scholarly journals Protein Elicitor PeBL1 of Brevibacillus laterosporus Enhances Resistance Against Myzus persicae in Tomato

Pathogens ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 57 ◽  
Author(s):  
Khadija Javed ◽  
Dewen Qiu
Keyword(s):  
Myzus Persicae ◽  
Host Selection ◽  
Leaf Surface ◽  
Tomato Leaf ◽  
Third Generation ◽  
Population Growth Rates ◽  
Tomato Seedlings ◽  
Brevibacillus Laterosporus ◽  
Protein Elicitor ◽  
Chemical Pesticides

Myzus persicae, a destructive aphid of tomato usually managed by chemical pesticides, is responsible for huge annual losses in agriculture. In the current work, a protein elicitor, PeBL1, was investigated for its capacity to induce a defense response against M. persicae in tomato. Population growth rates of M. persicae (second and third generation) decreased with PeBL1 treatments as compared with controls. In a host selection assay, M. persicae showed preference for colonizing control plants as compared to tomato seedlings treated with PeBL1. Tomato leaves treated with PeBL1 gave rise to a hazardous surface environment for M. persicae due to formation of trichomes and wax. Jasmonic acid (JA), salicylic acid (SA), and ethylene (ET) showed significant accumulation in tomato seedlings treated by PeBL1. The following results showed that PeBL1 significantly modified the tomato leaf surface structure to reduce reproduction and deter colonization by M. persicae. Defense processes also included activation of JA, SA, and ET pathways. The study provides evidence for use of PeBL1 in the protection of tomato from M. persicae.

scholarly journals Biocontrol Potential of Purified Elicitor Protein PeBL1 Extracted from Brevibacillus laterosporus Strain A60 and Its Capacity in the Induction of Defense Process against Cucumber Aphid (Myzus persicae) in Cucumber (Cucumis sativus)

Biology ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 179
Author(s):  
Khadija Javed ◽  
Humayun Javed ◽  
Dewen Qiu
Keyword(s):  
Myzus Persicae ◽  
Intrinsic Rate ◽  
Intrinsic Rate Of Increase ◽  
Cucumber Plant ◽  
Cucumber Seedlings ◽  
Brevibacillus Laterosporus ◽  
Protein Elicitor ◽  
Rate Of Increase ◽  
Biocontrol Potential ◽  
Chemical Pesticides

The Cucumber aphid (Myzus persicae), a destructive cucumber aphid usually managed by chemical pesticides, is responsible for enormous annual agricultural losses. A protein elicitor, PeBL1, was investigated in the present work for its ability to induce a defense response against M. persicae in cucumber. The rates of population growth (Intrinsic rate of increase) of M. persicae (second and third generations) decreased with PeBL1-treated cucumber seedlings as compared to positive (water) and negative 70.58 μg mL−1 controls (50 mM Tris-HCl, pH 8.0). In an assay on host selection, M. persicae had a preference for colonizing control plants as compared to the PeBL1-treated cucumber seedlings. The nymphal development time of the aphid was extended with the PeBL1-treated cucumber seedlings. Likewise, fecundity was reduced, with less offspring produced in the PeBL1-treated cucumber seedlings as compared to the positive (water) and negative 70.58 μg mL−1 controls (50 mM Tris-HCl, pH 8.0). The cucumber leaves treated with PeBL1 had a hazardous surface environment for M. persicae, caused by trichomes and wax formation. Jasmonic acid (JA), salicylic acid (SA), and ethylene (ET) levels were significantly higher, exhibiting significant accumulation in the PeBL1-treated cucumber seedlings. The following results showed that PeBL1 considerably altered the height of the cucumber plant and the surface structure of the leaves to minimize M. persicae reproduction, and it prevented colonization. Defensive processes also included the activation of pathways (JA, SA, and ET). This study provides evidence of biocontrol for the use of PeBL1 in cucumber defense against M. persicae.

