood levels, , so this study will answer the effect of Moringa oleifera leaf powder on blood level of IL-6, IL-10, TNF-α and TGF-β METHODS: A total of 30 rats were checked for sugar levels on day 4 after being induced by alloxan for 18 days to see if the rats were already in a hyperglycemic state. Then, they were divided into six groups, each group contained five pregnant white rats. Group 1 was the negative group (without being given alloxan), Group 2 was positive (given alloxan 150 mg/day/kg BW), and Groups 3, 4, 5, and 6 were given alloxan 150 mg/day/kg BW and each given a dose of 100 Moringa leaf powder; 200, 400, and 800 mg/day/kg body weight. After alloxan was induced and given a dose of Moringa leaf powder according to each group, blood samples were taken to separate the serum and continued with the ELISA method to calculate the levels of IL-6, IL-10, TNF-α, and TGF-β. RESULTS: The administration of Moringa leaf powder at a dose of 800 mg/day/kg BW was able to increase the levels of IL-10 in the positive control Group 4 by 7.211 mIU/ml and reduced levels of IL-6 in the 8th dose group by giving Moringa leaf powder as much as 800 mg/day/kg body weight by 2.112 mIU/ml, was able to increase TGF-β levels in the dose Group 4 by giving Moringa leaf powder as much as 800 mg/day/kg BW, amounted to 1049.066 mIU/ml, and decreased TNF-α levels in the dose Group 4 by giving Moringa leaf powder as much as 800 mg/day/kg BW mIU/ml. CONCLUSION:The administration of Moringa leaf powder was able to increase the levels of IL-10 in the positive control Group, reduced levels of IL-6, was able to increase TGF-β levels, and decreased TNF-α levels.

scholarly journals Cortical Excitability in Amyotrophic Lateral Sclerosis: A Clue to Pathogenesis

1993 ◽  
Vol 20 (1) ◽  
pp. 11-16 ◽  
Author(s):  
Andrew Eisen ◽  
Bhanu Pant ◽  
Heather Stewart
Keyword(s):  
Amyotrophic Lateral Sclerosis ◽  
Cortical Excitability ◽  
Motor Evoked Potentials ◽  
Compound Muscle Action Potential ◽  
Control Group ◽  
Normal Threshold ◽  
Hand Muscles ◽  
Muscle Action Potential ◽  
Disease Threshold ◽  
Lateral Sclerosis

ABSTRACT:Motor evoked potentials (MEPs) were recorded from selected non-wasted, non-denervated hand muscles in 40 patients with Amyotrophic Lateral Sclerosis (ALS) with both upper and lower motor neuron signs. In most the compound muscle action potential (CMAP) of the target muscle was normal. Compared to the control group, cortical threshold in ALS varied considerably and there was a significant (r2= 0.702) inverse, exponential, correlation between cortical threshold and MEP/CMAP ratio. There was a linear correlation between threshold and disease duration (r2= 0.66) so that early in the disease threshold was normal and later the motor cortex could not be stimulated. It is suggested that early in ALS normal threshold reflects glutamate-induced hyper-excitability of the corticomotoneuron. The findings lend support to the hypothesis that ALS is primarily a disease of the corticomotoneuron.

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