scholarly journals The Antibacterial Activity Of Several Sponges From The Waters Of Tasik Ria Against Escherichia coli And Staphylococcus aureus bacteria

2018 ◽  
Vol 7 (1) ◽  
pp. 1
Author(s):  
Abrianto A. O. Rompis ◽  
Fitje Losung ◽  
Deiske A. Sumilat ◽  
Agung B. Windarto ◽  
Stenly Wullur ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Natural Compounds ◽  
E Coli ◽  
Escherichia Coli Bacteria ◽  
And Staphylococcus Aureus ◽  
Positive Results ◽  
Sponge Extract

The sponge is one of the sea organisms that has a prospect as a source of natural compounds including peptides, steroids, asetogenin, terpenoids, alkaloids, cyclic halide and nitrogen. This research was directed to obtain several species of sponges from the waters of Tasik Ria as well as testing the antibacterial activity of extracts from some of the sponge against the bacteria Escherichia coli and Staphylococcus aureus. From the identification, seven species of sponges were found, which consists of: Amphimedon sp., Axinosa sp., Aaptos sp., Theonella sp., Cribochalina sp., Hyrtios sp., and Lendenfeldia sp. The tests of antibacterial activity of the extracts from these sponges against test bacteria E. coli and S. aureus showed some positive results. Extract from Axinosa sp. sponge(16 mm) showed the strongest antibacterial activity on Escherichia coli bacteria. Followed by Hyrtios sp. extract (13.5 mm), Aaptos sp. extract (13 mm), Lendenfeldia sp. extract (13 mm) and Cribochalinai sp. extract(10.5 mm). While the the tests on Staphylococcus aureus bacteria showed that the strongest antibacterial activity was found from Axinosa sp. sponge extract (16.5 mm), followed by the extract from Aaptos sp. (15 mm), Lendenfeldia sp. extract (14.5 mm), Hyrtios sp. extract(13.5 mm) and Cribochalina sp. extract (11 mm).Keywords: Sponge, antibacterial, Escherichia coli, Staphylococcus aureus ABSTRAK Spons merupakan salah satu biota laut yang sangat prospektif sebagai sumber senyawa bahan-bahan alami antara lain peptide, terpenoid, steroid, asetogenin, alkaloid, halide siklik dan senyawa nitrogen. Penelitian ini diarahkan untuk mendapatkan beberapa spesies spons dari perairan Tasik Ria serta menguji aktivitas antibakteri dari beberapa ekstrak spons terhadap bakteri Escherichia coli dan bakteri Staphylococcus aureus. Hasil identifikasi spons ditemukan sebanyak tujuh spesies yang terdiri dari: Amphimedon sp., Axinosa sp., Aaptos sp., Theonella sp., Hyrtios sp., Cribochalina sp. dan Lendenfeldia sp.. Aktivitas antibakteri dari beberapa ekstrak spons terhadap bakteri uji E. coli dan S. aureus terdapat diameter zona hambat bervariasi yaitu bakteri Escherichia coli menunjukkan aktivitas antibakteri ekstrak spons terkuat pada spons Axinosa sp (16 mm), disusul ekstrak spons Hyrtios sp. (13,5 mm), ekstrak spons Aaptos sp. (13 mm), ekstrak spons Lendenfeldia sp. (13 mm) dan ekstrak spons Cribochalinai sp. (10,5 mm).  Sedangkan pada bakteri Staphylococcus aureus menunjukkan aktivitas antibakteri ekstrak spons terkuat yaitu:  ekstrak spons Axinosa sp. (16,5 mm), disusul ekstrak spons Aaptos sp. (15 mm), ekstrak spons Lendenfeldia sp. (14,5 mm), ekstrak spons Hyrtios sp. (13,5 mm) dan ekstrak spons Cribochalina sp.(11mm).Kata Kunci : Spons, Antibakteri, Escherichia coli, Staphylococcus aureus

scholarly journals UJI AKTIVITAS ANTIBAKTERI JAMUR ENDOFIT PADA DAUN DAN BATANG TUMBUHAN KUMIS KUCING (Orthosiphon aristatus) TERHADAP BAKTERI Escherichia coli DAN staphylococcus aureus