Host selection by the generalist aphid Myzus persicae (Hemiptera: Aphididae) and its subspecies specialized on tobacco, after being reared on the same host

2005 ◽  
Vol 95 (1) ◽  
pp. 23-28 ◽  
Author(s):  
A.J. Troncoso ◽  
R.R. Vargas ◽  
D.H. Tapia ◽  
R. Olivares-Donoso ◽  
H.M. Niemeyer
Keyword(s):  
Host Plant ◽  
Myzus Persicae ◽  
Host Selection ◽  
Leaf Surface ◽  
Host Finding ◽  
Host Volatiles ◽  
Surface Exploration ◽  
Allocation Of Time ◽  
Aphid Host ◽  
Rearing Effects

AbstractDecision-making during host selection by phytophagous insects has proved to be related to host range, with specialists taking faster decisions than generalists; however, this pattern fails to materialize in some host selection studies performed with aphids. Differences found in testing designs point to rearing effects on aphid host selection. To test whether specialization patterns derive from the nature of the aphid or as a consequence of rearing environment, host selection behaviours were compared between the generalist Myzus persicae (Sulzer) s.s. and its subspecies specialized on tobacco when reared on a common host and offered the choice of an alternative host and a non-host plant. Pre-alighting (host finding and attraction towards host volatiles) and post-alighting (leaf surface exploration and probing) behaviours did not differ between the generalist and the tobacco-specialist, except in the allocation of time to probing behaviour; furthermore, all specialists chose the host on which they performed best. Thus, although the specialist was not faster than the generalist, it showed a higher level of commitment to its preferred host plant.

scholarly journals Winter mortality, development and reproduction in a field population of Myzus persicae (Sulzer) (Hemiptera: Aphididae) in England

1984 ◽  
Vol 74 (4) ◽  
pp. 633-640 ◽  
Author(s):  
R. Harrington ◽  
Cheng Xia-Nian
Keyword(s):  
Myzus Persicae ◽  
Growth And Development ◽  
Population Change ◽  
Leaf Surface ◽  
Winter Survival ◽  
Winter Mortality ◽  
Biological Factors ◽  
Surface Wetness ◽  
South Eastern ◽  
The Relationship

AbstractPopulation change in Myzus persicae (Sulz.) overwintering on spring cabbage in south-eastern England was studied in relation to meteorological and biological factors. Leaf surface wetness and temperature were correlated significantly with rate of population change, but rainfall and wind were not. Growth and development continued slowly throughout the winter, and the relationship with temperature is shown. Hymenopterous parasites, the fungus Entomophthora and predatory spiders did not affect winter survival.

scholarly journals Effect of copper deficiency and of water stress on the microstructure of tomato leaf surface

2013 ◽  
Vol 51 (1-2) ◽  
pp. 119-125 ◽  
Author(s):  
Barbara Dyki ◽  
Jan Borowski ◽  
Waldemar Kowalczyk
Keyword(s):  
Water Stress ◽  
Copper Deficiency ◽  
Leaf Surface ◽  
Tomato Leaf ◽  
Stress Conditions ◽  
Water Deficiency ◽  
Tomato Plants ◽  
Number Of Leaves ◽  
Effect Of Copper

The reaction of tomato plants cv. Tukan F<sub>1</sub> to copper deficiency and to water stress was compared. Plants grown in copper deficiency and in conditions of water stress were significantly smaller than controls. They had also lower turgor. The epidermis cells of the upper side leaf in the plants growing in copper deficiency or water stress conditions were smaller than in control plants. However the stomata and trichomes number of leaves plants with copper or water deficiency grown were bigger in comparision with control. The pores of stomata were always larger in leaves of control plants than in other objects.

scholarly journals LARVACIDAL EFFECT OF SOME PLANT EXTRACTS AGAINST TOMATO LEAF MINER (TUTA ABSOLUTA MEYRICK; LEPIDOPTERA: GELEHIIDAE)

