scholarly journals The Effects of Sambiloto Leaf Extract (Andrographis peniculata) on Blood Sugar Regulation: an In Vivo Study

2020 ◽  
Vol 1 (1) ◽  
pp. 1-8
Author(s):  
Lusia Hayati ◽  
Rachmat Hidayat
Keyword(s):  
Blood Glucose ◽  
Blood Sugar ◽  
Leaf Extract ◽  
Negative Control ◽  
Control Group ◽  
Diabetes Group ◽  
White Rats ◽  
Sugar Regulation ◽  
Sugar Levels

Sambiloto (Andrographis peniculata) is one of the most common plants inIndonesia. Sambiloto contains quite varied secondary metabolites, where thisplant is rich in flavonoids, alkaloids, terpenes and glycosides. This study aimsto assess the effect of Sambiloto (Andrographis peniculata) leaf extract on bloodsugar levels and the expression of GLUT4 protein in muscle tissue whichindicates the potential of the test extract's ability to improve blood glucose intaketo cells so that it can maintain blood sugar regulation. The process of extractingsambiloto is carried out by maceration in which 500 grams of simplicia aremacerated with 96% ethanol for 72 hours. After 1 week of adaptation, the micewere randomly divided into the following six groups, each containing 5 animals:Normal control group, diabetes group (negative control), diabetes + metformingroup (Met; 45 mg/kg), Diabetes + ES group (50 mg/kg), diabetes + ES group(100 mg/kg) and diabetes + ES group (200 mg/kg). The treatment with sambilotoextract was able to reduce blood sugar levels significantly, were at the ES 100and 200 mg/kg BW doses it was able to reduce blood sugar levels to reach thetarget below 200 mg/kg BW. The dominant flavonoids in Sambiloto leaf extractare believed to be responsible for the effect of blood glucose regulation. Inconclusion, Sambiloto extract affects lowering blood sugar levels in diabetesmellitus white rats by increasing glucose intake to cells and tissues.

scholarly journals Effect of Bay Leaf Extract (Syzygium polyanthum) on Blood Sugar Regulation via GLUT4 Protein Regulation in White Rat Muscle Tissue Induced Aloxan

2020 ◽  
Vol 1 (1) ◽  
pp. 24-31
Author(s):  
Rachmat Hidayat ◽  
Lusia Hayati
Keyword(s):  
Diabetes Mellitus ◽  
Blood Sugar ◽  
Muscle Tissue ◽  
Leaf Extract ◽  
Negative Control ◽  
Diabetes Group ◽  
White Rats ◽  
Target Blood Glucose ◽  
Sugar Regulation ◽  
Sugar Levels

Diabetes mellitus is a chronic condition that disturbs the body's bloodsugar regulation. Bay leaves contain entirely various secondary metabolites,where this plant is rich in flavonoids, alkaloids, terpenes and glycosides. Thisstudy aims to assess the effect of bay leaf extract (Syzygium polyanthum) onblood sugar levels and the expression of GLUT4 protein in muscle tissue. A totalof 30 white rats (Rattus norvegicus) Wistar strain obtained from the EurekaResearch Laboratory (Palembang, Indonesia) weighing between 200 - 250 grams.Bay leaf simplicia was obtained from the Tawangmangu Herbal Research Center,Karanganyar, Indonesia. After 1 week of adaptation, the mice were randomlydivided into the following six groups, each containing 5 animals: Normal controlgroup, diabetes group (negative control), diabetes + metformin group (Met; 45mg / kg), Diabetes + BLE (75 mg/kg), diabetes + BLE group (150 mg / kg) anddiabetes + BLE group (300 mg/kg). Alloxan-induced white rats showed a verysignificant increase in blood sugar levels, where the use of the drug metforminwas able to reduce blood sugar levels significantly even though they had notreached the target blood glucose target of less than 200 mg / dL. The treatmentwith bay leaf extract was able to reduce blood sugar levels significantly. Theadministration of metformin drugs or bay leaf extract showed the ability toincrease the level of GLUT4 protein. In conclusion, bay leaf extract affectsreducing blood sugar levels in diabetes mellitus white rats by increasing glucoseintake to cells and tissues.

scholarly journals UJI AKTIVITAS ANTIDIABETES KOMBINASI EKSTRAK ETANOL DAUN ECENG GONDOK (Eichhornia crassipes (Mart.)Solms) DAN DAUN SINTRONG (Crassocephalum crepidioides (Benth.) S. Moore) PADA MENCIT