PHARMACON ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 211
Author(s):  
Excel Pangouw ◽  
Jimmy Posangi ◽  
Widya Astuty Lolo ◽  
Robert Bara
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Endophytic Fungi ◽  
Antibacterial Effect ◽  
E Coli ◽  
Average Value ◽  
Escherichia Coli Bacteria ◽  
Cat’S Whiskers ◽  
And Staphylococcus Aureus

ABSTRACTEndophytic fungi are fungi that live in plant tissues and do not harm the plants. Endophytic fungi can produce compounds that have the potential to be antibacterial. This study aims to examine the antibacterial activity of endophytic fungi isolated from the leaves and stems of the cat's whiskers plant (Orthosiphon aristatus) against Escherichia coli and Staphylococcus aureus bacteria. Cat's whiskers contain essential oils which are used as antibacterial against some pathogenic bacteria such as Staphylococcus aureus and Escherichia coli. The method used is experimentally in the laboratory to test the antibacterial activity of endophytic fungi isolates derived from cat's whiskers (Orthosiphon aristatus) on Escherichia coli and Staphylococcus aureus bacteria using the hole / well method. In the research results obtained four samples of endophytic fungi extract isolated from the leaves and stems of the cat's whiskers plant (Orthosiphon aristatus) . The results of antibacterial research showed that endophytic mushroom extract isolated from cat's whiskers were more effective in inhibiting Escherichia coli bacteria compared to Staphylococcus aureus. In Escherichia coli bacteria the average value of inhibition is 5 mm whereas in Staphylococcus aureus bacteria the average value of inhibition is only 2 mm. So it can be seen that the endophytic fungus from the leaves of cat's whiskers has a better antibacterial effect than the endophytic fungus from the stems of the cat's whiskets. Conclusion, etidophytic fungi isolated from leaves and stems of cat's whiskers (Orthosiphon aristatus) have an antibacterial effect on the growth of E. coli bacteria and are not effective against S. aureus bacteria.Keywords: Cat's whiskers (Orthasiphan aristatus), antibacterial activity, Escherichia coli and Staphylococcus aureus. ABSTRAKJamur endofit ialah jamur yang hidup di dalam jaringan tumbuhan dan tidak membahayakan tumbuhan tersebut. Jamur endofit dapat menghasilkan senyawa yang berpotensi sebagai antibakteri. Penelitian ini bertujuan untuk menguji aktivitas antibakteri jamur endofit yang diisolasi dari daun dan batang tumbuhan kumis kucing (Orthosiphon aristatus) terhadap Bakteri Escherichia coli dan Staphylococcus aureus. Kumis kucing mengandung minyak atsiri yang digunakan sebagai antibakteri terhadap beberapa bakteri patogen seperti Staphylococcus aureus dan Escherichia coli. Metode yang dipakai yakni secara eksperimen di laboratorium untuk menguji adanya aktivitas antibakteri dari isolat jamur endofit  yang diberasal dari tumbuhan kumis kucing (Orthosiphon aristatus)  pada bakteri  Escherichia coli dan Staphylococcus aureus dengan menggunakan metode lubang/sumuran. Pada hasil penelitan didapatkan empat sampel ekstrak jamur endofit yang diisolasi dari daun dan batang  tumbuhan kumis kucing (Orthosiphon aristatus). Hasil penelitian antibakteri menunjukkan bahwa ekstrak jamur endofit yang diisolasi dari tumbuhan kumis kucing lebih efektif menghambat  bakteri Escherichia coli dibandingkan dengan bakteri Staphylococcus aureus. Pada bakteri  Escherichia coli nilai rata-rata daya hambat yaitu 5 mm sedangkan pada bakteri Staphylococcus aureus nilai rata-rata daya hambat yaitu hanya 2 mm. Sehingga dapat diketahui bahwa jamur endofit dari daun kumis kucing memiliki efek antibakteri yang lebih baik dibandingkan dengan jamur endofit dari batang kumis kucing. Kesimpulan, Jamur endofit yang diisolasi dari daun  dan batang kumis kucing (Orthosiphon aristatus) memiliki efek antibakteri terhadap pertumbuhan bakteri E. coli dan tidak efektiv terhadap bakteri S. aureus.Kata Kunci:  Tumbuhan kumis kucing (Orthosiphon aristatus), aktivitas antibakteri, Escherichia coli dan Staphylococcu saureus.

