scholarly journals Characterization of Novel Selected Microalgae for Antioxidant Activity and Polyphenols, Amino Acids, and Carbohydrates

Marine Drugs ◽  
2021 ◽  
Vol 20 (1) ◽  
pp. 40
Author(s):  
Paula Santiago-Díaz ◽  
Argimiro Rivero ◽  
Milagros Rico ◽  
Juan Luis Gómez-Pinchetti
Keyword(s):  
Amino Acids ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Essential Amino Acids ◽  
Butylated Hydroxytoluene ◽  
Soluble Carbohydrates ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Dry Biomass

The biochemical composition of three novel selected microalgae strains (Chlorophyta) was evaluated to confirm their potential possibilities as new sustainably produced biomass with nutritional, functional, and/or biomedical properties. Extracts from cultured Pseudopediastrum boryanum, Chloromonas cf. reticulata, and Chloroidium saccharophilum exhibited higher radical scavenging activity of DPPH (1,1-diphenyl-2-picrylhydrazyl) when compared to butylated hydroxytoluene (BHT), but lower than butylated hydroxyanisole (BHA). Total phenolic compounds and amino acids were determined by newly developed RP-HPLC methods. Total phenolic contents, as µg g−1 of dry biomass, reached 27.1 for C. cf. reticulata, 26.4 for P. boryanum, and 55.8 for C. saccharophilum. Percentages of total analysed amino acids were 24.3, 32.1, and 18.5% of dry biomass, respectively, presenting high values for essential amino acids reaching 54.1, 72.6, and 61.2%, respectively. Glutamic acid was the most abundant free amino acid in all microalgae samples, followed by proline and lysine in C. saccharophilum and P. boryanum, and methionine and lysine in C. reticulata. Soluble carbohydrates in aqueous extracts ranged from 39.6 for C. saccharophilum to 49.3% for C. reticulata, increasing values to 45.1 for C. saccharophilum and 52.7% for P. boryanum in acid hydrolysates of dried biomass. Results confirmed the potential possibilities of these microalgae strains.

scholarly journals Antioxidant and beta-Galactosidase Inhibitory Studies of Methanolic Extract of Juniperus Phoenicea and Calicotome Villosa from Jordan

2021 ◽  
Author(s):  
Ahmed Al-Mustafa ◽  
Mohammad Al-Tawarah ◽  
Mohammed Sharif Al-Sheraideh ◽  
Fatima Attia Al-Zahrany
Keyword(s):  
Thermodynamic Parameters ◽  
Thermal Inactivation ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Antioxidant Power ◽  
Dry Extract ◽  
Phenolic Contents ◽  
Juniperus Phoenicea