2021 ◽  
Vol 4 (1) ◽  
pp. 44-53
Author(s):  
Andri Priyoherianto ◽  
◽  
Panji Ratih Suci ◽  
Putri Rizki Cahya Fatimah ◽  
Atik Nur Wijayanti
Keyword(s):  
Blood Glucose ◽  
Blood Sugar ◽  
Water Hyacinth ◽  
Leaf Extract ◽  
Positive Control ◽  
Negative Control ◽  
Glucose Levels ◽  
Normal Limits ◽  
Blood Glucose Levels ◽  
Sugar Levels

Diabetes mellitus is a chronic metabolic disorder that occurs due to destruction of pancreatic ? cells characterized by blood glucose levels that exceed normal limits. The purpose of this study was to determine the therapeutic effect of the combination of water hyacinth leaf extract and Sintrong leaf in reducing blood glucose levels in alloxan-induced experimental animals. Water hyacinth leaf powder and Sintrong leaves were extracted using the maceration method with 70% ethanol as a solvent. A total of 25 mice were divided into 5 groups, namely negative control CMC-Na 0.5%, positive control glibenclamide 0.00065 mg / g BW, water hyacinth leaf extract 17.5 mg / kg BW, Sintrong leaf extract 10.5 mg/ kg BW and a combination of water hyacinth leaf extract 17.5 mg / kg BW and sintrong leaf 10.5 mg / kg BW. The results showed that a single extract of water hyacinth and Sintrong leaves was able to reduce blood sugar levels for 7 days. The combination dose was effective in reducing blood sugar levels with a combination dose of 17.5 mg/ kg water hyacinth leaf extract in combination for 7 days.

scholarly journals The Difference in Effect of Arabica Coffee Gayo Beans and Leaf (Coffea Arabica Gayo) Extract on Decreasing Blood Sugar Levels in Healthy Mice

2019 ◽  
Vol 7 (20) ◽  
pp. 3363-3365
Author(s):  
Sake Juli Martina ◽  
Pradeepa A. P. Govindan ◽  
Arlinda Sari Wahyuni
Keyword(s):  
Blood Glucose ◽  
Blood Sugar ◽  
Leaf Extract ◽  
Alternative Therapy ◽  
Postprandial Blood Glucose ◽  
Control Group ◽  
Arabica Coffee ◽  
Before And After ◽  
The Difference ◽  
Sugar Levels

BACKGROUND: High incidence rate of diabetes mellitus (DM) and increased incidence of complications from DM as well as the use of less effective antidiabetic drugs and high financing to treat demands alternative therapy. AIM: This study was conducted to determine the difference in the effect of Arabica coffee gayo bean and leaf extract on blood sugar levels in healthy mice before and after the intervention of extract and fasting and postprandial blood glucose level after consumption of glucose. METHODS: This research is an experimental research study. The study used experimental animals which were divided into 8 groups which are the control group (Aquadest), group given Acarbose, the group given the Arabica coffee gayo bean extract with dosage of 50 mg, 100 mg and 200 mg in healthy mice, the group given the Arabica coffee gayo leaf extract with dosage of 30 mg, 60 mg and 120 mg in healthy mice. RESULTS: The results of the study obtained are before and after the intervention of extract showed the difference of p= 0.523 and after consumption of glucose the fasting and postprandial result showed the difference of p = 0.005. CONCLUSION: The use of Arabica coffee gayo bean and leaf extract showed a high decrease in blood glucose levels (BGL) compared to the control group.

scholarly journals UJI EFEKTIVITAS ANTIDIABETES KOMBINASI EKSTRAK DAUN MENIRAN (Phyllanthus niruri L.) Dan KELOPAK BUNGA ROSELLA (Hibiscus sabdariffa L.) PADA TIKUS JANTAN PUTIH

2019 ◽  
Vol 2 (1) ◽  
pp. 24-28
Author(s):  
Herviani Sari ◽  
Vera Estefania Kaban ◽  
Friska Raulina Situmorang ◽  
Firdaus Fahdi
Keyword(s):  
Blood Glucose ◽  
Leaf Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
High Dose ◽  
Glucose Levels ◽  
White Rats ◽  
Blood Glucose Levels

