scholarly journals An Investigation of the Relationship between Cyniclomyces guttulatus and Rabbit Diarrhoea

Pathogens ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 880
Author(s):  
Tuanyuan Shi ◽  
Xinlei Yan ◽  
Hongchao Sun ◽  
Yuan Fu ◽  
Lili Hao ◽  
...  
Keyword(s):  
New Zealand ◽  
Body Weight Gain ◽  
Animal Experiments ◽  
Zealand White Rabbit ◽  
Opportunistic Pathogen ◽  
Large Numbers ◽  
New Zealand White Rabbits ◽  
Positive Rate ◽  
Relationship Of ◽  
The Relationship

Cyniclomyces guttulatus is usually recognised as an inhabitant of the gastrointestinal (GI) tract in rabbits. However, large numbers of C. guttulatus are often detected in the faeces of diarrhoeic rabbits. The relationship of C. guttulatus with rabbit diarrhoea needs to be clearly identified. In this study, a C. guttulatus Zhejiang strain was isolated from a New Zealand White rabbit with severe diarrhoea and then inoculated into SPF New Zealand white rabbits alone or co-inoculated with Eimeriaintestinalis, another kind of pathogen in rabbits. Our results showed that the optimal culture medium pH and temperature for this yeast were pH 4.5 and 40–42 °C, respectively. The sequence lengths of the 18S and 26S ribosomal DNA fragments were 1559 bp and 632 bp, respectively, and showed 99.8% homology with the 18S ribosomal sequence of the NRRL Y-17561 isolate from dogs and 100% homology with the 26S ribosomal sequence of DPA-CGR1 and CGDPA-GP1 isolates from rabbits and guinea pigs, respectively. In animal experiments, the C. guttulatus Zhejiang strain was not pathogenic to healthy rabbits, even when 1 × 108 vegetative cells were used per rabbit. Surprisingly, rabbits inoculated with yeast showed a slightly better body weight gain and higher food intake. However, SPF rabbits co-inoculated with C. guttulatus and E. intestinalis developed more severe coccidiosis than rabbits inoculated with C. guttulatus or E. intestinalis alone. In addition, we surveyed the prevalence of C. guttulatus in rabbits and found that the positive rate was 83% in Zhejiang Province. In summary, the results indicated that C. guttulatus alone is not pathogenic to healthy rabbits, although might be an opportunistic pathogen when the digestive tract is damaged by other pathogens, such as coccidia.

scholarly journals Cyniclomyces guttulatus is an opportunistic pathogen in rabbits with coccidiosis

2019 ◽  
Author(s):  
Tuanyuan Shi ◽  
Hongchao Sun ◽  
Yuan Fu ◽  
Lili Hao ◽  
Yongxue Zhou ◽  
...  
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Body Weight Gain ◽  
Clinical Signs ◽  
Opportunistic Pathogen ◽  
Culture Conditions ◽  
Fecal Output ◽  
Large Numbers ◽  
Positive Rate ◽  
Probiotic Microorganism