Abstract Background: We investigated Juniperus Phoenicea (J. Phoenicea) and Calicotome Villosa (C. Villosa) from Jordan for phenolic contents, antioxidant, anti β-Galactosidase activities, in an attempt to rationalize its use in lactose metabolism disorders. The kinetic parameters of leave extracts, galactose, glucose, fructose and acarbose were evaluated. Also, the thermodynamic parameters of the enzyme thermal inactivation were determined. Methods: JP and cv crude methanolic extracts were evaluated for 1,1-diphenyl,2-picrylhydrazyl (DPPH) free radical scavenging activity and ferric reducing antioxidant power (FRAP). Further, β-Galactosidase inhibitory activities were performed using O-nitrophenyl-beta-D-galactopyranoside as substrate. Moreover, total phenolic contents, flavonoids and flavonols of plants extracts were determined and expressed in mg of gallic acid equivalent (mg GAE/g dry extract) or rutin equivalent per gram of dry extract (mg RE/g dry extract).Results: Phytochemical screening of the crude extract of J. Phoenicea and C. Villosa leaves contained phenols, alkaloids, flavonoids, terpenoids, anthraquinones and glycosides. J. Phoenicea exhibited high flavonoids and flavonols contents than C. Villosa but both J. Phoenicea and C. Villosa contained high phenolic and showed concentration dependent DPPH scavenging activity, with J. Phoenicea (IC50 =11.1 μg/ml), C. Villosa (IC50 =15.6 μg/ml), respectively. According to FRAP assay, the antioxidant power activity of plants extracts was concentrations dependent. The β-galactosidase % inhibition was increased as the concentration of of J. phoenicea, C. villosa and rutin increased. The mode of inhibition of β-galactosidase by J. phoenicea (IC50= 65 µg/ml) and C. villosa (IC50= 700 µg/ml) extracts was non-competitive and mixed-inhibition, respectively. Also, rutin was affected in a competitive (IC50 = 75 µg/ml) inhibition. β-galactosidase half-life was 108 min at 55°C, thermodynamic parameters revealed an activation energy of 208.88 kJ mol-1 and the inactivation kinetic follows a first-order reaction with k-values ranges between 0.0862 and 0.0023 min-1. The enzyme showing a decreasing trend of enthalpy of denaturation (∆H°) as temperature increase but value of free energy of thermal denaturation (∆G°) for β-galactosidase was decreased with increasing in temperature. The calculated entropy of inactivation (∆S°) at each temperature showed positive values, which means there are no significant processes of aggregation.Conclusions: J.phoenicea and C.villosa have inhibiting effect on β-galactosidase activity. Thermodynamic approach shows an enzyme stable and suggests that inactivation mechanism is based on molecular structural changes.

scholarly journals Antioxidant, a-Amylase Inhibitory Activities and Photoprotective Properties of Peels of Nephelium Lappaceum Linn. (Malwana Special)

2021 ◽  
Vol 37 (2) ◽  
pp. 499-507
Author(s):  
Amugoda Kankanamge Dona Malsha Binuwangi ◽  
Madinage Pawani Jayathri Perera ◽  
Asurumunige Anushka Gayathrie Silva ◽  
Renuka Nilmini Attanayake ◽  
Chandima Shashikala Kumari Rajapakse
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Butylated Hydroxytoluene ◽  
Total Phenolic ◽  
Aqueous Methanol ◽  
Methanol Fraction ◽  
Standard Drug ◽  
Nephelium Lappaceum ◽  
Inhibitory Activities ◽  
Promising Source

This study focused on evaluation of antioxidant, α-amylase inhibitory activities and photo protective properties of peels of Nephelium lappaceum Linn. (rambutan); Malwana special. Methanolic extract of peels was sequentially partitioned in hexane, dichloromethane (DCM) and aqueous methanol. The methanol extract showed a significantly (p < 0.05) higher DPPH radical scavenging activity than that of butylated hydroxytoluene. Among the fractions, the highest total phenolic content (TPC) was found in the aqueous methanol fraction. DCM and aqueous methanol fractions were rich in flavonoids. In vitro α-amylase inhibitory activity of the aqueous methanol fraction was also significantly higher than the standard drug, acarbose. Partially purified aqueous methanol fraction of rambutan peels exhibited UV-B absorption with a moderate solar protection factor. The results revealed that the peels of Nephelium lappaceum Linn., Malwana specialcan be considered as a promising source for the development of natural antioxidant, cosmeceutical sunscreen and antidiabetic agents.

scholarly journals Chemical Composition and Antioxidant Activity of the Essential Oil and Fatty Acids of the Flowers of Rhanterium Adpressum

2013 ◽  
Vol 8 (8) ◽  
pp. 1934578X1300800 ◽  
Author(s):  
Chahrazed Hamia ◽  
Nadhir Gourine ◽  
Hadjer Boussoussa ◽  
Mokhtar Saidi ◽  
Emile M. Gaydou ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Essential Oil ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Lipid Extract ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Total Phenolic Contents ◽  
Oil Extract