scholarly journals Uji Daya Hambat Ekstrak Metanol Beberapa Jenis Porifera Terhadap Bakteri Escherichia coli dan Staphylococcus aureus

Jurnal MIPA ◽  
2014 ◽  
Vol 3 (2) ◽  
pp. 129
Author(s):  
Megawati S. Saroinsong ◽  
Febby E. F. Kandou ◽  
Adelfia Papu ◽  
Marina F. O. Singkoh
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Methanol Extract ◽  
Inhibition Zone ◽  
E Coli ◽  
Activity Test ◽  
And Staphylococcus Aureus ◽  
Paper Disc ◽  
Coli Growth

Penelitian ini bertujuan untuk menguji daya hambat dari ekstrak metanol beberapa jenis porifera terhadap pertumbuhan bakteri E. coli dan S. aureus. Pengujian aktivitas antibakteri menggunakan metode Kirby-Bauer, yaitu dilakukan dengan mengukur zona hambat di sekeliling cakram kertas. Ekstrak Haliclona sp dapat menghambat pertumbuhan S. aureus pada konsentrasi ekstrak 30%, 60% dan 90% dengan hasil pengukuran diameter zona hambat 13.50 mm, 20.67 mm dan 27.33 mm; serta menghambat pertumbuhan E. coli dengan diameter zona hambat 10.08 mm, 12.83 mm dan 14.17 mm. Daya hambat ekstrak Agelas sp terhadap S. aureus menunjukkan diameter zona hambat 8.33 mm dan hanya menunjukkan pada konsentrasi ekstrak 90%, sementara daya hambat ekstrak Agelas sp pada konsentrasi 30%, 60% dan 90% terhadap E. coli menunjukkan diameter zona hambat sebesar 7.67 mm, 10.17 mm dan 14.17 mm. Daya hambat Spheciospongia sp terhadap S. aureus dan E. coli hanya terlihat pada konsentrasi ekstrak sebesar 90% dengan diameter zona hambat adalah 8.42 mm dan 8.75 mm. Berdasarkan hasil yang diperoleh, dapat disimpulkan bahwa ekstrak Haliclona sp memiliki potensi aktivitas antibakteri yang dapat digunakan sebagai bahan dasar pembuatan obat antibiotik.This research aimed to test the inhibition capabilities of methanol extract from several kinds of Porifera on Escherichia coli and Staphylococcus aureus growth. The antibacterial activity test using the Kirby-Bauer method, which delivered by measuring the inhibition zone around paper disc. The extract of Haliclona sp can inhibit the S. aureus growth at 30%, 60% and 90% of extracts concentration with the measurement of inhibition zone diameters are 13.50 mm, 20.67 mm and 27.33 mm; also inhibit the E. coli growth with inhibition zone diameters are 10.08 mm, 12.83 mm and 14.17 mm. The inhibition capability of Agelas sp extract on S. aureus shows that the inhibition zone diameters is 8.83 mm and only appear at 90% of extracts concentration, meanwhile the inhibition capability of Agelas sp extract at concentration 30%, 60% and 90% on E. coli shows diameters of inhibition zone are 7.67 mm, 10.17 mm and 14.17 mm. The inhibition capability of Spheciospongia sp on S. aureus and E. coli only occurred at 90% of extracts concentration with inhibition zone diameters 8.42 mm and 8.75 mm. Based on the results, it can be assumed that extracts of Haliclona sp has a potential antibacterial activity that can be used as a basic ingredients for antibiotic medicine.

scholarly journals Hydrothermal Fabrication of Spindle-Shaped ZnO/Palygorskite Nanocomposites Using Nonionic Surfactant for Enhancement of Antibacterial Activity