Abstract Background: Cyniclomyces guttulatus is a common inhabitant of the gastrointestinal tract in rabbits, and large numbers are often present in feces of diarrheic rabbits. However, its relation with rabbit diarrhea has not been clearly identified. Relationship between C. guttulatus and the rabbit are identified in this paper.Methods: YPG (pH 1.5) medium and 96-well culture plate were used to isolate C. guttulatus from rabbit. Automatic microbial growth curve analyser and optical density scanner were used to optimized culture conditions of the C. guttulatus strain in the YPG medium. Microscope observation, PCR and gene sequencing were used to identify the C. guttulatus strain. Animal inoculation with the C. guttulatus strain or co-inoculation with E. intestinalis were used identify relationship between C. guttulatus and the rabbit. Results: A C. guttulatus Zhejiang strain was isolated from a rabbit with diarrhea and the culture conditions in YPG medium were optimized. The sequenced 18S and 26S ribosomal DNA fragments were 1559bp and 632bp, respectively, and showed 99.8% homology with the 18S ribosomal sequence of the NRRL Y-17561 isolate from the dog and 100% homology with the 26S ribosomal sequence of the DPA-CGR1 and CGDPA-GP1 isolates from the rabbit and guinea pig. Our isolate was not pathogenic to healthy SPF rabbits. Instead, rabbits inoculated with the yeast had a slightly better body weight gain and higher food intake. Rabbits co-inoculated with C. guttulatus and the coccidian, E. intestinalis developed more severe coccidiosis as shown by clinical signs, and decreased body weight gain, diarrhea and death, associated with significantly higher fecal output of C. guttulatus vegetative cells but lower coccidian oocysts output than the rabbits inoculated with C. guttulatus or E. intestinalis alone. We also surveyed the prevalence of C. guttulatus in rabbits and found a positive rate of 83% in Zhejiang province. Conclusions: Our results indicate that C. guttulatus alone is not pathogenic to healthy rabbits and seems a probiotic microorganism in rabbits, but could become an opportunistic pathogen when the digestive tract is damaged by other pathogens such as coccidia.

Australian and New Zealand Biosolids Partnership: Collaboration for more effective biosolids management

2010 ◽  
Vol 5 (2) ◽  
Author(s):  
Andrew Speers ◽  
Allen Gale ◽  
Nancy Penney
Keyword(s):  
New Zealand ◽  
Policy Development ◽  
Sustainable Management ◽  
Best Practice ◽  
Community Consultation ◽  
Critical Research ◽  
Community Attitudes ◽  
Relationship Of ◽  
The Relationship ◽  
Sustainability Framework

This paper describes an international biosolids management initiative, known as the Australian and New Zealand Biosolids Partnership (ANZBP). The ANZBP - known formerly as the Australasian Biosolids Partnership – comprises 33 members dedicated to promoting the sustainable management of biosolids across the two nations. Two critical research projects are described, each of which contributes to the ANZBP goal of promoting the sustainable management of biosolids. The first is a review of community attitudes to biosolids management, the outcomes of which will be used to refine communication tools and methods of community consultation and which will provide input to policy development over time. The second is a review of regulations in place in Australia and New Zealand carried out to identify inconsistencies and improvements that could be made. An outcome of this initiative is potentially the development of a best practice manual. The relationship of the two projects to a sustainability framework adopted by the ANZBP is also described, as is the relationship of the two projects to each other.

scholarly journals The effect of age, anatomical site and bone structure on osteogenesis in New Zealand White rabbit

2015 ◽  
Vol 88 (2) ◽  
Author(s):  
Francesca Ravanetti ◽  
Edoardo Scarpa ◽  
Vittorio Farina ◽  
Marco Zedda ◽  
Carlo Galli ◽  
...  
Keyword(s):  
New Zealand ◽  
Bone Structure ◽  
Skeletal Maturity ◽  
Zealand White Rabbit ◽  
Anatomical Site ◽  
Bone Implants ◽  
New Zealand White Rabbits ◽  
Preclinical Trials ◽  
Parietal Bones

Among animal models, rabbits are widely used in medical research, as they fill the gap between smaller models, commonly employed in basic science, and larger ones, which are better suited for preclinical trials. Given their rapid growth, rabbits provide a valuable system for the evaluation of bone implants for tissue regeneration. By means of a histomorphometric analysis, here we quantified the mineral apposition rates (MARs) in osteonic, periosteum and endosteum osteogenic fronts, of skeletal elements within femur, tibia, radius, ulna, frontal and parietal bones in New Zealand White rabbits aged 6, 7 and 8 months. Our hypothesis is that the MAR varies according to the skeletal maturity of the animal, and also within the skeletal elements and the osteogenic fronts considered. In the present study we show that the MAR in both femur and tibia is significantly higher than in ulna and radius. We also demonstrate that the MAR in parietal bones is significantly higher compared to the MAR of both frontal and forelimb bones. Contrary to what was expected, the MARs of all the skeletal elements considered were not decreased following full skeletal maturity. Finally, the MAR of the osteonic-osteogenic front is the lowest in all of the skeletal elements considered. In conclusion, these results provide new important insights for the evaluation of bone implants, casting a light on the role of both age and osteogenic fronts on the bone MAR, and providing valuable information on the physiological bone turnover in New Zealand White rabbits.