The essential oil obtained by hydrodistillation of the flowers of Rhanterium adpressum Coss. & Durieu was analyzed using GC and GC-MS. The essential oil was very rich in monoterpene compounds. The major components identified were the monoterpene hydrocarbons: camphene (21.8%), myrcene (19.3%) and α-pinene (17.4%). Other compounds, including limonene, β-pinene and terpinol-4-ol, were present in low content (4–6%). The composition of the fatty acids in the lipid extract obtained from the flowers was also investigated by GC and GC-MS. The main fatty acids identified were palmitic (47.4%), oleic (12.9%) and stearic acids (10.6%). The total phenolic contents and the antioxidant activities were also evaluated for both extracts. The total phenolic contents were determined using the Folin-Ciocalteu reagent and the antioxidant activities were measured using three different assays: DPPH (1,1-diphenyl-2-picrylhydrazyl) free radical scavenging activity, FRAP (ferric reducing antioxidant potential) and a molybdenum assay. As a result of these tests, the lipid extract exhibited the highest antioxidant activities in comparison with the essential oil extract.

scholarly journals Polyphenolic Contents and Antioxidant Properties of Different Grape (V. vinifera,V. labrusca, andV. hybrid) Cultivars

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Shivraj Hariram Nile ◽  
S. H. Kim ◽  
Eun Young Ko ◽  
Se Won Park
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Coumaric Acid ◽  
Grape Skin ◽  
Phenolic Contents ◽  
Grape Skins ◽  
Phenolic And Flavonoid

The polyphenolic contents and the antioxidant activity of the skins and pulps of different grape cultivars were estimated using HPLC and DPPH antioxidant assay, respectively. The phenolics and flavonoids identified were quercetin, kaempferol, caffeic acid, p-coumaric acid, cinnamic acid, and (−)-epicatechin. The total phenolic contents were found to be the highest in the grape skin of Flouxa (>400 mg/100 g), followed by Campbell Early and Tamnara (>300 mg/100 g), and then by Red Globe and Ruby Seedless (>250 mg/100 g), and the total phenolic content was the lowest in Italia and Delaware (<60 mg/100 g). The antioxidant activities of the grape extracts varied from 12.5% (Ruby Seedless) to 60.2% (Hongiseul) for skins, whereas the antioxidant activities of the grape extracts varied from 35.4% (Campbell Early) to 84.5% (Hongiseul) for pulps. The grape pulps have stronger antioxidant activities than those of the grape skins. Our results suggest that the phenolic and flavonoid contents in extracts of grape skins and pulps showed statistically significant correlations with the free radical scavenging activity.

scholarly journals In vitro assessment of the protection from oxidative stress by various fractions of Artemisia incisa Pamp.

2011 ◽  
Vol 76 (10) ◽  
pp. 1379-1386
Author(s):  
A Aziz-Ur-Rehman ◽  
Sehrish Gulzar ◽  
Muhammad Abbasi ◽  
Tayyaba Shahzadi ◽  
Tauheeda Riaz ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Butylated Hydroxytoluene ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Aqueous Fraction ◽  
Antioxidant Power ◽  
Ic50 Value

The methanolic extract of Artemisia incisa Pamp. was dissolved in distilled water and successively partitioned with n-hexane, chloroform, ethyl acetate and n-butanol. The antioxidant potential of all these fractions and remaining aqueous fraction was evaluated by four methods, i.e., the scavenging activity of the 1,1-diphenyl-2-picrylhydrazyl radical (DPPH), the total antioxidant activity, the ferric reducing antioxidant power (FRAP) assay and the ferric thiocyanate assay. In addition, the total phenolics was determined. The obtained results revealed that among the studied fractions the ethyl acetate soluble fraction showed the most potent DPPH-radical scavenging activity with an IC50 value of 5.3 ? 0.71 ?g mL-1, which is even more effective than the standard antioxidant butylated hydroxytoluene (BHT) (IC50 value 0f 12.1? 0.92 ?g mL-1). The ethyl acetate fraction also showed the highest FRAP value (3677.13 ? 27.1 ?g TE mL-1), inhibition of lipid peroxidation (60.93 ? 0.84 % at 500 ?g mL-1) and total phenolic content (95.5 ? 0.05 ?g GAE g-1) as compared to other fractions. However, the remaining aqueous fraction was found to posses the highest antioxidant activity of all the fractions.

