Faculty Opinions recommendation of Dectin-1 activates Syk tyrosine kinase in a dynamic subset of macrophages for reactive oxygen production.

2005 ◽  
Author(s):  
Mary Dinauer
Keyword(s):  
Tyrosine Kinase ◽  
Reactive Oxygen ◽  
Oxygen Production

scholarly journals Dectin-1 activates Syk tyrosine kinase in a dynamic subset of macrophages for reactive oxygen production

Blood ◽  
2005 ◽  
Vol 106 (7) ◽  
pp. 2543-2550 ◽  
Author(s):  
David M. Underhill ◽  
Eddie Rossnagle ◽  
Clifford A. Lowell ◽  
Randi M. Simmons
Keyword(s):  
Immune Responses ◽  
Tyrosine Kinase ◽  
Intracellular Signaling ◽  
Protein Tyrosine Kinase ◽  
Reactive Oxygen ◽  
Oxygen Production ◽  
Cell Receptors ◽  
Lectin Receptor ◽  
Adaptive Immune ◽  
Inflammatory Activation

AbstractDectin-1 is a lectin receptor for β-glucan that is important for innate macrophage recognition of fungi and contributes to phagocytosis, reactive oxygen production, and induction of inflammatory cytokines. The mechanisms by which Dectin-1 mediates intracellular signaling are just beginning to be defined. Spleen tyrosine kinase (Syk) is a protein tyrosine kinase that is critical for adaptive immune responses where it mediates signaling through B-cell receptors, T-cell receptors, and Fc receptors. Here we report that Dectin-1 activates Syk in macrophages and is important for Dectin-1-stimulated reactive oxygen production, but not for phagocytosis. Syk activation is restricted to a subpopulation of macrophages that is in equilibrium with cells that cannot activate the pathway. The proportion of macrophages using this signaling pathway can be modulated by cytokine treatment. Thus, Dectin-1 signaling reveals dynamic macrophage heterogeneity in inflammatory activation potential. (Blood. 2005;106:2543-2550)

scholarly journals Tyrosine Kinase Activation in Response to Fungal Spores Is Primarily Dependent on Endogenous Reactive Oxygen Production in Macrophages

2000 ◽  
Vol 275 (14) ◽  
pp. 10175-10181 ◽  
Author(s):  
Tracy A. Shahan ◽  
William G. Sorenson ◽  
Janet Simpson ◽  
Nicholas A. Kefalides ◽  
Daniel M. Lewis
Keyword(s):  
Tyrosine Kinase ◽  
Fungal Spores ◽  
Reactive Oxygen ◽  
Oxygen Production ◽  
Kinase Activation

scholarly journals Activation of the Cytoplasmic c-Abl Tyrosine Kinase by Reactive Oxygen Species

2000 ◽  
Vol 275 (23) ◽  
pp. 17237-17240 ◽  
Author(s):  
Xiangao Sun ◽  
Pradip Majumder ◽  
Hisashi Shioya ◽  
Frank Wu ◽  
Shailendra Kumar ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Tyrosine Kinase ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Abl Tyrosine Kinase

scholarly journals Activation of Swelling-activated Chloride Current by Tumor Necrosis Factor-α Requires ClC-3-dependent Endosomal Reactive Oxygen Production

2010 ◽  
Vol 285 (30) ◽  
pp. 22864-22873 ◽  
Author(s):  
James J. Matsuda ◽  
Mohammed S. Filali ◽  
Jessica G. Moreland ◽  
Francis J. Miller ◽  
Fred S. Lamb
Keyword(s):  
Tumor Necrosis Factor ◽  
Tumor Necrosis ◽  
Tumor Necrosis Factor Α ◽  
Reactive Oxygen ◽  
Chloride Current ◽  
Oxygen Production ◽  
Factor Α ◽  
Necrosis Factor

scholarly journals PAC1 regulates receptor tyrosine kinase transactivation in a reactive oxygen species-dependent manner

Peptides ◽  
2019 ◽  
Vol 120 ◽  
pp. 170017
Author(s):  
Terry W. Moody ◽  
Lingaku Lee ◽  
Tatiana Iordanskaia ◽  
Irene Ramos-Alvarez ◽  
Paola Moreno ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Tyrosine Kinase ◽  
Receptor Tyrosine Kinase ◽  
Reactive Oxygen ◽  
Dependent Manner ◽  
Oxygen Species

scholarly journals Effect of single oral dose of azithromycin, clarithromycin, and roxithromycin on polymorphonuclear leukocyte function assessed ex vivo by flow cytometry.

