scholarly journals E-Configuration Improves Antioxidant and Cytoprotective Capacities of Resveratrols

Molecules ◽  
2018 ◽  
Vol 23 (7) ◽  
pp. 1790 ◽  
Author(s):  
Jian Lin ◽  
Xican Li ◽  
Ban Chen ◽  
Gang Wei ◽  
Dongfeng Chen
Keyword(s):  
Bone Marrow ◽  
Detrimental Effect ◽  
Hydrogen Atom Transfer ◽  
Dihedral Angles ◽  
Antioxidant Action ◽  
Antioxidant Power ◽  
Cellular Assays ◽  
Ferric Reducing Antioxidant Power ◽  
Quantitative Analyses ◽  
Dpph Scavenging

The antioxidant and cytoprotective capacities of E-resveratrol and Z-resveratrol were compared using chemical and cellular assays. Chemical assays revealed that the two isomers were dose-dependently active in •O2−-scavenging, ferric reducing antioxidant power (FRAP), Cu2+-reducing antioxidant capacity (CUPRAC), 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl 3-oxide radical (PTIO•)-scavenging (pH 7.4 and pH 4.5), and 1,1-diphenyl-2-picryl-hydrazyl (DPPH•)-scavenging assays. The cellular assay indicated that the two isomers could also increase cell viabilities. However, quantitative analyses suggested that E-resveratrol exhibited stronger effects than Z-resveratrol in all chemical and cellular assays. Finally, the conformations of E-resveratrol and Z-resveratrol were analyzed. It can be concluded that both E-resveratrol and Z-resveratrol can promote redox-related pathways to exhibit antioxidant action and consequently protect bone marrow-derived mesenchymal stem cells (bmMSCs) from oxidative damage. These pathways include electron transfer (ET) and H+-transfer, and likely include hydrogen atom transfer (HAT). The E-configuration, however, improves antioxidant and cytoprotective capacities of resveratrols. The detrimental effect of the Z-configuration may be attributed to the non-planar preferential conformation, where two dihedral angles block the extension of the conjugative system.

scholarly journals Total Polyphenol and Flavonoid Content and Antioxidant Capacity of Some Varieties of Persea americana Peels Consumed in Cameroon

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Ruth Edwige Kemadjou Dibacto ◽  
Boris Ronald Tonou Tchuente ◽  
Maxwell Wandji Nguedjo ◽  
Yves Martial Tongue Tientcheu ◽  
Emilienne Carine Nyobe ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Aqueous Extract ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Persea Americana ◽  
Total Phenolic ◽  
Total Polyphenol ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power ◽  
Dpph Scavenging

Fruit peels are increasingly being used as functional foods nowadays. Peelings of twelve varieties of Persea americana fruits consumed in Cameroon were investigated for their phenolic compounds (polyphenols and flavonoids) using three solvents systems, water, ethanol: water (50 : 50 v / v ), and ethanol, and antioxidant activity using total antioxidant capacity (TAC), ferric reducing antioxidant power (FRAP), and 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging methods. Total polyphenol, flavonoids, and antioxidant potential of the peels significantly varied with P. americana variety and also with the extraction solvents in the order ethanol > ethanol: water > water. Total phenolic content varied from 2407 (Fuerte florid) to 673 (Semil) mg GAE/g DM, respectively, while flavonoids varied from 986 to 119 mg QE/g DM for Fuerte florid and Hickson varieties, respectively. TAC, respectively, varied between 132.87 and 126.85 mg AAE/g DM with Hass and Semil varieties, respectively. The highest DPPH scavenging capacity was recorded for the ethanolic extract with Lula (86.33%) and the least for the aqueous extract with the Semil (30.11%) variety. With FRAP, the highest capacity was obtained with hydroethanolic extract of Fuerte florid (0.43 mg AAE/g DM) and the least for aqueous extract with the Semil (0.269 mg AAE/g DM) variety. In conclusion, varieties of avocado peels are a good source of antioxidants. Solvent extraction significantly affected the concentration of bioactive compounds but not the potency of the antioxidants. A weakly positive correlation but not significant between the quantity of polyphenol, flavonoid, and antioxidant capacity of avocado peelings was obtained in this study.

scholarly journals Physiological responses of resistant and susceptible pepper plants to exogenous proline application under Phytophthora capsici stress

