scholarly journals Graphene Oxide Nanoparticles Modified Paper Electrode as a Biosensing Platform for Detection of the htrA Gene of O. tsutsugamushi

Sensors ◽  
2021 ◽  
Vol 21 (13) ◽  
pp. 4366
Author(s):  
Deepak Kala ◽  
Tarun Kumar Sharma ◽  
Shagun Gupta ◽  
Vivek Verma ◽  
Atul Thakur ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Electrochemical Properties ◽  
Differential Pulse Voltammetry ◽  
Electrochemical Impedance ◽  
Low Cost ◽  
Differential Pulse ◽  
Oxide Nanoparticles ◽  
Layer By Layer ◽  
Dna Sensor ◽  
Pulse Voltammetry

The unique structural and electrochemical properties of graphene oxide (GO) make it an ideal material for the fabrication of biosensing devices. Therefore, in the present study, graphene oxide nanoparticles modified paper electrodes were used as a low-cost matrix for the development of an amperometric DNA sensor. The graphene oxide was synthesized using the modified hummers method and drop cast on a screen-printed paper electrode (SPPE) to enhance its electrochemical properties. Further, the GO/SPPE electrode was modified with a 5′NH2 labeled ssDNA probe specific to the htrA gene of Orientia tsutsugamushi using carbodiimide cross-linking chemistry. The synthesized GO was characterized using UV-Vis, FTIR, and XRD. The layer-by-layer modification of the paper electrode was monitored via FE-SEM, cyclic voltammetry, and electrochemical impedance spectroscopy (EIS). The sensor response after hybridization with single-stranded genomic DNA (ssGDNA) of O. tsutsugamushi was recorded using differential pulse voltammetry (DPV). Methylene blue (1 mM in PBS buffer, pH 7.2) was used as a hybridization indicator and [Fe(CN)6]−3/−4 (2.5 mM in PBS buffer, pH 7.2) as a redox probe during electrochemical measurements. The developed DNA sensor shows excellent sensitivity (1228.4 µA/cm2/ng) and LOD (20 pg/µL) for detection of O. tsutsugamushi GDNA using differential pulse voltammetry (DPV).

scholarly journals Adsorption of Guanine, Guanosine, and Adenine at Electrodes Studied by Differential Pulse Voltammetry and Electrochemical Impedance

Langmuir ◽  
2002 ◽  
Vol 18 (6) ◽  
pp. 2326-2330 ◽  
Author(s):  
Ana Maria Oliveira-Brett ◽  
Luís Antônio da Silva ◽  
Christopher M. A. Brett
Keyword(s):  
Differential Pulse Voltammetry ◽  
Electrochemical Impedance ◽  
Differential Pulse ◽  
Pulse Voltammetry

Simultaneous determination of Pb and Cd in low-cost jewelry using differential pulse voltammetry

2016 ◽  
Vol 8 (45) ◽  
pp. 8028-8032
Author(s):  
Taimara Polidoro Ferreira ◽  
Rafael Arromba de Sousa ◽  
Denise Lowinsohn
Keyword(s):  
Differential Pulse Voltammetry ◽  
Simultaneous Determination ◽  
Low Cost ◽  
Differential Pulse ◽  
Electrochemical Determination ◽  
Pulse Voltammetry

The simultaneous electrochemical determination of lead (Pb) and cadmium (Cd) in low-cost jewelry was achieved using differential pulse voltammetry (DPV).

scholarly journals Determination of Methyldopa and Paracetamol in Pharmaceutical Samples by a Low Cost Genipa americana L. Polyphenol Oxidase Based Biosensor

2019 ◽  
Vol 9 (3) ◽  
pp. 416-422
Author(s):  
Rafael Souza Antunes ◽  
Douglas Vieira Thomaz ◽  
Luane Ferreira Garcia ◽  
Eric de Souza Gil ◽  
Vernon Sydwill Sommerset ◽  
...  
Keyword(s):  
Electrochemical Impedance ◽  
Low Cost ◽  
Differential Pulse ◽  
Pharmaceutical Samples ◽  
Polyphenol Oxidases ◽  
Voltammetric Techniques ◽  
Relative Standard ◽  
Pulse Voltammetry ◽  
Phenolic Drugs

