scholarly journals Cholesterol Reduction and Vitamin B12 Production Study on Enterococcus faecium and Lactobacillus pentosus Isolated from Yoghurt

2021 ◽  
Vol 13 (11) ◽  
pp. 5853
Author(s):  
Rajan A. Walhe ◽  
Sham S. Diwanay ◽  
Milind S. Patole ◽  
R. Z. Sayyed ◽  
Hind A. AL-Shwaiman ◽  
...  
Keyword(s):  
Vitamin B12 ◽  
Enterococcus Faecium ◽  
16S Rrna Gene Sequencing ◽  
Fermented Food ◽  
Bile Salt Hydrolase ◽  
Rrna Gene ◽  
Cholesterol Reduction ◽  
Lactobacillus Pentosus ◽  
Probiotic Potential ◽  
B12 Production

The present study was aimed to test cholesterol reduction and vitamin B12 production abilities of the isolated lactic acid bacteria (LAB). Three LAB isolates, namely, Enterococcus faecium (EF), Enterococcus faecium (Chole1), and Lactobacillus pentosus (7MP), having probiotic potential, were isolated from yoghurt. These isolates were screened for bile salt hydrolase (BSH) activity, cholesterol reduction property in MRS broth, and the production of vitamin B12. The present study revealed that the isolate 7MP possesses the highest potential of (48%) cholesterol reduction compared to the other isolates. The isolates EF and Chole1 produced a good amount of (1 ng/mL) vitamin B12. These isolates were identified by 16S rRNA gene sequencing and confirmed by MALD_TOF analysis. Thus, the use of these LAB isolates for yoghurt-making can offer the value addition of lowering cholesterol and vitamin B12 fortification in fermented food.

scholarly journals Probiotic Potential and Cholesterol-Lowering Capabilities of Bacterial Strains Isolated from Pericarpium Citri Reticulatae ‘Chachiensis’

2021 ◽  
Vol 9 (6) ◽  
pp. 1224
Author(s):  
Qianxian He ◽  
Jingyu Li ◽  
Yongkai Ma ◽  
Qi Chen ◽  
Gu Chen
Keyword(s):  
16S Rrna Gene Sequencing ◽  
Fermented Food ◽  
Bile Salt Hydrolase ◽  
Rrna Gene ◽  
Probiotic Properties ◽  
Bacterial Strains ◽  
Cholesterol Removal ◽  
Carbohydrate Utilization ◽  
Cholesterol Lowering ◽  
Probiotic Potential

Pericarpium Citri Reticulatae ‘Chachiensis’ (PCR-Chachiensis), the pericarps of Citri Reticulatae Blanco cv. Chachiensis, is a food condiment and traditional medicine in southeast and eastern Asia. Its rich and various bacterial community awaits exploration. The present study is the first report on probiotic screening and characterization of bacteria from PCR-Chachiensis. Based on 64 culturable bacterial isolates, 8 strains were screened out to have great survival in the simulated gastrointestinal stressful condition, being nonhemolytic and without biogenic amine formation. They were identified by 16S rRNA gene sequencing as two Bacillus, three Lactobacillus, and three strains from Bacillales. Their probiotic properties, cholesterol-lowering potential and carbohydrate utilization capability were further investigated. Though these eight strains all displayed distinct cholesterol removal potential, Bacillus licheniformis N17-02 showed both remarkable cholesterol removal capability and presence of bile salt hydrolase gene, as well as possessing most of the desirable probiotic attributes. Thus, it could be a good probiotic candidate with hypocholesterolemic potential. Bacillus megaterium N17-12 displayed the widest carbohydrate utilization profile and the strongest antimicrobial activity. Hence, it was promising to be used as a probiotic in a host and as a fermentation starter in fermented food or feed.

Occurrence of bacteria with technological and probiotic potential in Argentinian human breast-milk

2020 ◽  
pp. 1-18 ◽  
Author(s):  
S. Oddi ◽  
A. Binetti ◽  
P. Burns ◽  
A. Cuatrin ◽  
J. Reinheimer ◽  
...  
Keyword(s):  
Breast Milk ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Human Breast Milk ◽  
Raw 264.7 Macrophages ◽  
Probiotic Potential ◽  
Long Term Storage ◽  
Technological Potential ◽  
Rrna Gene Sequencing

