Faculty Opinions recommendation of Fibroblast-led collective invasion of carcinoma cells with differing roles for RhoGTPases in leading and following cells.

2008 ◽  
Author(s):  
Kathleen J Green
Keyword(s):  
Carcinoma Cells ◽  
Collective Invasion

scholarly journals CD147 promotes collective invasion through cathepsin B in hepatocellular carcinoma

2020 ◽  
Author(s):  
Shi-Jie Wang ◽  
Dong Chao ◽  
Wei Wei ◽  
Gang Nan ◽  
Jia-Yue Li ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cathepsin B ◽  
Molecular Mechanisms ◽  
Three Dimensional ◽  
Carcinoma Cells ◽  
Human Hepatocellular Carcinoma ◽  
Migration And Invasion ◽  
Hepatocellular Carcinoma Cells ◽  
Collective Invasion ◽  
Invasion Model

Abstract Background: Mounting evidence suggests that solid tumors display the features of collective invasion, however, the molecular mechanisms are far from clear. This study aims to verify the role and the underlying mechanisms of CD147 in collective invasion in hepatocellular carcinoma.Methods: Immunostaining was used to analyze human hepatocellular carcinoma specimens and three-dimensional cultures. Three-dimensional invasion model was established to mimic in vivo invasion. RNA-sequencing was used to identify downstream effectors.Results: Human hepatocellular carcinoma undergone collective invasion and CD147 was observed to be upregulated at the invasive front of tumor cell groups. CD147 was demonstrated to promote collective invasion using the modified three-dimensional invasion model, which recapitulated the main features of collective invasion. Through transcriptome analysis and enzyme activity assay, we found that CD147 enhanced cathepsin B expression and activation. Upregulated cathepsin B in hepatocellular carcinoma cells facilitated migration and invasion, which mediated CD147-induced invasive phenotype in hepatocellular carcinoma. In terms of mechanism, we found that CD147 promoted cathepsin B transcription by activating β-catenin signaling as a result of reduced GSK-3β expression. Furthermore, we found that elevated expression of CD147 as well as cathepsin B were correlated with poor prognosis in patients with hepatocellular carcinoma.Conclusions: CD147 promotes hepatocellular carcinoma cells collective invasion via upregulating cathepsin B expression and targeting CD147 would be valuable for the development of novel therapeutic modalities against invasion and metastasis of cancer.

scholarly journals CD147 promotes collective invasion through cathepsin B in hepatocellular carcinoma

2020 ◽  
Author(s):  
Shi-Jie Wang ◽  
Dong Chao ◽  
Wei Wei ◽  
Gang Nan ◽  
Jia-Yue Li ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cathepsin B ◽  
Molecular Mechanisms ◽  
Three Dimensional ◽  
Carcinoma Cells ◽  
Human Hepatocellular Carcinoma ◽  
Migration And Invasion ◽  
Hepatocellular Carcinoma Cells ◽  
Collective Invasion ◽  
Invasion Model

Abstract Background Mounting evidence suggests that solid tumors display the features of collective invasion, however, the molecular mechanisms are far from clear. This study aims to verify the role and the underlying mechanisms of CD147 in collective invasion in hepatocellular carcinoma. Methods Immunostaining was used to analyze human hepatocellular carcinoma specimens and three-dimensional cultures. Three-dimensional invasion model was established to mimic in vivo invasion. RNA-sequencing was used to identify downstream effectors. Results Human hepatocellular carcinoma undergone collective invasion and CD147 was observed to be upregulated at the invasive front of tumor cell groups. CD147 was demonstrated to promote collective invasion using the modified three-dimensional invasion model, which recapitulated the main features of collective invasion. Through transcriptome analysis and enzyme activity assay, we found that CD147 enhanced cathepsin B expression and activation. Upregulated cathepsin B in hepatocellular carcinoma cells facilitated migration and invasion, which mediated CD147-induced invasive phenotype in hepatocellular carcinoma. In terms of mechanism, we found that CD147 promoted cathepsin B transcription by activating β-catenin signaling as a result of reduced GSK-3β expression. Furthermore, we found that elevated expression of CD147 as well as cathepsin B were correlated with poor prognosis in patients with hepatocellular carcinoma. Conclusions CD147 promotes hepatocellular carcinoma cells collective invasion via upregulating cathepsin B expression and targeting CD147 would be valuable for the development of novel therapeutic modalities against invasion and metastasis of cancer.

scholarly journals p120-catenin is required for the collective invasion of squamous cell carcinoma cells via a phosphorylation-independent mechanism

Oncogene ◽  
2007 ◽  
Vol 26 (36) ◽  
pp. 5214-5228 ◽  
Author(s):  
I R Macpherson ◽  
S Hooper ◽  
A Serrels ◽  
L McGarry ◽  
B W Ozanne ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Carcinoma Cells ◽  
P120 Catenin ◽  
Collective Invasion ◽  
Independent Mechanism

Fibroblast-led collective invasion of carcinoma cells with differing roles for RhoGTPases in leading and following cells

2007 ◽  
Vol 9 (12) ◽  
pp. 1392-1400 ◽  
Author(s):  
Cedric Gaggioli ◽  
Steven Hooper ◽  
Cristina Hidalgo-Carcedo ◽  
Robert Grosse ◽  
John F. Marshall ◽  
...  
Keyword(s):  
Carcinoma Cells ◽  
Collective Invasion

scholarly journals Collective invasion of carcinoma cells

2008 ◽  
Vol 2 (1) ◽  
pp. 45-47 ◽  
Author(s):  
Cedric Gaggioli
Keyword(s):  
Carcinoma Cells ◽  
Collective Invasion
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