scholarly journals Decreased Efficiency of Very-Low-Density Lipoprotein Lipolysis Is Linked to Both Hypertriglyceridemia and Hypercholesterolemia, but It Can Be Counteracted by High-Density Lipoprotein

Nutrients ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 1224
Author(s):  
Ewa Wieczorek ◽  
Agnieszka Ćwiklińska ◽  
Agnieszka Kuchta ◽  
Barbara Kortas-Stempak ◽  
Anna Gliwińska ◽  
...  
Keyword(s):  
High Density Lipoprotein ◽  
Electrophoretic Mobility ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
High Density ◽  
Lipid Lowering ◽  
Lower Percentage ◽  
Very Low Density Lipoprotein ◽  
Low Density ◽  
Vldl Triglyceride

Impaired triglyceride-rich lipoprotein plasma catabolism is considered the most important factor for hypertriglyceridemia development. The aim of this study was to evaluate the impact of hypercholesterolemia and hypertriglyceridemia on the efficiency of lipoprotein lipase (LPL)-mediated very-low-density lipoprotein (VLDL)-triglyceride lipolysis and the role of high-density lipoprotein (HDL) in this process. Subjects with no history of cardiovascular disease (CVD) and untreated with lipid-lowering agents were recruited into the study and divided into normolipidemic, hypercholesterolemic, and hyperlipidemic groups. VLDL was isolated from serum and incubated with LPL in the absence or presence of HDL. For the hypercholesterolemic and hyperlipidemic groups, a significantly lower percentage of hydrolyzed VLDL-triglyceride was achieved compared to the normolipidemic group (p < 0.01). HDL enhanced the lipolysis efficiency in the hypercholesterolemic and hyperlipidemic groups on average by ~7% (p < 0.001). The lowest electrophoretic mobility of the VLDL remnants indicating the most effective lipolysis was obtained in the normolipidemic group (p < 0.05). HDL presence significantly reduced the electrophoretic mobility of the VLDL remnants for the hypercholesterolemic and hyperlipidemic groups (p < 0.05). The results of our study indicate that VLDL obtained from hypercholesterolemic and hyperlipidemic subjects are more resistant to lipolysis and are additional evidence of the need for early implementation of hypocholesterolemic treatment, already in asymptomatic CVD subjects.

scholarly journals Effect of Some Strains of Lactic Acid Bacteria and Their Mixture on the Level of Fats and Cholesterol in Albino Rats (Rattus norvegicus) Male with Hypothyroidism Induced Using Carbimazole

2021 ◽  
Vol 34 (1) ◽  
pp. 139-146
Author(s):  
Elham K. Nasser ◽  
Kithar R. Majeed ◽  
Hayder I. Ali
Keyword(s):  
High Density Lipoprotein ◽  
Low Density Lipoprotein ◽  
Control Sample ◽  
Density Lipoprotein ◽  
High Density ◽  
Control Group ◽  
Albino Rats ◽  
Very Low Density Lipoprotein ◽  
Low Density ◽  
Fortified Milk

Fortified milk containing Lactobacillus plantarum, L. casei, and L. acidophilus isolates and their mixture were used in dosing the male albino rats at an age of 9-12 weeks at an average of 23 g with induced hypothyroidism at a concentration of 0.6 g.kg-1 of carbimazole. Total cholesterol, triglycerides, high-density lipoprotein (HDL), low-density lipoprotein (LDL), and very-low-density lipoprotein (VLDL) were estimated. The results showed a significant increase in the level of triglycerides (TG), cholesterol and triglycerides. Low-density lipoprotein (LDL) and very-low-density lipoprotein (VLDL), with a significant decrease in the level of high-density lipoprotein (HDL) in infected male mice, compared to the control sample, and upon dosing with liquid milk fortified, it returned to its normal level without significant differences from the control group.

Evaluation of the dual-precipitation method by comparison with the ultracentrifugation method for measurement of lipoproteins in serum.

1977 ◽  
Vol 23 (7) ◽  
pp. 1238-1244 ◽  
Author(s):  
P N Demacker ◽  
H E Vos-Janssen ◽  
A P Jansen ◽  
A van 't Laar
Keyword(s):  
High Density Lipoprotein ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
High Density ◽  
Lipoprotein Cholesterol ◽  
Precipitation Method ◽  
Very Low Density Lipoprotein ◽  
Low Density ◽  
Low Density Lipoprotein Cholesterol ◽  
Noticeable Effect

Abstract We evaluated the dual-precipitation method for quantitative measurement of lipoproteins as described by Wilson and Spiger [J. Lab. Clin. Med. 82, 473 (1973)] for normo- and hyperlipemic sera, by comparison with the results obtained with ultracentrifugation. If serum with an above-normal triglyceride concentration is analyzed, the very-low-density lipoprotein cholesterol value obtained with the precipitation method is usually too low. For measurement of high-density lipoprotein cholesterol the ultracentrifugation and precipitation procedures give comparable results, but the latter method is preferred because sinking pre-beta-lipoproteins present in the high-density lipoprotein fraction isolated by means of the ultracentrifuge may result in falsely high values for cholesterol in that fraction. Therefore, at least for the determination of very-low-density lipoprotein cholesterol in hyperlipemic serum, the use of an ultracentrifuge remains necessary. Because few laboratories have an ultracentrifuge at their disposal, it seemed important to look at the stability of sera in view of the forwarding of samples. Also, a way of increasing the efficiency of the ultracentrifuge was studied. Sera can be stored for a week at 4 degrees C or for 54 h at room temperature without noticeable effect on lipoprotein values. Moreover, reliable values can be obtained with an ultracentrifugation time of 8 h (0.8 X 10(8) g-min).

