Characteristics analysis of Orf virus from goats to human

2018 ◽  
pp. 1-3
Keyword(s):  
Infectious Disease ◽  
Phylogenetic Analysis ◽  
Electron Microscope ◽  
Gene Sequence ◽  
Clinical Symptoms ◽  
Orf Virus ◽  
Virus Infections ◽  
B2l Gene ◽  
Characteristics Analysis ◽  
Contagious Pustular Dermatitis

Contagious pustular dermatitis (orf) is an exanthemous disease affecting sheep and goats primarily. As a zoonotic infectious disease caused by parapoxvirus, orf should be managed not only in animals but also in humans. In this study, the typical orf clinical symptoms in goat and humans were observed. Human and goat samples were drawn. The Orf virus (orfv) was identified using an electron microscope, and PCR was used to amplify the target for B2L gene sequence. Molecular analysis of other B2L gene sequences downloaded from GenBank was performed by Mega4 soft. Results indicated that a 21-year old girl who worked in a goat farmcontacted orfv infection from infected goats directly. Eleven amino acid (AA) mutations were detected in the goat orfv transmitted to human orfv. Phylogenetic analysis showed that human orfv in this study was closely related genetically to FJ-SJ2 (KC568397), which was isolated from Fujian province 2012. The results facilitate the development of programs to control Orf virus infections not only in goats but also in humans.

scholarly journals Comparison and phylogenetic analysis based on the B2L gene of orf virus from goats and sheep in China during 2009-2011

2013 ◽  
Vol 159 (6) ◽  
pp. 1475-1479 ◽  
Author(s):  
Keshan Zhang ◽  
Yongjie Liu ◽  
Hanjin Kong ◽  
Youjun Shang ◽  
Xiangtao Liu
Keyword(s):  
Phylogenetic Analysis ◽  
Orf Virus ◽  
B2l Gene

scholarly journals Phylogenetic analysis of B2L gene of Egyptian orf virus from naturally infected sheep

VirusDisease ◽  
2015 ◽  
Vol 26 (3) ◽  
pp. 147-150 ◽  
Author(s):  
Mohamed El-Tholoth ◽  
Yasser F. Elnaker ◽  
Gamal Shiha
Keyword(s):  
Phylogenetic Analysis ◽  
Infected Sheep ◽  
Orf Virus ◽  
B2l Gene

scholarly journals Phylogenetic analysis of eight sudanese camel contagious ecthyma viruses based on B2L gene sequence

2015 ◽  
Vol 12 (1) ◽  
Author(s):  
Abdelmalik I. Khalafalla ◽  
Ibrahim M. El-Sabagh ◽  
Khalid A. Al-Busada ◽  
Abdullah I. Al-Mubarak ◽  
Yahia H. Ali
Keyword(s):  
Phylogenetic Analysis ◽  
Gene Sequence ◽  
Contagious Ecthyma ◽  
B2l Gene

Coxiella burnetii in cattle and their human contacts in a gaushala (cattle shelter) from India and its partial com1 gene sequence-based phylogenetic analysis

2021 ◽  
pp. 1-10
Author(s):  
Jay Prakash Yadav ◽  
Satya Veer Singh Malik ◽  
Pankaj Dhaka ◽  
Ashok Kumar ◽  
Manesh Kumar ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Coxiella Burnetii ◽  
Gene Sequence

scholarly journals Case of Human Infestation with Dermanyssus gallinae (Poultry Red Mite) from Swallows (Hirundinidae)

Pathogens ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 299
Author(s):  
Georgios Sioutas ◽  
Styliani Minoudi ◽  
Katerina Tiligada ◽  
Caterina Chliva ◽  
Alexandros Triantafyllidis ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Clinical Symptoms ◽  
Skin Lesions ◽  
Coi Gene ◽  
Dermanyssus Gallinae ◽  
Poultry Red Mite ◽  
Human Form ◽  
Bayesian Phylogenetic Analysis

Dermanyssus gallinae (the poultry red mite, PRM) is an important ectoparasite in the laying hen industry. PRM can also infest humans, causing gamasoidosis, which is manifested as skin lesions characterized by rash and itching. Recently, there has been an increase in the reported number of human infestation cases with D. gallinae, mostly associated with the proliferation of pigeons in cities where they build their nests. The human form of the disease has not been linked to swallows (Hirundinidae) before. In this report, we describe an incident of human gamasoidosis linked to a nest of swallows built on the window ledge of an apartment in the island of Kefalonia, Greece. Mites were identified as D. gallinae using morphological keys and amplifying the Cytochrome C oxidase subunit I (COI) gene by PCR. Bayesian phylogenetic analysis and median-joining network supported the identification of three PRM haplogroups and the haplotype isolated from swallows was identical to three PRM sequences isolated from hens in Portugal. The patient was treated with topical corticosteroids, while the house was sprayed with deltamethrin. After one week, the mites disappeared and clinical symptoms subsided. The current study is the first report of human gamasoidosis from PRM found in swallows’ nest.

scholarly journals Denitratisoma oestradiolicum gen. nov., sp. nov., a 17β-oestradiol-degrading, denitrifying betaproteobacterium

