scholarly journals Effects of Aging Torque Controllers on Screw Tightening Force and Bacterial Micro-Leakage on the Implant-Abutment Complex

Materials ◽  
2022 ◽  
Vol 15 (2) ◽  
pp. 620
Author(s):  
Yousef Jiries ◽  
Tamar Brosh ◽  
Shlomo Matalon ◽  
Vladimir Perlis ◽  
Zeev Ormianer
Keyword(s):  
Polymerase Chain Reaction Test ◽  
Tightening Force ◽  
Reduction In Force ◽  
Implant Abutment ◽  
Before And After ◽  
Spring Type ◽  
Polymerase Chain ◽  
Bacterial Leakage ◽  
Effects Of Aging ◽  
Chain Reaction Test

Aim: We assess the accuracy of torque controllers after several aging processes and the bacterial leakage on Implant-Abutment complexes (IAC).Methods: A total of 12 spring-type and 12 friction-type torque controllers and 48 IAC (24 conical and 24 hexagonal connections) were evaluated. Chemical, mechanical, temperature, and pressure-aging methods were applied individually to replicate clinical use. Torque controller accuracy was analyzed before and after aging using a calibrated gauge. To assess bacterial leakage, the IAC were suspended in a bacterial medium for 24 h. Direct Contact Test (DCT) and Polymerase Chain Reaction Test (RT-PCR) analyzed the infiltration of F. nucleatum and P. gingivalis into the IAC micro-gap. Results: A significant decrease in torque after 10 days of aging was found. The spring-type torque controller was affected the most, regardless of the aging method (P < 0.05). PCR results indicated that all groups exhibited significantly more bacterial leakage, regardless of the method used (P < 0.05). The conical IAC demonstrated more bacterial leakage of P. gingivalis compared with the hexagonal IAC (P = 0.07). DCT found bacterial growth in the IAC only before aging and was not identified after aging. Conclusion: Aging affects torque accuracy. A reduction in force was noticed after 10 days. The conical IAC exhibits more bacterial leakage, although this was not statistically significant.

scholarly journals Use of Public Transport and Contraction of SARS-CoV-2 in a Large Prospective Cohort in Norway.

2021 ◽  
Author(s):  
Merete Ellingjord-Dale ◽  
Karl Trygve Kalleberg ◽  
Mette Istre ◽  
Anders B. Nygaard ◽  
Sonja H. Brunvoll ◽  
...  
Keyword(s):  
Public Transport ◽  
Public Transportation ◽  
Polymerase Chain Reaction Test ◽  
Calendar Time ◽  
Frequency Of Use ◽  
Before And After ◽  
Polymerase Chain ◽  
Chain Reaction Test ◽  
Incident Cases

Abstract Background: For many people public transport is the only mode of travel, and it can be challenging to keep the necessary distances in such a restricted space. The exact role of public transportation and risk of SARS-CoV-2 transmission is not known. Methods: Participants (n=121 374) were untested adult Norwegian residents recruited through social media who in the spring of 2020 completed a baseline questionnaire on demographics and use of public transport. Incident cases (n=1069) had a positive SARS-CoV-2 polymerase chain reaction test registered at the Norwegian Messaging System for Infectious Diseases by January 27, 2021. We investigated the association between use of public transport and SARS-CoV-2 using logistic regression. Odds ratios (ORs) with 95% confidence intervals (CIs) adjusted for age, calendar time, gender, municipality, smoking, income level, fitness and underlying medical conditions were estimated. Frequency of use of public transport was reported for 2 week-periods. Results: Before lockdown, those who tested positive on SARS-CoV-2 were more likely to have used public transport 1-3 times (OR =1.28, CI 1.09-1.51), 4-10 times (OR=1.49, CI 1.26-1.77) and ≥11 times (OR=1.50, CI 1.27-1.78, p for trend<0.0001) than those who had not tested positive. Conclusion: Use of public transport was positively associated with contracting SARS-CoV-2 both before and after lockdown.