2022 ◽  
Vol 81 (1) ◽  
Author(s):  
Esra Koç
Keyword(s):  
Chlorophyll A ◽  
Phytophthora Capsici ◽  
Soluble Carbohydrates ◽  
Chlorophyll B ◽  
Antioxidant Power ◽  
The Third ◽  
Ferric Reducing Antioxidant Power ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging ◽  
Positive Effect

Phytophthora capsici Leon. is the main pathogen that limits the production of peppers. In this study, the effects of 1 and 10 mM proline (Pro), prior to exposure of resistant (CM-334) and susceptible (SD-8) pepper seedlings to P. capsici, on some physiological parameters were investigated. A lower Pro concentration (1 mM) was found to be more effective than 10 mM Pro in increasing the stress tolerance of the CM-334 cultivar. Namely, in CM-334 cultivar, the highest chlorophyll a, chlorophyll b, carotenoid, glucose and fructose content and 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity percentage were detected on the seventh day after application of 1 mM Pro + P. capsici, while the lowest malondialdehyde (MDA) amount was measured on the third day in the same treatment. The highest ferric reducing antioxidant power (FRAP) increase was determined on the seventh day in the 10 mM Pro + P. capsici application. The effects of the same Pro treatments on the SD-8 cultivar somewhat differed; the highest amounts of chlorophyll a, chlorophyll b, anthocyanins, fructose, total protein and endogenous Pro were detected on the seventh day in the 1 mM Pro + P. capsici application, while the lowest MDA amount was measured on the third day after the 10 mM Pro + P. capsici application, the highest DPPH % and FRAP values were detected on the seventh day with 10 mM Pro + P. capsici application. Although some differences were detected between the cultivars, Pro application against the P. capsici stress in general resulted in a positive effect on photosynthetic pigments, soluble carbohydrates and antioxidant capacity in pepper. The exogenous application of Pro helped the non-resistant cultivar to overcome the stress.

scholarly journals Chemical composition and antioxidant activity of three species of Cornukaempferia in Thailand

2021 ◽  
Vol 22 (9) ◽  
Author(s):  
THEERAPHAN CHUMROENPHAT ◽  
Surapon Saensouk ◽  
PIYAPORN SAENSOUK
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Antioxidant Activities ◽  
Dry Weight ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Plant Parts ◽  
Ferric Reducing Antioxidant Power ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

Abstract. Chumroenphat T, Saensouk S, Saensouk P. 2021. Chemical composition and antioxidant activity of three species of Cornukaempferia in Thailand. Biodiversitas 22: 4036-4044. Genus Cornukaempferia (Zingiberaceae) were used as medicinal plants in Thailand. Cornukaempferia has never been reported the chemical compounds and antioxidant activity before. This study was designed to identify the chemical composition and antioxidant activities in root, rhizome, stem, and leaves of Cornukaempferia aurantiflora, C. larsenii, and C. longipetiolata. DPPH scavenging activity and Ferric reducing antioxidant power (FRAP) assays assessed the chemical composition and antioxidant activity. The results showed that total phenolic content (TPC) and total flavonoid content (TFC) found different plant parts obtained in this study were in the order of root>rhizomes>leaves>?stems. The C. aurantiflora had a high TPC of 416.39 mg GAE/g dry weight, while individual phenolics, such as vanillic acid at 256.90 mg/g dry weight, showed high contents in the leaf part. For C. larsenii,  TFC and individual flavonoids, such as catechin, have high 62.52 and 56.47 mg/100g dry weight values in the roots, respectively. The antioxidant activities in different species of different plant parts (DPPH: root>leaves?> rhizomes?>?stems; FRAP: leaves?>root>?rhizomes?>?stems). The genus Cornukaempferia is a rich source of bioactive compounds and antioxidant activity. Therefore, the genus Cornukaempferia will be used as medicine and food to produce products with health benefits in the future.

scholarly journals In VitroStudy on the Antioxidant Potentials of the Leaves and Fruits ofNauclea latifolia

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Ademola O. Ayeleso ◽  
Oluwafemi O. Oguntibeju ◽  
Nicole L. Brooks
Keyword(s):  
Aqueous Extract ◽  
Antioxidant Activities ◽  
Hot Water ◽  
Total Polyphenols ◽  
Total Polyphenol ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power ◽  
Dpph Scavenging ◽  
Nauclea Latifolia