Breast milk can be a source of potential probiotic bacteria, but the technological capacity of isolates obtained from this source is not always guaranteed. We aimed at isolating lactobacilli from breast milk samples collected in Argentina, focusing on isolates with functional and technological potential as probiotics. Fourteen Lactobacillus and one Bifidobacterium isolates were obtained from 164 samples donated by 104 mothers. The isolates preliminarily identified by MALDI-TOF, and then the identity was confirmed by partial 16S rRNA gene sequencing. Hydrophobicity was determined (hexadecane and xylene partition). The strains were also co-cultured with murine RAW 264.7 macrophages for screening the capacity to induce the anti-inflammatory cytokine interleukin (IL)-10. Hydrophobicity ranged from 7.4 and 95.9%. The strains Lactobacillus gasseri (70a and 70c) and Lactobacillus plantarum (73a and 73b) were the strains with a higher capacity to induce IL-10 production by macrophages. The technological application was evaluated by freezing dried in 10% lactose or 10% polydextrose. The survival was assessed after accelerated (37 °C, 4 weeks) or long-term (5 and 25 °C, 12 months) storage. Except for Lactobacillus gallinarum 94d, strains lost less than 1 Log10 order cfu/g after long-term (12 months) storage at 5 °C in lactose and polydextrose as protectants. A low correlation between survival to accelerated and long-term storage tests was observed. L. gasseri (70a and 70c) and L. plantarum (73a and 73b) deserve further studies as potential probiotics due to their capacity to induce IL-10 from murine macrophages and their hydrophobicity. In special, L. plantarum 73a was able to confer enhanced protection against Salmonella infection by promoting the immunity of the small intestine.

scholarly journals Microbe-Metabolite Associations Linked to the Rebounding Murine Gut Microbiome Postcolonization with Vancomycin-Resistant Enterococcus faecium

mSystems ◽  
2020 ◽  
Vol 5 (4) ◽  
Author(s):  
Andre Mu ◽  
Glen P. Carter ◽  
Lucy Li ◽  
Nicole S. Isles ◽  
Alison F. Vrbanac ◽  
...  
Keyword(s):  
Gut Microbiome ◽  
Enterococcus Faecium ◽  
Molecular Mechanisms ◽  
Microbial Community Composition ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Content Type ◽  
Functional Roles ◽  
Specific Pathogen ◽  
Vancomycin Resistant

ABSTRACT Vancomycin-resistant Enterococcus faecium (VREfm) is an emerging antibiotic-resistant pathogen. Strain-level investigations are beginning to reveal the molecular mechanisms used by VREfm to colonize regions of the human bowel. However, the role of commensal bacteria during VREfm colonization, in particular following antibiotic treatment, remains largely unknown. We employed amplicon 16S rRNA gene sequencing and metabolomics in a murine model system to try and investigate functional roles of the gut microbiome during VREfm colonization. First-order taxonomic shifts between Bacteroidetes and Tenericutes within the gut microbial community composition were detected both in response to pretreatment using ceftriaxone and to subsequent VREfm challenge. Using neural networking approaches to find cooccurrence profiles of bacteria and metabolites, we detected key metabolome features associated with butyric acid during and after VREfm colonization. These metabolite features were associated with Bacteroides, indicative of a transition toward a preantibiotic naive microbiome. This study shows the impacts of antibiotics on the gut ecosystem and the progression of the microbiome in response to colonization with VREfm. Our results offer insights toward identifying potential nonantibiotic alternatives to eliminate VREfm through metabolic reengineering to preferentially select for Bacteroides. IMPORTANCE This study demonstrates the importance and power of linking bacterial composition profiling with metabolomics to find the interactions between commensal gut bacteria and a specific pathogen. Knowledge from this research will inform gut microbiome engineering strategies, with the aim of translating observations from animal models to human-relevant therapeutic applications.

Lactococcus formosensis sp. nov., a lactic acid bacterium isolated from yan-tsai-shin (fermented broccoli stems)

2014 ◽  
Vol 64 (Pt_1) ◽  
pp. 146-151 ◽  
Author(s):  
Yi-sheng Chen ◽  
Misa Otoguro ◽  
Yu-hsuan Lin ◽  
Shwu-fen Pan ◽  
Si-hua Ji ◽  
...  
Keyword(s):  
Type Strain ◽  
Type Species ◽  
Sequence Similarity ◽  
16S Rrna Gene Sequencing ◽  
Housekeeping Genes ◽  
Fermented Food ◽  
Rrna Gene ◽  
Content Type ◽  
Lactococcus Garvieae ◽  
Link Type

