scholarly journals Monoclonal Antibodies Targeting an Opisthorchis viverrini Extracellular Vesicle Tetraspanin Protect Hamsters against Challenge Infection

Vaccines ◽  
2021 ◽  
Vol 9 (7) ◽  
pp. 740
Author(s):  
Wuttipong Phumrattanaprapin ◽  
Mark Pearson ◽  
Darren Pickering ◽  
Bemnet Tedla ◽  
Michael Smout ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Bile Duct ◽  
Subunit Vaccine ◽  
Liver Fluke ◽  
Passive Immunization ◽  
Opisthorchis Viverrini ◽  
Human Bile ◽  
A Subunit ◽  
Fluke Infection ◽  
Liver Fluke Infection

Opisthorchis viverrini causes severe pathology in the bile ducts of infected human hosts, and chronic infection can culminate in bile duct cancer. The prevention of infection by vaccination would decrease opisthorchiasis-induced morbidity and mortality. The tetraspanin protein, Ov-TSP-2, is located on the membrane of secreted extracellular vesicles (EVs), and is a candidate antigen for inclusion in a subunit vaccine. To address the role of anti-Ov-TSP-2 antibodies in protection, we assessed the protective capacity of anti-Ov-TSP-2 monoclonal antibodies (mAbs) against opisthorchiasis. Two anti-TSP-2 IgM mAbs, 1D6 and 3F5, and an isotype control were passively transferred to hamsters, followed by parasite challenge one day later. Hamsters that received 3F5 had 74.5% fewer adult flukes and 67.4% fewer eggs per gram of feces compared to hamsters that received the control IgM. Both 1D6 and 3F5 (but not the control IgM) blocked the uptake of fluke EVs by human bile duct epithelial cells in vitro. This is the first report of passive immunization against human liver fluke infection, and the findings portend the feasibility of antibody-directed therapies for liver fluke infection, bolstering the selection of TSPs as components of a subunit vaccine for opisthorchiasis and fluke infections generally.

scholarly journals Discovering proteins for chemoprevention and chemotherapy by curcumin in liver fluke infection-induced bile duct cancer

PLoS ONE ◽  
2018 ◽  
Vol 13 (11) ◽  
pp. e0207405 ◽  
Author(s):  
Jarinya Khoontawad ◽  
Kitti Intuyod ◽  
Rucksak Rucksaken ◽  
Nuttanan Hongsrichan ◽  
Chawalit Pairojkul ◽  
...  
Keyword(s):  
Bile Duct ◽  
Bile Duct Cancer ◽  
Liver Fluke ◽  
Fluke Infection ◽  
Duct Cancer ◽  
Liver Fluke Infection

scholarly journals Research progress of cholangiocarcinoma induced by liver fluke infection

2018 ◽  
Vol 6 (4) ◽  
pp. 112-117
Author(s):  
Quan Liang
Keyword(s):  
Bile Duct ◽  
Liver Fluke ◽  
The Body ◽  
Research Progress ◽  
Digenetic Trematode ◽  
Human Beings ◽  
Fluke Infection ◽  
Secretory Products ◽  
Liver Fluke Infection

AbstractA liver fluke is a digenetic trematode parasitizing in the hepatic ducts of human beings or animals. Patients with liver fluke infection suffer from a series of hepatobiliary diseases. The prevalence of cholangiocarcinoma is significantly high in areas with a high incidence of clonorchiasis. A liver fluke is an important biocarcinogenic factor in the occurrence of cholangiocarcinoma. The secretory products of the body of this parasite and long-term mechanical stimulation induce continuous inflammation of the bile duct. Gene expression of the bile duct cells is imbalanced, leading to carcinogenesis of the bile duct. This article provides a summary of recent studies on the epidemiology, clinicopathology, and molecular biology of cholangiocarcinoma induced by liver fluke infection.

