scholarly journals Transdermal Delivery of Capsaicin Nanoemulgel: Optimization, Skin Permeation and in Vivo Activity Against Diabetic Neuropathy

2021 ◽  
Author(s):  
May Saab ◽  
Karim Raafat ◽  
Hoda El-Maradny
Keyword(s):  
Diabetes Mellitus ◽  
Type 1 Diabetes ◽  
Diabetic Neuropathy ◽  
Type 1 Diabetes Mellitus ◽  
Isopropyl Alcohol ◽  
Skin Permeation ◽  
Tween 80 ◽  
Diabetic Mice ◽  
Therapeutic Benefits

Purpose: Diabetic somatic neuropathy is one of the most prevalent complications in type 1 diabetes mellitus. Many treatments were investigated to alleviate the pain associated with this condition. Capsaicin is a naturally occurring lipophilic alkaloid that proved to be an effective and safe treatment of chronic painful disorders. Despite the known therapeutic benefits of capsaicin, the conventional topical formulations have limited bioavailability. Therefore, the current study aims to develop capsaicin nanoemulgel to increase skin permeation and enhance its activity against neuropathic pain. Methods: Low-energy emulsification method was used to prepare nanoemulsions, using eucalyptus oil as the oily phase, tween 80 as a surfactant, propylene glycol, ethanol and isopropyl alcohol as co-surfactants. Pseudo-ternary phase diagrams were constructed to investigate and optimize the formulation. Subsequently, the optimum formulation was formulated as a nanoemulgel and investigated for, skin permeation using Franz diffusion cell, and diabetic neuropathy management using alloxan-induced diabetic mice. Results: The selected nanoemulsion containing 0.05% capsaicin is composed of 8 % oil, 24 % Smix (Tween 80: isopropyl alcohol 2:1 w/w) and 68 % water. It is characterized by nanosized globules (28.15±0.24 nm) with a relatively low polydispersity index (0.27±0.05). The nanoemulgel revealed circa 4-fold increase in capsaicin cumulative permeation when compared to the conventional gel, and an improvement in its antinociceptive properties was observed in the treated diabetic mice (p<0.05). Conclusion: The selected capsaicin nanoemulgel would be a promising transdermal formulation that may alleviate diabetic neuropathy in type 1 diabetes mellitus patients.

Diabetic neuropathy is associated with activation of the TNF-alpha system in subjects with type 1 diabetes mellitus

2005 ◽  
Vol 63 (5) ◽  
pp. 525-529 ◽  
Author(s):  
J. M. Gonzalez-Clemente ◽  
D. Mauricio ◽  
C. Richart ◽  
M. Broch ◽  
A. Caixas ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 1 Diabetes ◽  
Diabetic Neuropathy ◽  
Type 1 Diabetes Mellitus ◽  
Tnf Alpha

scholarly journals ID: 1079 Improved glucose homeostasis effect of conditioned media from adipose-derived stem cells in type 1 diabetic mice

2017 ◽  
Vol 4 (S) ◽  
pp. 165
Author(s):  
Anh Nguyen Tu Bui ◽  
Cong Le Thanh Nguyen ◽  
Ngoc Kim Phan ◽  
Loan Thi-Tung Dang
Keyword(s):  
Diabetes Mellitus ◽  
Type 1 Diabetes ◽  
Stem Cell ◽  
Blood Glucose ◽  
Blood Glucose Level ◽  
Type 1 Diabetes Mellitus ◽  
Pancreatic Islets ◽  
Conditioned Medium ◽  
Diabetic Mice

Background: Many studies suggested adipose derived stem cell (ASC) transplantation as a new approach to control hyperglycemia in type 1 diabetes mellitus. It is proposed that the effects of these cells could be not only based on the direct cell-cell interaction but also the secretion of cytokines. This study aimed to demonstrate the effect of adipose stem cell-derived conditioned medium (CM) on the treatment of STZ-induced diabetic mice.  Methods: CM was obtained from 24-hours-cultured medium of ASCs and centrifuged to remove the debris. Type 1 diabetic mice were intraperitoneally injected CM for 30 consecutive days. Therapeutic efficacy of CM was assessed by survival rate, blood glucose level, serum insulin level, histological structure of pancreatic islets. Results: The results showed that CM treatment could decrease mortality rate (from 33,33% to 0%) as well as blood glucose level (from 425,667±65,753 mg/dl to 203,500 mg ±20,350 mg/dl) and enhance insulin secretion, improve size and function of pancreatic islets of diabetic mice.  Conclusion: Conditioned medium maybe a promising therapy for type 1 diabetes mellitus.

scholarly journals Regenerative and Transplantation Medicine: Cellular Therapy Using Adipose Tissue-Derived Mesenchymal Stromal Cells for Type 1 Diabetes Mellitus

2019 ◽  
Vol 8 (2) ◽  
pp. 249 ◽  
Author(s):  
Hiroyuki Takahashi ◽  
Naoaki Sakata ◽  
Gumpei Yoshimatsu ◽  
Suguru Hasegawa ◽  
Shohta Kodama
Keyword(s):  
Diabetes Mellitus ◽  
Type 1 Diabetes ◽  
Adipose Tissue ◽  
Insulin Therapy ◽  
Type 1 Diabetes Mellitus ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Cellular Therapy ◽  
Therapeutic Benefits

Type 1 diabetes mellitus (T1DM) is caused by the autoimmune targeting of pancreatic β-cells, and, in the advanced stage, severe hypoinsulinemia due to islet destruction. In patients with T1DM, continuous exogenous insulin therapy cannot be avoided. However, an insufficient dose of insulin easily induces extreme hyperglycemia or diabetic ketoacidosis, and intensive insulin therapy may cause hypoglycemic symptoms including hypoglycemic shock. While these insulin therapies are efficacious in most patients, some additional therapies are warranted to support the control of blood glucose levels and reduce the risk of hypoglycemia in patients who respond poorly despite receiving appropriate treatment. There has been a recent gain in the popularity of cellular therapies using mesenchymal stromal cells (MSCs) in various clinical fields, owing to their multipotentiality, capacity for self-renewal, and regenerative and immunomodulatory potential. In particular, adipose tissue-derived MSCs (ADMSCs) have become a focus in the clinical setting due to the abundance and easy isolation of these cells. In this review, we outline the possible therapeutic benefits of ADMSC for the treatment of T1DM.

Effects of a Ganoderma atrum polysaccharide against pancreatic damage in streptozotocin-induced diabetic mice

2019 ◽  
Vol 10 (11) ◽  
pp. 7227-7238 ◽  
Author(s):  
Lu Li ◽  
Rui-Cheng Li ◽  
Ye-Hao Song ◽  
Wen-Ying Wu ◽  
Shu-Hua Yin ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 1 Diabetes ◽  
Type 1 Diabetes Mellitus ◽  
Diabetic Mice ◽  
Pancreatic Damage

This study aimed at exploring the role of a Ganoderma atrum polysaccharide (PSG-1) in pancreatic damage in streptozotocin (STZ)-induced type 1 diabetes mellitus (T1DM) mice.

Sign in / Sign up

Export Citation Format

Share Document