Purpose: Jenipapo fruit (Genipa americana L) is a natural source of polyphenol oxidases (PPOs) whose potential in pharmaceutical analysis is noteworthy. Henceforth, this work reports the electrochemical study of a low-cost PPO-based biosensor produced from the crude extract of Jenipapo fruits and accounts a practical approach to employ this biosensor in the determination of methyldopa and paracetamol in pharmaceutical samples. Methods: In order to investigate the electrochemical properties of the biosensor, theoretical and practical approaches were employed, and both samples and the biosensor were analyzed through electrochemical impedance spectroscopy (EIS) and voltammetric techniques, namely: differential pulse voltammetry (DPV) and cyclic voltammetry (CV). Results: showcased that the biosensor presented good analytical features, as well as low detection limits (8 μmol L-1 for methyldopa and 5 μmol L-1 for paracetamol). The relative standard deviation was less than 5% mid-assay. Conclusion: The use of this biosensor is a reliable, low cost and useful alternative in the pharmaceutic determination of phenolic drugs (e.g. methyldopa and paracetamol).

scholarly journals CUPRAC Voltammetric Determination of Antioxidant Capacity in Tea Samples by Using Screen-Printed Microelectrodes

2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
Alina Elena Trofin ◽  
Lucia Carmen Trincă ◽  
Elena Ungureanu ◽  
Adina Mirela Ariton
Keyword(s):  
Antioxidant Capacity ◽  
Differential Pulse Voltammetry ◽  
Low Cost ◽  
Differential Pulse ◽  
Food Samples ◽  
Open Circuit ◽  
Rapid Response ◽  
Detection Methods ◽  
Pulse Voltammetry ◽  
Screen Printed

Measurement of antioxidant capacity represents an analytical major challenge in terms of accuracy, efficiency, rapid response, or low cost of detection methods. Quantification of antioxidant capacity of food samples using disposable screen‐printed microelectrodes (SPMEs) was based on cyclic voltammetry versus open-circuit potential (CV vs OCP) and differential pulse voltammetry (DPV) as compared with spectrophotometric measurement of the CUPRAC reaction with 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (trolox). The SPMEs are organic‐resistant electrodes and thus compatible with food samples and organic solvents used to dissolve trolox. A micropipette was used to release a drop of 50 μL sample on the spotted surface of the SPME sensor/working electrode that was time programmed to function according to the working protocol. The SPME response was linearly correlated with trolox content. This preliminary demonstration was focused on the analysis of tea infusions, due to the simplicity and reproducibility of the samples’ preparations involved. Analytical results of the antioxidant capacity (expressed as mol·L−1 trolox equivalents) of the tea samples showed a good agreement in the case of spectrophotometry and differential pulse voltammetry (R2 > 0.998). DPV with SPME based on CUPRAC reactions was proven to be a promising approach for the characterization of antioxidant capacity of tea samples with rapid response, cost-effectiveness, and simplicity of operation.

scholarly journals Recycling Chocolate Aluminum Wrapping Foil as to Create Electrochemical Metal Strip Electrodes

Molecules ◽  
2020 ◽  
Vol 26 (1) ◽  
pp. 21
Author(s):  
Hairul Hisham Hamzah ◽  
Nur Hidayah Saleh ◽  
Bhavik Anil Patel ◽  
Mohd Muzamir Mahat ◽  
Saiful Arifin Shafiee ◽  
...  
Keyword(s):  
Electrochemical Sensors ◽  
Electrochemical Impedance ◽  
Low Cost ◽  
Differential Pulse ◽  
Redox Species ◽  
X Ray ◽  
Conductive Materials ◽  
Electrode Surfaces ◽  
Electrical Conductivities ◽  
Pulse Voltammetry

The development of low-cost electrode devices from conductive materials has recently attracted considerable attention as a sustainable means to replace the existing commercially available electrodes. In this study, two different electrode surfaces (surfaces 1 and 2, denoted as S1 and S2) were fabricated from chocolate wrapping aluminum foils. Energy dispersive X-Ray (EDX) and field emission scanning electron microscopy (FESEM) were used to investigate the elemental composition and surface morphology of the prepared electrodes. Meanwhile, cyclic voltammetry (CV), chronoamperometry, electrochemical impedance spectroscopy (EIS), and differential pulse voltammetry (DPV) were used to assess the electrical conductivities and the electrochemical activities of the prepared electrodes. It was found that the fabricated electrode strips, particularly the S1 electrode, showed good electrochemical responses and conductivity properties in phosphate buffer (PB) solutions. Interestingly, both of the electrodes can respond to the ruthenium hexamine (Ruhex) redox species. The fundamental results presented from this study indicate that this electrode material can be an inexpensive alternative for the electrode substrate. Overall, our findings indicate that electrodes made from chocolate wrapping materials have promise as electrochemical sensors and can be utilized in various applications.

