scholarly journals A Mouse Model of Oropharyngeal Papillomavirus-Induced Neoplasia Using Novel Tools for Infection and Nasal Anesthesia

Viruses ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 450 ◽  
Author(s):  
Andrea Bilger ◽  
Renee E. King ◽  
Josh P. Schroeder ◽  
Jared T. Piette ◽  
Louis A. Hinshaw ◽  
...  
Keyword(s):  
Mouse Model ◽  
Soft Palate ◽  
Human Head ◽  
Efficient System ◽  
Squamous Cells ◽  
Papillomavirus Infection ◽  
Induced Tumors ◽  
Nose Cone ◽  
Virus Delivery ◽  
Base Of The Tongue

Human head and neck cancers that develop from the squamous cells of the oropharynx (Oropharyngeal Squamous Cell Carcinomas or OPSCC) are commonly associated with the papillomavirus infection. A papillomavirus infection-based mouse model of oropharyngeal tumorigenesis would be valuable for studying the development and treatment of these tumors. We have developed an efficient system using the mouse papillomavirus (MmuPV1) to generate dysplastic oropharyngeal lesions, including tumors, in the soft palate and the base of the tongue of two immune-deficient strains of mice. To maximize efficiency and safety during infection and endoscopy, we have designed a nose cone for isoflurane-induced anesthesia that takes advantage of a mouse’s need to breathe nasally and has a large window for oral manipulations. To reach and infect the oropharynx efficiently, we have repurposed the Greer Pick allergy testing device as a virus delivery tool. We show that the Pick can be used to infect the epithelium of the soft palate and the base of the tongue of mice directly, without prior scarification. The ability to induce and track oropharyngeal papillomavirus-induced tumors in the mouse, easily and robustly, will facilitate the study of oropharyngeal tumorigenesis and potential treatments.

scholarly journals Tobacco mosaic virus delivery of mitoxantrone for cancer therapy

Nanoscale ◽  
2018 ◽  
Vol 10 (34) ◽  
pp. 16307-16313 ◽  
Author(s):  
Richard D. Lin ◽  
Nicole F. Steinmetz
Keyword(s):  
Breast Cancer ◽  
Cancer Therapy ◽  
Mouse Model ◽  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Triple Negative Breast Cancer ◽  
Topoisomerase Ii ◽  
Triple Negative ◽  
Topoisomerase Ii Inhibitor ◽  
Virus Delivery

Tobacco mosaic virus-nanoparticle encapsulation of the topoisomerase II inhibitor mitoxantrone enables therapy in a mouse model of triple negative breast cancer.

scholarly journals Association between human papillomavirus infection and atypical cervical squamous cells

2018 ◽  
Vol 71 (9-10) ◽  
pp. 301-308
Author(s):  
Drage Dabeski
Keyword(s):  
Human Papillomavirus ◽  
Squamous Cell ◽  
Cross Sectional Study ◽  
Histopathological Analysis ◽  
Cervical Lesions ◽  
Cross Sectional ◽  
Squamous Cells ◽  
Human Papillomavirus Infection ◽  
Papillomavirus Infection ◽  
Gynecology And Obstetrics

Introduction. The aim of the study was to confirm the association between human papillomavirus infection and atypical cervical squamous cells. Material and Methods. This cross-sectional study, conducted in the period from January 2016 to June 2017, included 128 sexually active women, aged 20 to 59 years with squamous cell abnormalities of the cervical cytology, who came to their annual gynecological exam at the University Clinic of Gynecology and Obstetrics in Skopje. All patients underwent human papillomavirus testing and colposcopic cervical biopsy with endocervical curettage for histopathological analysis. Results. Data analysis showed an increase in the human papillomavirus infection alongside with cytological (p = 0.029296) and histopathological (p = 0.029443) increasing grades of cervical lesions. It showed an association between the oncogenic potential of the virus and the cytological (p = 0.000086) and histopathological (p = 0.00001) grades of cervical lesions. A human papillomavirus infection was detected in 75.00% of the examined women. The relationship between the prevalence of high-risk and low-risk human papillomavirus genotypes was 56.25%: 10.94%. Mixed human papillomavirus infection was detected in 32.03% of all patients, in 42.71% of human papillomavirus positive patients. The most common human papillomavirus genotypes, in descending order, were human papillomavirus-16 (43.75%), human papillomavirus-31 (15.62%), human papillomavirus-18 10.4%), human papillomavirus-45 (9.37%), human papillomavirus-33 (7.29%), etc. Conclusion. This study has confirmed an association between human papillomavirus infection and squamous cell abnormalities of the uterine cervix. Young women under 30 years of age were the most affected group.

scholarly journals Garcinol sensitizes human head and neck carcinoma to cisplatin in a xenograft mouse model despite downregulation of proliferative biomarkers

