Faculty Opinions recommendation of Amnioserosa cell constriction but not epidermal actin cable tension autonomously drives dorsal closure.

2016 ◽  
Author(s):  
Ronen Zaidel-Bar
Keyword(s):  
Dorsal Closure ◽  
Cable Tension ◽  
Actin Cable

Amnioserosa cell constriction but not epidermal actin cable tension autonomously drives dorsal closure

2016 ◽  
Vol 18 (11) ◽  
pp. 1161-1172 ◽  
Author(s):  
Laurynas Pasakarnis ◽  
Erich Frei ◽  
Emmanuel Caussinus ◽  
Markus Affolter ◽  
Damian Brunner
Keyword(s):  
Dorsal Closure ◽  
Cable Tension ◽  
Actin Cable

scholarly journals Dynamic Analysis of Actin Cable Function during Drosophila Dorsal Closure

2002 ◽  
Vol 12 (14) ◽  
pp. 1245-1250 ◽  
Author(s):  
Antonio Jacinto ◽  
William Wood ◽  
Sarah Woolner ◽  
Charlotte Hiley ◽  
Laura Turner ◽  
...  
Keyword(s):  
Dynamic Analysis ◽  
Dorsal Closure ◽  
Actin Cable

scholarly journals The single Drosophila ZO-1 protein Polychaetoid regulates embryonic morphogenesis in coordination with Canoe/afadin and Enabled

2011 ◽  
Vol 22 (12) ◽  
pp. 2010-2030 ◽  
Author(s):  
Wangsun Choi ◽  
Kuo-Chen Jung ◽  
Kevin S. Nelson ◽  
Manzoor A. Bhat ◽  
Greg J. Beitel ◽  
...  
Keyword(s):  
Protein Localization ◽  
Leading Edge ◽  
Null Alleles ◽  
Dorsal Closure ◽  
Genetic Redundancy ◽  
Tracheal System ◽  
Genetic Studies ◽  
Cell Behaviors ◽  
Actin Cable ◽  
Embryonic Morphogenesis

Adherens and tight junctions play key roles in assembling epithelia and maintaining barriers. In cell culture zonula occludens (ZO)–family proteins are important for assembly/maturation of both tight and adherens junctions (AJs). Genetic studies suggest that ZO proteins are important during normal development, but interpretation of mouse and fly studies is limited by genetic redundancy and/or a lack of null alleles. We generated null alleles of the single Drosophila ZO protein Polychaetoid (Pyd). Most embryos lacking Pyd die with striking defects in morphogenesis of embryonic epithelia including the epidermis, segmental grooves, and tracheal system. Pyd loss does not dramatically affect AJ protein localization or initial localization of actin and myosin during dorsal closure. However, Pyd loss does affect several cell behaviors that drive dorsal closure. The defects, which include segmental grooves that fail to retract, a disrupted leading edge actin cable, and reduced zippering as leading edges meet, closely resemble defects in canoe zygotic null mutants and in embryos lacking the actin regulator Enabled (Ena), suggesting that these proteins act together. Canoe (Cno) and Pyd are required for proper Ena localization during dorsal closure, and strong genetic interactions suggest that Cno, Pyd, and Ena act together in regulating or anchoring the actin cytoskeleton during dorsal closure.

Cable Tension Control of Cable-Stayed Bridge Using Fuzzy Satisfaction Method and Genetic Algorithm

2001 ◽  
Vol 84 (3) ◽  
pp. 39-46
Author(s):  
Hitoshi Furuta ◽  
Masakatsu Kaneyoshi ◽  
Hiroshi Tanaka ◽  
Eiichi Watanabe
Keyword(s):  
Genetic Algorithm ◽  
Tension Control ◽  
Cable Tension ◽  
Cable Stayed Bridge

scholarly journals The AF-6 Homolog Canoe Acts as a Rap1 Effector During Dorsal Closure of the Drosophila Embryo

Genetics ◽  
2003 ◽  
Vol 165 (1) ◽  
pp. 159-169
Author(s):  
Benjamin Boettner ◽  
Phoebe Harjes ◽  
Satoshi Ishimaru ◽  
Michael Heke ◽  
Hong Qing Fan ◽  
...  
Keyword(s):  
Molecular Mechanisms ◽  
Genetic Interaction ◽  
Critical Role ◽  
Adherens Junction ◽  
Small Gtpases ◽  
Drosophila Embryo ◽  
Dorsal Closure ◽  
Cell Sheets ◽  
Jnk Pathway

Abstract Rap1 belongs to the highly conserved Ras subfamily of small GTPases. In Drosophila, Rap1 plays a critical role in many different morphogenetic processes, but the molecular mechanisms executing its function are unknown. Here, we demonstrate that Canoe (Cno), the Drosophila homolog of mammalian junctional protein AF-6, acts as an effector of Rap1 in vivo. Cno binds to the activated form of Rap1 in a yeast two-hybrid assay, the two molecules colocalize to the adherens junction, and they display very similar phenotypes in embryonic dorsal closure (DC), a process that relies on the elongation and migration of epithelial cell sheets. Genetic interaction experiments show that Rap1 and Cno act in the same molecular pathway during DC and that the function of both molecules in DC depends on their ability to interact. We further show that Rap1 acts upstream of Cno, but that Rap1, unlike Cno, is not involved in the stimulation of JNK pathway activity, indicating that Cno has both a Rap1-dependent and a Rap1-independent function in the DC process.

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