scholarly journals A Replicating Viral Vector Greatly Enhances Accumulation of Helical Virus-Like Particles in Plants

Viruses ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 885
Author(s):  
Eva C. Thuenemann ◽  
Matthew J. Byrne ◽  
Hadrien Peyret ◽  
Keith Saunders ◽  
Roger Castells-Graells ◽  
...  
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Potato Virus ◽  
Viral Vector ◽  
Potato Virus X ◽  
Efficient Production ◽  
Coat Proteins ◽  
Rna Sequences ◽  
Virus Like Particles ◽  
Papaya Mosaic Virus

The production of plant helical virus-like particles (VLPs) via plant-based expression has been problematic with previous studies suggesting that an RNA scaffold may be necessary for their efficient production. To examine this, we compared the accumulation of VLPs from two potexviruses, papaya mosaic virus and alternanthera mosaic virus (AltMV), when the coat proteins were expressed from a replicating potato virus X- based vector (pEff) and a non-replicating vector (pEAQ-HT). Significantly greater quantities of VLPs could be purified when pEff was used. The pEff system was also very efficient at producing VLPs of helical viruses from different virus families. Examination of the RNA content of AltMV and tobacco mosaic virus VLPs produced from pEff revealed the presence of vector-derived RNA sequences, suggesting that the replicating RNA acts as a scaffold for VLP assembly. Cryo-EM analysis of the AltMV VLPs showed they had a structure very similar to that of authentic potexvirus particles. Thus, we conclude that vectors generating replicating forms of RNA, such as pEff, are very efficient for producing helical VLPs.

scholarly journals Tobacco mosaic virus (TMV) and potato virus X (PVX) coat proteins confer heterologous interference to PVX and TMV infection, respectively

2006 ◽  
Vol 87 (4) ◽  
pp. 1005-1012 ◽  
Author(s):  
A. A. Bazzini ◽  
S. Asurmendi ◽  
H. E. Hopp ◽  
R. N. Beachy
Keyword(s):  
Transgenic Plants ◽  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Potato Virus ◽  
Quaternary Structure ◽  
Systemic Infection ◽  
Potato Virus X ◽  
Coat Proteins ◽  
Local Cell ◽  
Cell Spread

Replication of Potato virus X (PVX) was reduced in transgenic protoplasts that accumulated wild-type coat protein (CPWT) of Tobacco mosaic virus (TMV) or a mutant CP, CPT42W, that produced highly ordered states of aggregation, including pseudovirions. This reaction is referred to as heterologous CP-mediated resistance. However, protoplasts expressing a CP mutant that abolished aggregation and did not produce pseudovirions, CPT28W, did not reduce PVX replication. Similarly, in transgenic tobacco plants producing TMV CPWT or CPT42W, there was a delay in local cell-to-cell spread of PVX infection that was not observed in CPT28W plants or in non-transgenic plants. The results suggest that the quaternary structure of the TMV CP regulates the mechanism(s) of heterologous CP-mediated resistance. Similarly, transgenic protoplasts that produced PVX CP conferred transient protection against infection by TMV RNA. Transgenic plants that accumulated PVX CP reduced the cell-to-cell spread of infection and resulted in a delay in systemic infection following inoculation with TMV or TMV RNA. Heterologous CP-mediated resistance was characterized by a brief delay in systemic infection, whilst homologous CP-mediated resistance conferred reduced or no systemic infection.

scholarly journals Involvement of the chloroplast gene ferredoxin 1 in multiple responses of Nicotiana benthamiana to Potato virus X infection

2019 ◽  
Vol 71 (6) ◽  
pp. 2142-2156 ◽  
Author(s):  
Xue Yang ◽  
Yuwen Lu ◽  
Fang Wang ◽  
Ying Chen ◽  
Yanzhen Tian ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Mosaic Virus ◽  
Potato Virus ◽  
Tobacco Rattle Virus ◽  
Potato Virus X ◽  
Tomato Mosaic Virus ◽  
Pcr Analysis ◽  
Coat Proteins ◽  
Virus Induced Gene Silencing ◽  
Callose Deposition

Abstract The chloroplast protein ferredoxin 1 (FD1), with roles in the chloroplast electron transport chain, is known to interact with the coat proteins (CPs) of Tomato mosaic virus and Cucumber mosaic virus. However, our understanding of the roles of FD1 in virus infection remains limited. Here, we report that the Potato virus X (PVX) p25 protein interacts with FD1, whose mRNA and protein levels are reduced by PVX infection or by transient expression of p25. Silencing of FD1 by Tobacco rattle virus-based virus-induced gene silencing (VIGS) promoted the local and systemic infection of plants by PVX. Use of a drop-and-see (DANS) assay and callose staining revealed that the permeability of plasmodesmata (PDs) was increased in FD1-silenced plants together with a consistently reduced level of PD callose deposition. After FD1 silencing, quantitative reverse transcription–real-time PCR (qRT–PCR) analysis and LC-MS revealed these plants to have a low accumulation of the phytohormones abscisic acid (ABA) and salicylic acid (SA), which contributed to the decreased callose deposition at PDs. Overexpression of FD1 in transgenic plants manifested resistance to PVX infection, but the contents of ABA and SA, and the PD callose deposition were not increased in transgenic plants. Overexpression of FD1 interfered with the RNA silencing suppressor function of p25. These results demonstrate that interfering with FD1 function causes abnormal plant hormone-mediated antiviral processes and thus enhances PVX infection.

Effect of Kinetin on Tobacco Mosaic Virus and Potato Virus X Replication in Leaves of Systemic Hosts

1977 ◽  
Vol 90 (4) ◽  
pp. 342-349 ◽  
Author(s):  
A. V. Reunov ◽  
G. D. Reunova ◽  
L. A. Vasilyeva ◽  
V. G. Reifman
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Potato Virus ◽  
Potato Virus X
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