scholarly journals Penentuan Status Maya Index Larva Aedes Sp.

2020 ◽  
Vol 2 (1) ◽  
pp. 15-24
Author(s):  
Fatimah Azzahra ◽  
Emantis Rosa ◽  
Muhammad Galih Irianto ◽  
Hanna Mutiara

Demam berdarah atau DBD adalah penyakit yang ditularkan melalui vektor nyamuk Aedes aegypti dan Aedes albopictus. Maya Index dapat digunakan untukmengidentifikasi suatu area yang memiliki risiko tinggi sebagai tempat perkembangbiakan atau breeding site nyamuk Aedes sp. didasarkan pada status kebersihan lingkungan dan ketersediaan tempat–tempat yang berpotensi sebagai tempat perkembangbiakan nyamuk. Tujuan penelitian ini adalah untuk mengetahui status Maya Index, mengidentifikasi tipe tempat penampungan air atau TPA, dan mengidentifikasi jenis larva Aedes sp. yang ditemukandi kecamatan Sukabumi yang dilakukan pada bulan November-Desember 2019. Penelitian ini dilakukan dengan cara survey ke 100 rumah masyarakat di 3 kelurahan, yaitu kelurahan Nusantara, kelurahan Sukabumi dan Kelurahan Sukabumi Indah. Analisis Maya Index menggunakan Breeding Risk Index atau BRI and Hygiene Risk Indexatau HRI. Hasil dari penelitian ini menunjukkan bahwa TPA terkontrol yang paling berpotensi untuk perkembangbiakan nyamuk adalah tempat penampungan air pada dispenser, sedangan TPA tidak terkontrol yang paling berpotensi untuk perkembangbiakan nyamuk adalah kaleng bekas. Ada 2 tipe dari larva nyamuk yang ditemukan, yaitu Aedes aegypti dengan persentase 73.5% dan Aedes albopictus dengan persentase 26.5%. Status Maya Index dikategorikan kepada kategori sedang yang ditentukan bedasarkan kategoriBreeding Risk Index dan Hygiene risk index. Namun demikian, hal itu tidak berarti daerah tersebut bebas dari kasus DBD. Pengaturan dari masyarakat sangat dibutuhkan untuk mengeradikasi TPA yang memiliki potensial tinggi sebagai tempat perkembangbiakan vektor DBD.  Kata Kunci: demam berdarah, larva aedes sp., maya index  DETERMINATION THE MAYA INDEX STATUS OF AEDES SP. LARVAE                                                                                         ABSTRACT Dengue Fever is a disease that is transmitted through the Aedes aegypti and Aedes albopictus mosquito vectors. Maya Index can be used to identify an area that has a high risk of breeding site of the Aedes sp. Mosquito based on the status of environmental hygiene and the availability of potential places of mosquito breeding. The aim of this study was to determine the type of landfill which has the potential as a breeding ground for mosquitoes, to find out the larvae found in various landfill sites, and to know the Maya index status in Sukabumi District which was carried out in November-December 2019. This research conducted by a direct survey of 100 houses in 3 villages in Sukabumi District, namely Nusantara, Sekabumi, and Sukabumi Indah. Data analysis to determine Maya Index uses the Breeding Risk Index or BRI and Hygiene risk index or HRI. The results indicated that the type of controlled water reservoir that had the most potential as a breeding place for mosquitoes was a water reservoir of dispenser, while the type of water reservoir that was not controlled which had the potential as a place for mosquito breeding was a used cans. There were 2 types of larvae found, namely, Aedes aegypti larvae with a percentage of 73.5% and Aedes albopictus larvae with a percentage of 26.5%. The Maya index status detected there, was a medium category, which obtained from the Breeding Risk Index category and Hygiene Risk Index category. Besides, it did not mean that the area has been free from dengue cases. The role of the community is still very much needed in eradicating landfills which has the potential as a breeding place for dengue mosquitoes vector.  Keyword: dengue fever, larva of aedes sp., maya index

2019 ◽  
Vol 6 (1) ◽  
pp. 1-7
Author(s):  
Betara Sona ◽  
Emantis Rosa ◽  
M. Kanedi ◽  
Tugiyono Tugiyono

Dengue fever was one of the dangerous diseases due to it might cause death in a short time. It transmitted through its vectors, namely, Aedes aegypti and Aedes albopictus. Maya index is an indicator that used to identify a high-risk area or not as a breeding ground for Aedes sp. The purpose of this study was to determine the type of landfill which has the potential as a breeding ground for mosquitoes, to find out the larvae found in various landfill sites, and to know the Maya index status in Metro Timur Subdistrict which was carried out in November-December 2017. This research conducted by a direct survey of 100 residents' houses in East Metro. The results indicated that the type of controlled water reservoir that had the most potential as a breeding place for mosquitoes was a bathtub, while the type of water reservoir that was not controlled which had the potential as a place for mosquito breeding was a fish pond usage. There were 2 types of larvae found, namely, Aedes aegypti larvae with a percentage of 47.7% and Aedes albopictus larvae with a percentage of 52.3%. The Maya index status detected there, was a medium category, which obtained from the BRI combination and HRI category. Besides, it did not mean that the area has been free from dengue cases. The role of the community is still very much needed in eradicating landfills which has the potential as a breeding place for dengue mosquitoes vector.


2015 ◽  
Vol 8 (7) ◽  
pp. 543-548 ◽  
Author(s):  
Pham Thi Kim Lien ◽  
Vu Trong Duoc ◽  
Laurent Gavotte ◽  
Emmanuel Cornillot ◽  
Phan Thi Nga ◽  
...  

