scholarly journals Biodegradation of Alprazolam in Pharmaceutical Wastewater Using Mesoporous Nanoparticles-Adhered Pseudomonas stutzeri

Molecules ◽  
2021 ◽  
Vol 27 (1) ◽  
pp. 237
Author(s):  
Mahdi Shahriarinour ◽  
Faten Divsar ◽  
Fereshteh Kamalpour Dahka ◽  
Sharareh Nezamivand Chegini ◽  
Mohamad Mahani ◽  
...  
Keyword(s):  
Bacterial Strain ◽  
Mesoporous Silica Nanoparticles ◽  
Pseudomonas Stutzeri ◽  
Degradation Process ◽  
Inorganic Nanoparticles ◽  
Bacterial Cells ◽  
Pharmaceutical Wastewater ◽  
Bacterial Immobilization ◽  
Freely Suspended ◽  
Quantitative Hplc Analysis

The release of pharmaceutical wastewaters in the environment is of great concern due to the presence of persistent organic pollutants with toxic effects on environment and human health. Treatment of these wastewaters with microorganisms has gained increasing attention, as they can efficiently biodegrade and remove contaminants from the aqueous environments. In this respect, bacterial immobilization with inorganic nanoparticles provides a number of advantages, in terms of ease of processing, increased concentration of the pollutant in proximity of the cell surface, and long-term reusability. In the present study, MCM-41 mesoporous silica nanoparticles (MSN) were immobilized on a selected bacterial strain to remove alprazolam, a persistent pharmaceutical compound, from contaminated water. First, biodegrading microorganisms were collected from pharmaceutical wastewater, and Pseudomonas stutzeri was isolated as a bacterial strain showing high ability to tolerate and consume alprazolam as the only source for carbon and energy. Then, the ability of MSN-adhered Pseudomonas stutzeri bacteria was assessed to biodegrade alprazolam using quantitative HPLC analysis. The results indicated that after 20 days in optimum conditions, MSN-adhered bacterial cells achieved 96% biodegradation efficiency in comparison to the 87% biodegradation ability of Pseudomonas stutzeri freely suspended cells. Kinetic study showed that the degradation process obeys a first order reaction. In addition, the kinetic constants for the MSN-adhered bacteria were higher than those of the bacteria alone.

scholarly journals Pyridine-2,6-Bis(Thiocarboxylic Acid) Produced by Pseudomonas stutzeri KC Reduces and Precipitates Selenium and Tellurium Oxyanions

2006 ◽  
Vol 72 (5) ◽  
pp. 3119-3129 ◽  
Author(s):  
Anna M. Zawadzka ◽  
Ronald L. Crawford ◽  
Andrzej J. Paszczynski
Keyword(s):  
Wild Type Strain ◽  
Hydrolysis Product ◽  
Pseudomonas Stutzeri ◽  
Elemental Selenium ◽  
Bacterial Cells ◽  
Wild Type ◽  
Chemical Structures ◽  
Thiocarboxylic Acid ◽  
Insoluble Compounds ◽  
Initial Line

ABSTRACT The siderophore of Pseudomonas stutzeri KC, pyridine-2,6-bis(thiocarboxylic acid) (pdtc), is shown to detoxify selenium and tellurium oxyanions in bacterial cultures. A mechanism for pdtc's detoxification of tellurite and selenite is proposed. The mechanism is based upon determination using mass spectrometry and energy-dispersive X-ray spectrometry of the chemical structures of compounds formed during initial reactions of tellurite and selenite with pdtc. Selenite and tellurite are reduced by pdtc or its hydrolysis product H2S, forming zero-valent pdtc selenides and pdtc tellurides that precipitate from solution. These insoluble compounds then hydrolyze, releasing nanometer-sized particles of elemental selenium or tellurium. Electron microscopy studies showed both extracellular precipitation and internal deposition of these metalloids by bacterial cells. The precipitates formed with synthetic pdtc were similar to those formed in pdtc-producing cultures of P. stutzeri KC. Culture filtrates of P. stutzeri KC containing pdtc were also active in removing selenite and precipitating elemental selenium and tellurium. The pdtc-producing wild-type strain KC conferred higher tolerance against selenite and tellurite toxicity than a pdtc-negative mutant strain, CTN1. These observations support the hypothesis that pdtc not only functions as a siderophore but also is involved in an initial line of defense against toxicity from various metals and metalloids.

