scholarly journals Development of Highly Sensitive Immunosensor for Detection of Staphylococcus aureus Based on AuPdPt Trimetallic Nanoparticles Functionalized Nanocomposite

Micromachines ◽  
2021 ◽  
Vol 12 (4) ◽  
pp. 446
Author(s):  
En Han ◽  
Yun Zhang ◽  
Jianrong Cai ◽  
Xinai Zhang
Keyword(s):  
Staphylococcus Aureus ◽  
Catalytic Performance ◽  
High Specific Surface Area ◽  
Electrochemical Immunosensor ◽  
Sensitive Detection ◽  
Label Free ◽  
Catalytic Current ◽  
Highly Sensitive ◽  
Label Free Detection ◽  
Growth Method

The rapid and sensitive detection of Staphylococcus aureus (S. aureus) is essential to ensure food safety and protect humans from foodborne diseases. In this study, a sensitive and facile electrochemical immunosensor using AuPdPt trimetallic nanoparticles functionalized multi-walled carbon nanotubes (MWCNTs-AuPdPt) as the signal amplification platform was designed for the label-free detection of S. aureus. The nanocomposite of MWCNTs-AuPdPt was prepared by an in situ growth method of loading AuPdPt trimetallic nanoparticles on the surface of MWCNTs. The synthesized MWCNTs-AuPdPt featured good conductivity and superior catalytic performance for hydrogen peroxide. The nanocomposite of MWCNTs-AuPdPt with good biocompatibility and high specific surface area was further functionalized by anti-S. aureus antibodies. The immobilized antibodies could efficiently capture S. aureus to the modified electrode by an immune reaction, which resulted in the change of catalytic current intensity to realize the sensitive detection of S. aureus. The designed immunosensor could detect S. aureus in a linear range from 1.1 × 102 to 1.1 × 107 CFU mL−1 with a low detection limit of 39 CFU mL−1. Additionally, the proposed immunosensor was successfully applied to determine S. aureus in actual samples with acceptable results. This strategy provided a promising platform for highly sensitive determination of S. aureus and other pathogens in food products.

scholarly journals Fabrication of a label-free electrochemical immunosensor using a redox active ferrocenyl dendrimer

2016 ◽  
Vol 40 (11) ◽  
pp. 9046-9053 ◽  
Author(s):  
Sudeshna Chandra ◽  
Christian Gäbler ◽  
Christian Schliebe ◽  
Heinrich Lang ◽  
Dhirendra Bahadur
Keyword(s):  
Pamam Dendrimer ◽  
Electrochemical Immunosensor ◽  
Sensitive Detection ◽  
Label Free ◽  
Amperometric Immunosensor ◽  
Redox Active ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

An amperometric immunosensor based on a redox active ferrocenyl end-grafted PAMAM dendrimer provides highly sensitive detection of immunoglobulin, down to 2 ng mL−1.

Gold nanoparticle integrated artificial nanochannels for label-free detection of peroxynitrite

2021 ◽  
Author(s):  
Jing Wu ◽  
Xing Wang ◽  
Lei Ge ◽  
Rui Lv ◽  
Fan Zhang ◽  
...  
Keyword(s):  
Small Molecules ◽  
Gold Nanoparticle ◽  
Sensitive Detection ◽  
Label Free ◽  
Highly Sensitive ◽  
Label Free Detection ◽  
Highly Sensitive Detection

A label-free method for rapid and highly sensitive detection of ONOO− was proposed by employing ABEI@AuNPs integrated nanochannels. This work paves a new way to develop a versatile platform for the detection of different biological small molecules.

Electrochemical Study of Dimensional Specific Carbon Nanomaterials Modified Glassy Carbon Electrode for Highly Sensitive Label-free Detection of Immunoglobulin A

2020 ◽  
Vol 16 (7) ◽  
pp. 833-842
Author(s):  
Juthi Adhikari ◽  
Mohammad Rizwan ◽  
David Koh ◽  
Natasha Ann Keasberry ◽  
Minhaz Uddin Ahmed
Keyword(s):  
Electric Current ◽  
Glassy Carbon Electrode ◽  
Glassy Carbon ◽  
Carbon Nanomaterials ◽  
Immunoglobulin A ◽  
Electrochemical Immunosensor ◽  
Carbon Electrode ◽  
Label Free ◽  
Highly Sensitive ◽  
Label Free Detection

Background: Immunoglobulin A (IgA) accounts for 15% of total protein production per day and plays a crucial role in the first-line immune defence. Recently, IgA has been established as a vital clinical biomarker for nephropathy, allergic asthma, celiac disease (CD), pneumonia, and asthma as well as some neurological disorders. In this work, we have studied several carbon nanomaterials (CNMs) having different dimensions (D): carbon nano-onions (CNOs) - 0D, single-walled carbon nanotubes (SWCNTs) - 1D, and graphene nanoplatelets (GNPs) - 2D, on glassy carbon electrode (GCE) to identify which CNMs (CNOs/SWCNTs/GNPs) work best to fabricate IgA based electrochemical immunosensor. Methods: Different CNMs (CNOs, SWCNTs, GNPs) were tested for high electric current on GCE using square wave voltammetry (SWV), and among them, GNPs modified GCE platform (GNPs/GCE) showcased the highest electric current. Therefore, GNPs/GCE was utilized for the development of highly sensitive label-free electrochemical immunosensor for the detection of Immunoglobulin A using SWV. Results: Despite the simple fabrication strategies employed, the fabricated sensor demonstrated a low limit of detection of 50 fg mL-1 with an extensive linear range of detection from 50 fg mL-1 to 0.1 μg mL-1. Conclusion: Fabricated immunosensor represented high stability, repeatability, specificity and resistance to most common interferences as well as great potential to analyse the real sample.

Target-induced structure switching of DNA for label-free and ultrasensitive electrochemiluminescent detection of proteins

2014 ◽  
Vol 50 (24) ◽  
pp. 3211-3213 ◽  
Author(s):  
Mengli Yang ◽  
Ying Chen ◽  
Yun Xiang ◽  
Ruo Yuan ◽  
Yaqin Chai
Keyword(s):  
Dna Structure ◽  
Label Free ◽  
Switching Strategy ◽  
Highly Sensitive ◽  
Label Free Detection ◽  
Exo Iii ◽  
Recycling Amplification ◽  
Structure Switching ◽  
Induced Structure

Highly sensitive and label-free detection of thrombin is achieved via a target-induced DNA structure switching strategy and Exo III-assisted recycling amplification.

A highly sensitive fluorescence biosensor for detection of Staphylococcus aureus based on HCR-mediated three-way DNA junction nicking enzyme assisted signal amplification

The Analyst ◽  
2021 ◽  
Author(s):  
Chuyan Zhang ◽  
Zewei Luo ◽  
Mengfan Wu ◽  
Wei Ning ◽  
Ziyi Tian ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Food Safety ◽  
Signal Amplification ◽  
Sensitive Detection ◽  
Safety Control ◽  
Food Borne ◽  
Highly Sensitive ◽  
Fluorescence Biosensor ◽  
Highly Sensitive Detection ◽  
Nicking Enzyme

Sensitive and efficient monitoring of food-borne bacteria is of great importance for food safety control. Herein, a novel biosensor for highly sensitive detection of Staphylococcus aureus (S. aureus) was constructed...

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