scholarly journals Cultivar effect on the sweet cherry antioxidant and some chemical attributes

2016 ◽  
Vol 28 (1) ◽  
pp. 95-102 ◽  
Author(s):  
Jan Skrzyński ◽  
Małgorzata Leja ◽  
Aleksander Gonkiewicz ◽  
Przemysław Banach
Keyword(s):  
Organic Acids ◽  
Sweet Cherry ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Soluble Solids ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Chemical Attributes ◽  
Sweet Cherry Cultivars

Abstract This research was carried out to evaluate the phenolic composition, antioxidant capacities, sugars and organic acids content of sweet cherry cultivars (Bianca, Burlat, Johanna 1, Johanna 2, Kordia, Kunzes Kirsche, Merton Premier, Napoleon, Orleans, Regina, Rivan, Schneiders Spate Knorpelkirsche, Summit, Trebnitzer Lotkirsche) grown in Poland. Significant differences were observed between tested cultivars for all studied parameters. The sum of total soluble solids ranged from 14.3 (‘Burlat’) to 20.9 g 100 g−1 FW (‘Bianca’) and that of organic acids from 0.43 (‘Burlat’) to 0.76 g 100 g−1 FW (‘Napoleon’). Fruit of Bianca cultivar showed the highest levels of anthocyanin (108.5 mg 100 g−1 FW) while ‘Napoleon’ and ‘Kunzes Kirsche’ contained the lowest levels (1.5 and 1.8 mg 100 g−1 FW, respectively). Total phenolic contents ranged from 101 (‘Napoleon’) to 558 (‘Bianca’) mg 100 g−1 FW, tartaric esters from 26.2 (‘Summit’) to 66.5 (‘Bianca’) mg 100 g−1 FW and flavonoids from 7.9 (‘Summit’) to 49.1 (‘Bianca’) mg 100 g−1 FW. Bianca cultivar has also the highest free radical scavenging activity assayed by ABTS and DPPH methods 88 and 90% respectively.

scholarly journals Protein Bread Fortification with Cumin and Caraway Seeds and by-Products Flour

2018 ◽  
Author(s):  
Bouchra Sayed Ahmad ◽  
Thierry Talou ◽  
Evita Straumite ◽  
Martins Sabovics ◽  
Zanda Kruma ◽  
...  
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Total Phenolic ◽  
Nutritional Values ◽  
Phenolic Contents ◽  
Trolox Equivalent ◽  
Sensory Color ◽  
By Products

This study investigated the effect of protein bread fortification with 2, 4 and 6% of cumin (Cuminum cyminum) and caraway (Carum carvi) whole seeds and by-products flour, respectively. Fortified protein bread samples were compared to control protein bread and evaluated for their sensory, color, moisture, hardness properties as well as their nutritional values. Total phenolic contents and Trolox equivalent antioxidant capacity were also analyzed. Results indicated that bread fortification shows significant effects on bread properties depending on fortification level. A higher acceptability was observed specially for bread fortified with by-products flour. Increased tendencies of color darkness, moisture content, bread hardness, nutritional values as well as total phenolic content and radical scavenging activity compared to control bread were observed as the percentage of fortification increased in both cases. The overall results showed that addition of cumin and caraway seeds and by-products flour can improve the antioxidant potential and overall quality of protein bread.