scholarly journals Anthracene and Pyrene Biodegradation Performance of Marine Sponge Symbiont Bacteria Consortium

Molecules ◽  
2021 ◽  
Vol 26 (22) ◽  
pp. 6851
Author(s):  
Ismail Marzuki ◽  
Ruzkiah Asaf ◽  
Mudian Paena ◽  
Admi Athirah ◽  
Khairun Nisaa ◽  
...  
Keyword(s):  
Marine Sponge ◽  
Bacterial Species ◽  
Pseudomonas Stutzeri ◽  
Acinetobacter Calcoaceticus ◽  
Bacterial Consortium ◽  
Processing Plant ◽  
Petroleum Processing ◽  
Bacterial Cells ◽  
Polycyclic Aromatic ◽  
Biological Methods

Every petroleum-processing plant produces sewage sludge containing several types of polycyclic aromatic hydrocarbons (PAHs). The degradation of PAHs via physical, biological, and chemical methods is not yet efficient. Among biological methods, the use of marine sponge symbiont bacteria is considered an alternative and promising approach in the degradation of and reduction in PAHs. This study aimed to explore the potential performance of a consortium of sponge symbiont bacteria in degrading anthracene and pyrene. Three bacterial species (Bacillus pumilus strain GLB197, Pseudomonas stutzeri strain SLG510A3-8, and Acinetobacter calcoaceticus strain SLCDA 976) were mixed to form the consortium. The interaction between the bacterial consortium suspension and PAH components was measured at 5 day intervals for 25 days. The biodegradation performance of bacteria on PAH samples was determined on the basis of five biodegradation parameters. The analysis results showed a decrease in the concentration of anthracene (21.89%) and pyrene (7.71%), equivalent to a ratio of 3:1, followed by a decrease in the abundance of anthracene (60.30%) and pyrene (27.52%), equivalent to a ratio of 2:1. The level of pyrene degradation was lower than that of the anthracene due to fact that pyrene is more toxic and has a more stable molecular structure, which hinders its metabolism by bacterial cells. The products from the biodegradation of the two PAHs are alcohols, aldehydes, carboxylic acids, and a small proportion of aromatic hydrocarbon components.

scholarly journals ANTAGONISTIC ACTIVITY OF PAENIBACILLUS POLYMYXA, BACILLUS AMYLOLIQUEFACIENS AND THEIR LASER STIMULATION AGAINST PHYTOPATHOGENS

2021 ◽  
Vol 3 (27) ◽  
pp. 135-134
Author(s):  
M.V. Maslova ◽  
◽  
E.V. Grosheva ◽  
A.V. Budagovsky ◽  
O.N. Budagovskaya ◽  
...  
Keyword(s):  
Laser Irradiation ◽  
Pseudomonas Syringae ◽  
Bacterial Strain ◽  
Bacillus Amyloliquefaciens ◽  
Paenibacillus Polymyxa ◽  
Culture Method ◽  
Antagonistic Activity ◽  
Light Irradiation ◽  
Bacterial Cells ◽  
Coherent Light

Nowadays, thanks to organic farming development, particular attention is paid to the biological methods of plants protection, especially from diseases. Therefore, new biological preparations creation and studying their effectiveness are promising directions. The aim of the research was to study the antagonistic activity of the bacteria Paenibacillus polymyxa and Bacillus amyloliquefaciens against some phytopathogens (Pseudomonas syringae van Hall, Fusarium oxysporum Schltdl, Alternaria alternata (Fr.) Keissl.), as well as their reaction to laser irradiation. The research was carried out in the Research Problem Laboratory “Biphotonika” of the Michurinsk State Agrarian University and the Department of Agricultural Microbiology of the Research Institute of Agriculture of Crimea in 2016–2021. The antagonistic activity of the bacteria was evaluated by the double culture method. The effectiveness of irradiation (as a result of their treatment with coherent light) was determined by the change in the number of bacterial cells in the suspension. The study of the activity of bacteria-antagonists against pathogens showed that the suppression of P. syringae, F. oxysporum, A. alternata growth in the double culture with P. polymyxa compared to the control variants was 32.5 %; 4.0 % and 77.9 %, respectively. B. amyloliquefaciens suppressed the growth of P. syringae by 25.9 %, F. oxysporum – by 49.0 %; A. alternate – by 61.1 %. An increase in the number of cells in suspensions of P. polymyxa and B. amyloliquefaciens after coherent light irradiation by 26.6 % and 36.7 %, respectively, was also found. Thus, to control P. syringae, bacterial strain of P. polymyxa P was more effective. The bacterial strain B. amyloliquefaciens 01-1 showed antagonistic activity against F. oxysporum. Both studied microorganisms were effective against A. alternata. To increase the activity of cell division of antagonist strains of phytopathogens, it is advisable to use coherent light irradiation. In future, it is advisable to test the level of antagonistic activity of the bacteria P. polymyxa and B. amyloliquefaciens after laser irradiation.