Purpose: To determine the effect of decreasing blood glucose levels in white rats using a combination of meniran and rosella compared to glibenclamide. Method: This research was carried out experimentally. Simplicia of meniran leaves and rosella macerated using 80% ethanol. The research used 21 rats that were induced by alloxan and divided into 7 groups and all compared using glibenclamide.Group 1 (negative control) CMC Na 1%, group 2 (positive control) glibenclamide dose 0.45 mg/kgMB, group 3 single roselle extract dose 130 mg/kgMB, group 4 single meniran leaf extract dose 200 mg/kgMB, group 5 combination of meniran leaf extract dose of 100 mg/kgMB and rosella extract dose of 65 mg/kgMB, group 6 combination of meniran leaf extract dose of 200 mg/kgMB and rosella extract dose of 130 mg/kgMB, and group 7 meniran leaf extract combination dose 400 mg/kgMB and rosella extract dose 195 mg/kgMB. Result: The results showed that rats had hyperglycemia after being induced by alloxan.Data were analyzed using one way ANOVA method followed by LSD and tukeys' B post hoct test. Having a difference in the decrease in blood glucose levels between the positive control group and the five doses of meniran extract and rosella gave a significant effect compared to the negative control group did not have a significant effect in reducing blood glucose levels. Conclusion: Single meniran extract and high-dose combination extract are more effective than glibenclamide.

UJI EFEKTIVITAS ANTIDIABETES KOMBINASI EKSTRAK DAUN MENIRAN (Phyllanthus niruri L.) dan KELOPAK BUNGA ROSELLA (Hibiscus sabdariffa L.) pada TIKUS JANTAN PUTIH SEBAGAI HEWAN PERCOBAAN

2019 ◽  
Vol 2 (1) ◽  
pp. 19-23
Author(s):  
Herviani Sari ◽  
Vera Estefania Kaban ◽  
Friska Raulina Situmorang ◽  
Firdaus Fahdi
Keyword(s):  
Blood Glucose ◽  
Leaf Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
High Dose ◽  
Glucose Levels ◽  
White Rats ◽  
Blood Glucose Levels

Background : Diabetes mellitus (DM) is a chronic disease caused by the body's inability to produce the hormone insulin or due to ineffective use of insulin production. Diabetes has become a global problem, DM prevalence of 4.6%, estimated in 2000 amounted to 5.6 million. One of the plants that can reduce blood glucose levels is meniran and rosella which are found in Indonesia. Objective : To determine the effect of decreasing blood glucose levels in white rats using a combination of meniran and rosella compared to glibenclamide.Method : This research was carried out experimentally. Simplicia of meniran leaves and rosella macerated using 80% ethanol. The research used 21 rats that were induced by alloxan and divided into 7 groups and all compared using glibenclamide.Group 1 (negative control) CMC Na 1%, group 2 (positive control) glibenclamide dose 0.45 mg / kgBB, group 3 single roselle extract dose 130 mg / kgBB, group 4 single meniran leaf extract dose 200 mg / kgBB, group 5 combination of meniran leaf extract dose of 100 mg / kgBB and rosella extract dose of 65 mg / kgBB, group 6 combination of meniran leaf extract dose of 200 mg / kgBB and rosella extract dose of 130 mg / kgBB, and group 7 meniran leaf extract combination dose 400 mg / kgBB and rosella extract dose 195 mg / kgBB. Result : The results showed that rats had hyperglycemia after being induced by alloxan.Data were analyzed using one way ANOVA method followed by LSD and tukeys' B post hoct test. Having a difference in the decrease in blood glucose levels between the positive control group and the five doses of meniran extract and rosella where p <0.05 gave a significant effect compared to the negative control group p> 0.05 did not have a significant effect in reducing blood glucose levels. Conclusion : Single meniran extract and high-dose combination extract are more effective than glibenclamide.

scholarly journals EFEKTIVITAS ANTIDIABETES EKSTRAK DAUN RAMBUSA (Passiflora foetida L.) PADA MENCIT (Mus musculus) DENGAN INDUKSI GLUKOSA

2015 ◽  
Vol 1 (2) ◽  
pp. 99-104
Author(s):  
Khildah Khaerati ◽  
Ihwan Ihwan ◽  
Musdalifah S Maya
Keyword(s):  
Blood Sugar ◽  
Leaf Extract ◽  
Drug Research ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Antidiabetic Drug ◽  
Passiflora Foetida ◽  
Sugar Levels

Passiflora foetida leaves used by the society for generations as a natural antidiabetic drug research aims to test the effectiveness of the leaf extract of Passiflora foetida with variations of a dose of 250 mg / kg, 500 mg / kg, and 750 mg / kg in lowering blood sugar levels in mice. Passiflora foetida leaves were extracted by sokhletasi method with 70% ethanol, the extract was tested on 15 mice, the test animals were randomized into 5 groups consist of negative control group given 0.5% Na-CMC, positive control group was given glibenclamide 0, 65 mg / kg, and the treatment group were given the leaf extract of Passiflorafoetida with variations of each dose of 250 mg / kg, 500 mg / kg, 750 mg / kg. To increase blood sugar levels in mice induced glucose 50%. Measurement of blood sugar levels in mice were performed using an easy touch glucometer and blood is taken via the lateral tail vein. The results showed that the percentage of blood sugar levels drop to a negative control is 13.1%, for a dose of 250 mg / kg body weight of 29.52%, for a dose of 500 mg / kg was 38.79%, to dosis750 mg / kg was 49, 21%, and 48.1% for the positive control. The results showed that the leaf extract of Passiflorafoetida at a dose of 750 mg / kg is most effective as an antidiabetic

