scholarly journals Genetic Diversity of Selected Rice Genotypes under Water Stress Conditions

Plants ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 27
Author(s):  
Mahmoud M. Gaballah ◽  
Azza M. Metwally ◽  
Milan Skalicky ◽  
Mohamed M. Hassan ◽  
Marian Brestic ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Water Stress ◽  
Drought Stress ◽  
Drought Tolerance ◽  
Ssr Markers ◽  
Similarity Index ◽  
Stress Conditions ◽  
Yield Potential ◽  
Drought Tolerant ◽  
Rice Genotypes

Drought is the most challenging abiotic stress for rice production in the world. Thus, developing new rice genotype tolerance to water scarcity is one of the best strategies to achieve and maximize high yield potential with water savings. The study aims to characterize 16 rice genotypes for grain and agronomic parameters under normal and drought stress conditions, and genetic differentiation, by determining specific DNA markers related to drought tolerance using Simple Sequence Repeats (SSR) markers and grouping cultivars, establishing their genetic relationship for different traits. The experiment was conducted under irrigated (normal) and water stress conditions. Mean squares due to genotype × environment interactions were highly significant for major traits. For the number of panicles/plants, the genotypes Giza179, IET1444, Hybrid1, and Hybrid2 showed the maximum mean values. The required sterility percentage values were produced by genotypes IET1444, Giza178, Hybrid2, and Giza179, while, Sakha101, Giza179, Hybrid1, and Hybrid2 achieved the highest values of grain yield/plant. The genotypes Giza178, Giza179, Hybrid1, and Hybrid2, produced maximum values for water use efficiency. The effective number of alleles per locus ranged from 1.20 alleles to 3.0 alleles with an average of 1.28 alleles, and the He values for all SSR markers used varied from 0.94 to 1.00 with an average of 0.98. The polymorphic information content (PIC) values for the SSR were varied from 0.83 to 0.99, with an average of 0.95 along with a highly significant correlation between PIC values and the number of amplified alleles detected per locus. The highest similarity coefficient between Giza181 and Giza182 (Indica type) was observed and are susceptible to drought stress. High similarity percentage between the genotypes (japonica type; Sakha104 with Sakha102 and Sakha106 (0.45), Sakha101 with Sakha102 and Sakha106 (0.40), Sakha105 with Hybrid1 (0.40), Hybrid1 with Giza178 (0.40) and GZ1368-S-5-4 with Giza181 (0.40)) was also observed, which are also susceptible to drought stress. All genotypes are grouped into two major clusters in the dendrogram at 66% similarity based on Jaccard’s similarity index. The first cluster (A) was divided into two minor groups A1 and A2, in which A1 had two groups A1-1 and A1-2, containing drought-tolerant genotypes like IET1444, GZ1386-S-5-4 and Hybrid1. On the other hand, the A1-2 cluster divided into A1-2-1 containing Hybrid2 genotype and A1-2-2 containing Giza179 and Giza178 at coefficient 0.91, showing moderate tolerance to drought stress. The genotypes GZ1368-S-5-4, IET1444, Giza 178, and Giza179, could be included as appropriate materials for developing a drought-tolerant variety breeding program. Genetic diversity to grow new rice cultivars that combine drought tolerance with high grain yields is essential to maintaining food security.

scholarly journals Phenological, Physiological and yield markers as efficient tools to identify drought tolerant rice genotypes in Eastern India

2020 ◽  
Author(s):  
Soumya Kumar Sahoo ◽  
Goutam Kumar Dash ◽  
Arti Guhey ◽  
Mirza Jaynul Baig ◽  
Madhusmita Barik ◽  
...  
Keyword(s):  
Water Stress ◽  
Drought Stress ◽  
Grain Yield ◽  
Stress Conditions ◽  
Eastern Region ◽  
Eastern India ◽  
Drought Tolerant ◽  
Significant Difference ◽  
Rice Genotypes ◽  
Highly Correlated