scholarly journals Novel carbonatogenic bacterial strain isolated from limestone quarry in East Java, Indonesia to improve concrete performance

2021 ◽  
Vol 22 (9) ◽  
Author(s):  
Enny Zulaika ◽  
Muhammad Andry Prio Utomo ◽  
Ajeng Selvyana Pangestu ◽  
Nur Hidayatul Alami ◽  
Maya Shovitri ◽  
...  
Keyword(s):  
Bacterial Strain ◽  
Concrete Strength ◽  
Concrete Structures ◽  
Maximum Load ◽  
Rrna Gene ◽  
Bacterial Cells ◽  
Bacterial Strains ◽  
X Ray Diffraction ◽  
Limestone Quarry ◽  
Crystal Properties

Abstract. Zulaika E, Utomo MAP, Pangestu AS, Alami NH, Shovitri M, Prasetyo EN, Setiawan E, Luqman A, Kuswytasari ND, Irawan C. 2021. Novel carbonatogenic bacterial strain isolated from limestone quarry in East Java, Indonesia to improve concrete performance. Biodiversitas 22: 3890-3898. Carbonatogenic bacteria can precipitate CaCO3 in the form of calcite, aragonite, or vaterite. Calcite has the potential to be applied for strengthening concrete structures. This research aims to explore several new bacterial strains that can precipitate calcium carbonate leading to produce calcite and could be useful for strengthening concrete structures. Soil and stalactite samples were taken from a well-known limestone quarry in East Java, Indonesia. The isolated bacteria species were identified using 16S rRNA gene sequences. CaCO3 crystal properties were characterized using X-Ray Diffraction and Scanning Electron Microscopy. Six novels isolated CaCO3 precipitating bacterial strains; Bacillus huizhouensis JA1; B. galactosidilyticus JB3; B. niacini AK4, B. lentus SU1, Lysinibacillus macroides JB2, and Sporosarcina soli JA4 were successfully isolated and have the potential to enhance concrete strength. All isolates were able to produce CaCO3 in calcite form except B. galactosidilyticus JB3. The experimental concrete with the addition of bacterial cells showed higher compressive strength and maximum load compared to control concrete and met the requirements for building construction so that it could be applied for building structure materials.

Concepts on Smart Nano-Based Drug Delivery System

2021 ◽  
Vol 15 ◽  
Author(s):  
Shweta Rai ◽  
Neeraj Singh ◽  
Sankha Bhattacharya
Keyword(s):  
Drug Delivery ◽  
Drug Delivery System ◽  
Delivery System ◽  
Polymeric Nanoparticles ◽  
Mesoporous Silica Nanoparticles ◽  
High Specificity ◽  
Inorganic Nanoparticles ◽  
Information Storage ◽  
Passive Targeting ◽  
Hydrophilic Drugs

Nanomedicine is a branch of healthcare, which has many clinical applications. Nanoscale science has to presume an attractive slot for research in drug delivery as nanocarriers either through active or passive targeting approaches to the cell-specific drug delivery. Liposomes, solid-lipid nanoparticles, dendrimers, polymeric nanoparticles, mesoporous silica nanoparticles, inorganic nanoparticles, nanotubes, quantum dots, and nanofibers are nanocarriers that are proved too smart nano-based drug delivery system. The nanoparticulate system shows high stability, high specificity, high efficacy, liability to form in different dosage, used through different routes, as well as the ability to deliver hydrophobically as well as hydrophilic drugs. These nanoparticulate systems are showing wider applications to cure the disease through nanomedicines and biomedical applications viz. chemical and biological sensors, information storage systems, magneto-optic, optical devices, and fiber-optic systems. In this review article, the author describes the various smart nano-based drug delivery system along with their endocytic pathways used for the uptake of nanoparticles.