Nanomaterials ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 1453 ◽  
Author(s):  
Aiping Hui ◽  
Shuqing Dong ◽  
Yuru Kang ◽  
Yanmin Zhou ◽  
Aiqin Wang
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Low Cost ◽  
Ionic Surfactants ◽  
E Coli ◽  
Antibacterial Performance ◽  
Good Antibacterial Activity ◽  
Controllable Growth ◽  
And Staphylococcus Aureus

In order to improve the antibacterial performance of natural palygorskite, spindle-like ZnO/palygorskite (ZnO/PAL) nanocomposites with controllable growth of ZnO on the surface of PAL were prepared in the presence of non-ionic surfactants using an easy-to-operate hydrothermal method. The obtained ZnO/PAL nanocomposites have a novel and special spindle-shaped structure and good antibacterial activity against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), and are also low cost. The minimum inhibitory concentrations of ZnO/PAL nanocomposites toward E. coli and S. aureus reached 1.5 and 5 mg/mL, respectively.

scholarly journals Binuclear Rhodium(II) Complexes With Selective Antibacterial Activity

1997 ◽  
Vol 4 (2) ◽  
pp. 81-88 ◽  
Author(s):  
Małgorzata Bień ◽  
Tadeusz M. Lachowicz ◽  
Agnieszka Rybka ◽  
Florian P. Pruchnik ◽  
Lilianna Trynda
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Nmr Spectroscopy ◽  
Antibacterial Agents ◽  
Structure And Properties ◽  
E Coli ◽  
Nitrogen Ligands ◽  
And Staphylococcus Aureus ◽  
Low Activity

Binuclear rhodium(II) complexes [Rh2Cl2(μ-OOCR)2(N-N)2] {R = H, Me; N-N = 2,2'-bipyridine (bpy), 1,10-phenanthroline (phen)} and [Rh2(μ-OOCR)2(N-N)2(H2O)2](RCOO)2 (R = Me, Et;) have been synthesized and their structure and properties have been studied by electronic, IR and H1 NMR spectroscopy. Antibacterial activity of these complexes against Escherichia coli and Staphylococcus aureus has been investigated. The most active antibacterial agents against E. coli were [Rh2Cl2(μ-OOCR)2(N-N)2] and [Rh2(μ-OOCR)2(N-N)2(H2O)2](RCOO)2 {R = H and Me} which were considerably more active than the appropriate nitrogen ligands. The complexes show low activity against S. aureus. The activity of the complexes [Rh2(OOCR)2(N-N)2(H2O)2](OOCR)2 against E. coli decreases in the series: R=H≅CH3>C2H5>C3H7≅C4H9. The reverse order was found in the case of S. aureus.

scholarly journals Antibacterial Activity of Lemongrass (Cymbopogon nardus) Ethanolic Extract

2020 ◽  
Vol 6 (2) ◽  
Author(s):  
Waras Nurcholis ◽  
Mira Takene ◽  
Ratna Puspita ◽  
Lisnawati Tumanggor ◽  
Eka Nurul Qomariyah ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antibiotic Resistance ◽  
Infectious Diseases ◽  
Ethanolic Extract ◽  
Leaf Extracts ◽  
E Coli ◽  
Cymbopogon Nardus ◽  
And Staphylococcus Aureus

Infectious diseases are a disease caused by bacteria such as Escherichia coli and Staphylococcus aureus. One way to overcome the disease by using antibiotics. However, wise use can lead to antibiotic resistance, so many bacteria are resistant to antibiotics. Therefore it is necessary to search the natural compounds as antibacterials, one of them by using lemongrass (Cymbopogon nardus). This study aims to determine the effectiveness of leaf extracts, stems, and roots lemongrass as antibacterial. The method used in this research is the disc method. Bacteria used are E. coli and S. aureus. The results show extracts of roots, stems, and leaf has activity against S. aureus, while  E. coli shows no activity as antibacterial, it can be possible because of the different properties of the bacteria.   Keywords: anti-bacteria, lemongrass, Escherichia coli, Staphylococcus aureus

scholarly journals Antibacterial activity of leaves, flowers, and fruits extract of Etlingera elatior from Nagan Raya District, Indonesia against Escherichia coli and Staphylococcus aureus