2021 ◽  
Vol 9 (3) ◽  
pp. 101-107
Author(s):  
Pervin Erdogan ◽  
Zemran Mustafa
Keyword(s):  
Mortality Rate ◽  
Plant Extracts ◽  
Tomato Leaf ◽  
Tuta Absoluta ◽  
Leaf Miner ◽  
Tagetes Patula ◽  
Insecticidal Effect ◽  
Larvicidal Effect ◽  
Tomato Leaf Miner ◽  
Chemical Pesticides

Tuta absoluta Meyrick (Lepidoptera: Gelehiidae) is a significant tomato pest, and chemical pesticides are used extensively for its control. Because of the adverse effects of chemical pesticides, researchers have been paying attention more to biopesticides. In this study, the larvicidal effects of the extracts of Tanacetum vulgare (Asteracea), Aleo vera (L.) and (Liliaceae) Tagetes patula L. (Asteracea) was determined and trials were conducted by using leaf dipping and pot methods. The leaf dipping method was conducted in a climate chamber, whereas the potting experiment was performed in the greenhouse. In all of the experiments, second-stage larvae were used to determine the larvicidal effects. According to the results obtained, the highest larvicidal effect and mortality rate was observed at the highest concentration of T. vulgare extract (86%, effect: 86.55%). The lowest mortality rate and the larvicidal effect was observed at the same concentration of T. patula extract (mortality: 79%, effect: 78.55%). In the pot experiment, the highest yield was seen in the Neem Azal T/S, followed by T. vulgare, A. vera and T. patula extracts. On the first day counts, the lowest insecticidal effect was obtained with the lowest concentration of T. patula extract. The extract of A. vera, T. vulgare and T. patula was effective in experiments carried out in pot and laboratory conditions on T. absoluta. It is suggested that more research be done on using these extracts as a biopesticide to control T. absoluta. Keywords: Tomato leaf miner, plant extracts, larvacidal effect.

scholarly journals Endophytic Isaria javanica pf185 Persists after Spraying and Controls Myzus persicae (Hemiptera: Aphididae) and Colletotrichum acutatum (Glomerellales: Glomerellaceae) in Pepper

Insects ◽  
2021 ◽  
Vol 12 (7) ◽  
pp. 631
Author(s):  
Roland Bocco ◽  
Moran Lee ◽  
Dayeon Kim ◽  
Seongho Ahn ◽  
Jin-Woo Park ◽  
...  
Keyword(s):  
Relative Humidity ◽  
Myzus Persicae ◽  
Leaf Surface ◽  
Petri Dish ◽  
Tween 80 ◽  
Leaf Damage ◽  
Colletotrichum Acutatum ◽  
Bottom Part ◽  
Insecticidal Efficacy ◽  
Leaf Surface Area

This study endeavored to sustainably control aphids and anthracnose after spraying endophytic Isaria javanica pf185 under field conditions. Under two different tents; one batch of seedlings was sprayed with a 107 conidia/mL I. javanica pf185 suspension; while another was sprayed with 0.05% Tween 80® in distilled water. Six leaf discs from the top; middle; and bottom part of the plant canopy were weekly collected and placed on moistened filter paper in a Petri dish for insecticidal and antifungal bioassays against Myzus persicae and Colletotrichum acutatum. Differences were noticed from the 18th day after spraying with mortality (86.67 ± 0.57% versus 36.67 ± 0.64%) and leaf damage (13.45 ± 0.03% versus 41.18 ± 0.06%) on fungus-treated and controlled, respectively. The corrected insecticidal efficacy was 20.43, 39.82, 72.32, 66.43 and 70.04%, while the corrected fungicidal efficacy was 26.07, 38.01, 53.35, 29.08 and 41.81% during five successive weeks. A positive correlation was evident between insecticidal efficacy and relative humidity (r2 = 0.620) and temperature (r2 = 0.424), respectively. No correlation was found between antifungal activity and relative humidity (r2 = 0.061) and temperature (r2 = 0), respectively. The entomopathogenic fungus survived on leaf surface area and in tissues after spraying.

scholarly journals PeBL1, a Novel Protein Elicitor from Brevibacillus laterosporus Strain A60, Activates Defense Responses and Systemic Resistance in Nicotiana benthamiana