A coccal-shaped organism, designated 516T, was isolated from yan-tsai-shin (fermented broccoli stems), a traditional fermented food in Taiwan. 16S rRNA gene sequencing results showed that strain 516T had 98.9 % sequence similarity to that of the type strain Lactococcus garvieae NBRC 100934T. Comparison of three housekeeping genes, rpoA, rpoB and pheS, revealed that strain 516T was well separated from Lactococcus garvieae NBRC 100934T. DNA–DNA hybridization studies indicated that strain 516T had low DNA relatedness with Lactococcus garvieae NBRC 100934T (46.1 %). The DNA G+C content of strain 516T was 38.1 mol% and the major fatty acids were C16 : 0 (22.7 %), C19 : 0 cyclo ω8c (17.9 %) and summed feature 7 (29.0 %). Based on the evidence, strain 516T represents a novel species of the genus Lactococcus , for which the name Lactococcus formosensis sp. nov. is proposed. The type strain is 516T ( = NBRC 109475T = BCRC 80576T).

scholarly journals Diversity of lactic acid bacteria isolated during fermentation of indigenous cassava obtained from Sumba, East Nusa Tenggara, Indonesia

2021 ◽  
Vol 22 (7) ◽  
Author(s):  
IMMANUELLA BERLIANA ELVANI HUTAJULU ◽  
PERISKILA DINA KALI KULLA ◽  
Endah Retnaningrum
Keyword(s):  
Enzyme Activity ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Animal Feed ◽  
16S Rrna Gene Sequencing ◽  
Fermented Food ◽  
Rrna Gene ◽  
Acid Fermentation ◽  
Study Results ◽  
Food And Beverage

Abstract. Hutajulu IBE, Kulla PDK, Retnaningrum E. 2021. Diversity of lactic acid bacteria isolated during fermentation of indigenous cassava obtained from Sumba, East Nusa Tenggara, Indonesia. Biodiversitas 22: 2561-2570. Traditional fermented food products hold a great significance in the routine diet of Indonesian inhabitants. The present study investigated the diversity of lactic acid bacteria (LAB) found in fermented indigenous cassava, ubi karet busuk, produced in Sumba, East Nusa Tenggara, Indonesia. The isolated LAB was characterized for their phenotypic, biochemical, and molecular traits, which involved 16S rRNA gene sequencing, determination of lactic acid fermentation pathway, and measurement of b-glucosidase and tannase activity in the LAB strains. During fermentation, 15 LAB strains were obtained from the cassava. Among these, 93.33% strains were found to be homofermentative, whereas 6.73% strains displayed heterofermentative traits. BLASTn and phylogenetic analysis classified 15 LAB strains into six species, namely Enterococcus faecium, Enterococcus faecalis, Enterococcus casseliflavus, Leuconostoc mesenteroides, Lactococcus lactis, and Enterococcus sp. Among these, 10 LAB strains displayed b-glucosidase activity, with highest enzyme activity of 7.13 U/mL recorded in L. lactis F14IS3. Interestingly, two of these strains also showed expression of tannase, with F6IS1 strain displaying highest enzyme activity of 38.23 U/mL. Thus, the study results highlighted the suitability of these b-glucosidase and tannase-producing LAB strains to be used as a starter in the fermentation of cassava and other substrates such as vegetables, fruits, and legumes. These strains can also be utilized in animal feed, food, and beverage industries.

scholarly journals Microbe-metabolite associations linked to the rebounding murine gut microbiome post-colonization with vancomycin resistant Enterococcus faecium

2019 ◽  
Author(s):  
Andre Mu ◽  
Glen P. Carter ◽  
Lucy Li ◽  
Nicole S. Isles ◽  
Alison F. Vrbanac ◽  
...  
Keyword(s):  
Gut Microbiome ◽  
Enterococcus Faecium ◽  
Molecular Mechanisms ◽  
Microbial Community Composition ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Functional Roles ◽  
Specific Pathogen ◽  
Vancomycin Resistant ◽  
Rrna Gene Sequencing

