scholarly journals Antiplasmodial and Cytotoxic Flavonoids from Pappea capensis (Eckl. & Zeyh.) Leaves

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 3875
Author(s):  
Nasir Tajuddeen ◽  
Tarryn Swart ◽  
Heinrich C. Hoppe ◽  
Fanie R. van Heerden
Keyword(s):  
Hela Cells ◽  
Leaf Extract ◽  
Uv Spectroscopy ◽  
Flavonoid Glycosides ◽  
Plant Leaves ◽  
Chromatographic Purification ◽  
Resazurin Assay ◽  
Adenocarcinoma Cells ◽  
Selectivity Studies ◽  
Human Cervix

Ethnobotanical surveys indicate that the Masai and Kikuyu in Kenya, the Venda in South Africa, and the Gumuz people of Ethiopia use Pappea capensis for the treatment of malaria. The present study aimed to investigate the phytochemical and antiplasmodial properties of the plant leaves. The bioactive compounds were isolated using chromatographic techniques. The structures were established using NMR, HRMS, and UV spectroscopy. Antiplasmodial activity of P. capensis leaf extract and isolated compounds against chloroquine-sensitive 3D7 P. falciparum was evaluated using the parasite lactate dehydrogenase assay. Cytotoxicity against HeLa (human cervix adenocarcinoma) cells was determined using the resazurin assay. The extract inhibited the viability of Plasmodium falciparum by more than 80% at 50 µg/mL, but it was also cytotoxic against HeLa cells at the same concentration. Chromatographic purification of the extract led to the isolation of four flavonoid glycosides and epicatechin. The compounds displayed a similar activity pattern with the extract against P. falciparum and HeLa cells. The results from this study suggest that the widespread use of P. capensis in traditional medicine for the treatment of malaria might have some merits. However, more selectivity studies are needed to determine whether the leaf extract is cytotoxic against noncancerous cells.

Effects of Papaya Leaf Extract (Carica papaya L.) on Cellular Proliferation and Apoptosis in Cervical Cancer Mice Model

2019 ◽  
Vol 17 (5) ◽  
pp. 265-275
Author(s):  
Y. Peristiowati ◽  
Y. Puspitasari ◽  
Indasah
Keyword(s):  
Cervical Cancer ◽  
Cell Proliferation ◽  
Hela Cells ◽  
Leaf Extract ◽  
Caspase 3 ◽  
Methanol Extract ◽  
Negative Control ◽  
Proliferation Index ◽  
Control Group ◽  
P53 Expression

This study is aimed at analyzing the anticancer properties of papaya leaf extract, specifically the inhibition of cell proliferation and apoptotic induction through nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and p53 pathways. Twenty-five mice (Mus musculus), aged 2 months and weighing 20–30 g, was injected with 0.5 mg dexamethasone for 7 days. The mice were then injected intracutaneously with 1 ml of HeLa cells (8 × 106 HeLa cells/microliter). The mice were divided into five groups (5 each): negative control (P1) (5% CMC-Na, sodium carboxymethyl cellulose), treatment II (225 mg/kg BW (body weight) papaya leaves methanol extract), treatment III (450 mg/kg BW), treatment IV (750 mg/kg BW), and treatment PV (2 mg alcohol anticancer drug). Papaya leaf extract treatments were applied for 2 weeks. Then, the tumor tissue was isolated for hematoxylin and eosin staining. Immunohistochemical imaging was used to detect Ki-67, caspase-3, NF-κB, and p53 expression. Further analysis was undertaken using the ImmunoRatio software program. The results indicated that administration of papaya leaf methanol extract significantly increased the expression of NF-κB and p53 at a dose of 450 mg/kg BW. Our results also showed that the mice treated with 450 mg of papaya leaf extract per kg of BW (P3) had the largest increase of caspase-3 expression compared to the negative control group. Papaya leaf ethanol extract decreased the cancer cell proliferation index and increased apoptosis of cancer cells in animal models of cervical cancer; it may also work to increase NF-kB expression and expression of the p53 gene.

Antiproliferative and apoptosis inducing activity of Markhamia tomentosa leaf extract on HeLa cells

2013 ◽  
Vol 149 (3) ◽  
pp. 745-749 ◽  
Author(s):  
Bolanle Ibrahim ◽  
Abimbola Sowemimo ◽  
Luanne Spies ◽  
Trevor Koekomoer ◽  
Maryna van de Venter ◽  
...  
Keyword(s):  
Hela Cells ◽  
Leaf Extract

High-efficiency transfer and expression of AdCMV-p53 in human cervix adenocarcinoma cells induced by subclinical-dose carbon beam radiation