scholarly journals Granulocytic anaplasmosis in captive ring-tailed lemur (Lemur catta) in Poland

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Łukasz Adaszek ◽  
Anna Wilczyńska ◽  
Jerzy Ziętek ◽  
Marcin Kalinowski ◽  
Oliwier Teodorowski ◽  
...  
Keyword(s):  
Anaplasma Phagocytophilum ◽  
Polymerase Chain Reaction Test ◽  
Anaplasma Ovis ◽  
Case Presentation ◽  
Granulocytic Anaplasmosis ◽  
Pcr Product ◽  
Polymerase Chain ◽  
Obligate Intracellular Bacteria ◽  
Chain Reaction Test ◽  
Anaplasma Bovis

Abstract Background Anaplasma are obligate intracellular bacteria and aetiological agents of tick-borne diseases of both veterinary and medical interest. The genus Anaplasma comprises six species: Anaplasma marginale, Anaplasma centrale, Anaplasma ovis, Anaplasma phagocytophilum, Anaplasma bovis and Anaplasma platys. They can infect humans, carnivores, ruminants, rodents, insectivores, birds and reptiles. The aim of this study was to present the first clinical case of granulocytic anaplasmosis in a captive ring-tailed lemur in Poland. Case presentation A 4-year-old female lemur presented anorexia, epistaxis and tick infestation. The microscopic examination of a blood smear revealed morulae in neutrophils. Polymerase chain reaction test and sequencing of obtained PCR product confirmed infection by the GU183908 Anaplasma phagocytophilum strain. Therapeutic protocol included doxycycline (2.5 mg/kg p.o., b.i.d.) for 3 weeks and the lemur recovered within 24 h. Conclusions This is the first report on granulocytic anaplasmosis in a ring-tailed lemur in Europe, indicating that A. phagocytophilum infection must also be considered in differential diagnosis in this animal species, especially in individuals with thrombocytopenia associated with Ixodes ricinus parasitism.

scholarly journals Clinical Evaluation of a 16S Ribosomal RNA Polymerase Chain Reaction Test for the Diagnosis of Lymph Node Tuberculosis

2006 ◽  
Vol 43 (7) ◽  
pp. 855-859 ◽  
Author(s):  
Fernando Osores ◽  
Oscar Nolasco ◽  
Kristien Verdonck ◽  
Jorge Arevalo ◽  
Juan Carlos Ferrufino ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Lymph Node ◽  
Rna Polymerase ◽  
Ribosomal Rna ◽  
Polymerase Chain Reaction Test ◽  
Chain Reaction ◽  
Reaction Test ◽  
Polymerase Chain ◽  
Lymph Node Tuberculosis ◽  
Chain Reaction Test

Polymerase Chain Reaction for the Direct Detection of Brucella spp. in Milk and Cheese

2001 ◽  
Vol 64 (2) ◽  
pp. 164-167 ◽  
Author(s):  
G. TANTILLO ◽  
A. DI PINTO ◽  
A. VERGARA ◽  
C. BUONAVOGLIA
Keyword(s):  
Polymerase Chain Reaction ◽  
Direct Detection ◽  
Polymerase Chain Reaction Test ◽  
Chain Reaction ◽  
Sheep Milk ◽  
Reaction Test ◽  
Polymerase Chain ◽  
Quality Of Milk ◽  
Chain Reaction Test

A polymerase chain reaction test was developed to detect Brucella spp. directly in milk and cheese and optimized using primers for the BSCP-31 gene. A total of 46 cheese samples produced with sheep and goats milk were assayed, and Brucella spp. was detected in 46% of them, especially in cheese made from sheep milk. This method is of remarkable epidemiologic interest because it is an indirect test indicating the sanitary quality of milk used in dairy industries. The method showed good sensitivity and specificity. It is faster and less expensive than the conventional bacteriological assays.

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