2021 ◽  
Vol 22 (10) ◽  
Author(s):  
ERNILASARI ERNILASARI ◽  
KAMALLIANSYAH WALIL ◽  
FITMAWATI FITMAWATI ◽  
DEWI INDRIYANI ROSLIM ◽  
ZUMAIDAR ZUMAIDAR ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antibacterial Agents ◽  
Ethanol Extract ◽  
Fruit Extract ◽  
E Coli ◽  
Gas Chromatography Mass Spectroscopy ◽  
And Staphylococcus Aureus ◽  
Etlingera Elatior

Abstract. Ernilasari, Walil K, Fitmawati, Roslim DI, Zumaidar, Saudah, Rayhannisa. 2021. Antibacterial activity of leaves, flowers, and fruits extract of Etlingera elatior from Nagan Raya District, Indonesia against Escherichia coli and Staphylococcus aureus. Biodiversitas 22: 4457-4464. Etlingera elatior is a medicinal plant that has been used by people in Indonesia, especially Acehnese people. Based on its secondary metabolites, E. elatior can be used as antibacterial agents against Gram-positive and Gram-negative. However, to determine the antibacterial activity of E. elatior, the parts of E. elatior that have been used are leaves, flowers, and fruits. This study was aimed to determine the best concentration of ethanol extract of leaves, flowers, and fruits of E. elatior as an inhibitor against Escherichia coli and Staphylococcus aureus. The extract was made by the maceration method with 70% ethanol as a solvent. Antibacterial activity test was carried out by the diffusion agar method using concentrations of 0.5%, 1%, 1.5% and 2%. The results showed that the fruit extract of E. elatior has antibacterial activity with an effective inhibitory zone at a concentration of 2% is 8.4 mm (E. coli) and 2.4 mm (S. aureus). Meanwhile, antibacterial activity the extract of leaves and flowers against E. elatior cannot determine yet. Identification of leaves, flowers and fruits extract of E. elatior using GC-MS (gas chromatography-mass spectroscopy) showed 56 compounds were detected.

scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK DAN FRAKSI ASCIDIAN (Lissoclinum badium) DARI PERAIRAN PULAU MANTEHAGE

PHARMACON ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 897
Author(s):  
Falinry Woran ◽  
Defny S. Wewengkang ◽  
Meilani Jayanti
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bioactive Compounds ◽  
Methanol Fraction ◽  
Agar Method ◽  
Ethanol Extracts ◽  
Escherichia Coli Bacteria ◽  
And Staphylococcus Aureus ◽  
Growth Of Bacteria

ABSTRACTLissoclinum badium is a type of ascidian that contains bioactive compounds. This study aims to determine of presence of antibacterial activity from extracts and fractions of Lissoclinum badium collected from Mantehage Island Manado against Escherichia coli and Staphylococcus aureus bacteria. Samples were extracted by maceration method using 95% ethanol solvent and fractionated using solvents of chloroform, n-hexane and methanol. Antibacterial activity was carried out by the disk diffusion agar method. The results showed that the ethanol extracts an methanol fraction had activity to inhibit the growth of Escherichia coli bacteria with strong category. Meanwhile, against the Staphylococcus aureus the ethanol extracts, chloroform and n-hexane fractions had ability to inhibit the growth of bacteria with weak category.. Keywords: Antibacterial activity, Lissoclinum badium, Escherichia coli, Staphylococcus aureus.  ABSTRAKLissoclinum badium merupakan salah satu jenis tunikata yang memiliki kandungan senyawa bioaktif yang dapat digunakan untuk pengobatan berbagai penyakit. Penelitian ini bertujuan untuk mengetahui adanya aktivitas antibakteri dari ekstrak dan fraksi  Lissoclinum badium yang diperoleh dari Pulau Mantehage Manado terhadap Escherichia coli dan Staphylococcus aureus. Sampel diekstraksi dengan metode maserasi menggunakan pelarut etanol 95% dan fraksinasi menggunakan pelarut metanol, kloroform, dan n-heksan. Aktivitas antibakteri dilakukan dengan  metode difusi agar cakram kertas. Hasil penelitian menunjukkan bahwa ekstrak etanol dan fraksi metanol memiliki aktivitas untuk menghambat bakteri Escherichia coli dan Staphylococcus aureus dengan daya hambat kuat. Sedangkan untuk fraksi kloroform dan fraksi n-heksan memiliki aktivitas untuk menghambat bakteri Staphylococcus aureus saja dengan daya hambat sedang. Kata kunci: Aktivitas antibakteri, Lissoclinum badium, Escherichia coli, Staphylococcus aureus.

