Faculty Opinions recommendation of Single-cell RNA sequencing reveals a signature of sexual commitment in malaria parasites.

2017 ◽  
Author(s):  
Weiqing Pan ◽  
Qingfeng Zhang
Keyword(s):  
Single Cell ◽  
Rna Sequencing ◽  
Malaria Parasites ◽  
Single Cell Rna Sequencing

scholarly journals Single-cell RNA sequencing reveals a signature of sexual commitment in malaria parasites

Nature ◽  
2017 ◽  
Vol 551 (7678) ◽  
pp. 95-99 ◽  
Author(s):  
Asaf Poran ◽  
Christopher Nötzel ◽  
Omar Aly ◽  
Nuria Mencia-Trinchant ◽  
Chantal T. Harris ◽  
...  
Keyword(s):  
Single Cell ◽  
Rna Sequencing ◽  
Malaria Parasites ◽  
Single Cell Rna Sequencing

scholarly journals Single-cell RNA-seq reveals hidden transcriptional variation in malaria parasites

eLife ◽  
2018 ◽  
Vol 7 ◽  
Author(s):  
Adam J Reid ◽  
Arthur M Talman ◽  
Hayley M Bennett ◽  
Ana R Gomes ◽  
Mandy J Sanders ◽  
...  
Keyword(s):  
Life Cycle ◽  
Single Cell ◽  
Rna Sequencing ◽  
Immune Evasion ◽  
Gene Families ◽  
Sexual Life ◽  
Malaria Parasites ◽  
Life Cycle Stages ◽  
Transcriptional Variation ◽  
Single Cell Rna Sequencing

Single-cell RNA-sequencing is revolutionising our understanding of seemingly homogeneous cell populations but has not yet been widely applied to single-celled organisms. Transcriptional variation in unicellular malaria parasites from the Plasmodium genus is associated with critical phenotypes including red blood cell invasion and immune evasion, yet transcriptional variation at an individual parasite level has not been examined in depth. Here, we describe the adaptation of a single-cell RNA-sequencing (scRNA-seq) protocol to deconvolute transcriptional variation for more than 500 individual parasites of both rodent and human malaria comprising asexual and sexual life-cycle stages. We uncover previously hidden discrete transcriptional signatures during the pathogenic part of the life cycle, suggesting that expression over development is not as continuous as commonly thought. In transmission stages, we find novel, sex-specific roles for differential expression of contingency gene families that are usually associated with immune evasion and pathogenesis.

41-OR: Single Cell RNA-Sequencing Reveals the Developmental Heterogeneity of Brown Adipocytes

Diabetes ◽  
2020 ◽  
Vol 69 (Supplement 1) ◽  
pp. 41-OR
Author(s):  
FARNAZ SHAMSI ◽  
MARY PIPER ◽  
LI-LUN HO ◽  
TIAN LIAN HUANG ◽  
YU-HUA TSENG
Keyword(s):  
Single Cell ◽  
Rna Sequencing ◽  
Brown Adipocytes ◽  
Single Cell Rna Sequencing
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