scholarly journals Detection of Senecionine in Dietary Sources by Single-Use Electrochemical Sensor

Micromachines ◽  
2021 ◽  
Vol 12 (12) ◽  
pp. 1585
Author(s):  
Huseyin Senturk ◽  
Ece Eksin ◽  
Ulvi Zeybek ◽  
Arzum Erdem
Keyword(s):  
Secondary Compounds ◽  
Differential Pulse ◽  
Food Sources ◽  
Pencil Graphite Electrode ◽  
Electrochemical Assay ◽  
Natural Toxins ◽  
Contaminated Food ◽  
Passive Adsorption ◽  
Single Use ◽  
Toxic Plant

Pyrrolizidine alkaloids (PAs) are produced by plants as secondary compounds that are the most widely distributed natural toxins. There have been many cases of human toxicity caused by consumption of toxic plant species, as herbal teas and grain or grain products contaminated with PA-containing seeds have been reported. Companies that produce dried spices and tea leaves should examine the PA level in their products. For the first time in the literature, a simple and inexpensive electrochemical assay based on a single-use sensor was introduced for quantitative determination of senecionine (SEN) in the most frequently contaminated food sources. SEN was immobilized on a pencil graphite electrode surface by the passive adsorption technique. Differential pulse voltammetry (DPV) was used to evaluate the oxidation signal of SEN, which was observed to be around +0.95 V. The oxidation signal was specific to the SEN in the sample, and the current value was proportional to its concentration. The selectivity of our assay was also tested in the presence of other similar PAs such as intermedine, lycopsamine, and heliotrine. The detection limit is calculated by developed assay and found to be 5.45 µg/mL, which is an acceptable concentration value of SEN occurring at toxic levels for consumers. As an application of the developed sensor in food products, the electrochemical detection of SEN was successfully performed in flour and herbal tea products.

scholarly journals Contamination of food with cadmium and dioxins – influence on the human body

2019 ◽  
Vol 65 (3) ◽  
Author(s):  
Aleksandra Słojewska ◽  
Izabela Gutowska
Keyword(s):  
General Population ◽  
Health Effects ◽  
Human Body ◽  
Bone Mineralization ◽  
The Body ◽  
Food Sources ◽  
Hormonal Balance ◽  
Small Doses ◽  
Contaminated Food

Introduction: Food is a carrier of many pollutants nowadays. For cadmium and dioxins, it is the main source of these compounds in the general population. Hence, the aim of this work was to collect data on the health effects associated with exposure to cadmium and dioxins which get into the human body through food.Materials and methods: A literature review was conducted by searching the scientific Medline database (PubMed) and Google Scholar.Results: Health effects associated with a long-term exposure to low doses of cadmium and dioxins may be nonspecific and are rarely attributed to food sources. These may cause disorders affecting hormonal balance, kidney function, lipid metabolism, fertility, cognitive function, bone mineralization, and susceptibility to diseases and allergies. These compounds may also lead to cancer development.Conclusions: The general population faces the problem of longterm exposure to small doses of these substances due to their accumulation in the body, even if their amounts do not exceed the relevant standards. The health effects of this type of exposure may be significantly delayed and are not likely to be attributed to the consumption of contaminated food. Consumers wishing to reduce the amounts of these compounds delivered to the body should limit the consumption of grilled and smoked products, and should also opt for organic food.

scholarly journals Electrochemical Nanocomposite Single-Use Sensor for Dopamine Detection

Sensors ◽  
2019 ◽  
Vol 19 (14) ◽  
pp. 3097 ◽  
Author(s):  
Giulia Selvolini ◽  
Cinzia Lazzarini ◽  
Giovanna Marrazza
Keyword(s):  
Limit Of Detection ◽  
Differential Pulse ◽  
Sensor Response ◽  
Sensor Surface ◽  
Serum Samples ◽  
Dopamine Detection ◽  
Sensitive Sensor ◽  
Single Use ◽  
Pulse Voltammetry ◽  
Nanocomposite Sensor