scholarly journals Effect of Giving Extract of Lannea Coromandelica (Lannea coromandelica (Houttuyn) Merrill) Leaf to Decrease the Blood Sugar Level of Mice (Mus musculus)

2019 ◽  
Vol 8 (4) ◽  
pp. 223-229
Author(s):  
Moh. Husaini ◽  
Supriadi Supriadi ◽  
Minarni R. Jura
Keyword(s):  
Blood Glucose ◽  
Blood Sugar ◽  
Blood Sugar Level ◽  
Leaf Extract ◽  
Negative Control ◽  
Animal Testing ◽  
Leaf Sample ◽  
Lannea Coromandelica ◽  
Sugar Levels ◽  
Tree Leaf

The Indian ash tree leaf (Lannea coromandelica (Houttuyn) Merrill) contains natural ingredients that can reduce blood glucose levels. This study aimed to determine the concentration of the Indian ash tree leaf extract which is the most effective to reduce blood sugar levels of mice. The leaf sample of the Indian ash tree plant was obtained from Talise sub-district, Mantikulore district, Palu, Central Sulawesi. The preparation of the extract was conducted by the maceration method. The animal testing used were 20 male mice that were induced by EDTA (ethylene diamine tetraacetic acid). Mice were divided into 5 groups as random with different treatments. Treatments I, II, and III were given an extract of 10, 20, and 40%, respectively, treatment IV was given Na-CMC as a negative control (-), and treatment V was given glibenclamide suspension as a positive control (+). The data obtained were analyzed by statistical analysis of variance analysis (ANOVA) and were continued by the Duncan test. The results showed that the Indian ash tree leaf extract can reduce the blood sugar levels of mice. The mean decreased blood glucose level at treatment I was 40.00 mg/dL, treatment II was 47.75 mg/dL, treatment III was 54.75 mg/dL, treatment IV was 1.75 mg/dL, and treatment V was 49.00 mg/dL. The conclusion of this research was that the Indian ash tree leaf extract could reduce the blood sugar level of mice with an effective concentration of 20% with α = 0.05.

scholarly journals PENGARUH PEMBERIAN EKSTRAK DAUN SIRSAK (Annona Muricata L.) TERHADAP KADAR GULA DARAH TIKUS WISTAR (Rattus norvegicus) YANG DIINDUKSI ALLOXAN

2014 ◽  
Vol 2 (1) ◽  
Author(s):  
Febbyola S. Moniaga
Keyword(s):  
Blood Glucose ◽  
Blood Sugar ◽  
Wistar Rat ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Annona Muricata ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Sugar Levels

Abstract: People sure that Soursop leaves (A.muricata L.) has a lot of advantage for health, one example is to reduced blood glucose levels. The objectives of this research were to study the effectiveness of administration of soursop leaves extract (A.muricata L.) on blood glucose levels of wistar rat (R.norvegicus) induced by alloxan. The subjects in these research were male wistar rat with total of 18 samples divided into 6 groups as follows negative control group and 5 hyperglycemic groups induced by alloxan with dose 110 mg/kg bodyweight of rat. Hyperglycemic rats administered by soursop leaves extract dosing 1000 mg, 2000 mg, and 5000 mg/kg bodyweight of rat, positive control groups administered by novomix flexpen, and the last one only administered by alloxan. Data taken from measurement of blood sugar on first day, second day, and third day on minutes 0,30,60,90, and 120. The result showed reduction of alloxan-induced wistar rat’s blood sugar levels on administration of soursop leaves extract. Keyword: Annona Muricata L, Soursop leaves, Blood sugar levels, Alloxan.       Abstrak: Daun sirsak (A.muricata L.) diyakini masyarakat memiliki banyak manfaat bagi kesehatan salah satunya menurunkan kadar gula darah. Penelitian ini bertujuan untuk menguji pengaruh pemberian ekstrak daun sirsak (A.muricata L.) terhadap kadar gula darah tikus Wistar (R.norvegicus) yang diinduksi alloxan. Subjek penelitian berupa tikus Wistar jantan berjumlah 18 ekor yang dibagi dalam 6 kelompok, kelompok kontrol negatif dan 5 kelompok tikus Wistar hiperglikemik akibat pemberian alloxan dengan dosis 110 mg/kg berat badan tikus. Tikus hiperglikemik diberi ekstrak daun sirsak dosis 1000, 2000, dan 5000 mg/kg BB tikus,  kelompok kontrol positif yang diberi novomix flexpen, dan 1 kelompok hanya diberi alloxan. Data diperoleh dari pemeriksaan kadar gula darah pada semua kelompok tikus Wistar pada hari pertama, hari kedua , hari ketiga pada menit ke-0 ,30, 60, 90, dan menit ke-120. Hasil penelitian menunjukkan bahwa pemberian ekstrak daun sirsak mempunyai efek terhadap penurunan kadar gula darah tikus Wistar yang telah diinduksi alloxan. Kata kunci: Annona Muricata L., Daun Sirsak, Kadar Gula Darah, Alloxan.