PHYTOCHEMICAL SCREENING, TOTAL PHENOLIC AND TOTAL FLAVONOID CONTENT, AND ANTIOXIDANT ACTIVITY OF DIFFERENT PARTS OF Melastoma malabathricum

2015 ◽  
Vol 77 (2) ◽  
Author(s):  
Suleiman Danladi ◽  
Amirah Wan-Azemin ◽  
Yahaya Najib Sani ◽  
Khamsah Suryati Mohd ◽  
Mahadeva Rao US ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Flavonoid Content ◽  
Phenolic Contents ◽  
Melastoma Malabathricum ◽  
Different Parts

Melastoma malabathricum Linn. commonly known as ‘senduduk’ in Malaysia, belongs to Melastomataceae family. The study was carried out to screen the phytochemicals present in methanol extract of leaf, flower, fruit, and stem and to evaluate the antioxidant activity, total flavonoid and total phenolic contents of these different parts. Phytochemical screening showed that all parts of this plant contained tannins, steroids, phenols and flavonoids. The flower extract was found to have highest total phenolic whereas the leaf demonstrated the highest flavonoid content followed by flower. The results of antioxidant activity from the DPPH assay showed that the flower has the highest radical scavenging activity comparable to quercetin standard while stem showed the lowest activity. The higher content of total phenolics, flavonoids and antioxidant capacity of flower suggests the possibility of its incorporation and exclusion of stem in M. malabathricum preparations for development of newer effective drugs.

scholarly journals Antioxidative and Quality Properties of Full-Fat Date Seeds Brew as Influenced by the Roasting Conditions

Antioxidants ◽  
2019 ◽  
Vol 8 (7) ◽  
pp. 226 ◽  
Author(s):  
Mohammad Fikry ◽  
Yus Aniza Yusof ◽  
Alhussein M. Al-Awaadh ◽  
Russly Abdul Rahman ◽  
Nyuk Ling Chin ◽  
...  
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Extraction Yield ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Browning Index ◽  
Phenolic Contents ◽  
Dpph Radical Scavenging

Full-fat roasted date seeds are considered an excellent source of antioxidants which can treat many diseases. The specific objectives were to investigate the effect of roasting temperature and time on the hardness of whole seeds, moisture content of the roasted date seeds powder, DPPH radical scavenging activity, total phenolic contents, extraction yield, pH, browning index and sensory properties of the brew prepared from the full-fat roasted date seeds and to construct descriptive models that could describe this effect. Date seeds were roasted at three temperatures (160, 180 and 200 °C) for different period of times (10, 20 and 30 min) using a natural conventional oven; then grinded and next brewed. Hardness of whole seeds, moisture content of the seeds powder, DPPH radical scavenging activity and total phenolic contents, extraction yield, pH and browning index and sensory properties of the brew were significantly affected by the roasting conditions. The statistical results indicated that the proposed model could adequately describe the measured properties. Strong correlations have been found among the properties of the brew as well. The producers of the date seeds brew can utilize these results for controlling the roasting process.

scholarly journals Propolis Extract as Antioxidant to Improve Oxidative Stability of Fresh Patties during Refrigerated Storage

Foods ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 614 ◽  
Author(s):  
Rey Vargas-Sánchez ◽  
Gastón Torrescano-Urrutia ◽  
Brisa Torres-Martínez ◽  
Mirian Pateiro ◽  
José Lorenzo ◽  
...  
Keyword(s):  
Oxidative Stability ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Ethanol Extract ◽  
Butylated Hydroxytoluene ◽  
Total Phenolic ◽  
Propolis Extract ◽  
Pork Patties

The effect of propolis ethanol extract (PEE), butylated hydroxytoluene (BHT), and ascorbic acid (Asc) against lipid (Lox) and protein oxidation (Pox), color deterioration, and the antioxidant stabilizer of raw beef and pork patties during chilled storage (9 days at 2 °C/under darkness) was investigated. Total phenolic content (TPC), reducing power ability (RPA), DPPH● radical scavenging activity (FRSA) of the PEE was evaluated. Meat samples were evaluated for pH, Lox (TBARS), Pox (Carbonyls), color (L*, a*, b*, C*, and h*), metmyoglobin formation (MMb), TPC, RPA, and FRSA. Results indicated that PEE is rich in phenolic content and antioxidant activity, and their incorporation in beef and pork patties reduced (p < 0.05) Lox and Pox (TBARS-88.7 and 80% inhibition; Pox-47.3 and 30.6% inhibition, respectively), as well as loss of color and increased the oxidative stability throughout storage.

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