AbstractVancomycin resistant Enterococcus faecium (VREfm) is an emerging antibiotic resistant pathogen. Strain-level investigations are beginning to reveal the molecular mechanisms used by VREfm to colonize regions of the human bowel. However, the role of commensal bacteria during VREfm colonization, in particular following antibiotic treatment, remains largely unknown. We employed amplicon 16S rRNA gene sequencing and metabolomics in a murine model system to try and investigate functional roles of the gut microbiome during VREfm colonization. First-order taxonomic shifts between Bacteroidetes and Tenerricutes within the gut microbial community composition were detected both in response to pretreatment using ceftriaxone, and to subsequent VREfm challenge. Using neural networking approaches to find co-occurrence profiles of bacteria and metabolites, we detected key metabolome features associated with butyric acid during and after VREfm colonization. These metabolite features were associated with Bacteroides, indicative of a transition towards a pre-antibiotic naïve microbiome. This study shows the impacts of antibiotics on the gut ecosystem, and the progression of the microbiome in response to colonisation with VREfm. Our results offer insights towards identifying potential non-antibiotic alternatives to eliminate VREfm through metabolic re-engineering to preferentially select for Bacteroides.ImportanceThis study demonstrates the importance and power of linking bacterial composition profiling with metabolomics to find the interactions between commensal gut bacteria and a specific pathogen. Knowledge from this research will inform gut microbiome engineering strategies, with the aim of translating observations from animal models to human-relevant therapeutic applications.

Molecular Identification of Lactic acid bacteria Isolated from Lactating Cattle Lower Gut as a Potential Probiotic Species

2020 ◽  
Author(s):  
K Habib ◽  
T Ahmad ◽  
A Riaz ◽  
G M Ali ◽  
I Arif ◽  
...  
Keyword(s):  
Molecular Identification ◽  
Bacterial Species ◽  
16S Rrna Gene Sequencing ◽  
Haemolytic Activity ◽  
Rrna Gene ◽  
Bacterial Strains ◽  
Pediococcus Pentosaceus ◽  
Balanced Diet ◽  
Probiotic Potential ◽  
Livestock Productivity

Balanced diet is a basic factor in livestock productivity. There is still insufficient knowledge on indigenous probiotic species of livestock gastrointestinal tract. The present experiment was conducted for isolation and molecular identification of probiotic bacterial species from cattle lower gut and to determine their probiotic potential. Twenty (20) bacterial isolates were identified morphological and biochemical methods. Out of twenty isolates, three strains (PBC-3, PBC-6 and PBC-9) were selected as presumptive probiotic strains based on their enzymatic potential (cellulolytic, amylolytic and proteolytic ability), survival ability in low pH, better hydrophobicity, auto-aggregation, gamma haemolytic activity and better results against antimicrobial species. These isolates were identified as Enterococcus faecalis (PBC-3), Pediococcus acidilacticii (PBC-6) and Pediococcus pentosaceus (PBC-9) by 16S rRNA gene sequencing. Results of present study illustrated that Pediococcus acidilacticii (PBC-6) and Pediococcus pentosaceus (PBC-9) bacterial strains showed best probiotic potential and can be used in cattle feed in future

scholarly journals Evaluation of the Antibacterial Activity and Probiotic Potential of Lactobacillus plantarum Isolated from Chinese Homemade Pickles

2020 ◽  
Vol 2020 ◽  
pp. 1-11
Author(s):  
Y. Zeng ◽  
Y. Li ◽  
Q. P. Wu ◽  
J. M. Zhang ◽  
X. Q. Xie ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Lactic Acid ◽  
Lactobacillus Plantarum ◽  
Antibiotic Resistance Genes ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Bile Salt Hydrolase ◽  
Rrna Gene ◽  
Probiotic Potential

This study investigated the antipathogenic activity and probiotic potential of indigenous lactic acid bacteria (LAB) isolated from Chinese homemade pickles. In total, 27 samples were collected from different sites in China. Fifty-nine yielded pure colonies were identified by 16S rRNA gene sequencing as LAB and were initially evaluated for the antibacterial activity in vitro. Initial screening yielded Lactobacillus plantarum GS083, GS086, and GS090, which showed a broad-spectrum antibacterial activity against food-borne pathogens, especially multidrug-resistant pathogens. Meanwhile, organic acids were mainly responsible for the antimicrobial activity of the LAB strains, and the most abundant of these was lactic acid (19.32 ± 0.95 to 24.79 ± 0.40 g/l). Additionally, three L. plantarum strains demonstrated several basic probiotic characteristics including cell surface hydrophobicity, autoaggregation, and survival under gastrointestinal (GI) tract conditions. The safety of these isolates was also evaluated based on their antibiotic susceptibility, hemolytic risk, bile salt hydrolase activity, and existence of virulence or antibiotic resistance genes. All strains were safe at both the genomic and phenotypic levels. Therefore, L. plantarum GS083, GS086, and GS090 are fairly promising probiotic candidates and may be favorable for use as preservatives in the food industry.