2015 ◽  
Vol 81 (8) ◽  
pp. 2706-2716 ◽  
Author(s):  
Haoqian Wang ◽  
Xiufen Yang ◽  
Lihua Guo ◽  
Hongmei Zeng ◽  
Dewen Qiu
Keyword(s):  
Pseudomonas Syringae ◽  
Nicotiana Benthamiana ◽  
Fluorescent Protein ◽  
Defense Responses ◽  
Systemic Resistance ◽  
Pcr Analysis ◽  
Content Type ◽  
Brevibacillus Laterosporus ◽  
Protein Elicitor ◽  
Novel Protein

ABSTRACTWe report the identification, characterization, and gene cloning of a novel protein elicitor (PeBL1) secreted fromBrevibacillus laterosporusstrain A60. Through a purification process consisting of ion-exchange chromatography and high-performance liquid chromatography (HPLC), we isolated a protein that was identified by electrospray ionization quadrupole time of flight tandem mass spectrometry (ESI–Q-TOF–MS-MS). The 351-bp PeBL1 gene produces a 12,833-Da protein with 116 amino acids that contains a 30-residue signal peptide. The PeBL1 protein was expressed inEscherichia coli. The recombinant protein can induce a typical hypersensitive response (HR) and systemic resistance inNicotiana benthamiana, like the endogenous protein. PeBL1-treatedN. benthamianaexhibited strong resistance to the infection of tobacco mosaic virus-green fluorescent protein (TMV-GFP) andPseudomonas syringaepv. tabaci compared to controlN. benthamiana. In addition, PeBL1 triggered a cascade of events that resulted in defense responses in plants, including reactive oxygen species (ROS) production, extracellular-medium alkalization, phenolic-compound deposition, and expression of several defense-related genes. Real-time quantitative-PCR analysis indicated that the known defense-related genesPR-1,PR-5,PDF1.2,NPR1, andPALwere upregulated to varying degrees by PeBL1. This research not only provides insights into the mechanism by which beneficial bacteria activate plant systemic resistance, but also sheds new light on a novel strategy for biocontrol using strain A60.

scholarly journals Transmission of Potato virus Y in Tobacco Plants by Myzus persicae nicotianae and M. persicae s.str.

Plant Disease ◽  
2006 ◽  
Vol 90 (6) ◽  
pp. 777-782 ◽  
Author(s):  
Olympia M. Kanavaki ◽  
John T. Margaritopoulos ◽  
Nikolaos I. Katis ◽  
Panagiotis Skouras ◽  
John A. Tsitsipis
Keyword(s):  
Potato Virus ◽  
Myzus Persicae ◽  
Host Selection ◽  
Potato Virus Y ◽  
Virus Transmission ◽  
Transmission Efficiency ◽  
Behavioral Differences ◽  
Tobacco Plants ◽  
Probing Behavior ◽  
Inoculation Access Period

The transmission efficiency of Potato virus Y N (PVYN) from and to tobacco plants by Myzus persicae nicotianae, a tobacco specialist, and M. persicae s.str. (Hemiptera: Aphididae), a generalist, was evaluated. In direct transmission tests, 1,004 spring migrants collected as nymphs from peach and 1,434 wingless females from 12 clonal lineages of both taxa were examined. Both the winged and wingless females were starved before being transferred individually for a 3-min acquisition period on infected plants and then moved to healthy plants for a 10-min inoculation access period. Little variation in transmission efficiency was observed. Although not statistically significant, M. persicae nicotianae was slightly more effective. This may suggest similar genetic properties (e.g., virion receptors in mouthparts) between the taxa in relation to virus transmission. M. persicae s.str. transmitted the virus less efficiently when a shorter inoculation period was given. This may indicate differences in probing behavior during the early phase of host selection. Nevertheless, behavioral differences related to host selection have been found using arena tests with infected and healthy tobacco plants where 180 winged females from each taxon were tested. M. persicae s.str. had significantly higher propensity to transmit PVYN than M. persicae nicotianae (31.9 versus 15.3%).

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