This study was carried out to investigate thein vitroantioxidant potentials of the leaves and fruits ofNauclea latifolia, a straggling shrub or small tree, native to tropical Africa and Asia. Hot water extracts of the leaves and fruits ofNauclea latifoliawere assessed for their total polyphenolic, flavanol, and flavonol contents as well as 1-diphenyl-2-picrylhydrazyl (DPPH) scavenging ability, ferric reducing antioxidant power (FRAP), Trolox equivalence antioxidant capacity (TEAC), and oxygen radical absorbance capacity (ORAC) assays. The aqueous extract of the leaves was found to contain higher level of total polyphenols (11.63±0.023 mg GAE/g), flavanol (1.45±0.10 mg CE/g), and flavonol (2.22±0.37 mg QE/g) than the extract of the fruits with values of1.75±0.02 mg GAE/g (total polyphenol),0.15±0.01 mg CE/g (flavanol), and1.00±0.13 mg QE/g (flavonol). Similarly, the aqueous extract of the leaves also exhibited higher DPPH (IC5020.64 mg/mL), FRAP (86.10±3.46 μmol AAE/g), TEAC (94.83±3.57 μmol TE/g), and ORAC (196.55±0.073 μmol TE/g) than the extract of the fruits with DPPH (IC50120.33 mg/mL), FRAP (12.23±0.40 μmol AAE/g), TEAC (12.48±0.21 μmol TE/g), and ORAC (58.88±0.073 μmol TE/g). The present study showed thatNauclea latifoliahas strong antioxidant potentials with the leaves demonstrating higherin vitroantioxidant activities than the fruits.

scholarly journals EVALUATION OF IN VITRO- ANTI-OXIDANT POTENTIAL OF AQUEOUS ROOT EXTRACT OF CLERODENDRUM SERRATUM L.

2017 ◽  
Vol 10 (4) ◽  
pp. 402
Author(s):  
Raj Kumar Tiwari ◽  
Udayabanu Malairaman ◽  
Silpi Chanda
Keyword(s):  
Radical Scavenging ◽  
Reducing Power ◽  
Antioxidant Property ◽  
Root Extract ◽  
Antioxidant Power ◽  
Ic50 Values ◽  
Ferric Reducing Antioxidant Power ◽  
Dpph Scavenging ◽  
Aqueous Root Extract

Objective: The intent  of this report  was to investigate the effect of aqueous root extract of Clerodendrum serratum L. for antioxidant activity using divergent models viz. DPPH scavenging assay, Superoxide scavenging assay and Ferric Reducing Antioxidant Power (FRAP) assay.Materials and Methods: The root of C. serratum was extracted using water. The yield of aqueous extract was 10%w/w. The outcome was examined statistically by the regression method.Results and discussions: The IC50 values are 85.43 µg/ml and 107.59 µg/ml for DPPH radical scavenging and Superoxide scavenging assay respectively whereas  FRAP showed significant reducing power activity with increased concentration of sample. The pilot study showed, a significant correlation existed between concentrations of the extract and percentage engrossment of free radicals.Conclusion: The antioxidant property may be corresponding to the polyphenols and flavonoids adjacent in the extract. These results clearly revealed that C. serratum might be effective against diseases analogous with free radical mediated. Keywords Clerodendrum serratum, DPPH, Superoxide, FRAP, Rutin, Antioxidant

scholarly journals Antioxidants Distribution in Pulp and Seeds of Black and Red Goji Berries as Affected by Boiling Processing

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Guang Xin ◽  
Fengmei Zhu ◽  
Bin Du ◽  
Baojun Xu
Keyword(s):  
Antioxidant Activities ◽  
Condensed Tannin ◽  
Total Phenolic ◽  
Anthocyanin Content ◽  
Antioxidant Power ◽  
Phytochemical Content ◽  
Scavenging Capacity ◽  
Ferric Reducing Antioxidant Power ◽  
Goji Berries ◽  
Dpph Scavenging

Pulp and seeds from four varieties of black goji and two varieties of red goji with different geographical origins were examined for their total phenolic content (TPC), total flavonoid content (TFC), condensed tannin content (CTT), monomeric anthocyanin content (MAC), 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging capacity, and ferric reducing antioxidant power (FRAP) using colorimetric methods. Contribution rates of pulp and seed to phytochemical contents and overall antioxidant capacities of whole fruits were calculated for each parameter. It was observed that most of the phytochemicals and antioxidant activities were predominantly contributed by the pulp in all six varieties. Boiling led to significant (p< 0.05) losses in the phytochemical content and antioxidant capacity. The average MAC value in black goji was significantly (p< 0.05) higher than that observed for red goji.

scholarly journals Phytochemical Investigation and Biological Activities of Lantana rhodesiensis

