Faculty Opinions recommendation of Quantitative live cell imaging reveals a gradual shift between DNA repair mechanisms and a maximal use of HR in mid S phase.

2014 ◽  
Author(s):  
Wade Clapp ◽  
Grzegorz Nalepa
Keyword(s):  
Dna Repair ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
S Phase ◽  
Live Cell ◽  
Dna Repair Mechanisms ◽  
Gradual Shift ◽  
Repair Mechanisms

scholarly journals Quantitative Live Cell Imaging Reveals a Gradual Shift between DNA Repair Mechanisms and a Maximal Use of HR in Mid S Phase

2012 ◽  
Vol 47 (2) ◽  
pp. 320-329 ◽  
Author(s):  
Ketki Karanam ◽  
Ran Kafri ◽  
Alexander Loewer ◽  
Galit Lahav
Keyword(s):  
Dna Repair ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
S Phase ◽  
Live Cell ◽  
Dna Repair Mechanisms ◽  
Gradual Shift ◽  
Repair Mechanisms

Single-molecule live-cell imaging of bacterial DNA repair and damage tolerance

2017 ◽  
Vol 46 (1) ◽  
pp. 23-35 ◽  
Author(s):  
Harshad Ghodke ◽  
Han Ho ◽  
Antoine M. van Oijen
Keyword(s):  
Dna Repair ◽  
Single Molecule ◽  
Live Cell Imaging ◽  
Spatial Organization ◽  
Cell Imaging ◽  
Live Cell ◽  
Live Cells ◽  
Temporal Regulation ◽  
Repair Processes ◽  
Wide Range

Genomic DNA is constantly under threat from intracellular and environmental factors that damage its chemical structure. Uncorrected DNA damage may impede cellular propagation or even result in cell death, making it critical to restore genomic integrity. Decades of research have revealed a wide range of mechanisms through which repair factors recognize damage and co-ordinate repair processes. In recent years, single-molecule live-cell imaging methods have further enriched our understanding of how repair factors operate in the crowded intracellular environment. The ability to follow individual biochemical events, as they occur in live cells, makes single-molecule techniques tremendously powerful to uncover the spatial organization and temporal regulation of repair factors during DNA–repair reactions. In this review, we will cover practical aspects of single-molecule live-cell imaging and highlight recent advances accomplished by the application of these experimental approaches to the study of DNA–repair processes in prokaryotes.

scholarly journals Assembly of multiprotein complexes that control genome function

2009 ◽  
Vol 185 (1) ◽  
pp. 21-26 ◽  
Author(s):  
Christoffel Dinant ◽  
Martijn S. Luijsterburg ◽  
Thomas Höfer ◽  
Gesa von Bornstaedt ◽  
Wim Vermeulen ◽  
...  
Keyword(s):  
Mathematical Modeling ◽  
Dna Repair ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Imaging Studies ◽  
Multiprotein Complexes ◽  
Insight Into

Live-cell imaging studies aided by mathematical modeling have provided unprecedented insight into assembly mechanisms of multiprotein complexes that control genome function. Such studies have unveiled emerging properties of chromatin-associated systems involved in DNA repair and transcription.

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