ABSTRACTRice production is severely threatened by drought stress in Eastern India. To develop drought tolerant varieties, selection of donors for breeding programme is crucial. Twenty one selected rice genotypes including both tolerant and sensitive to drought were grown under well-watered and drought stress conditions in dry seasons of two successive years of 2017 and 2018. Leaf water potential, relative water content displayed significant difference among the genotypes during vegetative screening. At reproductive stage drought screening, days to 50% flowering was delayed in all genotypes except N22 and Anjali (showed early flowering) however grain yield and other yield related traits decreased significantly compared to well watered condition. Correlation analysis of phenological and yield related traits with grain yield revealed that tiller numbers and panicle numbers are highly correlated with grain yield both under well-watered and water stress conditions and contributes maximum towards grain yield. The dendrogram grouped Mahamaya, Sahabhagidhan, Poornima, IBD 1, Hazaridhan, Samleshwari and Danteshwari into one cluster which performed better under water stress conditions and had grain yield more than 1.69 tha−1. Sahabhagidhan, Poornima, Vandana, and N22 displayed tolerance to drought both under vegetative and reproductive conditions which could be a good selection for the breeders to develop drought tolerant rice cultivars for eastern region of India.

scholarly journals Genetic Diversity, Population Structure, and Marker-Trait Association for Drought Tolerance in US Rice Germplasm

Plants ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 530
Author(s):  
Uttam Bhattarai ◽  
Prasanta K. Subudhi
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Drought Tolerance ◽  
Grain Yield ◽  
Ssr Markers ◽  
Harvest Index ◽  
Reproductive Stage ◽  
Trait Association ◽  
Drought Tolerant ◽  
Rice Genotypes

Drought is a major constraint in some rice-growing areas of the United States. Its impact is most severe at the reproductive stage resulting in low grain yield. Therefore, assessment of genetic and phenotypic variation for drought tolerance in US rice germplasm is necessary to accelerate the breeding effort. Evaluation of 205 US rice genotypes for drought tolerance at the reproductive stage revealed tolerant response in rice genotypes Bengal, Jupiter, Cypress, Jazzman, Caffey, and Trenasse. Harvest index and fresh shoot weight were identified as important traits to explain the majority of variability among the genotypes under drought tolerance. Genotyping with 80 SSR markers indicated a low level of genetic diversity in US germplasm. Population structure analysis grouped the genotypes into eight clusters. The genotypes from California, Louisiana, and Arkansas formed distinct subgroups. Texas genotypes were similar to those from Louisiana and Arkansas. Marker-trait association analysis showed significant association of RM570 and RM351 with grain yield, spikelet fertility, and harvest index whereas shoot dry weight showed association with RM302 and RM461. The drought-tolerant genotypes identified in this study and the SSR markers associated with drought tolerance attributes will be helpful for development of improved drought-tolerant rice varieties through marker assisted selection.

scholarly journals Relationship of root aquaporin genes, OsPIP1;3, OsPIP2;4, OsPIP2;5, OsTIP2;1 and OsNIP2;1 expression with drought tolerance in rice

2020 ◽  
Vol 80 (01) ◽  
Author(s):  
Harendra Verma ◽  
Kamalakshmi Devi1 ◽  
Akhil Ranjan Baruah ◽  
Ramendra Nath Sarma
Keyword(s):  
Water Stress ◽  
Drought Stress ◽  
Drought Tolerance ◽  
Water Uptake ◽  
Root Development ◽  
Fold Increase ◽  
Transcript Abundance ◽  
Stress Conditions ◽  
Drought Tolerant ◽  
Relationship Of