scholarly journals Determination of Trace Lead and Cadmium in Decorative Material Using Disposable Screen-Printed Electrode Electrically Modified with Reduced Graphene Oxide/L-Cysteine/Bi-Film

Sensors ◽  
2020 ◽  
Vol 20 (5) ◽  
pp. 1322 ◽  
Author(s):  
Xiaopeng Hou ◽  
Benhai Xiong ◽  
Yue Wang ◽  
Li Wang ◽  
Hui Wang
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Electrochemical Impedance ◽  
Low Cost ◽  
Linear Sweep Voltammetry ◽  
Differential Pulse ◽  
Screen Printed Electrode ◽  
Lead And Cadmium ◽  
Reduced Graphene ◽  
Screen Printed

Cadmium (Cd) and lead (Pb) in decorative materials threaten human health. To determine the content of Cd(II) and Pb(II), a disposable screen-printed electrode (DSPE) electrically modified with reduced graphene oxide (rGO) and L-cysteine (LC) was fabricated, which was further electroplated with bismuth film (Bi/LC-rGO/DSPE) in situ. The electrochemical properties of this electrode were studied using cyclic voltammetry, electrochemical impedance spectroscopy, linear sweep voltammetry and differential pulse voltammetry. The results indicated that the Bi/LC-rGO/DSPE had excellent sensitivity, selectivity and stability with low cost and easy production. After optimizing the detection parameters, the linear range of the Bi/LC-rGO/DSPE was from 1.0 to 30.0 μg/L for Cd(II) and Pb(II), and the detection limits were 0.10 μg/L for Cd(II) and 0.08 μg/L for Pb(II). Finally, the Bi/LC-rGO/DSPE was applied to determine the concentrations of Cd(II) and Pb(II) in different decorative materials where the recoveries were in the range from 95.86% to 106.64%.

scholarly journals Development of a Carbon Paste Electrode Containing Benzo-15-Crown-5 for Trace Determination of the Uranyl Ion by Using a Voltammetric Technique

2009 ◽  
Vol 92 (1) ◽  
pp. 241-247 ◽  
Author(s):  
Sunil K Agrahari ◽  
Sangita D Kumar ◽  
Ashwini K Srivastava
Keyword(s):  
Detection Limit ◽  
Differential Pulse Voltammetry ◽  
Modified Electrode ◽  
Carbon Paste Electrode ◽  
Electrochemical Impedance ◽  
Differential Pulse ◽  
Carbon Paste ◽  
Pulse Voltammetry ◽  
Paste Electrode

Abstract The interaction of macrocyclic compounds like crown ethers and UO22+ has been studied by electrochemical methods. A modified carbon paste electrode incorporating benzo-15-crown-5 (B15C5) was used to evaluate the electron transfer reaction of UO22+ by cyclic voltammetry, differential pulse voltammetry, and electrochemical impedance spectroscopy. Electrochemical impedance studies showed that charge transfer resistance was less for the B15C5-modified electrode than for the plain carbon paste electrode (PCPE). On the basis of these observations, a UO22+-sensitive crown ether chemically modified electrode (CME) for trace analysis was fabricated and investigated in aqueous solutions. It was found that a 5 B15C5CME for UO22+ showed a better voltammetric response than did the PCPE. UO22+ could be quantified at sub-μg/mL levels by differential pulse voltammetry with a detection limit of 0.03 μg/mL. By differential pulse adsorptive stripping voltammetry, UO22+ could be quantified in the working range of 0.002-0.2 μg/mL, with a detection limit of 1.1 μg/L. Simultaneous determination of UO22+, Pb2+, and Cd2+ was possible. The method was successfully applied to the determination of UO22+ in synthetic, as well as real, samples; the results were found to be comparable to those obtained by inductively coupled plasma-atomic emission spectroscopy.

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