Degradable and colloidally stable zwitterionic-functionalized silica nanoparticles

Nanomedicine ◽  
2021 ◽  
Vol 16 (2) ◽  
pp. 85-96
Author(s):  
Andressa C Schneid ◽  
Iris RS Ribeiro ◽  
Flávia E Galdino ◽  
Jefferson Bettini ◽  
Mateus B Cardoso
Keyword(s):  
Silica Nanoparticles ◽  
Colloidal Stability ◽  
Mesoporous Silica Nanoparticles ◽  
Degradation Process ◽  
Functionalized Silica ◽  
Biologically Relevant ◽  
Unstable Particles ◽  
Biological Compatibility ◽  
Full Optimization ◽  
Functionalized Silica Nanoparticles

Aim: This work is focused on obtaining degradable mesoporous silica nanoparticles (DMSNs) which are able to maintain their colloidal stability in complex biological media. Materials & methods: DMSNs were synthesized using different ratios of disulfide organosilane (degradable structural moiety) and further functionalized with sulfobetaine silane (SBS) to enhance colloidal stability and improve biological compatibility. Results: There was a clear trade-off between nanoparticle degradability and colloidal stability, since full optimization of the degradation process generated unstable particles, while enhancing colloidal stability resulted in poor DMSNs degradation. It was also shown that acidic pH improved particle degradation which is commonly triggered by reduction stimulus. Conclusion: A chemical composition window was found where DMSNs presented satisfactory colloidal stability in biologically relevant medium, meaningful degradation profiles and high biocompatibility.

scholarly journals Identification of Klebsiella Variicola T29A Genes Involved In Tolerance To Desiccation

2019 ◽  
Vol 13 (1) ◽  
pp. 256-267 ◽  
Author(s):  
Osvaldo Rodríguez-Andrade ◽  
Andrés Corral-Lugo ◽  
Yolanda E. Morales-García ◽  
Verónica Quintero-Hernández ◽  
América P. Rivera-Urbalejo ◽  
...  
Keyword(s):  
Bacterial Strain ◽  
Abiotic Factors ◽  
Random Mutagenesis ◽  
Protein Domain ◽  
Bacterial Cells ◽  
Bacterial Survival ◽  
Tn5 Transposon ◽  
Genes Encoding ◽  
Type Gene ◽  
Corn Plants

Introduction: Several plant-beneficial bacteria have the capability to promote the growth of plants through different mechanisms. The survival of such bacteria could be affected by environmental abiotic factors compromising their capabilities of phytostimulation. One of the limiting abiotic factors is low water availability. Materials and Methods: In extreme cases, bacterial cells can suffer desiccation, which triggers harmful effects on cells. Bacteria tolerant to desiccation have developed different strategies to cope with these conditions; however, the genes involved in these processes have not been sufficiently explored. Klebsiella variicola T29A is a beneficial bacterial strain that promotes the growth of corn plants and is highly tolerant to desiccation. In the present work, we investigated genes involved in desiccation tolerance. Results & Discussion: As a result, a library of 8974 mutants of this bacterial strain was generated by random mutagenesis with mini-Tn5 transposon, and mutants that lost the capability to tolerate desiccation were selected. We found 14 sensitive mutants; those with the lowest bacterial survival rate contained mini-Tn5 transposon inserted into genes encoding a protein domain related to BetR, putative secretion ATPase and dihydroorotase. The mutant in the betR gene had the lowest survival; therefore, the mutagenized gene was validated using specific amplification and sequencing. Conclusion: Trans complementation with the wild-type gene improved the survival of the mutant under desiccation conditions, showing that this gene is a determinant for the survival of K. variicola T29A under desiccation conditions.

scholarly journals Biocoatings: Painting bacteria on surfaces

2020 ◽  
Vol 2 (7A) ◽  
Author(s):  
Simone Krings ◽  
Yuxiu Chen ◽  
Suzie Hingley-Wilson ◽  
Joseph L. Keddie
Keyword(s):  
Laser Scanning ◽  
Fluorescent Protein ◽  
Yellow Fluorescent Protein ◽  
Inorganic Nanoparticles ◽  
Laser Scanning Microscopy ◽  
Polymer Materials ◽  
Latex Film ◽  
Confocal Laser ◽  
Bacterial Cells ◽  
E Coli