Aquaporins are channel proteins that facilitate the passive uptake and transport of water, ions, molecules and regulate the water balance at the plant level. Genes for aquaporins are important since they play a major role in water uptake. The present study was therefore conducted to assess the relationship of aquaporins predominantly expressed in roots in response to drought tolerance. Transcript abundance of five rice aquaporin genes (PIP2;5, PIP1;3, PIP2;4, TIP2;1 and NIP2;1) in five rice genotypes (Banglami, ARC 10372, Inglongkiri, Ranjit and IR 64) under well-watered and drought stress conditions were measured using qRT-PCR. Significant variation in PIP relative transcript abundance in roots was observed between varieties under both well-watered and drought stress conditions. In roots, the expression levels of most OsPIPs were found to be increased in drought stress conditions. OsPIP1;3, OsPIP2;5, OsTIP2;1 and OsNIP2;1 were upregulated more in upland (drought tolerant) rice and Ranjit lowland (moderately drought tolerant) genotypes than drought susceptible lowland IR-64 rice in drought stress conditions and downregulated/unchanged in well-irrigated conditions. The OsPIP genes were identified as candidate genes based on their high expression under drought stress situations and played a significant role in water uptake and root development under water stress conditions. A positive correlation was observed between fold increase in aquaporin expression and degree of drought tolerance which suggest that these aquaporins genes might play an important role in root development under water stress situation to overcome stress.

scholarly journals Evaluation of Rice (Oryza sativa L.) Genotypes for Drought Tolerance at Germination and Early Seedling Stage

2018 ◽  
Vol 16 (1) ◽  
pp. 44-54 ◽  
Author(s):  
M M Islam ◽  
E Kayesh ◽  
E Zaman ◽  
T A Urmi ◽  
M M Haque
Keyword(s):  
Oryza Sativa ◽  
Water Stress ◽  
Drought Stress ◽  
Drought Tolerance ◽  
Evaluation System ◽  
Oryza Sativa L ◽  
Dry Weight ◽  
Seedling Height ◽  
Early Seedling ◽  
Rice Genotypes

Drought stress is a major constraint to the production and yield stability of crops. Rice (Oryza sativa L.) is considered as a drought-sensitive crop species. Within this species, there are considerable varietal differences in sensitivity to this environmental stress. An experiment was conducted at the laboratory of the Department of Agronomy, Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Bangladesh during April to September 2016 to evaluate 100 rice genotypes for drought tolerance during germination and early seedling growth stage. The genotypes were tested against five levels of drought stress imposed by Polyethylene glycol 6000 (PEG-6000) @ 0, 5, 10, 15 and 20%. The experiment was laid out in a complete randomized design with four replications. The results showed that with increasing water stress, germination in all the genotypes decreased from 95.8% in control to 6.6 % in highest stress (20% PEG) level. Seedling height and dry weight also decreased in all rice genotypes with the increase in water stress level. Based on Standard Evaluation System (SES),18 genotypes were selected primarily. Among the 18 genotypes, Ratoil and Chinisakkar showed higher germination index, relative seedling height and relative seedling dry weight than the check drought tolerant BRRI dhan43 at 20% PEG. Beside these, performance of Kumridhan, Pusur and Somondori was also well at this level compared to BRRI dhan43. The genotypes Ratoil, Chinisakkar, Kumridhan, Pusur and Somondori showed the best performance under drought condition. The Agriculturists 2018; 16(1) 44-54

scholarly journals SCREENING RICE (Oryza sativa L.) GENOTYPES FOR RESISTANCE AGAINST DROUGHT

2020 ◽  
Vol 4 (2) ◽  
pp. 78-82
Author(s):  
Mohd Syahmi Salleh ◽  
Ris Amirah Malek ◽  
Rozilawati Shahari ◽  
Mohd Shukor Nordin
Keyword(s):  
Drought Stress ◽  
Plant Height ◽  
Root Length ◽  
Selection Criterion ◽  
Proline Content ◽  
Yield Potential ◽  
Growth Stages ◽  
Physiological Basis ◽  
Drought Tolerant ◽  
Rice Genotypes