scholarly journals Effect of Mangkokan (Polyscias scutellaria) Leaf Extract on Blood Sugar Levels in Alloxan-Induced Male White Rats

2021 ◽  
Vol 53 (3) ◽  
pp. 132-137
Author(s):  
Sri Lestari Ramadhani Nasution ◽  
◽  
Awanis ◽  
Stiven Elsafarindo
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Blood Sugar ◽  
Glucose Level ◽  
Leaf Extract ◽  
Fasting Blood Glucose ◽  
Control Group ◽  
P Value ◽  
Standard Group ◽  
White Rats

Type 2 diabetes is a metabolic disorder caused by insulin resistance and is associated with oxidative stress. In Indonesia, the mean prevalence of diabetes ranges from 1.4% to 1.6%; however, some areas have a much higher diabetes prevalence such as Pekajangan (2.3%) and in Manado (6%). The 2013 Indonesian Basic Health Research (IBHR) stated that the number of people with DM in Indonesia has reached an alarming rate. This study aimed to determine the antidiabetic effect of Polyscias scutellaria on alloxan-induced male Wistar. This was an experimental study conducted in July 2020 in the Faculty of Medicine, Prima Indonesia University. This study used 25 rats that were grouped into 5 treatment groups: control group (Na-CMC), standard (Metformin), and 3 extract groups with different doses (125 mg/kg BW, 250 mg/kg BW, and 500 mg/kg BW). Mangkokan leaf extract was obtained through the maceration method. All ratswere induced intraperitoneally using alloxan monohydrate 10% at a dose of 175 mg/kg BW. The parameters used in this study were fasting blood glucose level before induction, after induction, and after treatment and body weight before treatment. It was observed that there was a significant change in blood glucose level between the extract groups. The blood sugar level in the 125 mg/kg BW group was 495.00 mg/dl while in the 250 mg/kg BW and 500 mg/kg BW, the blood glucose levels were 317.00 mg/dl and 126.00 mg/dl, respectively, with the 500 mg/Kg BW dose as the most effective dose (P-value = 0.001). Thus, mangkokan leaves have the potential to reduce blood glucose level but are not as good as the standard group.

The Effect of Ethanol Extract of Guava Leaf on Hypercholesterolemia-Diabetes of White Rats Male Induced by High Fat Feed and Streptozotocin

2021 ◽  
Vol 7 (2) ◽  
pp. 177-187
Author(s):  
Joni Tandi ◽  
Franciska Diana Pratiwi ◽  
Surisna Surisna ◽  
Niluh Puspita Dewi ◽  
Magfirah Magfirah
Keyword(s):  
Blood Glucose ◽  
Effective Dose ◽  
Ethanol Extract ◽  
Negative Control ◽  
Control Group ◽  
Glucose Levels ◽  
White Rats ◽  
Blood Glucose Levels ◽  
Group I ◽  
Guava Leaf

This study aims to determine the effect of guava leaf extract (Psidiium guajava L) on hypercholesterolemia-diabetes and the effective dose for reducing cholesterol and blood glucose levels. This study is a laboratory experimental study using 30 rats divided into 6 test animals. the treatment group, each group consisted of 5 rats. Group I was normal control, group II negative control was given Na-CMC suspension, group III positive control was given simvastatin group IV dose 150 mg / kg BW, group V dose 250 mg / kg BW and group VI dose 350 mg / kg BW. The results showed that the ethanol extract of guava leaves had an effect on reducing blood glucose levels of male white rats with an effective dose of 250mg / kg BW with an average reduction of 119 mg/dL and effectively reduce cholesterol levels at a dose of 150 mg /kg BW with an average reduction of 28,33 mg/dL.

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