1996 ◽  
Vol 40 (9) ◽  
pp. 2039-2042 ◽  
Author(s):  
C Wenisch ◽  
B Parschalk ◽  
K Zedtwitz-Liebenstein ◽  
A Weihs ◽  
I el Menyawi ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Oral Dose ◽  
Single Oral Dose ◽  
Ex Vivo ◽  
Reactive Oxygen ◽  
Fluorescein Isothiocyanate ◽  
Neutrophil Granulocytes ◽  
Oxygen Production ◽  
Phagocytic Cells ◽  
Phagocytic Capacity

Azithromycin was given as a single oral dose (20 mg/kg of body weight) to 12 volunteers in a crossover study with roxithromycin (8 to 12 mg/kg) and clarithromycin (8 to 12 mg/kg). Flow cytometry was used to study the phagocytic functions and the release of reactive oxygen products following phagocytosis by neutrophil granulocytes prior to administration of the three drugs, 16 h after azithromycin administration, and 3 h after clarithromycin and roxithromycin administration. Phagocytic capacity was assessed by measuring the uptake of fluorescein isothiocyanate-labeled bacteria. Reactive oxygen generation after phagocytosis of unlabeled bacteria was estimated by the amount of dihydrorhodamine 123 converted to rhodamine 123 intracellularly. Azithromycin resulted in decreased capacities of the cells to phagocytize Escherichia coli (median [range], 62% [27 to 91%] of the control values; P < 0.01) and generate reactive oxygen products (75% [34 to 26%] of the control values; P < 0.01). Clarithromycin resulted in reduced phagocytosis (82% [75 to 98%] of control values; P < 0.01) but did not alter reactive oxygen production (84% [63 to 113%] of the control values; P > 0.05). Roxithromycin treatment did not affect granulocyte phagocytosis (92% [62 to 118%] of the control values; P > 0.05) or reactive oxygen production (94% [66 to 128%] of the control value; P > 0.05). No relation between intra- and/or extracellular concentrations of azithromycin and/or roxithromycin and the polymorphonuclear phagocyte function and/or reactive oxygen production existed (P > 0.05 for all comparisons). These results demonstrate that the accumulation of macrolides in neutrophils can suppress the response of phagocytic cells to bacterial pathogens after a therapeutic dose.

scholarly journals The Involvement of the Tyrosine Kinase c-Src in the Regulation of Reactive Oxygen Species Generation Mediated by NADPH Oxidase-1

2008 ◽  
Vol 19 (7) ◽  
pp. 2984-2994 ◽  
Author(s):  
Davide Gianni ◽  
Ben Bohl ◽  
Sara A. Courtneidge ◽  
Gary M. Bokoch
Keyword(s):  
Reactive Oxygen Species ◽  
Nadph Oxidase ◽  
Tyrosine Kinase ◽  
Reactive Oxygen Species Generation ◽  
Reactive Oxygen ◽  
Tumor Formation ◽  
Ros Generation ◽  
Oxygen Species ◽  
Human Colon Carcinoma Cell ◽  
Kinase C

NADPH oxidase (Nox) family enzymes are one of the main sources of cellular reactive oxygen species (ROS), which have been shown to function as second messenger molecules. To date, seven members of this family have been reported, including Nox1-5 and Duox1 and -2. With the exception of Nox2, the regulation of the Nox enzymes is still poorly understood. Nox1 is highly expressed in the colon, and it requires two cytosolic regulators, NoxO1 and NoxA1, as well as the binding of Rac1 GTPase, for its activity. In this study, we investigate the role of the tyrosine kinase c-Src in the regulation of ROS formation by Nox1. We show that c-Src induces Nox1-mediated ROS generation in the HT29 human colon carcinoma cell line through a Rac-dependent mechanism. Treatment of HT29 cells with the Src inhibitor PP2, expression of a kinase-inactive form of c-Src, and c-Src depletion by small interfering RNA (siRNA) reduce both ROS generation and the levels of active Rac1. This is associated with decreased Src-mediated phosphorylation and activation of the Rac1-guanine nucleotide exchange factor Vav2. Consistent with this, Vav2 siRNA that specifically reduces endogenous Vav2 protein is able to dramatically decrease Nox1-dependent ROS generation and abolish c-Src-induced Nox1 activity. Together, these results establish c-Src as an important regulator of Nox1 activity, and they may provide insight into the mechanisms of tumor formation in colon cancers.

Sign in / Sign up

Export Citation Format

Share Document