Drought is regarded as one of the limiting factors in rice production nationally and globally. The present study was conducted to study morpho-physiological and biochemical responses of rice genotypes to drought stress, to identify potential traits for use as a selection criterion in breeding drought-tolerant rice at seedling stage and finally to identify rice genotype resistant to drought stress for use as parents in future breeding. The experimental design used was a split-plot design with three replications, with drought stress as the main plot and rice genotypes as the sub-plot. The main plots consisted of control (normal irrigation) and drought stress. The sub-plots consisted of twelve rice genotypes namely Apami (V1), Boewani (V2), Basmati 370 (V3), Cica- 4 (V4), Dular (V5), Jarom mas (V6), Kalarata (V7), Biris (V8), Haiboq (V9), Moroberekan (V10), MR 297 (V11) and Aerob 1 (V12). Results showed that drought stress led to a decrease in plant height, leaves size, root length, total dry weight, and number of leaves but an increment in proline content. The genotype Apami and Kalarata were found to accumulate higher proline content indicating potential resistant ability towards drought stress. Dular and Aerob1, along with tolerant control genotype, Moroberekan, on the other hand, recorded a lower SES score. Leave size, root length, and plant height could also be used as a selection criterion in breeding drought-tolerant rice due to high values of broad-sense heritability and genetic advance by percentage of mean (GAM). Nevertheless, further study on the genetics and physiological basis of tolerant ability at reproductive growth stages are necessary in order to assess grain yield potential of the potentially tolerant genotype reported in this study.

scholarly journals Improvement of drought tolerance in rice using line X tester mating design and biochemical molecular markers

2022 ◽  
Vol 46 (1) ◽  
Author(s):  
Almoataz Bellah Ali El-Mouhamady ◽  
Abdul Aziz M. Gad ◽  
Ghada S. A. Abdel Karim
Keyword(s):  
Water Stress ◽  
Drought Stress ◽  
Drought Tolerance ◽  
Stress Tolerance ◽  
Water Deficit ◽  
Scientific Progress ◽  
Crop Productivity ◽  
Hybrid Generation ◽  
Water Stress Tolerance ◽  
Rice Genotypes

Abstract Background Water stress, specifically the limited water resources needed to grow strategic crops, especially rice, poses a great threat to crop productivity. So, it was imperative that scientists all work together to try genetically improving the rice for drought tolerance in light of these environmental challenges. The aim of this study is trying to know the genetic behavior responsible for water-deficit tolerance in rice genotypes but at the molecular level. Moreover, this attempt will be an important leap in the process of genetic improvement in rice for water stress tolerance in Egypt. Results Twenty-three rice genotypes including eight parents and their fifteen F1 crosses or (the first hybrid generation) by line X tester analysis were evaluated for water stress tolerance during two experiments (the control and drought experiment) besides some molecular–biochemical studies for eight parents and the highest selected five crosses for water stress tolerance. The research revealed that five rice crosses out of fifteen hybrids were highly tolerant to water stress compared to the normal conditions. Data of biochemical markers indicated the presence of bands that are considered as molecular genetic markers for water-deficit tolerance in some rice genotypes, and this is the scientific progress achieved in this research. This was evident by increasing the density and concentration of SDS-protein electrophoresis besides enhancing the activities of peroxidase (POD) and polyphenol oxidase (PPO) under water-deficit conditions, which confirmed the tolerance of drought stress in the eight rice genotypes and the best five crosses from the first hybrid generation. Conclusion The five promising and superior rice hybrids showed an unparalleled tolerance to water stress in all evaluated traits under water stress treatment compared to the standard experiment. Also, biochemical and molecular parameters evidence confirmed the existence of unquestionable evidence that it represents the main nucleus for producing rice lines tolerated for drought stress under Egyptian conditions.

scholarly journals Validation of Drought Tolerance Gene-linked Microsatellite Markers and Their Efficiency for Diversity Assessment in a Set of Soybean Genotypes