scholarly journals UJI EFEK ANTIBAKTERI JAMUR ENDOFIT PADA TUMBUHAN SEREH (Cymbopogon citratus) PADA BAKTERI UJI Staphylococcus aureus DAN Escherichia coli

PHARMACON ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 705
Author(s):  
Fathia Falugah ◽  
Jimmy Posangi ◽  
Paulina V. Y. Yamlean
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Endophytic Fungi ◽  
Pathogenic Bacteria ◽  
Antibacterial Effect ◽  
Cymbopogon Citratus ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Escherichia Coli Bacteria

ABSTRACT Endophytic fungi are fungi that live in plant tissues and do not harm these plants. Endophytic fungi can produce compounds that have the potential as antibacterial. This study aimed to examine the antibacterial effects of endophytic fungi isolated from the lemongrass (Cymbopogon citratus) against Staphylococcus aureus and Escherichia coli bacteria. Lemongrass is used to inhibit or kill pathogenic bacteria because it contains essential oils that function as an antifungal and antibacterial against several pathogenic bacteria such as Staphylococcus aureus and Escherichia coli. The laboratory experiment method tested the antibacterial activity of endophytic fungi isolates obtained from lemongrass (Cymbopogon citratus) against Staphylococcus aureus and Escherichia coli using hole / well method. From the results of the study, four endophytic fungi were isolated from the roots and leaves of lemongrass (Cymbopogon citratus). Antibacterial results showed that endophytic fungi extract isolated from lemongrass plants was more effective in inhibiting Staphylococcus aureus compared to Escherichia coli bacteria. In Staphylococcus aureus bacteria the average inhibitory value is 5-9 mm while in Escherichia coli bacteria the average inhibitory value is only 3 mm. Endophytic fungi from lemongrass roots have a better antibacterial effect compared to endophytic fungi from lemongrass leaves. Conclusion, endophytic fungi isolated from the roots and leaves of lemongrass (Cymbopogon citratus) have an antibacterial effect on the growth of S. aureus bacteria and are not effective against E. coli bacteria. This endophytic fungus isolate is more active against Gram positive (+) bacteria and less active against Gram negative (-) bacteria. Keywords: Lemongrass plants, Endophytic Fungi, Antibacterial Activity, Staphylococcus aureus,  Escherichia coli. ABSTRAK Jamur endofit ialah jamur yang hidup di dalam jaringan tumbuhan dan tidak membahayakan tumbuhan tersebut. Jamur endofit dapat menghasilkan senyawa yang berpotensi sebagai antibakteri. Penelitian ini bertujuan untuk menguji efek antibakteri jamur endofit yang diisolasi dari akar,batang dan daun tumbuhan sereh (Cymbopogon citratus)  terhadap bakteri Staphylococcus aureus dan Escherichia coli. Sereh digunakan untuk menghambat atau membunuh bakteri patogen karena mengandung minyak atsiri yang berfungsi sebagai antijamur dan antibakteri terhadap beberapa bakteri patogen seperti Staphylococcus aureus dan  Escherichia coli.  Metode penelitian secara eksperimen laboratorium menguji aktivitas antibakteri dari isolat jamur endofit  yang diperoleh dari tumbuhan sereh (Cymbopogon citratus) pada bakteri Staphylococcus Aureus dan Escherichia Coli menggunakan metode lubang/sumuran. Dari hasil penelitan diperoleh empat jenis jamur endofit yang diisolasi dari akar dan daun  tumbuhan sereh (Cymbopogon citratus).  Hasil penelitian antibakteri menunjukkan bahwa ekstrak jamur endofit yang diisolasi dari tumbuhan sereh lebih efektif menghambat  bakteri Staphylococcus aureus dibandingkan dengan bakteri Escherichia coli. Pada bakteri  Staphylococcus aureus nilai rata-rata daya hambat yaitu 5-9 mm sedangkan pada bakteri Escherichia coli nilai rata-rata daya hambat yaitu hanya 3 mm. Jamur endofit dari akar sereh memiliki efek antibakteri yang lebih baik dibandingkan dengan jamur endofit dari daun sereh. Kesimpulan, Jamur endofit yang diisolasi dari akar  dan daun sereh (Cymbopogon citratus) memiliki efek antibakteri terhadap pertumbuhan bakteri S. aureus dan tidak efektiv terhadap bakteri E. coli. Isolat jamur endofit ini lebih aktif pada bakteri Gram positif (+) dan kurang  aktif pada bakteri negatif (-). Kata Kunci : Tumbuhan Sereh, Jamur Endofit, Aktivitas Antibakteri, Staphylococcus aureus, Escherichia coli