Parasite-associated morbidity: Liver fluke infection and bile duct cancer in Northeast Thailand

1994 ◽  
Vol 24 (6) ◽  
pp. 833-843 ◽  
Author(s):  
Paiboon Sithithaworn ◽  
Melissa R. Haswell-Elkins ◽  
Pisaln Mairiang ◽  
Soisungwan Satarug ◽  
Eimorn Mairiang ◽  
...  
Keyword(s):  
Bile Duct ◽  
Bile Duct Cancer ◽  
Liver Fluke ◽  
Northeast Thailand ◽  
Fluke Infection ◽  
Duct Cancer ◽  
Liver Fluke Infection

scholarly journals The Battle of Worldviews: A Case Study of Liver Fluke Infection in Khon Kaen, Thailand

2017 ◽  
Vol 22 (4) ◽  
pp. 902-908 ◽  
Author(s):  
Sara Samiphak ◽  
S. Leonard Syme
Keyword(s):  
Liver Fluke ◽  
Opisthorchis Viverrini ◽  
Case Study Method ◽  
Life And Death ◽  
Antiparasitic Drug ◽  
Parasitic Flatworm ◽  
Fundamental Cause ◽  
Fluke Infection ◽  
Liver Fluke Infection

Control efforts to reduce infection from the parasitic flatworm Opisthorchis viverrini have progressed through understanding the epidemiology of Opisthorchis viverrini, antiparasitic drug developments, technological innovations, health education promoting cooking of fish, and improved hygienic defecation. Yet the problem persists. The case study method was used to examine the fundamental cause of the liver fluke infection problem. Evidence shows that the liver fluke–infected population does not care about living a long life. For them, suffering and death are simply a part of life, and expected. Thus, the cause(s) leading to death is not important. They believe morally bad actions, and predetermined fate associated with kamma in Buddhism, play a big role whether or not one is infected with the liver fluke. Health interventions may be made more effective if they take into account the liver fluke–infected population’s worldviews about ethics, morality, life, and death. We researchers should not feel concerned only about medically determined causes of death.

scholarly journals Worm-free Cooking, Fish Safety: Recommendation to Strategy for Opisthorchiasis Prevention Program

2020 ◽  
pp. 65-73
Author(s):  
Rungrueng Kitphati ◽  
Thitima Wongsaroj ◽  
Choosak Nithikathkul
Keyword(s):  
Public Health ◽  
Freshwater Fish ◽  
Prevention And Control ◽  
Liver Fluke ◽  
Opisthorchis Viverrini ◽  
Basic Knowledge ◽  
Fluke Infection ◽  
Liver Fluke Infection ◽  
And Control ◽  
Viverrini Infection

Fish-borne parasitic zoonosis such as Opisthorchiasis caused by Opisthorchis viverrini remains a major public health problem in many parts of Southeast Asia and Me Kong Basin region including Thailand. The focal point of Opisthorchiasis is located in north-eastern part of Thailand, along with high prevalence coincidence of cholangiocarcinoma, a major primary carcinoma of the liver with a very poor prognosis. Opisthorchis viverrini infection caused by developed cholangiocarcinoma (CCA). O. viverrini infection is acquired by eating raw or partially cooked fish. In endemic areas, several food preparations contain uncooked or raw freshwater fish. The most common local freshwater fish used for this recipe are Koi Pla, Pla Som, Lab Pla and Pla Yang or Grill fish. Raw fish dishes are known to be associated with the risk of liver fluke infection due to the consumption of cyprinoid fish that contains metacercaria of O. viverrini. Grill fish and Lab-Pla are among the famous Thai traditional food in the northeastern and northern part of Thailand. The consumption of raw Grill fish can lead to    O. viverrini infection because cyprinoid fish is a second intermediate host of O. viverrini.  This study investigated the literature of previous working for prevention and control of Opisthorchiasis and confirm worm-free cooking safety of cooking with consideration of time for the preparation of freshwater fish-grill for the prevention and control of Opisthorchiasis and Cholangiocarcinoma infection. The unique experiment experimental designed. The freshwater fishes grill within the group of 0,1,2,3,4 and 5 minutes with 10 fresh water fishes from fisherman in Nakhon Phanom and Sakhon Nakorn provinces which reported high O. viverrini infection among risk people in 2009. The another experiment using frozen freshwater fish at -10 degree for 5 days. The results showed that metacercariae remained active in control and 1-2 minutes experimental groups. The groups 3-5 minutes of grill fish partially cooked showed inactive metacercariae. The excretory bladder spread with unclear form. The conclusion suggested that worm-free cooking under review, and unique experiment of cook safety is a crucial basic knowledge leading to bringing knowledge, practically for the prevention and control Liver fluke infection. Recommendation information also let people better understand the concerns during health literacy program to stop transmission of liver fluke and need to be discussed among head villagers, public health agencies and teachers in the public participation process and school health program. 