Background: Biocoatings are nanoporous polymer materials which encapsulate bacterial cells with carbohydrates as osmoprotectants. Here, we optimised biocoatings to offer a favourable environment for the metabolic activity of bacteria. Methods: E. coli were used as a model organism and mixed with the colloidal polymer particles (i.e. synthetic latex), inorganic nanoparticles and different carbohydrates. Films were casted and dried to create a coalesced latex film and finally rehydrated to re-establish bacterial metabolism. The toxicity of the sterile latices to the bacteria was tested by using the colourimetric redox indicator resazurin. Visualisation of the bacteria inside the biocoatings was performed by confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM). Results: We introduced halloysite (clay nanotubes) to create nanoporosity, which created voids in the structure that will permit gas exchange. The biocoatings were tested in liquid and rehydrated states with resazurin to find the most promising composition ensuring bacterial viability. Rehydrated biocoatings were visualised by CLSM by tracking the constitutively expressed yellow-fluorescent protein (YFP) for viable cells and the membrane exclusion dye propidium iodide for dead cells. The structure of the biocoatings appeared to be unaffected by freeze-drying compared to chemical fixation. Following this fixation, SEM allowed the observation of the organisation of the latex polymers, halloysite and bacteria. Conclusions: The biocoatings were highly porous thanks to halloysite. E. coli survived the film formation process. Next, we will use E. coli and cyanobacteria to achieve higher efficiency for a variety of applications e.g. pollutant degradation, solar energy harvesting and carbon recycling.

scholarly journals Insights into detoxification of tolaasins, the toxins behind mushroom bacterial blotch, by Microbacterium foliorum NBRC 103072T

2021 ◽  
Author(s):  
Shun Tomita ◽  
Akinobu Kajikawa ◽  
Shizunobu Igimi ◽  
Hirosuke Shinohara ◽  
Kenji Yokota
Keyword(s):  
Degradation Process ◽  
Detergent Solution ◽  
Bacterial Cells ◽  
Biological Degradation ◽  
Pseudomonas Tolaasii ◽  
Cyclic Lipopeptides ◽  
Peptide Moiety ◽  
Brown Blotch ◽  
Brown Blotch Disease ◽  
Detoxification Process

Tolaasins are lipodepsipeptides secreted by Pseudomonas tolaasii, the causal agent of brown blotch disease of mushrooms, and are the toxins that cause the brown spots. We previously reported that Microbacterium foliorum NBRC 103072T is an effective tolaasin-detoxifying bacterium. In this study, we aimed to characterize the tolaasin-detoxification process of M. foliorum NBRC 103072T. The tolaasin-detoxification by M. foliorum NBRC 103072T was carried out by hydrolyzation of tolaasins at two specific sites in the peptide moiety of tolaasins by its cells, and the resulting fragments were released from bacterial cells. The tolaasin-hydrolyzing activity can be extracted by neutral detergent solution from M. foliorum NBRC 103072T cells. Moreover, tolaasin-adsorption to the bacterial cells occurred prior to hydrolyzation of tolaasins, which might contribute to the effective tolaasin-detoxification by M. foliorum NBRC 103072T. It is notable that the tolaasin-degradation process by M. foliorum NBRC 103072T is carried out by hydrolyzation at specific sites in the peptide moiety of lipopeptide by bacterial cells as a novel biological degradation process of cyclic lipopeptides.

Selenium recovery from kiln powder of cement manufacturing by chemical leaching and bioreduction

2015 ◽  
Vol 72 (8) ◽  
pp. 1294-1300 ◽  
Author(s):  
S. Soda ◽  
A. Hasegawa ◽  
M. Kuroda ◽  
A. Hanada ◽  
M. Yamashita ◽  
...  
Keyword(s):  
Bacterial Strain ◽  
Pseudomonas Stutzeri ◽  
Treated Wastewater ◽  
Elemental Selenium ◽  
Chemical Leaching ◽  
Slurry Concentration ◽  
Effluent Concentration ◽  
Cement Manufacturing

A novel process by using chemical leaching followed by bacterial reductive precipitation was proposed for selenium recovery from kiln powder as a byproduct of cement manufacturing. The kiln powder at a slurry concentration of 10 w/v% with 0.25 M Na2CO3 at 28°C produced wastewater containing about 30 mg-Se/L selenium. The wastewater was diluted four-fold and adjusted to pH 8.0 as preconditioning for bioreduction. A bacterial strain Pseudomonas stutzeri NT-I, capable of reducing selenate and selenite into insoluble elemental selenium, could recover about 90% selenium from the preconditioned wastewater containing selenium of 5 mg-Se/L when supplemented with lactate or glycerol. The selenium concentrations in the treated wastewater were low around the regulated effluent concentration of 0.1 mg-Se/L in Japan.

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