2008 ◽  
Vol 135 (7) ◽  
pp. 925-932
Author(s):  
Bing Liu ◽  
Hong Zhang ◽  
Xiaohong Luo ◽  
Yi Xie ◽  
Jifang Hao ◽  
...  
Keyword(s):  
High Efficiency ◽  
Beam Radiation ◽  
Adenocarcinoma Cells ◽  
Carbon Beam ◽  
Human Cervix

scholarly journals Rapid Biosynthesis of Silver Nanoparticles Using Pepino (Solanum muricatum) Leaf Extract and Their Cytotoxicity on HeLa Cells

Materials ◽  
2016 ◽  
Vol 9 (5) ◽  
pp. 325 ◽  
Author(s):  
Mónica Gorbe ◽  
Ravishankar Bhat ◽  
Elena Aznar ◽  
Félix Sancenón ◽  
M. Marcos ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Hela Cells ◽  
Leaf Extract ◽  
Solanum Muricatum

Novel bismuth compounds: synthesis, characterization and biological activity against human adenocarcinoma cells

RSC Advances ◽  
2016 ◽  
Vol 6 (35) ◽  
pp. 29026-29044 ◽  
Author(s):  
M. Arda ◽  
I. I. Ozturk ◽  
C. N. Banti ◽  
N. Kourkoumelis ◽  
M. Manoli ◽  
...  
Keyword(s):  
Biological Activity ◽  
Molecular Interactions ◽  
Hela Cells ◽  
Bismuth Compounds ◽  
Adenocarcinoma Cells ◽  
Mcf 7

Novel bismuth(iii) halide compounds were synthesized. Molecules with lower H-all atoms inter-molecular interactions tend to exhibit the higher activity against MCF-7 and HeLa cells.

scholarly journals The Effectiveness of Moringa oleifera Leaf Extracts on The Growth and Yield of Tomato (Solanum lycopersicon)

2020 ◽  
pp. 125-129
Author(s):  
Zannah Kyari ◽  
Ahmed Bunu ◽  
Idris Dauda ◽  
Ibrahim Baba Shehu
Keyword(s):  
Plant Height ◽  
Leaf Extract ◽  
Arid Environment ◽  
Growth And Yield ◽  
Growth Stages ◽  
Plant Leaves ◽  
Leaf Extracts ◽  
Solanum Lycopersicum L ◽  
Research And Teaching ◽  
Significant Difference

The experiment was conducted at the Department of Agricultural Technology Research and Teaching Farm in Ramat Polytechnic Maiduguri to determine the effect of Moringa olifera leaf extract on the growth and yield of tomatoes (Solanum lycopersicum L.) in the semi-arid environment of Maiduguri, Borno State. Parameters measure include, leaf number, plant height, number of fruit and weight of fruits. The result obtained shows that there was significant difference in plant height per plant among the treatment at 4 and 8 WAT, while number of plant leaves per plant was recorded highest in Treatment three (T3) between other treatment. This study suggests the application of Moringa olifera leaf extract at critical growth stages for better growth and yield of performance of tomatoes production in Maiduguri is the best as it gives highest yield and highly profitable when compared to other source of plant extract.

scholarly journals Evaluation of qualitative phytochemicals and antifungal activity of aqueous leaf extract of Senna tora on downy mildew of cabbage

2018 ◽  
Vol 11 (1) ◽  
pp. 15-19
Author(s):  
S.M. Zakari ◽  
A.A. Aliko ◽  
H Haruna ◽  
A Muazu
Keyword(s):  
Downy Mildew ◽  
Leaf Extract ◽  
Flavonoid Glycosides ◽  
Phytochemical Analysis ◽  
Zone Of Inhibition ◽  
Active Metabolites ◽  
Leaf Extracts ◽  
Minimum Fungicidal Concentration ◽  
Significant Difference ◽  
Aqueous Leaf Extract

This study was embarked upon to evaluate the efficacy of aqueous extracts of Senna tora leaf against Perenospora parasitica, causing downy mildew on cabbage. Phytochemical analysis of the leaf extracts revealed the presence of some secondary metabolites (Tannins, Saponins, Flavonoid, Glycosides, Alkaloids, Phenolic compounds and Carbohydrates) which have been reported to have antimicrobial effects. Assays were performed using extract concentrations of 1000, 500, 250, 125 mg/ml by agar well diffusion technique. Results obtained, revealed a significant difference in diameter of mycelial zone of inhibition between the concentrations. Highest zone of inhibition was recorded at 1000 and 500 mg/ml (20 and 15 mm) respectively. Minimum Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration (MFC) were determined and recorded at 250 mg/ml. The antifungal screening revealed that the extract exhibited inhibitory activities at varying concentrations against the downy mildew pathogen. These activities observed could be attributed to the presence of active metabolites contained in the extract.Keywords: Aqueous leaf extract, Downy mildew, Perenospora parasitica and Senna tora

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