scholarly journals Niue's Relationship of Free Association with new Zealand

1999 ◽  
Vol 30 (2) ◽  
pp. 589 ◽  
Author(s):  
Alison Quentin-Baxter
Keyword(s):  
New Zealand ◽  
Free Association ◽  
General Terms ◽  
Relationship Of ◽  
The Relationship ◽  
Administrative Assistance

This article provides an account of the relationship between Niue and New Zealand. Because Niue is both a self-governing state and one who has a relationship of free association with New Zealand, Niue's government makeup is both constitutional and contractual. The article discusses the principles of Niue's free association status with New Zealand in light of its constitutional makeup. The nature of the relationship between New Zealand and Niue is discussed in general terms, and the principles of economic and administrative assistance from New Zealand is explored in greater detail.  

scholarly journals A Genetic Evaluation System for New Zealand White Rabbit Germplasm Resources Based on SSR Markers

Animals ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 1258
Author(s):  
Jiali Li ◽  
Bin Zhao ◽  
Yang Chen ◽  
Bohao Zhao ◽  
Naisu Yang ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
New Zealand ◽  
Evaluation System ◽  
Zealand White Rabbit ◽  
Genetic Evaluation ◽  
Germplasm Resources ◽  
Polymorphic Loci ◽  
Marker Combination ◽  
New Zealand White Rabbits ◽  
Two Generations

At present, there is an abundance of quality domestic rabbit breeds in China. However, due to the lack of technical standards for the genetic evaluation of rabbit germplasm resources, there have been a number of problems, such as poor breed conservation. By studying the genetic diversity of 130 New Zealand white rabbits (regardless of generation), we obtained the best simple sequence repeat (SSR) marker combination. We found that, when using microsatellite markers for the effective genetic evaluation of domestic rabbits, the number of records should be greater than 60 and the marker number more than 22. Through the comparative analysis of 30 combinations of 22 markers, the optimal combination of 22 markers was determined, and the 22 SSR polymorphic loci were distributed on different chromosomes. We performed a genetic analysis of 200 New Zealand white rabbits corresponding to two generations, using the best SSR polymorphic loci combination. There were no significant differences in the genetic diversity parameters between the two generations of rabbits (p > 0.05), indicating that the characteristics of this excellent rabbit germplasm have been effectively preserved. At the same time, we verified that the established method can be used to evaluate the breed conservation of rabbit germplasm resources.

Post-natal growth changes in the fibre cortex and medulla of the new zealand romney and N-type sheep

1959 ◽  
Vol 53 (3) ◽  
pp. 358-368 ◽  
Author(s):  
Janet P. Tibbits
Keyword(s):  
Growth Rate ◽  
New Zealand ◽  
Cortical Area ◽  
Genotypic Effect ◽  
Fibre Area ◽  
Fibre Growth ◽  
Skin Biopsies ◽  
Growth Changes ◽  
Relationship Of ◽  
The Relationship

