scholarly journals Polydatin Alleviates Diabetes-Induced Hyposalivation through Anti-Glycation Activity in db/db Mouse

Pharmaceutics ◽  
2021 ◽  
Vol 14 (1) ◽  
pp. 51
Author(s):  
Hyung Rae Kim ◽  
Woo Kwon Jung ◽  
Su-Bin Park ◽  
Hwa Young Ryu ◽  
Yong Hwan Kim ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Salivary Gland ◽  
Flow Rate ◽  
Cell Injury ◽  
Salivary Flow ◽  
Salivary Flow Rate ◽  
Therapeutic Drug ◽  
Common Disease ◽  
Submandibular Salivary Gland ◽  
Damage Marker

Polydatin (resveratrol-3-O-β-mono-D-glucoside) is a polyphenol that can be easily accessed from peanuts, grapes, and red wine, and is known to have antiglycation, antioxidant, and anti-inflammatory effects. Diabetes mellitus is a very common disease, and diabetic complications are very common complications. The dry mouth symptom is one of the most common oral complaints in patients with diabetes mellitus. Diabetes mellitus is thought to promote hyposalivation. In this study, we aimed to investigate the improvement effect of polydatin on diabetes-induced hyposalivation in db/db mouse model of type 2 diabetes. We examined salivary flow rate, TUNEL assay, PAS staining, and immunohistochemical staining for AGEs, RAGE, HMGB1, 8-OHdG, and AQP5 to evaluate the efficacy of polydatin in the submandibular salivary gland. Diabetic db/db mice had a decreased salivary flow rate and salivary gland weight. The salivary gland of the vehicle-treated db/db mice showed an increased apoptotic cell injury. The AGEs were highly accumulated, and its receptor, RAGE expression was also enhanced. Expressions of HMGB1, an oxidative cell damage marker, and 8-OHdG, an oxidative DNA damage marker, increased greatly. However, polydatin ameliorated this hypofunction of the salivary gland and inhibited diabetes-related salivary cell injury. Furthermore, polydatin improved mucin accumulation, which is used as a damage marker for salivary gland acinar cells, and decreased expression of water channel AQP5 was improved by polydatin. In conclusion, polydatin has a potent protective effect on diabetes-related salivary gland hypofunction through its antioxidant and anti-glycation activities, and its AQP5 upregulation. This result suggests the possibility of the use of polydatin as a therapeutic drug to improve hyposalivation caused by diabetes.

scholarly journals The Influence of Insulin Dependent Diabetes Mellitus on Dental Caries and Salivary Flow

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
V. K. Gupta ◽  
Seema Malhotra ◽  
Vasuda Sharma ◽  
S. S. Hiremath
Keyword(s):  
Diabetes Mellitus ◽  
Dental Caries ◽  
Flow Rate ◽  
Insulin Dependent Diabetes Mellitus ◽  
Salivary Flow ◽  
Diabetic Group ◽  
Salivary Flow Rate ◽  
Dependent Diabetes Mellitus ◽  
Carious Lesion ◽  
Insulin Dependent

Objective. To assess whether or not there was any change in the dental caries and rate of salivary flow of patients with Insulin Dependent Diabetes Mellitus (IDDM) and the contribution of salivary flow to caries risk in IDDM.Setting. Department of Endocrinology, MS Ramaiah Hospital, Bangalore, India.Design. A comparative cross-sectional descriptive type.Materials and Methods. The sample consisted of two groups: 140 diabetic group (mean age 14.8 yr) and 140 nondiabetic group (mean age 13.7 yr). Dental caries by dmf(t) and dmf(s) indices for primary dentition and DMF(T) and DMF(S) indices was used in permanent dentition to assess the dental caries experience. Both stimulated and unstimulated salivary flow rate were assessed after collection of saliva.Results. In diabetic group 76% had carious lesion and in nondiabetic group 85.3% had carious lesion. Diabetics have lower mean DMFT, DMFS, dmft, and dmfs compared to the nondiabetic group. Diminished unstimulated and stimulated salivary flow rate in diabetic than nondiabetic group.Conclusions. The findings obtained conclude that even though there was reduced salivary flow rate in diabetic group the caries prevalence was low.

scholarly journals The prevalence of hyposalivation in type 2 diabetes mellitus patients at Endocrinology Department, Internal Medicine Sub Department of RSUP Dr Hasan Sadikin Bandung

2013 ◽  
Vol 25 (3) ◽  
Author(s):  
Marini Sundari ◽  
Tenny Setiani Dewi ◽  
Nanny Natalia
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Flow Rate ◽  
Objective Assessment ◽  
Diabetes Mellitus Patient ◽  
Salivary Flow ◽  
Salivary Flow Rate ◽  
Whole Saliva

