Faculty Opinions recommendation of Complete viral genome sequence and discovery of novel viruses by deep sequencing of small RNAs: a generic method for diagnosis, discovery and sequencing of viruses.

Author(s):  
David Wang
Virology ◽  
2009 ◽  
Vol 388 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Jan F. Kreuze ◽  
Ana Perez ◽  
Milton Untiveros ◽  
Dora Quispe ◽  
Segundo Fuentes ◽  
...  

2021 ◽  
Author(s):  
Xiaofeng Dong ◽  
Hannah Goldswain ◽  
Rebekah Penrice-Randal ◽  
Ghada Shawli ◽  
Tessa Prince ◽  
...  

The mutational landscape of SARS-CoV-2 varies at both the dominant viral genome sequence and minor genomic variant population. An early change associated with transmissibility was the D614G substitution in the spike protein. This appeared to be accompanied by a P323L substitution in the viral polymerase (NSP12), but this latter change was not under strong selective pressure. Investigation of P323L/D614G changes in the human population showed rapid emergence during the containment phase and early surge phase of wave 1 in the UK. This rapid substitution was from minor genomic variants to become part of the dominant viral genome sequence. A rapid emergence of 323L but not 614G was observed in a non-human primate model of COVID-19 using a starting virus with P323 and D614 in the dominant genome sequence and 323L and 614G in the minor variant population. In cell culture, a recombinant virus with 323L in NSP12 had a larger plaque size than the same recombinant virus with P323. These data suggest that it may be possible to predict the emergence of a new variant based on tracking the distribution and frequency of minor variant genomes at a population level, rather than just focusing on providing information on the dominant viral genome sequence e.g., consensus level reporting. The ability to predict an emerging variant of SARS-CoV-2 in the global landscape may aid in the evaluation of medical countermeasures and non-pharmaceutical interventions.


Nature ◽  
2014 ◽  
Vol 513 (7517) ◽  
pp. 242-245 ◽  
Author(s):  
Li Deng ◽  
J. Cesar Ignacio-Espinoza ◽  
Ann C. Gregory ◽  
Bonnie T. Poulos ◽  
Joshua S. Weitz ◽  
...  

2021 ◽  
Vol 10 (8) ◽  
Author(s):  
Kirsty T. T. Kwok ◽  
Myrna M. T. de Rooij ◽  
Felisita F. Sinartio ◽  
Lidwien A. M. Smit ◽  
Marion P. G. Koopmans ◽  
...  

ABSTRACT We report the genome sequence of a Minacovirus strain identified from a fecal sample from a farmed mink (Neovison vison) in The Netherlands that was tested negative for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) using real-time PCR (RT-PCR). The viral genome sequence was obtained using agnostic deep sequencing.


2015 ◽  
pp. 809-816
Author(s):  
Mari C. Vives ◽  
Karelia Velázquez ◽  
José A. Pina ◽  
Pedro Moreno ◽  
José Guerri ◽  
...  

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Murray Sharman ◽  
Monica Kehoe ◽  
Brenda Coutts ◽  
Joop van Leur ◽  
Fiona Filardo ◽  
...  

We present here the complete genome sequences of a novel polerovirus from Trifolium subterraneum (subterranean clover) and Cicer arietinum (chickpea) and compare these to a partial viral genome sequence obtained from Macroptilium lathyroides (phasey bean). We propose the name phasey bean mild yellows virus for this novel polerovirus.


Gene ◽  
2021 ◽  
Vol 779 ◽  
pp. 145496
Author(s):  
Kunikazu Hoshino ◽  
Tatsuji Maeshiro ◽  
Nao Nishida ◽  
Masaya Sugiyama ◽  
Jiro Fujita ◽  
...  

2020 ◽  
Vol 9 (5) ◽  
Author(s):  
Byung-Joo Park ◽  
Hee-Seop Ahn ◽  
Sang-Hoon Han ◽  
Hyeon-Jeong Go ◽  
Eu-Lim Lyoo ◽  
...  

A human norovirus (HuNoV) strain was obtained from a patient with acute gastroenteritis, and its complete coding sequence was determined. The coding-complete viral genome, with three open reading frames, was 7,565 bp long, with a GC content of 49.9%. The genotype of the HuNoV strain obtained in this study was identified as GII.p12_GII.3.


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