scholarly journals Ginkgo biloba L. Leaf Extract Protects HepG2 Cells Against Paraquat-Induced Oxidative DNA Damage

Plants ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 556 ◽  
Author(s):  
Amélia M. Silva ◽  
Sandra C. Silva ◽  
Jorge P. Soares ◽  
Carlos Martins-Gomes ◽  
João Paulo Teixeira ◽  
...  

Ginkgo biloba L. leaf extracts and herbal infusions are used worldwide due to the health benefits that are attributed to its use, including anti-neoplastic, anti-aging, neuro-protection, antioxidant and others. The aim of this study was to evaluate the effect of an aqueous Ginkgo biloba extract on HepG2 cell viability, genotoxicity and DNA protection against paraquat-induced oxidative damage. Exposure to paraquat (PQ), over 24 h incubation at 1.0 and 1.5 µM, did not significantly reduce cell viability but induced concentration and time-dependent oxidative DNA damage. Ginkgo biloba leaf extract produced dose-dependent cytotoxicity (IC50 = 540.8 ± 40.5 µg/mL at 24 h exposure), and short incubations (1 h) produced basal and oxidative DNA damage (>750 and 1500 µg/mL, respectively). However, lower concentrations (e.g., 75 µg/mL) of Ginkgo biloba leaf extract were not cytotoxic and reduced basal DNA damage, indicating a protective effect at incubations up to 4 h. On the other hand, longer incubations (24 h) induced oxidative DNA damage. Co-incubation of HepG2 cells for 4 h, with G. biloba leaf extract (75 µg/mL) and PQ (1.0 or 1.5 µM) significantly reduced PQ-induced oxidative DNA damage. In conclusion, the consumption of Ginkgo biloba leaf extract for long periods at high doses/concentrations is potentially toxic; however, low doses protect the cells against basal oxidative damage and against environmentally derived toxicants that induce oxidative DNA damage.

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Zhuhong Zhang ◽  
Si Chen ◽  
Hu Mei ◽  
Jiekun Xuan ◽  
Xiaoqing Guo ◽  
...  

2008 ◽  
Vol 202 (1-3) ◽  
pp. 187-196 ◽  
Author(s):  
Yi-Ling Yang ◽  
Chia-Wen Hsieh ◽  
Yu-Yuan P. Wo ◽  
Yi-Chung Yang ◽  
Kwok-Tung Lu

2014 ◽  
Vol 139 (2) ◽  
pp. 338-349 ◽  
Author(s):  
Haixia Lin ◽  
Xiaoqing Guo ◽  
Suhui Zhang ◽  
Stacey L. Dial ◽  
Lei Guo ◽  
...  

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