Introduction: The salivary flow rate reduces Hyposalivation. Type 2 diabetes mellitus patient with hyposalivation have a high risk of various oral complications if untreated adequately. The aim of this study was to find the prevalence of hyposalivation in type 2 diabetes mellitus patient at RSUP Dr Hasan Sadikin Bandung. Methods: This study was a descriptive observational with a cross-sectional approach and consecutive sampling method to 30 samples. The study was performed with objective assessment through measuring the unstimulated salivary flow of the whole saliva using spitting method for 5 minutes. Results: The result of this study shows that the prevalence of hyposalivation on type 2 diabetes mellitus patient is 10%. The mean of salivary flow rate sample with hyposalivation is 0,07 mL/minute. Conclusion: The conclusion of this study shows a low prevalence of hyposalivation in type 2 diabetes mellitus patients at RSUP Dr Hasan Sadikin Bandung in October 2011.

scholarly journals Xerostomia appearance in type 1 diabetes mellitus children in RSUPN dr. Cipto Mangunkusumo Jakarta

2011 ◽  
Vol 23 (1) ◽  
Author(s):  
Adis Tyaning Puspitasari ◽  
Rosiliwati Wihardja ◽  
Jakobus Runkat
Keyword(s):  
Diabetes Mellitus ◽  
Type 1 Diabetes ◽  
Type 1 Diabetes Mellitus ◽  
Flow Rate ◽  
Salivary Flow ◽  
Diabetes Mellitus Type 1 ◽  
Salivary Flow Rate ◽  
Metabolic Disturbances ◽  
Oral Dryness

Diabetes mellitus is a chronic metabolic disease characterized by hyperglycemia due to insulin deficiency. As a result, there will be metabolic disturbances on carbohydrate, fat, and protein. Diabetes mellitus type 1 may occur because of pancreatic B cells damage resulting in decreased secretion of insulin in absolute terms. Xerostomia is the medical term for the subjective complaint of dry mouth due to the lack of saliva and can occur in patients with type 1 diabetes mellitus. The purpose of this study was to obtain data on the salivary flow rate and oral dryness complaints in children with type 1 diabetes mellitus The method of this study was descriptive by survey technique. The sample was obtained by purposive sampling and consisted of 30 children with type 1 diabetes mellitus in RSUPN Dr. Cipto Mangunkusumo Jakarta in April to May 2010. The study was conducted with an objective examination by measuring the salivary flow rate and subjective examination using a questionnaire. The results showed that the salivary flow rate from an average of 30 respondents was below normal values. The most common complaints about the dryness of the mouth cavity were thirst, 24 patients (80.00%), and oral dryness 19 patients (63.33%). The conclusion from this study showed that children with type 1 diabetes mellitus were having oral dryness complaints and the decrease of salivary flow rate.

scholarly journals Effect of diabetes mellitus on periodontal health status, salivary flow rate and salivary pH in patients with chronic periodontitis

2020 ◽  
Vol 32 (2) ◽  
pp. 12-16
Author(s):  
Lekaa Ibraheem ◽  
Ban Ahmmad ◽  
Ayat Dhafer ◽  
Jannat Dhafer
Keyword(s):  
Diabetes Mellitus ◽  
Flow Rate ◽  
Chronic Periodontitis ◽  
Salivary Flow ◽  
Salivary Flow Rate ◽  
Plaque Index ◽  
Diabetic Patients ◽  
Gingival Index ◽  
Pocket Depth ◽  
Significant Difference

Background: Diabetes and periodontitis are considered as chronic diseases with a bidirectional relationship between them. This study aimed to determine and compare the severity of periodontal health status and salivary parameters in diabetic and non-diabetic patients with chronic periodontitis. Materials and Methods: Seventy participants were enrolled in this study. The subjects were divided into three groups: Group I: 25 patients had type 2 diabetes mellitus with chronic periodontitis, Group 2: 25 patients had chronic periodontitis and with no history of any systemic diseases, Group 3: 20 subjects had healthy periodontium and were systemically healthy. Unstimulated whole saliva was collected for measurement of salivary flow rate and pH. All periodontal parameters (plaque index, gingival index, probing pocket depth and clinical attachment level) were recorded for each patient. Results: The results showed that all clinical periodontal parameters were highest in group 1 in comparison with groups 2 and 3. Comparisons between pairs of groups revealed significant differences between groups 1 and 2 for plaque index, gingival index, probing pocket depth and clinical attachment level, and highly significant differences for plaque index, gingival index between groups 2 and 3, and between groups 1 and 3. The salivary flow rate and pH were lower in group 1 compared to groups 2 and 3. Inter-group comparisons of salivary parameters also revealed a significant difference between groups 1 and 2, with a non-significant difference between groups 2 and 3. Conclusion: Type 2 diabetic patients have significantly lower salivary flow rate, pH and present with advanced periodontal destruction compared to healthy patients. Key word: Saliva; periodontitis; diabetes mellitus.