The relationship between cortical area and fibre area has been examined in the N/N, N/ +, + / +, + /nr and nr/nr genotypes of the New Zealand Romney, using skin biopsies taken at birth, 1, 3 and 16 months of age. The presence of the nr gene was shown to reduce significantly the relative cortical area. Within the genotypes there was an increase in relative cortical area with increasing age and in two-tooth ewes of N/N and N/ + there was an increase in relative cortical area in winter compared to summer.Fibre growth rates in length for primaries and for prenatal and post-natal secondaries were found for lambs between 1 and 3 months and for 16-monthold ewes. In N/N, N/+ and nr/nr primary fibre growth tended to be increased and post-natal secondary fibre growth tended to be decreased relative to + / + values.In both cortical area and fibre growth rate the greatest genotypic effect was shown by the primary fibres.Some estimates of cortical volume have been made by combining the information on cortical area and fibre growth rate and the relationship of these factors to follicle density and mean fibre area has been discussed. Finally, an indication has been given of how the associations of all these four factors might be usefully examined in some British breeds.

scholarly journals Siderocytes in mammalian blood

1946 ◽  
Vol 133 (872) ◽  
pp. 235-248 ◽  
Keyword(s):  
Clinical Medicine ◽  
Close Correlation ◽  
Bile Pigment ◽  
Pigment Formation ◽  
Adverse Conditions ◽  
Large Numbers ◽  
Mammalian Blood ◽  
Normal Human ◽  
Relationship Of ◽  
The Relationship

Since a review of the conditions under which siderocytes appear will support a hypothesis that it is an ageing erythrocyte at least as strongly as Grüneberg’s (1941 a ) theory that it should be considered as a young cell, a search was made in blood films of stored mammalian blood and large numbers of siderocytes were found. The conditions affecting the rate of appearance of the siderocytes were studied, and it was found that adverse conditions would hasten their appearance. The relationship of the siderotic material to the 'easily split’ blood iron was also considered, and it seems probable that both are derived from a special and identical fraction of ‘haemoglobin’, and that this phenomenon is related to an intracorpuscular bile pigment formation. The occurrence of siderocytosis after the ingestion of acetyl phenylhydrazine by a ‘normal’ human being was followed, and a close correlation between siderocytosis, erythrocyte destruction and urinary siderosis as described by Peyton Rous (1918) is shown. The siderocyte extrudes its siderotic granules and reverts to a state at present morphologically indistinguishable from the normal erythrocyte, but appears to be susceptible of phagocytosis at this stage. The application of siderocyte counts to clinical medicine is suggested.

Rostro-carinates and Rostrate Hand-axes

1939 ◽  
Vol 19 (3) ◽  
pp. 282-284
Author(s):  
J. Reid Moir
Keyword(s):  
Wide Distribution ◽  
Prehistoric Archaeology ◽  
Ancestral Form ◽  
Close Resemblance ◽  
Large Numbers ◽  
Relationship Of ◽  
The Relationship ◽  
Lower Palaeolithic

Since the publication, several years ago, of my paper on the relationship of rostro-carinates to certain Lower Palaeolithic handaxes, a great deal more evidence bearing on this matter has come to light. I have been able to examine large numbers of handaxes, found in this country and in very widely separated places abroad, which exhibit, in their profile and in other characteristics, an extraordinarily close resemblance to rostro-carinates. Such palaeolithic specimens I have called rostrate hand-axes, and their number and wide distribution are beyond dispute. If it is a fact that the rostro-carinate is the ancestral form from which the earliest hand-axes were developed, then it would be reasonable to suppose that the oldest group of these, being nearest in time to the rostro-carinate epoch, would contain the largest number of specimens of the rostrate hand-axe type. Moreover, it would be expected that, in the later hand-axe groups, traces of the ancestral form would gradually fade out, and, except for certain specimens of what may be called atavistic form, be eliminated. That is the theory, and it is sometimes the fate of theories to be killed by facts, but in the case under consideration the reverse holds true. For few things in prehistoric archaeology are clearer than that rostrate hand-axes are most numerous in the Early Chelles period, or that the traces of the rostro-carinate form become ever less in evidence in the later epoch of St. Acheul. Though this is the case, however, the matter is not so simple and straightforward as was perhaps at first supposed.

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