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 846
Author(s):  
Fatimata Nea ◽  
Michel Boni Bitchi ◽  
Manon Genva ◽  
Allison Ledoux ◽  
Alembert Tiabou Tchinda ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Leaf Extract ◽  
Biological Activities ◽  
Phytochemical Study ◽  
Antioxidant Power ◽  
Root Extracts ◽  
Bleaching Test ◽  
Positive Linear Correlation ◽  
Ferric Reducing Antioxidant Power ◽  
Dpph Scavenging

Lantana rhodesiensis Moldenke is a plant widely used to treat diseases, such as rheumatism, diabetes, and malaria in traditional medicine. To better understand the traditional uses of this plant, a phytochemical study was undertaken, revealing a higher proportion of polyphenols, including flavonoids in L. rhodesiensis leaf extract and moderate proportion in stem and root extracts. The antioxidant activity of the extracts was also determined using three different assays: the radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity, the FRAP method (Ferric-reducing antioxidant power) and the β-carotene bleaching test. The anti-malarial activity of each extract was also evaluated using asexual erythrocyte stages of Plasmodium falciparum, chloroquine-sensitive strain 3D7. The results showed that the leaf extract exhibited higher antioxidant and anti-malarial activities in comparison with the stem and root extracts, probably due to the presence of higher quantities of polyphenols including flavonoids in the leaves. A positive linear correlation was established between the phenolic compound content (total polyphenols including flavonoids and tannins; and total flavonoids) and the antioxidant activity of all extracts. Furthermore, four flavones were isolated from leaf dichloromethane and ethyl acetate fractions: a new flavone named rhodescine (5,6,3′,5′-tetrahydroxy-7,4′-dimethoxyflavone) (1), 5-hydroxy-6,7,3′,4′,5′-pentamethoxyflavone (2), 5-hydroxy-6,7,3′,4′-tetramethoxyflavone (3), and 5,6,3′-trihydroxy-7,4′-dimethoxyflavone (4). Their structures were elucidated by 1H, 13CNMR, COSY, HSQC, HMBC, and MS-EI spectral methods. Aside from compound 2, all other molecules were described for the first time in this plant species.

scholarly journals UHPLC–Q/Orbitrap/MS/MS Fingerprinting, Free Radical Scavenging, and Antimicrobial Activity of Tessaria absinthiodes (Hook. & Arn.) DC. (Asteraceae) Lyophilized Decoction from Argentina and Chile

Antioxidants ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 593 ◽  
Author(s):  
Jessica Gómez ◽  
Mario J. Simirgiotis ◽  
Beatriz Lima ◽  
Carlos Gamarra-Luques ◽  
Jorge Bórquez ◽  
...  
Keyword(s):  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Antibacterial Activities ◽  
Antioxidant Power ◽  
Aerial Parts ◽  
Trolox Equivalent ◽  
Ferric Reducing Antioxidant Power ◽  
Dpph Scavenging ◽  
Orbitrap Ms

The decoction of Tessaria absinthioides is used in traditional medicine of South America as hypocholesterolemic, balsamic, and expectorant; but it is also useful for the prevention of hepatitis, renal insufficiency, and diabetes, and is used as digestive. A lyophilized decoction from the aerial parts of this plant (TLD) collected in San Juan (TLDSJ) and Mendoza (TLDM) provinces (Argentina) and one collection from Antofagasta, Chile (TLDCH) were characterized regarding antioxidant and antibacterial activities, phenolics and flavonoids content, and ultrahigh resolution liquid chromatography Orbitrap MS analysis UHPLC–PDA–OT-MS/MS metabolite profiling. The antioxidant properties were carried out "in vitro" using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and trolox equivalent antioxidant activity (TEAC) methods, ferric-reducing antioxidant power (FRAP), and lipoperoxidation in erythrocytes (LP). The antibacterial activity was evaluated following the Clinical and Laboratory Standards Institute (CLSI) rules. TLDSJ, TLDM, and TLDCH displayed a strong DPPH scavenging activity (EC50 = 42, 41.6, and 43 µg/mL, respectively) and inhibition of lipoperoxidation in erythrocytes (86–88% at 250 µg TLD/mL), while a less effect in the FRAP and TEACantioxidant assays was found. Additionally, the decoctions showed a content of phenolics compounds of 94 mg gallic acid equivalents (GAE)/g, 185 GAE/g, and 64 GAE/g, for TLDSJ, TLDM, and TLDCH samples, respectively. Regarding the flavonoid content, the Chilean sample was highlighted with 19 mg quercetin equivalents (QE)/g. In this work, several phenolic compounds, including sesquiterpenes, flavonoids, and phenolic acids, were rapidly identified in TLDSJ, TLDM, and TLDCH extracts by means UHPLC–PDA–OT-MS/MS for the first time, which gave a first scientific support to consider this medicinal decoction from both countries as a valuable source of metabolites with antioxidant effects, some with outstanding potential to improve human health.

