scholarly journals Unraveling the Photocatalytic Mechanisms on TiO2 Surfaces Using the Oxygen-18 Isotopic Label Technique

Molecules ◽  
2014 ◽  
Vol 19 (10) ◽  
pp. 16291-16311 ◽  
Author(s):  
Xibin Pang ◽  
Chuncheng Chen ◽  
Hongwei Ji ◽  
Yanke Che ◽  
Wanhong Ma ◽  
...  
Keyword(s):  
Label Technique ◽  
Isotopic Label

scholarly journals pH Behavior of Polymer Complexes between Poly(carboxylic acids) and Poly(acrylamide derivatives) Using a Fluorescence Label Technique

Polymers ◽  
2019 ◽  
Vol 11 (7) ◽  
pp. 1196 ◽  
Author(s):  
Yuriko Matsumura ◽  
Kaoru Iwai
Keyword(s):  
Hydrogen Bond ◽  
Carboxylic Acids ◽  
Interpolymer Complex ◽  
Interpolymer Complexes ◽  
Hydrogen Bond Formation ◽  
Label Technique ◽  
Fluorescence Label ◽  
Subsequent Decrease ◽  
Mixed Systems ◽  
Poly Acrylamide

In order to clarify the local environment during interpolymer complex formation between poly(carboxylic acids) and poly(acrylamide derivatives) with different N-substitutions, a fluorescence label technique was used. 3-(2-propenyl)-9-(4-N,N-dimethylaminophenyl) phenanthrene (VDP) was used as an intramolecular fluorescence probe. All polymers were synthesized by free radical polymerization. Interpolymer complexation was monitored by charge transfer emission from the VDP unit. Both of the poly(carboxylic acids) formed interpolymer complexes with poly(N,N-dimethylacrylamide) (polyDMAM). The micro-environments around the VDP unit in the acidic pH region for the poly(methacrylic acid) (polyMAAc) and polyDMAM mixed systems were more hydrophobic than those of the poly(acrylic acid) (polyAAc) and polyDMAM mixed systems, as the α-methyl group of the MAAc unit contributed to hydrophobicity around the polymer chain during hydrogen bond formation. This suggests that, when the poly(carboxylic acids) and poly(acrylamide derivatives) were mixed, with a subsequent decrease in the solution pH, a hydrogen bond was partially formed, following which the hydrophobicity of the micro-environment around the polymer chains was changed, resulting in the formation of interpolymer complexes. Moreover, the electron-donating ability of the carbonyl group in the poly(acrylamide derivatives) had an effect on complexation with poly(carboxylic acids).

scholarly journals The uptake of 3H-labelled monodeoxyfluoro-myo-inositols into thymocytes and their incorporation into phospholipid in permeabilized cells

1993 ◽  
Vol 291 (2) ◽  
pp. 553-560 ◽  
Author(s):  
J Offer ◽  
J C Metcalfe ◽  
G A Smith
Keyword(s):  
Cellular Responses ◽  
Effective Inhibitor ◽  
Permeabilized Cells ◽  
Label Technique ◽  
Competitive Inhibitors ◽  
Phosphatidylinositol Synthase ◽  
Potential Inhibitors ◽  
Dual Label ◽  
Inositol Uptake

Monodeoxyfluoro-myo-inositols were applied to electropermeabilized and intact thymocyte preparations to study their metabolism and uptake in order to investigate their suitability as potential inhibitors of phosphoinositide-mediated cellular responses. Only three of the monodeoxyfluoro-myo-inositols were incorporated into the phospholipids of thymocytes: 1D-3-deoxy-3-fluoro-myo-inositol, 5-deoxy-5-fluoro-myo-inositol and 1D-6-deoxy-6-fluoro-myo-inositol, all of which were weaker substrates for phosphatidylinositol synthase than was myo-inositol. The 3-, 5- and 6-fluoro analogues also behaved as competitive inhibitors, with K1 values of 350 +/- 5 microM, 350 +/- 5 microM and 2.9 +/- 2 mM respectively, compared with a Km for myo-inositol of 31 +/- 4 microM. When incubated with electropermeabilized thymocyte preparations, these three analogues of myo-inositol all formed phospholipids with chromatographic properties which corresponded to those of substituted phosphatidylinositol and phosphatidylinositol monophosphate. The uptake of myo-inositol and of the monodeoxyfluoro-myo-inositols into intact thymocytes was studied by a dual-label technique. All the monodeoxyfluoro-myo-inositols were taken up to some extent, but only 2-deoxy-2-fluoro-myo-inositol and 1D-3-deoxy-3-fluoro-myo-inositol were actively concentrated. The monodeoxyfluoro-myo-inositols were also assayed for their ability to inhibit the uptake of myo-inositol into cells. Both 2-deoxy-2-fluoro-myo-inositol and 1D-3-deoxy-3-fluoro-myo-inositol were effective inhibitors of myo-inositol uptake. Furthermore, 1D-1-deoxy-1-fluoro-myo-inositol, which was not taken up actively, was an effective inhibitor of myo-inositol uptake. The three effective inhibitors all showed Ki values of approximately 150 microM, close to the apparent Km for inositol uptake of 180 microM, and the 4-, 5- and 6-fluoro analogues had Ki values in excess of 10 mM.

A new dual-label technique for platelet survival studies with the use of111indium and114mindium tropolonate

1991 ◽  
Vol 78 (2) ◽  
pp. 236-241 ◽  
Author(s):  
R. A. de Vries ◽  
M. de Bruin ◽  
S. J. Oldenburg ◽  
A. Zwiers ◽  
J. J. M. Marx ◽  
...  
Keyword(s):  
Label Technique ◽  
Platelet Survival ◽  
Survival Studies ◽  
Dual Label

scholarly journals Relevance of pretransfusion incubation of platelets at 37 degrees C

Blood ◽  
1992 ◽  
Vol 80 (6) ◽  
pp. 1599-1602
Author(s):  
RA de Vries ◽  
JG Gerritsen ◽  
M de Bruin ◽  
JJ Marx ◽  
HC Hart ◽  
...  
Keyword(s):  
Beneficial Effect ◽  
Mean Platelet Volume ◽  
Healthy Subjects ◽  
The Other ◽  
Platelet Concentrates ◽  
Platelet Volume ◽  
Label Technique ◽  
The Mean ◽  
Initial Recovery ◽  
Dual Label

The effect of pretransfusion incubation of platelets at 37 degrees C was assessed because of the controversial reports about its relevance. A dual-label technique (111indium and 114mindium) was applied in 10 healthy subjects receiving warmed and unwarmed autologous platelets simultaneously. Fresh platelet concentrates were infused into five subjects, whereas the other five subjects received stored platelet concentrates. The mean platelet volume decreased in all platelet concentrates during incubation, reflecting the restoration of the discoid shape of the platelets. The mean decrease was 0.35 fL (P = .003). However, the initial recovery and the mean platelet life-span were not improved by this procedure. It was concluded that there is no evidence that brief warming of platelets has any beneficial effect on platelet viability in healthy volunteers.

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