Oncotarget ◽  
2015 ◽  
Vol 6 (7) ◽  
pp. 5147-5163 ◽  
Author(s):  
Feng Li ◽  
Muthu K. Shanmugam ◽  
Kodappully Sivaraman Siveen ◽  
Fan Wang ◽  
Tina H. Ong ◽  
...  
Keyword(s):  
Mouse Model ◽  
Head And Neck ◽  
Head And Neck Carcinoma ◽  
Human Head ◽  
Xenograft Mouse Model ◽  
Neck Carcinoma

scholarly journals Interaction between Chronic Inflammation and Oral HPV Infection in the Etiology of Head and Neck Cancers

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Mine Tezal
Keyword(s):  
Head And Neck ◽  
Chronic Inflammation ◽  
Potential Role ◽  
Hpv Infection ◽  
Oral Tongue ◽  
Papillomavirus Infection ◽  
Prevention And Treatment ◽  
Oral Hpv ◽  
Base Of The Tongue

Incidences of oral tongue, base of the tongue, and tonsil cancers have been increasing steadily in many parts of the world in spite of declining rates of tobacco use over the last four decades. A better understanding of the etiology, interactions between risk factors, and new approaches to prevention and treatment are necessary to change this course. This paper will present evidence supporting a potential role of chronic inflammation in the etiologies of oral human papillomavirus infection and head and neck squamous cell carcinoma, and it will discuss the implications for prevention and treatment.

scholarly journals Early Response Monitoring with 18F-FDG PET and Cetuximab-F(ab')2-SPECT After Radiotherapy of Human Head and Neck Squamous Cell Carcinomas in a Mouse Model

2014 ◽  
Vol 55 (10) ◽  
pp. 1665-1670 ◽  
Author(s):  
L. K. van Dijk ◽  
O. C. Boerman ◽  
G. M. Franssen ◽  
J. Lok ◽  
J. H. A. M. Kaanders ◽  
...  
Keyword(s):  
Mouse Model ◽  
Head And Neck ◽  
Squamous Cell ◽  
Fdg Pet ◽  
Human Head ◽  
Early Response ◽  
Squamous Cell Carcinomas ◽  
Response Monitoring ◽  
18F Fdg

A study of the relationship between nuclear contour thickening, nuclear enlargement and human papillomavirus infection in squamous cells

Cytopathology ◽  
2019 ◽  
Vol 30 (6) ◽  
pp. 644-649
Author(s):  
Junko Iijima ◽  
Kaori Okayama ◽  
Koji Teruya ◽  
Hiromi Hata ◽  
Natsuko Shiina ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Squamous Cells ◽  
Human Papillomavirus Infection ◽  
Papillomavirus Infection ◽  
The Relationship ◽  
Nuclear Enlargement

scholarly journals Changes in Pharyngeal Airway After Mandibular Setback Surgery: A Retrospective Cephalometric Study

2019 ◽  
Vol 53 (4) ◽  
pp. 256-263
Author(s):  
Jayshree D. Daryanani ◽  
N. Vijay ◽  
K. Sadashiva Shetty ◽  
Riddhi Chawla
Keyword(s):  
Soft Palate ◽  
Sagittal Split Ramus Osteotomy ◽  
Mandibular Prognathism ◽  
Pharyngeal Airway ◽  
Mandibular Setback ◽  
Mandibular Setback Surgery ◽  
Cephalometric Study ◽  
Base Of The Tongue

Aim: To cephalometrically evaluate the alterations taking place in the pharyngeal airway space, hyoid bone, soft palate, and head posture after mandibular setback surgery (bilateral sagittal split osteotomy) for correction of mandibular prognathism, as well as to evaluate the immediate postsurgical and more than 6 months postsurgical adaptations of these structures. Setting and Design: A retrospective cephalometric study. Materials and Method: The study group consisted of 40 nongrowing patients (20 females and 20 males) with mandibular prognathism and ANB less than or equal to –2 degree for which mandibular setback surgery by bilateral sagittal split ramus osteotomy was performed along with fixed appliance therapy. There presurgical (T1), immediate postsurgical (T2), and more than 6 months postsurgical (T3) lateral cephalograms were analyzed. Statistical Analysis Used: Intragroup comparison was done by paired t-test. Results: Skeletal measurements after orthognathic surgery remained stable in the long term. It was evident that mandibular setback surgery narrowed the pharyngeal airway; however, these changes were not significant except at the base of the tongue. During the follow-up airway measurements at the base of the tongue, the intersection of tongue at the inferior border of mandible and the vallecula increased but they did not reach the presurgical values. Soft palate length increased significantly after surgery. Angulation of soft palate to nasal line also increased significantly following surgery but decreased during follow-up. Extension of the head occurred after surgery which was maintained even at long term. Conclusion: Careful analysis of airway should be performed, particularly in connection with large anteroposterior discrepancies and in those who have risk factors for development of obstructive sleep apnea. Such cases should be corrected by combined maxillary and mandibular osteotomies.

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