2021 ◽  
pp. 48-57
Author(s):  
Nishi Mishra ◽  
M. K. Tripathi ◽  
Niraj Tripathi ◽  
Sushma Tiwari ◽  
Neha Gupta ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Drought Tolerance ◽  
Ssr Markers ◽  
Diversity Analysis ◽  
Molecular Screening ◽  
Genetic Diversity Analysis ◽  
Drought Tolerant ◽  
Diversity Assessment ◽  
Soybean Genotypes ◽  
Tolerance Gene

Aim: Soybean is well-thought-out to be a major crop owing to its significant involvement as vegetable oil and protein in human diet. However, inopportunely, its production has been melodramatically declined attributable to the commonness of drought related stress. Study Design: During the present study a total of 53 soybean genotypes were selected. For molecular diversity analysis as well as validation total 12 SSR markers were used. Molecular screening of soybean genotypes was done to determine the efficiency of available markers in genetic diversity analysis as well as their validation on the basis of their association with drought tolerance gene. Place and Duration of the Study: The present study was conducted at Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Gwalior, Rajmata Vijayaraje Scindia Krishi Vishwa Vidyalaya, Gwalior, M.P., India during the year 2018 - 2019. Methodology: Template DNA of all 53 selected soybean genotypes extracted for molecular screening. The current investigation has been accomplished to validate the available SSR markers with their efficiency in genetic diversity analysis in a set of soybean genotypes. Results: Among applied drought tolerance gene-linked 12 SSR molecular markers, the highest genetic diversity (0.6629) was noticed in Satt520 while lowest (0.0370) was in Satt557 with an average of 0. 3746.While, the highest PIC value was 0.5887 prearranged by Satt520 and lowest 0.0363 by Satt557 with the mean worth of 0.3063. Conclusion: Dendrogram constructed on the basis of banding profile of employed markers was able to discriminate some putative drought tolerant genotypes i.e., JS97-52, JS95-60 from rest of the genotypes. The results of the present examination may donate towards enhancement of soybean genotypes to bread drought tolerant varieties.

scholarly journals Growth Performance and Antioxidant Enzyme Activities of Advanced Mutant Rice Genotypes under Drought Stress Condition

Agronomy ◽  
2018 ◽  
Vol 8 (12) ◽  
pp. 279 ◽  
Author(s):  
Zarifth Kamarudin ◽  
Mohd Yusop ◽  
Mahmud Tengku Muda Mohamed ◽  
Mohd Ismail ◽  
Abdul Harun
Keyword(s):  
Drought Stress ◽  
Drought Tolerance ◽  
Enzyme Activities ◽  
Stress Condition ◽  
Growth Parameters ◽  
Proline Content ◽  
Antioxidant Enzyme Activities ◽  
Drought Tolerant ◽  
Drought Stress Condition ◽  
Rice Genotypes

Drought stress affects various physiological and metabolic processes in rice (Oryza sativa L.) plant. Non-availability of high-yielding varieties suitable for cultivation under drought condition lead towards a sharp decline in rice yield. Induce mutation is an essential auxiliary approach to counterpart conventional breeding to produce stress-tolerance rice variety. The current study was aimed to identify two advanced mutant rice genotypes as drought-tolerant using growth parameters and antioxidant enzyme activities. The advanced mutant rice genotypes, MR219-4 and MR219-9, showed a minimal reduction on all growth parameters, yield, and yield components measured for drought tolerance. MR219-4 had a slight reduction on total dry weight and chlorophyll content under drought stress condition. Proline content increased significantly in drought-tolerant rice genotypes and the highest proline content was obtained from MR219-4 followed by MR219-9 under drought stress. Catalase, ascorbate peroxidase, and guaiacol peroxidase activities were significantly increased in drought stress treatment in all the rice genotypes. MR219-4 and MR219-9 were identified as high-yielding drought-tolerant genotypes as they maintained good performance under drought stress condition for all the measured traits compared to the drought-tolerant check varieties, Aeron1 and MR219, thus, this might be underlying selection criteria for a drought tolerance rice breeding programme.