Lactococcus taiwanensis sp. nov., a lactic acid bacterium isolated from fresh cummingcordia

2013 ◽  
Vol 63 (Pt_7) ◽  
pp. 2405-2409 ◽  
Author(s):  
Yi-sheng Chen ◽  
Chi-huan Chang ◽  
Shwu-fen Pan ◽  
Li-ting Wang ◽  
Yu-chung Chang ◽  
...  
Keyword(s):  
Lactococcus Lactis ◽  
Type Species ◽  
Sequence Similarity ◽  
16S Rrna Gene Sequencing ◽  
Housekeeping Genes ◽  
Fermented Food ◽  
Rrna Gene ◽  
Content Type ◽  
Link Type ◽  
Rrna Gene Sequencing

One coccal strain, designated 0905C15T, was isolated from fresh cummingcordia, which is the main ingredient of pobuzihi (fermented cummingcordia), a traditional fermented food in Taiwan. 16S rRNA gene sequencing results showed that strain 0905C15T had 98.22–98.82 % sequence similarity to that of the type strains of four Lactococcus lactis subspecies ( L. lactis subsp. lactis BCRC 12312T, L. lactis subsp. cremoris BCRC 12586T, L. lactis subsp. hordniae BCRC 80474T and L. lactis subsp. tructae BCRC 80475T). Comparison of two housekeeping genes, recA and rpoB, revealed that strain 0905C15T was well separated from the reference strains of the genus Lactococcus . DNA–DNA hybridization studies indicated that strain 0905C15T had low DNA relatedness to the four Lactococcus lactis subspecies (9.7–15.24 %). The DNA G+C content of strain 0905C15T was 39.6 mol %. Based on the evidence, strain 0905C15T represents a novel species of the genus Lactococcus , for which the name Lactococcus taiwanensis sp. nov. is proposed. The type strain is 0905C15T ( = NBRC 109049T = BCRC 80460T).

scholarly journals Molecular identification and in vitro evaluation of probiotic functional properties of some Egyptian lactic acid bacteria and yeasts

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Al-Shimaa Ibrahim Ahmed ◽  
Gihan Mohamed El Moghazy ◽  
Tarek Ragab Elsayed ◽  
Hanan Abdel Latif Goda ◽  
Galal Mahmoud Khalafalla
Keyword(s):  
Lactic Acid ◽  
Cell Surface Hydrophobicity ◽  
16S Rrna Gene Sequencing ◽  
Surface Hydrophobicity ◽  
Rrna Gene ◽  
E Coli ◽  
Probiotic Potential ◽  
Health Promoting ◽  
Rrna Gene Sequencing

Abstract Background The health-promoting effects along with global economic importance of consuming food products supplemented with probiotic microorganisms encouraged the researchers to discover new probiotics. Results Fourteen lactic acid bacterial isolates were identified as Enterococcus mediterraneensis, Lactobacillus fermentum, and Streptococcus lutetiensis by 16S rRNA gene sequencing, and in vitro characterized for their actual probiotic potential. All E. mediterraneensis isolates were resistant to clindamycin, whereas Lb. fermentum isolates were resistant to ampicillin, clindamycin, and vancomycin. The E. mediterraneensis and Lb. fermentum isolates displayed high overall digestive survival, ranged from 1.35 ± 0.06 to 32.73 ± 0.84% and from 2.01 ± 0.01 to 23.9 ± 1.85%, respectively. All isolates displayed cell surface hydrophobicity, ranged between 15.44 ± 6.72 and 39.79 ± 2.87%. The strongest auto-aggregation capability, higher than 40%, was observed for most E. mediterraneensis and Lb. fermentum isolates. The E. mediterraneensis isolates (L2, L12, and L15), Lb. fermentum (L8, L9, and L10), and Strep. lutetiensis (L14) exhibited the greatest co-aggregation with Salmonella typhimurium, Escherichia coli O157:H7, Staphylococcus aureus, and Bacillus cereus. Fifty-seven and fourteen hundredth percent of E. mediterraneensis isolates could be considered bacteriocinogenic against E. coli O157:H7, B. cereus, and S. aureus. Conclusion This study is the first one to isolate Enterococcus mediterraneensis in Egypt and to characterize it as new species of probiotics globally. According to the results, E. mediterraneensis (L2, L12, and L15), Lb. fermentum (L8, L9, and L10), and Strep. lutetiensis (L14) are the most promising in vitro probiotic candidates.

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