A tumorigenic cell line derived from a hamster cholangiocarcinoma associated with Opisthorchis felineus liver fluke infection

Life Sciences ◽  
2021 ◽  
pp. 119494
Author(s):  
Viatcheslav A. Mordvinov ◽  
Galina A. Minkova ◽  
Anna V. Kovner ◽  
Dmitriy V. Ponomarev ◽  
Maria N. Lvova ◽  
...  
Keyword(s):  
Cell Line ◽  
Liver Fluke ◽  
Opisthorchis Felineus ◽  
Fluke Infection ◽  
Liver Fluke Infection ◽  
Tumorigenic Cell Line ◽  
Tumorigenic Cell

scholarly journals Masculinity and misinformation: Social dynamics of liver fluke infection risk in Thailand

2021 ◽  
pp. 102382
Author(s):  
Yi-Chen Wang ◽  
Carl Grundy-Warr ◽  
Jutamas Namsanor ◽  
Miles Kenney-Lazar ◽  
Charlotte Jie Yi Tang ◽  
...  
Keyword(s):  
Liver Fluke ◽  
Social Dynamics ◽  
Infection Risk ◽  
Fluke Infection ◽  
Liver Fluke Infection

scholarly journals Predictive risk factors towards liver fluke infection among the people in Kamalasai District, Kalasin Province, Thailand

2019 ◽  
Vol 7 ◽  
pp. 205031211984020 ◽  
Author(s):  
Woragon Wichaiyo ◽  
Wirat Parnsila ◽  
Wisit Chaveepojnkamjorn ◽  
Banchob Sripa
Keyword(s):  
Risk Factors ◽  
Liver Fluke ◽  
Public Health Problem ◽  
Major Public Health Problem ◽  
Cross Sectional ◽  
The People ◽  
Fluke Infection ◽  
Raw Fish ◽  
Predictive Risk ◽  
Liver Fluke Infection

Background: Liver fluke disease caused by Opisthorchis viverrini remains a major public health problem with its crucial risk factors caused by some individual habits or false beliefs among the people in northeastern Thailand concerning the consumption of raw fish meat dishes. Objectives: This study explores the predictive risk factors for the infection of liver fluke disease. Methods: The sample consisted of 400 people aged 30 years and above in Thanya sub-district, Kamalasai district, Kalasin province. A cross-sectional analytic study, using the χ2 test, odds ratio and 95% confidence interval, was used to find the influence of each variable, along with the use of multiple logistic regression (p = 0.05). A questionnaire form was used as the research instrument. Results: Factors found in the results are as follows: households with a cat were 7.00 times more at risk than households without a cat; eating raw fish dishes prepared by themselves increases the risk of infection by 2.58 times; eating raw fish dishes prepared by family members increases the risk by 4.74 times; and raw fish dishes bought from a community market increases the risk by 2.33 times. Conclusion: A campaign should be launched to educate people not to eat raw or undercooked fish dishes, but to fully cook fish dishes before eating, as the food is still delicious, but also safe, healthy, and free from liver fluke infection.

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