scholarly journals Potentiality of Some Egyptan Cotton Varieties under Drought Stress Conditions

2019 ◽  
pp. 1-7
Author(s):  
M. D. H. Dewdar
Keyword(s):  
Water Stress ◽  
Drought Stress ◽  
Yield Components ◽  
Block Design ◽  
Stress Conditions ◽  
Yield Potential ◽  
High Yield ◽  
Stress Treatment ◽  
Irrigation Regimes ◽  
Yield And Yield Components

This study aimed to investigate the performance of three cotton (Gossypium barbadense L.) genotypes as affected by drought stress at three irrigation regimes; 14 (S-0), 21(S-1) and 28 (S-2) days that were started after the first irrigation. To achieve this goal, a field experiment was conducted as split block design at the Experimental Farm of the Faculty of Agriculture, El-Fayoum Univ. The results indicated that the irrigation regimes mean squares of combined data were highly significant for earliness traits, also as well as yield and yield components.  Most of fiber properties were not affected by water stress conditions. Significant differences were found among the non- stress (S-0) and the stress treatments (S-1 and S-2) for mean performances of the three earliness traits. Treatment S-2 led to significant decrease in yield and yield components compared to S-0. The results showed that Giza 85 variety gave the highest fiber length, fiber strength and was finer cultivars having the lower micronaire values. The interaction between genotypes and stress treatments was significant for most traits.G1,G2 and G3 cotton varieties  exhibited highest seed cotton yield kg ha-1 (yield potential) in the non- stress treatment (S-0).  Giza 90 variety outyielded the other two varieties under stress treatment (S-2) compared to those of Giza 85 and Giza 83. The superiority of Giza 90 variety could be attributed to its high yield components., while Giza 90 was relatively stress susceptible and similar trend of those obtained using data of relative productivity (%) which confirm that the genotype Giza 83 and Giza 85 are more drought tolerance and could be used as sources of drought stress tolerance in breeding programs and tolerance to water stress conditions.

scholarly journals Effect of Watering Regimes on Yield and Agronomic Traits of Exotic Groundnut Genotypes in Tanzania

2019 ◽  
pp. 1-6
Author(s):  
Mashamba Philipo ◽  
Susan Nchimbi-Msolla
Keyword(s):  
Water Stress ◽  
Drought Tolerance ◽  
Plant Height ◽  
Agronomic Traits ◽  
Stress Conditions ◽  
Pod Yield ◽  
Drought Tolerant ◽  
Arachis Hypogaea L ◽  
Screen House ◽  
Plot Design

Groundnut (Arachis hypogaea L.) production in rain fed regions of Africa is mostly affected by intermittent drought of different duration and intensity. Improvement of groundnuts for drought tolerance could increase production in drought prone areas. Therefore, this study aimed at (i) determining the effect of Watering regimes on yield and agronomic traits of exotic Groundnut genotypes and (ii) identifying drought tolerant genotypes as source material for breeding and drought tolerant varieties. Thirty groundnut genotypes were evaluated for drought tolerance under well watered and water stress conditions in the screen house at Sokoine University of Agriculture (SUA), Tanzania. A split plot design with four replications was used whereby the watering regimes were the main plots with varieties planted as subplots Data were recorded on plant height, number of pod/plant and pod yield/plant. Results showed that drought significantly reduced pod yield, number of pods/plant and plant height. Eleven genotypes namely; ICG 2106, ICR 48, ICGS 44, ICG 3053, ICG 11088, ICGV-SM 87003, ICG 12235, ICG 13723, ICGV 02271, ICGV 97182 and ICGV 91114 gave better pod yield and number of pods/plants in water stress conditions and are recommended for use in breeding program as drought tolerant varieties and sources for breeding materials.

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