In this work, we report the development of a simple and sensitive sensor based on graphite screen-printed electrodes (GSPEs) modified by a nanocomposite film for dopamine (DA) detection. The sensor was realized by electrodepositing polyaniline (PANI) and gold nanoparticles (AuNPs) onto the graphite working electrode. The sensor surface was fully characterized by means of the cyclic voltammetry (CV) technique using [Fe(CN)6]4−/3− and [Ru(NH3)6]2+/3+ as redox probes. The electrochemical behavior of the nanocomposite sensor towards DA oxidation was assessed by differential pulse voltammetry (DPV) in phosphate buffer saline at physiological pH. The sensor response was found to be linearly related to DA concentration in the range 1–100 μM DA, with a limit of detection of 0.86 μM. The performance of the sensor in terms of reproducibility and selectivity was also studied. Finally, the sensor was successfully applied for a preliminary DA determination in human serum samples.

scholarly journals Platinum(II) Complexes with Tetradentate Schiff Bases as Ligands: Synthesis, Characterization and Detection of DNA Interaction by Differential Pulse Voltammetry

2012 ◽  
Vol 9 (3) ◽  
pp. 1422-1430
Author(s):  
Lijun Li ◽  
Chao Tian ◽  
Cheng Wang ◽  
Guangyuan Wang ◽  
Lianzeng Wang ◽  
...  
Keyword(s):  
Differential Pulse Voltammetry ◽  
Schiff Bases ◽  
Differential Pulse ◽  
Dna Interaction ◽  
Resistance Mechanisms ◽  
Pencil Graphite Electrode ◽  
Drug Candidates ◽  
Fish Sperm ◽  
Guanine And Adenine ◽  
Pulse Voltammetry

Five sterically hindered platinum(II) complexes with tetradentate schiff bases as ligands, [Pt(L)] (L=N,N′-bisalicylidene-1,2-ethylenediamine (L1),N,N′-bisalicylidene-1,2-cyclohexanediamine (L2), N,N′-bis(5-hydroxyl-salicylidene)-1,2-cyclohexanediamine (L3),N,N′-bisalicylidene-1,2-diphenyl-ethylenediamine (L4) andN,N′-bis(3-tert-butyl-5-methyl-salicylidene)-1,2-diphenylethylenediamine (L5) ) have been synthesized and characterized by IR spectroscopy and elemental analysis. The sterical hindrance of antitumor drug candidates potentially makes them less susceptible to deactivation by sulphur containing proteins and helping to overcome resistance mechanisms. The interaction of these metal complexes with fish sperm single-stranded DNA (ssDNA) was studied electrochemically based on the oxidation signals of guanine and adenine. Differential pulse voltammetry was employed to monitor the DNA interaction in solution by using renewable pencil graphite electrode. The results indicate that ligands with different groups can strongly affect the interaction between [Pt(L)] complexes and ssDNA due to sterical hindrances and complex [Pt(L1)] has the best interaction with DNA among the five complexes.

scholarly journals Sonochemically Prepared GdWNFs/CNFs Nanocomposite As An Electrode Material For The Electrochemical Detection of Antibiotic Drug in Water Bodies

2021 ◽  
Author(s):  
Manikanta P ◽  
Hari Prasad B R ◽  
Sanjay B P ◽  
Sandeep S ◽  
Santhosh A S ◽  
...  
Keyword(s):  
Electrochemical Impedance ◽  
Differential Pulse ◽  
Electrochemical Sensing ◽  
Pencil Graphite Electrode ◽  
X Ray Diffraction ◽  
Standard Samples ◽  
Tem Analysis ◽  
X Ray ◽  
Transmission Electron ◽  
Antibiotic Drug

Abstract The work demonstrates the development of an electrochemical sensor for quantification of Chloramphenicol (CA) using pencil graphite electrode (PGE) modified with Gadolinium tungstate nano flakes and carbon nano fibers composite (PGE/GWNfs/CNFs). The composite was further characterized and confirmed by X-ray diffraction (XRD), energy-dispersive X-ray spectroscopy (EDS), Transmission electron microscopy (TEM) analysis. The prepared GWNfs/CNFs nano composite was fabricated by drop casting method to get PGE/GWNfs/CNFs working electrode. The modified electrode is then analyzed by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV) methods for its electrochemical and electrocatalytic property. The electrochemical investigation of developed sensor shows enhanced activity towards electro-oxidation of CA. The DPV studies revealed high efficacy characteristics such as sensitivity in the range 0.03984 µA µM-1cm-2, selectivity, good linear range (5-50 μM), and low detection limit (0.4 μM). The study benchmarks the use of GWNfs/CNFs as an excellent transducer material in electrochemical sensing of CA in standard samples thus, it finds an efficient potential application in the analysis of CA in environment sample analysis.