Dental caries in diabetes mellitus: role of salivary flow rate and minerals

2011 ◽  
Vol 25 (3) ◽  
pp. 183-186 ◽  
Author(s):  
Muhammad Jawed ◽  
Syed M. Shahid ◽  
Shah A. Qader ◽  
Abid Azhar
Keyword(s):  
Diabetes Mellitus ◽  
Dental Caries ◽  
Flow Rate ◽  
Salivary Flow ◽  
Salivary Flow Rate

scholarly journals Salivary flow rate and xerostomia in patients with type I and II diabetes mellitus

10.19082/5244 ◽  
2017 ◽  
Vol 9 (9) ◽  
pp. 5244-5249 ◽  
Author(s):  
Amineh Hoseini ◽  
Ali Mirzapour ◽  
Ali Bijani ◽  
Atena Shirzad
Keyword(s):  
Diabetes Mellitus ◽  
Flow Rate ◽  
Salivary Flow ◽  
Salivary Flow Rate ◽  
Type I

THU0228 PROTECTIVE EFFECT OF LEFLUNOMIDE ON THE SALIVARY SECRETION OF SUBMANDIBULAR GLAND IN THE NOD MOUSE

2020 ◽  
Vol 79 (Suppl 1) ◽  
pp. 341-342
Author(s):  
C. Yang ◽  
X. Zheng ◽  
L. Wang ◽  
X. Yang ◽  
X. Li ◽  
...  
Keyword(s):  
T Cells ◽  
Salivary Gland ◽  
Submandibular Gland ◽  
Flow Rate ◽  
Nod Mice ◽  
Sjogren’S Syndrome ◽  
Salivary Flow ◽  
Sjogren's Syndrome ◽  
Salivary Flow Rate ◽  
Control Group

Background:A study of 15 PSS patients showed that leflunomide had no significant effect on the decrease of salivary flow rate and the formation of lymphocytic infiltrates in salivary glands[1]. However, due to the small number of samples included in this study and the small size of human salivary gland biopsies, the therapeutic effect of leflunomide may be underestimated. At present, there is no further study on this issue, the effect of leflunomide on Sjogren’s syndrome is still not clear.Objectives:To investigate the therapeutic effect of leflunomide on salivary gland secretion dysfunction in the NOD mice with Sjogren’s syndrome.Methods:The NOD mice were randomly divided into four groups: preventive drug group, preventive control group, therapeutic drug group, and therapeutic control group. Salivary flow rate was measured after pilocarpine stimulation; After hematoxylin and eosin staining, the average number and area of infiltrating lesions were compared; The percentage of CD3+T, CD4+T, CD8+T, CD44+CD62L-CD4+T, CD19+B, and CD138+B cells in submandibular gland and spleen were detected by flow cytometry; The levels of serum inflammatory factors TNF-a, IL-17A and IL-6 were detected by CBA method.Results:The salivary flow rate (t = -5.81, P<0.001; z =-3.61,P<0.05), the number of infiltrating foci(t=3.95,P<0.01; t=4.94,P<0.001)and the average area of infiltrating foci(t=3.18.61,P<0.05; z=2.35,P<0.05)in the treatment groups were significantly ameliorated. CD4+T cells(t=2.39 P<0.05; t´=3.82 P<0.01)and CD44+CD62L-CD4+T cells(t´=3.53,P<0.05; t´=3.36,P<0.05)in the submandibular gland were significantly decreased. CD3+T(t=6.08, P<0.001; t=2.76,P<0.05),CD4+T(t´=3.73,P<0.05; t=2.39, P<0.05), CD19+B(t=5.88,P<0.001; t´=4.23, P<0.01) and CD138+B (t=4.30, P<0.001; t=4.46, P<0.01) cells in the spleen were also significantly decreased. In addition, the serum IL-17A of the treatment group reduced to lower level(t=4.15,p<0.01;t=3.36,p<0.01),and the TNF-a level of the preventive drug group decreased(t=4.56; p<0.001).Figure 1.Leflunomide reduced lymphocyte infiltration and improved salivary gland function in NOD mice. A-D: Histology of the submandibular glands of NOD mice in control and treated groups. E-H: The comparison of the submandibular gland index, salivary flow rate, number of infiltrating foci and average area of the submandibular gland, between the control and treated groups. (mean±SD; n=7 per group; * P<0.05, ** P<0.01, *** P<0.001).Figure 2.Changes of lymphocyte subsets in submandibular gland and spleen A-L: Frequencies of CD3+ T cells, CD4+ T cells, CD8+ T cells, CD44+ CD62L-CD4+T cells, CD19+ T cells and CD138+ B cells in the SMG tissues and the spleen collected from treated group and control group. (mean±SD; n=7 per group; * P<0.05, ** P<0.01, *** P<0.001).Figure 3.The proinflammatory cytokine levels of TNF-α, IL-17, and IL-6 in the serum samples from the treated and control group. (mean±SD; n=7 per group; * P<0.05, ** P<0.01, *** P<0.001).Conclusion:Leflunomide may prevent and improve salivary gland hypofunction and inhibit immune activation in NOD mice, providing a theoretical basis for evaluating leflunomide in the treatment of Sjogren’s syndrome.References:[1]Van Woerkom JM, Kruize A A, Geenen R, et al. Safety and efficacy of leflunomide in primary Sjögren’s syndrome: a phase II pilot study [J]. Ann. Rheum. Dis, 2007, 66(8): 1026-32.DOI:10.1136/ard.2006.060905.Acknowledgments :The authors thank the Center for Scientific Research of Anhui Medical University for valuable help in our experiment.Disclosure of Interests: :None declared

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