scholarly journals AKTIVITAS ANTIBAKTERI INFUSA DAUN MUDA MANGROVE Sonneratia alba KERING

2019 ◽  
Vol 7 (2) ◽  
pp. 52
Author(s):  
Yayu Mukhmin Ibrahim ◽  
Verly Dotulong ◽  
Djuhria Wonggo ◽  
Helen Jenny Lohoo ◽  
Roike Iwan Montolalu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Extraction Time ◽  
Gram Positive ◽  
Gram Negative ◽  
E Coli ◽  
Sample Concentration ◽  
Sonneratia Alba ◽  
Escherichia Coli Bacteria

Sonneratia alba mangroves are known to have bioactive compounds such as antibacterial. The purpose of this study was to determine the antibacterial activity of extract of S. alba dried mangrove leaves on Gram positive Staphylococcus aureus and Gram negative Escherichia coli bacteria. Extraction method by infusion, extraction time is 40 and 50 minutes. The extract obtained was then evaporated by the solvent above the water bath then the extracts were made concentrations of 5 and 10%. Antibacterial testing uses the modified Kirby-Bauer method. The highest yield is found in the treatment of 50 minutes infusion extraction time which is 15.6 ± 0.2%. The highest antibacterial activity against S. aureus was found in the treatment of 50 minutes extraction time both for 5% sample concentration of 7.0 mm (medium category) and at a sample concentration of 10% at 8.0 mm (medium category). While the highest antibacterial activity against E. coli bacteria was found in the treatment of 50 minutes extraction time both at 5% sample concentration of 8.0 (medium category) mm and at 10% sample concentration of 8.3 mm (medium category). From these results it can be seen that the S. alba extract of young mangrove leaf infusion has a broad spectrum antibacterial activity because it can inhibit both Gram positive S. aureus and Gram negative E. coli bacteria.Keyword: Mangrove, Sonneratia alba, infusion, rendemen, antibacterial. Mangrove Sonneratia alba diketahui memiliki senyawa bioaktif seperti antibakteri. Tujuan dari penelitian ini untuk mengetahui aktivitas antibakteri ekstrak Infusa daun muda mangrove S. alba kering terhadap bakteri Grampositif Staphylococcus aureus dan bakteri Gram negatif Escherichia coli. Metode ekstraksi dengan cara infusa, lama waktu ekstraksi yaitu40 dan 50 menit. Ekstrak yang didapatkan kemudian dievaporasi pelarutnya diatas penangas air kemudian hasil ekstrak dibuat konsentrasi 5 dan 10%. Pengujian antibakteri menggunakan metode Kirby-Bauer yang dimodifikasi. Rendemen tertinggi terdapat pada perlakuan lama ekstraksi infusa 50 menit yaitu sebesar 15,6±0,2 %. Aktivitas antibakteri tertinggi terhadap bakteri S. aureus terdapat pada perlakuan lama ekstraksi 50 menit baik untuk konsentrasi sampel 5% sebesar 7,0 mm (kategori sedang) dan pada konsentrasi sampel 10% sebesar 8,0 mm (kategori sedang). Sedangkan aktivitas antibakteri tertinggi terhadap bakteri E. coli terdapat pada perlakuan lama ekstraksi 50 menit baik pada konsentrasi sampel 5% sebesar 8,0 mm (kategori sedang) dan pada konsentrasi sampel 10% sebesar 8,3 mm (kategori sedang). Dari hasil ini dapat dilihat bahwa ekstrak infusa daun muda mangrove S. alba mempunyai aktivitas antibakteri dengan spektrum yang luas karena dapat menghambat baik  bakteri Gram positif S. aureus maupun Gram negatif E. coli.Kata kunci: Mangrove, Sonneratia alba, infusa, rendemen, antibakteri.