scholarly journals Chemical entrapment and killing of insects by bacteria

2020 ◽  
Author(s):  
Louis Ho ◽  
Martin Daniel-Ivad ◽  
Swathi Jeedigunta ◽  
Jing Li ◽  
Konstantin Iliadi ◽  
...  
Keyword(s):  
Significant Risk ◽  
Fruit Fly ◽  
Rapid Onset ◽  
Egg Laying ◽  
Food Sources ◽  
Whole Body ◽  
Specialized Metabolites ◽  
Contaminated Food ◽  
Antibacterial And Antifungal ◽  
Volatile Terpene

Abstract Actinobacteria such as the filamentous streptomycetes are widely known for their ability to produce specialized metabolites that include antibacterial and antifungal compounds. In addition, a growing body of work demonstrates that many insects harbour actinobacteria on their bodies and in their nests. The result of these mutualistic relationships is the protection of their offspring or food sources by virtue of the bacterially encoded specialized metabolites. However, some actinobacteria produce molecules that are toxic to insects and the relevance of this toxicity in nature is unknown. We have explored interactions between streptomycetes and the fruit fly Drosophila. We find that many streptomycetes produce specialized metabolites that have potent larvicidal effects against the fly. Larvae that ingest spores of the species that produce these toxic molecules die as a result. Strikingly, the mechanism of toxicity is specific to the bacterium’s chemical arsenal: cosmomycin D producing cells induce a relatively slow-acting cell death-like response in the larval digestive tract and avermectin producing cells induce rapid onset, whole-body paralysis. We further show that fruit flies are attracted to the volatile terpene 2-methylisoborneol that is produced by most streptomycetes. This interaction can influence their food choice and egg-laying destination such that they preferentially deposit their eggs on contaminated food sources. As a result, the larvae that hatch in these toxic environments are subsequently killed. This phenomena of terpene-mediated attraction and specialized metabolite toxicity must pose a significant risk to insects in nature.

scholarly journals A “Single-Use” Ceramic-Based Electrochemical Sensor Chip Using Molecularly Imprinted Carbon Paste Electrode

Sensors ◽  
2020 ◽  
Vol 20 (20) ◽  
pp. 5847
Author(s):  
Aaryashree ◽  
Yuuto Takeda ◽  
Momoe Kanai ◽  
Akihiko Hatano ◽  
Yasuo Yoshimi ◽  
...  
Keyword(s):  
Differential Pulse ◽  
Sensor Chip ◽  
Therapeutic Drug ◽  
Carbon Paste ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Single Use ◽  
Mip Sensor ◽  
Response Current ◽  
Time Required

An inexpensive disposable electrochemical drug sensor for the detection of drugs (vancomycin, meropenem, theophylline, and phenobarbital) is described. Molecularly imprinted polymer (MIP) templated with the target drugs was immobilized on the surface of graphite particles using a simple radical polymerization method and packed into the working electrode of a three-electrode ceramic-based chip sensor. Differential pulse voltammetry (DPV) was used to determine the relationship between the response current and the concentration of the targeted drug while using one sensor chip for one single operation. The time required for each DPV measurement was less than 2 min. Concentrations corresponding to the therapeutic range of these drugs in plasma were taken into account while performing DPV. In all the cases, the single-used MIP sensor showed higher sensitivity and linearity than non-imprinted polymer. The selectivity test in drugs with a structure similar to that of the target drugs was performed, and it was found that MIP-based sensors were more selective than the untreated ones. Additionally, the test in whole blood showed that the presence of interfering species had an insignificant effect on the diagnostic responses of the sensor. These results demonstrate that the disposable MIP-sensor is promising for quick and straightforward therapeutic drug monitoring to prevent the toxic side effects and the insufficient therapeutic effect due to the overdose and underdose, respectively.

Sign in / Sign up

Export Citation Format

Share Document