Faculty Opinions recommendation of High-throughput metagenomic technologies for complex microbial community analysis: open and closed formats.

2015 ◽  
Author(s):  
Oded Beja ◽  
Alon Philosof
Keyword(s):  
Microbial Community ◽  
High Throughput ◽  
Community Analysis ◽  
Microbial Community Analysis ◽  
Complex Microbial Community

scholarly journals Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms

2012 ◽  
Vol 6 (8) ◽  
pp. 1621-1624 ◽  
Author(s):  
J Gregory Caporaso ◽  
Christian L Lauber ◽  
William A Walters ◽  
Donna Berg-Lyons ◽  
James Huntley ◽  
...  
Keyword(s):  
Microbial Community ◽  
High Throughput ◽  
Community Analysis ◽  
Microbial Community Analysis ◽  
Illumina Hiseq

scholarly journals High-Throughput Metagenomic Technologies for Complex Microbial Community Analysis: Open and Closed Formats

mBio ◽  
2015 ◽  
Vol 6 (1) ◽  
Author(s):  
Jizhong Zhou ◽  
Zhili He ◽  
Yunfeng Yang ◽  
Ye Deng ◽  
Susannah G. Tringe ◽  
...  
Keyword(s):  
Microbial Communities ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Community Analysis ◽  
Microbial Community Analysis ◽  
Natural Environments ◽  
Advantages And Disadvantages ◽  
Complex Microbial Community ◽  
Microbial Systems ◽  
Century Science

ABSTRACT  Understanding the structure, functions, activities and dynamics of microbial communities in natural environments is one of the grand challenges of 21st century science. To address this challenge, over the past decade, numerous technologies have been developed for interrogating microbial communities, of which some are amenable to exploratory work (e.g., high-throughput sequencing and phenotypic screening) and others depend on reference genes or genomes (e.g., phylogenetic and functional gene arrays). Here, we provide a critical review and synthesis of the most commonly applied “open-format” and “closed-format” detection technologies. We discuss their characteristics, advantages, and disadvantages within the context of environmental applications and focus on analysis of complex microbial systems, such as those in soils, in which diversity is high and reference genomes are few. In addition, we discuss crucial issues and considerations associated with applying complementary high-throughput molecular technologies to address important ecological questions.

Microbial Community Analysis Using High-Throughput Amplicon Sequencing

2015 ◽  
pp. 2.4.2-1-2.4.2-26 ◽  
Author(s):  
Danny Ionescu ◽  
Will A. Overholt ◽  
Michael D. J. Lynch ◽  
Josh D. Neufeld ◽  
Ankur Naqib ◽  
...  
Keyword(s):  
Microbial Community ◽  
High Throughput ◽  
Community Analysis ◽  
Amplicon Sequencing ◽  
Microbial Community Analysis

Reducing bias in complex microbial community analysis in shrimp based on propidium monoazide combined with PCR-DGGE

Food Control ◽  
2016 ◽  
Vol 68 ◽  
pp. 139-144 ◽  
Author(s):  
Fei Zhao ◽  
Haiquan Liu ◽  
Zhaohuan Zhang ◽  
Lili Xiao ◽  
Xiaohong Sun ◽  
...  
Keyword(s):  
Microbial Community ◽  
Community Analysis ◽  
Microbial Community Analysis ◽  
Propidium Monoazide ◽  
Complex Microbial Community
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