scholarly journals POTENSI ANTIBAKTERI BINTANG LAUT DARI PERAIRAN PANTAI KELURAHAN TONGKAINA MANADO

2019 ◽  
Vol 7 (3) ◽  
pp. 167
Author(s):  
Delpris Piter ◽  
Esther D Angkouw ◽  
Fitje Losung
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Streptococcus Agalactiae ◽  
Antibacterial Agents ◽  
Sea Water ◽  
Diffusion Method ◽  
E Coli ◽  
Linckia Laevigata ◽  
And Staphylococcus Aureus

Starfish is one of the species of the asteroidean class, and is grouped into phylum echinoderms. Echinoderms consist of approximately 6000 species and all live in sea water. Starfish is one source bioactive compounds. Active compounds from starfish have been known to have activities antioxidant, antibacterial, anti-inflammatory, antifungal and immunostimulatory, there are also blue starfish that are potential as antitumor and antibacterial agents. This study aims to obtain crude extracts from several types of starfish, and testing the antibacterial activity of crude starfish extracts against bacteria Streptococcus agalactiae, Escherichia coli and  Staphylococcus aureus. Test the antibacterial activity of crude starfish extracs Protoreaster nodosus, Achaster tipycus, dan Linckia laevigata against bacteria E. coli, S. aureus, dan S. agalactiae use the agar diffusion method by means of a well.  L. l extract  can inhibit bacteria e. coli at an extract concentration of 1000 mg/ml with a diameter of inhibitory zone 11.0 mm, whereas at a concentration of 750 mg/ml the test bacteria can still grow on the bacteria S. agalactiae and S. aureus can still grow even though the extract has be given  L. l Keywords: Potential Starfish, Antibacterial Activity, Staphylococcus aureus, Escherichia coli, and Streptococcus agalactiae Bintang laut merupakan salah satu spesies dari kelas Asteroidea, dan dikelompokkan kedalam filum Echinodermata. Filum Echinodermata  terdiri atas kurang lebih 6000 spesies dan semuanya hidup di air laut. Bintang laut  merupakan salah satu sumber penghasil senyawa bioaktif. Senyawa aktif dari bintang laut telah diketahui memiliki aktivitas antioksidan, antibakteri, antiinflamasi, antifungi dan imunostimulator, ada juga bintang laut biru yang potensial sebagai antitumor dan agen antibakteri. Penelitian ini bertujuan untuk mendapatkan ekstrak kasar dari beberapa jenis bintang laut, dan menguji aktivitas antibakteri ekstrak kasar bintang laut terhadap bakteri Streptococcus agalactiae, Escherichia coli dan  Staphylococcus aureus. Uji aktivitas antibakteri dari ekstrak kasar bintang laut Protoreaster nodosus, Achaster tipycus, dan Linckia laevigata terhadap bakteri E. coli, S. aureus, dan S. agalactiae mengggunakan metode difusi agar dengan cara sumur. Ekstrak L. l mampu menghambat bakteri E.coli pada konsentrasi ekstrak 1.000 mg/ml dengan diameter zona hambat 11.0 mm, sedangkan pada konsentrasi 750 mg/ml bakteri uji masih bisa bertumbuh pada bakteri S. agalactiae dan S. aureus masih dapat bertumbuh walaupun telah diberikan ekstrak L. l.Kata Kunci : Potensi Bintang Laut, Aktivitas Antibakteri, Streptococcus agalactiae, Escherichia coli and  Staphylococcus aureus

Sign in / Sign up

Export Citation Format

Share Document