Cardioprotective Activity of Agaricus bisporus Against Isoproterenol- Induced Myocardial Infarction in Laboratory Animals

2019 ◽  
Vol 15 (4) ◽  
pp. 401-407 ◽  
Author(s):  
Apoorva Bhushan ◽  
Mayank Kulshreshtha
Keyword(s):  
Myocardial Infarction ◽  
High Density Lipoprotein ◽  
Total Protein ◽  
Alanine Aminotransferase ◽  
Agaricus Bisporus ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
High Density ◽  
Very Low Density Lipoprotein ◽  
Low Density

Background: Agaricus bisporus (A. bisporus) is an edible basidiomycete mushroom native to grasslands in Europe and North America. A. bisporus, commonly known as white button mushroom (WBM), is widely cultivated in most countries, and it constitutes the bulk of all mushrooms consumed in the United States and Australia. Traditionally this fungus has used in the treatment of heart diseases. Also it has anti-ageing property.Mushrooms have been found effective against cancer, cholesterol reduction, stress, insomnia, asthma, allergies and diabetes. Objective: The present research was designed to appraise the cardioprotective activity of a hydroalcoholic extract of Agaricus bisporus (EEAB) on Isoproterenol (ISO) induced myocardial infarction (MI) in Albino Wistar rat. Traditionally, Agaricus bisporus is reported in the treatment of heart diseases, cancer, cerebral stroke and anti-ageing property. Materials and Methods: Wistar rats of different sex were randomly split into five groups namely positive control, negative control, standard, test-1 and test-2 and received distilled water, ISO (85 mg/kg), Simvastatin (10 mg/kg/day, oral) and EEAB (200 and 400 mg/kg/day, p.o.) for 30 days, respectively. MI was induced in rats by ISO at an interval of 24 hrs on 31 and 32 day and on the next day, blood was amassed through retro-orbital plexus for the assessment of biochemical markers (cholesterol, lowdensity lipoprotein, high-density lipoprotein, very low-density lipoprotein, triglycerides, alanine aminotransferase and total protein) and finally, the rats were immolated by cervical dislocation. The heart tissue was reaped instantly, cleaned with chilled isotonic saline and clasped in 10% buffered formalin and used for the histopathological analysis. Results: ISO p.o. administration significantly elevated the cholesterol, low density lipoprotein, very low density lipoprotein, triglycerides, alanine aminotransferase and aspartate aminotransferase levels while it decreases high-density lipoprotein and total protein in plasma and administration of EEAB decreases the level of cholesterol, low-density lipoprotein, very low-density lipoprotein, triglycerides, alanine aminotransferase and aspartate aminotransferase levels while it increases high-density lipoprotein and total protein levels. Pretreatment with EEAB protected the cardiotoxicity induced by ISO. The histopathological findings support the analysis of biochemical parameters, ISO-induced myocardium showed infracted zone with edema, inflammatory cells, lipid droplets, myocardial necrosis and vacuolization of myofibrils which were reduced. Conclusion: It can be an outcome that EEAB possessed cardioprotective activity against experimental and clinical studies of ISO-induced myocardial infarction in rats.

scholarly journals Apolipoprotein C-II and C-III preferably transfer to both high-density lipoprotein (HDL)2 and the larger HDL3 from very low-density lipoprotein (VLDL)

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Azusa Yamazaki ◽  
Ryunosuke Ohkawa ◽  
Yuka Yamagata ◽  
Yuna Horiuchi ◽  
Shao-Jui Lai ◽  
...  
Keyword(s):  
High Density Lipoprotein ◽  
High Performance ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
High Density ◽  
Large Individual ◽  
Very Low Density Lipoprotein ◽  
Low Density ◽  
Triglyceride Hydrolysis ◽  
Apolipoprotein C

AbstractTriglyceride hydrolysis by lipoprotein lipase (LPL), regulated by apolipoproteins C-II (apoC-II) and C-III (apoC-III), is essential for maintaining normal lipid homeostasis. During triglyceride lipolysis, the apoCs are known to be transferred from very low-density lipoprotein (VLDL) to high-density lipoprotein (HDL), but the detailed mechanisms of this transfer remain unclear. In this study, we investigated the extent of the apoC transfers and their distribution in HDL subfractions, HDL2 and HDL3. Each HDL subfraction was incubated with VLDL or biotin-labeled VLDL, and apolipoproteins and lipids in the re-isolated HDL were quantified using western blotting and high-performance liquid chromatography (HPLC). In consequence, incubation with VLDL showed the increase of net amount of apoC-II and apoC-III in the HDL. HPLC analysis revealed that the biotin-labeled apolipoproteins, including apoCs and apolipoprotein E, were preferably transferred to the larger HDL3. No effect of cholesteryl ester transfer protein inhibitor on the apoC transfers was observed. Quantification of apoCs levels in HDL2 and HDL3 from healthy subjects (n = 8) showed large individual differences between apoC-II and apoC-III levels. These results suggest that both apoC-II and apoC-III transfer disproportionately from VLDL to HDL2 and the larger HDL3, and these transfers might be involved in individual triglyceride metabolism.

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