Preparation and application of tilmicosin-templated magnetic surface molecularly imprinted polymer

2019 ◽  
Vol 37 (9) ◽  
pp. 932
Author(s):  
Zhangyueyi XIA ◽  
Bingcheng YANG ◽  
Feifang ZHANG ◽  
Xinmiao LIANG
Keyword(s):  
Molecularly Imprinted Polymer ◽  
Magnetic Surface ◽  
Imprinted Polymer ◽  
Molecularly Imprinted

scholarly journals Using Chiral Magnetic Surface Molecularly Imprinted Polymers for Chiral Separation of Ofloxacin

2020 ◽  
Author(s):  
ZHAO CHEN ◽  
Wenzong Lu ◽  
Mengxiang Yang
Keyword(s):  
Molecularly Imprinted Polymer ◽  
Optical Rotation ◽  
High Specificity ◽  
Cost Effective ◽  
Magnetic Surface ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Chiral Reagent ◽  
Chiral Compounds ◽  
Cost Effective Method

Abstract BackgroundOfloxacin is an important drug with anti-bacterial effects, and (–)-ofloxacin has been shown to have better anti-bacterial activity than racemic ofloxacin. But chiral resolution of compounds is a major challenge worldwide. Many methods do not show high specificity to target molecules, causing errors in the separation of these pharmaceutical molecules, thus affecting the purity of drugs.MethodThe paper mainly research on a method for quick resolution of ofloxacin using chiral magnetic surface molecular imprinting polymers (chiral MMIP). Fe3O4 magnetic nanoparticles coated with silica is acted as the substrate. Chiral reagent is N-octyl-D-glucosamine as monomer, with ofloxacin as the template molecules to prepare magnetic surface molecularly imprinted polymer. The chiral polymer can be combined with (+)-ofloxacin, eventually (-)-ofloxacin was isolated. ResultsFinally by using extermal magnetic field and transmission electron microscopy (TEM), the character of magnetic surface molecularly imprinted polymer is tested, and The product was tested by ultraviolet spectrum and optical rotation tests and was confirmed to be (-)-ofloxacin. ConclusionA simple, rapid, and cost-effective method for separating chiral compounds was established.

Liquid Chromatography Analysis of Clozapine in Rat Brain Tissue, Using its Molecularly Imprinted Polymer after Administration of Toxic Dose of Drug and Lipid Emulsion Therapy

2019 ◽  
Vol 15 (3) ◽  
pp. 251-257
Author(s):  
Bahareh Sadat Yousefsani ◽  
Seyed Ahmad Mohajeri ◽  
Mohammad Moshiri ◽  
Hossein Hosseinzadeh
Keyword(s):  
Rat Brain ◽  
Brain Tissue ◽  
Molecularly Imprinted Polymer ◽  
Lipid Emulsion ◽  
Limit Of Detection ◽  
Imprinted Polymer ◽  
Toxic Dose ◽  
Molecularly Imprinted ◽  
The Brain ◽  
Rat Brain Tissue

Background:Molecularly imprinted polymers (MIPs) are synthetic polymers that have a selective site for a given analyte, or a group of structurally related compounds, that make them ideal polymers to be used in separation processes.Objective:An optimized molecularly imprinted polymer was selected and applied for selective extraction and analysis of clozapine in rat brain tissue.Methods:A molecularly imprinted solid-phase extraction (MISPE) method was developed for preconcentration and cleanup of clozapine in rat brain samples before HPLC-UV analysis. The extraction and analytical process was calibrated in the range of 0.025-100 ppm. Clozapine recovery in this MISPE process was calculated between 99.40 and 102.96%. The limit of detection (LOD) and the limit of quantification (LOQ) of the assay were 0.003 and 0.025 ppm, respectively. Intra-day precision values for clozapine concentrations of 0.125 and 0.025 ppm were 5.30 and 3.55%, whereas inter-day precision values of these concentrations were 9.23 and 6.15%, respectively. In this study, the effect of lipid emulsion infusion in reducing the brain concentration of drug was also evaluated.Results:The data indicated that calibrated method was successfully applied for the analysis of clozapine in the real rat brain samples after administration of a toxic dose to animal. Finally, the efficacy of lipid emulsion therapy in reducing the brain tissue concentration of clozapine after toxic administration of drug was determined.Conclusion:The proposed MISPE method could be applied in the extraction and preconcentration before HPLC-UV analysis of clozapine in rat brain tissue.

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