scholarly journals Preliminary Screening for Anti-Inflammatory, Anti-Nociceptive and Antioxidant Activities of Five New Synthesized Paratoluene Sulphonamide Derivatives of Amino Acids

2016 ◽  
Vol 5 ◽  
pp. 74-78
Author(s):  
Chinyere Benardette Chinaka Ikpa ◽  
U.C. Okoro ◽  
Collins I. Ubochi ◽  
Kieran O. Nwanorh ◽  
Samuel O. Onoja
Keyword(s):  
Amino Acids ◽  
Antioxidant Activities ◽  
Tail Flick ◽  
Antioxidant Power ◽  
Preliminary Screening ◽  
Ferric Reducing Antioxidant Power ◽  
Anti Inflammatory ◽  
Dpph Scavenging ◽  
Derivatives Of

The anti-inflammatory, antinociceptive and antioxidant activities of five new synthesized paratoluene sulphonamide derivatives of amino acids were evaluated in this study. The anti-inflammatory and antinociceptive was evaluated using egg albumin induced paw edema and tail flick rat model respectively, at the dose of 400 mg/kg; while the antioxidant activity was evaluatedin vitrousing 2, 2-Diphenyl-1-Picrylhydrazyl (DPPH) scavenging and Ferric Reducing Antioxidant Power assay. The compounds produced various degrees of anti-inflammatory and antinociceptive activities but did not produce antioxidant effect. The sulphonamide derivative has anti-inflammatory and antinociceptive properties and can be used as drug for this purpose.

scholarly journals An Assessment of Total Polyphenolic Content and Antioxidant Potential of Mauby Bark Extracts (Colubrina arborescens) Brewed for Different Lengths of Time

2019 ◽  
Vol 8 (3) ◽  
pp. 26
Author(s):  
Jeremy Embola ◽  
Cheryl Rock ◽  
Long Wang ◽  
Wendy Reiboldt ◽  
Saba Ahmed ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Antioxidant Power ◽  
Polyphenolic Content ◽  
Ferric Reducing Antioxidant Power ◽  
Dpph Scavenging Activity ◽  
Total Polyphenolic Content ◽  
The Caribbean ◽  
Dpph Scavenging

Mauby bark (Colubrina arborescens) is commonly used to make a beverage,&ldquo;Mauby&rdquo;, in the Caribbean and is believed to possess antiglycemic, antilipidemic, and anticarcinogenic properties. However, limited studies have been conducted to substantiate the compounds present that may confer these benefits. Therefore, the objectives of this research were to quantify the total polyphenolic content and evaluate the antioxidant capacity of Mauby bark extracts brewed in water at 30, 45, and 60 minutes. In the extracts, the Total Flavonoid Content (TFC) ranged from 1.93 - 3.17 mg CE/mL and the Total Phenolic Content (TPC) ranged from 2.10 mg &plusmn; 0.11 GAE/mL (45 minutes) - 2.36 mg &plusmn; 0.067 GAE/mL (30 minutes). Moreover, their antioxidant activity was assessed using the 2,2 Diphenyl 1-Picrylhydrazyl (DPPH) and&nbsp; Ferric Reducing Antioxidant Power (FRAP) assays. The DPPH scavenging activity observed from Mauby extracts ranged from 75% &plusmn; 4.02 (30 minutes) to 83% &plusmn; 0.66 (60 minutes) and the FRAP values ranged from 6.29&plusmn; 0.84 (30 minutes) to 6.90 &plusmn; 1.54 mM FeSO4 equivalents/ 0.2 mL Mauby extract (45 minutes). Although, polyphenolic content at 30 minutes was greater than 60 minutes of brewing for TFC (p &lt; 0.001) and TPC (p = 0.002), the scavenging activity was greater at 60 minutes than 30 minutes (p = 0.014) while antioxidant power was not affected by brewing time (p = 0.736). In summary, brewing the bark at 60 minutes was observed to provide the highest antioxidant activity.

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