2006 ◽  
Vol 56 (7) ◽  
pp. 1547-1552 ◽  
Author(s):  
Michael Fahrbach ◽  
Jan Kuever ◽  
Ruth Meinke ◽  
Peter Kämpfer ◽  
Juliane Hollender
Keyword(s):  
Phylogenetic Analysis ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Sequence ◽  
Municipal Wastewater ◽  
Treatment Plant ◽  
16S Rrna Gene Sequence ◽  
Physiological Data ◽  
Rrna Gene ◽  
Rrna Gene Sequence

A Gram-negative, motile, denitrifying bacterium (strain AcBE2-1T) was isolated from activated sludge of a municipal wastewater treatment plant using 17β-oestradiol (E2) as sole source of carbon and energy. Cells were curved rods, 0.4–0.8×0.8–2.0 μm in size, non-fermentative, non-spore-forming, oxidase-positive and catalase-negative. E2 was oxidized completely to carbon dioxide and water by reduction of nitrate to a mixture of dinitrogen monoxide and dinitrogen, with the intermediate accumulation of nitrite. Electron recoveries were between 90 and 100 %, taking assimilated E2 into account. With nitrate as the electron acceptor, the bacterium also grew on fatty acids (C2 to C6), isobutyrate, crotonate, dl-lactate, pyruvate, fumarate and succinate. Phylogenetic analysis of its 16S rRNA gene sequence revealed that strain AcBE2-1T represents a separate line of descent within the family Rhodocyclaceae (Betaproteobacteria). The closest relatives are the cholesterol-degrading, denitrifying bacteria Sterolibacterium denitrificans DSM 13999T and strain 72Chol (=DSM 12783), with <93.9 % sequence similarity. The G+C content of the DNA was 61.4 mol%. Detection of a quinone system with ubiquinone Q-8 as the predominant compound and a fatty acid profile that included high concentrations of C16 : 1 ω7c/iso-C15 : 0 2-OH and C16 : 0, in addition to C18 : 1 ω7c and small amounts of C8 : 0 3-OH, supported the results of the phylogenetic analysis. On the basis of 16S rRNA gene sequence data in combination with chemotaxonomic and physiological data, strain AcBE2-1T (=DSM 16959T=JCM 12830T) is placed in a new genus Denitratisoma gen. nov. as the type strain of the type species Denitratisoma oestradiolicum gen. nov., sp. nov.

Mixed polysaccharides derived from shiitake mushroom, Poriacocos, Ginger and Tangerine peel prevent the H1N1 virus infections in mice

2021 ◽  
Author(s):  
Diqi Yang ◽  
Minghua Hu ◽  
Hongmei Zhu ◽  
Jianguo Chen ◽  
Dehai Wang ◽  
...  
Keyword(s):  
Influenza A ◽  
Clinical Symptoms ◽  
H1n1 Virus ◽  
Global Public Health ◽  
Virus Infections ◽  
Shiitake Mushroom ◽  
Virus Challenge ◽  
Influenza A H1n1 ◽  
Public Health Challenges ◽  
Cellular Immune

Abstract The pandemic influenza A (H1N1) virus spread globally and posed one of the most serious global public health challenges. The traditional Chinese medicine is served as a complementary treatment strategy with vaccine immunization. Here, we demonstrated the mixed polysaccharides (MPs) derived from shiitake mushroom, poriacocos, ginger and tyangerine peel prevent the H1N1 virus infections in mice. MPs pretreatment attenuated H1N1 virus-induced weight loss, clinical symptoms and death. The lymphocytes detection results showed the CD3+, CD19+ and CD25+ cell proportions were up-regulated in thymus under MPs pretreatment. Besides, MPs pretreatment reduced the inflammatory cell infiltration and increased the cell proportions of CD19+, CD25+ and CD278+ in lung. However, MPs treatment have no effective therapeutic effect after H1N1 virus challenge. The current study suggested that pretreatment with MPs could attenuate H1N1 virus-induced lung injury and up-regulate humoral and cellular immune responses in non- immunized mice.

scholarly journals Fine Structure of Cellular Inclusions in Measles Virus Infections

1959 ◽  
Vol 6 (3) ◽  
pp. 379-382 ◽  
Author(s):  
Frances Kallman ◽  
John M. Adams ◽  
Robley C. Williams ◽  
David T. Imagawa
Keyword(s):  
Fine Structure ◽  
Electron Microscope ◽  
Living Cell ◽  
Measles Virus ◽  
Cytoplasmic Inclusion ◽  
Virus Infections ◽  
Cellular Inclusions ◽  
Staining Methods ◽  
Cellular Components ◽  
Inclusion Material

Cells which are infected with measles virus have been known for some time to contain inclusion material that is distinguishable from normal cellular components by application of traditional staining methods and observation in the light microscope. The fine structure of the inclusion material contained in HeLa cells infected with Edmonston strain of measles virus has been examined in the electron microscope. Two steps have been found necessary in this study: (1) the recognition by phase-contrast microscopy of the living cell of bodies that are defined as inclusion material when the cells are classically stained; and (2) the recognition in the electron microscope of inclusion-body material that had previously been identified in the living cell. The fine structure of the nuclear and cytoplasmic inclusion material in osmium-treated cells was found to consist mainly of randomly arrayed filaments of low electron density. Dense, highly ordered arrays of filaments were found near the center of the nuclear inclusions, sometimes as a two-dimensional, nearly orthogonal arrangement. If the size of the measles virus is taken to be around 100 mµ in diameter, the strands seen in the inclusions cannot be fully formed virus.

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