Biomédica ◽  
2017 ◽  
Vol 37 ◽  
pp. 135 ◽  
Author(s):  
Andrés Gómez-Palacio ◽  
Juan Suaza-Vasco ◽  
Sandra Castaño ◽  
Omar Triana ◽  
Sandra Uribe

Introducción. Aedes aegypti y Ae. albopictus son reconocidos vectores de arbovirus como los del dengue, la fiebre amarilla, el chikungunya y el Zika, en regiones tropicales y subtropicales del mundo. En Colombia, la distribución geográfica de Ae. albopictus ha sufrido un incremento y hoy incluye ciudades como Cali y Medellín. Hasta ahora, sin embargo, no se ha recabado información concluyente sobre su infección viral y su capacidad de transmisión a los humanos.Objetivo. Determinar la infección natural por dengue en ejemplares de Ae. albopictus recolectados en un área urbana de Medellín.Materiales y métodos. Se recolectaron individuos de Ae. albopictus en el campus de la Universidad Nacional de Colombia, sede Medellín. Se confirmó su clasificación taxonómica mediante el análisis del gen citocromo oxidasa I (COI), y se extrajo el ARN total para la identificación del virus del dengue y de los respectivos serotipos. La presencia del genotipo DENV se infirió mediante el análisis del gen NS3.Resultados. El análisis del COI corroboró el estatus taxonómico de Ae. albopictus. Uno de los mosquitos procesados fue positivo para DENV-2 y el análisis del NS3 mostró una gran similitud con el genotipo asiático-americano.Conclusión. Se reporta la infección con DENV-2 en Ae. albopictus en Medellín, Colombia. La presencia del genotipo asiático-americano en una zona urbana sugiere su posible circulación entre humanos y en Ae. albopictus, lo cual alerta sobre su eventual papel en la transmisión del DENV-2, y sobre la necesidad de incluir esta especie en la vigilancia entomológica en Colombia.


2015 ◽  
Vol 7 (2) ◽  
Author(s):  
Meiranty C. Pangerapan ◽  
Beivy J. Kolondam

Abstract: Dengue virus is a single-stranded RNA virus that belongs to Flaviviridae family. This virus causes dengue fever which is transmitted by Aedes aegypti dan Aedes albopictus. There are four serotypes of dengue virus; all of them can cause dengue fever. Understanding the genomics of dengue virus is important for research and diagnostics. The genome of dengue virus is 11 kilo-base long. It consists of 5’-untranslated region (5’-UTR), three structural genes (coding capsid protein, pre-membrane/membrane, and envelope), seven non-structural genes (coding NS1, NS2A, NS2B, NS3, NS4A, NS4B, and NS5 proteins) and 3’-UTR. Non-structural genes are encoding proteins of viral RNA replication, interferon response, viral assembly and secretion, endoplasmic reticulum membrane invagination induction, immune-mediator induction, and RNA 5’-caping.Keywords: dengue virus, genome, structural genes, non-structural genes, untranslated region.Abstrak: Virus dengue merupakan virus RNA beruntai tunggal yang termasuk dalam famili Flaviviridae. Virus ini adalah penyebab penyakit demam berdarah dengue yang ditransmisikan melalui nyamuk Aedes aegypti dan Aedes albopictus. Ada empat serotipe virus dengue yang telah dikenal secara luas yang ada semuanya dapat menimbulkan penyakit demam berdarah. Pemahaman tentang genomik virus dengue sangat penting untuk pengembangan penelitian dan juga untuk keperluan diagnostik. Genom virus dengue memiliki panjang 11 kilo basa. Genomnya tersusun atas 5’-untranslated region (5’-UTR), tiga gen struktural (mengodekan protein kapsid, premembran/membran dan amplop), tujuh gen non-struktural (mengodekan protein NS1, NS2A, NS2B, NS3, NS4A, NS4B dan NS5) dan 3’-UTR. Gen-gen non-struktural mengodekan protein untuk replikasi RNA virus, respon interferon, perakitan, sekresi partikel virus, menginduksi invaginasi membran retikulum endoplasma, induksi imunomediator dan penambahan tudung pada ujung 5’ RNA.Kata kunci: virus dengue, genom, gen struktural, gen non-struktural, untranslated region


2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Ivan T. Sigarlaki ◽  
Victor D. Pijoh ◽  
Josef S.B. Tuda

Abstract: Dengue hemorrhagic fever (DHF) is a viral disease that can cause death within a very short time (a few days). The main vector of DHF is Aedes aegypti mosquito, whereas the potential vector is Aedes albopictus. In the context of prevention of DHF, environmental data associated with bionomics mosquito vector of DHF, the Maya Index, are needed. DHF is still a public health problem. And for the case that there is in the city of Manado in 2013, in this case in the district of Singkil, acquired as many as 43 cases of dengue hemorrhagic fever. This study aimed to describe the Maya Index at the houses of dengue hemorrhagic fever patients in the village of West Kombos, Singkil districts. Maya Index is based on hygiene risk index (HRI) and breeding risk index (BRI). This was a descriptive study. Samples were houses of patients with DHF in 2015 from January to December. The results showed that there were four patients during the year 2015. Observation of the four houses and calculation of the data indicated that two houses were in low category of Maya Index, one house in medium category of Maya Index, and one house in high category of Maya Index. Keywords: dengue hemorrhagic fever, Maya index Abstrak: Demam berdarah dengue (DBD) adalah penyakit virus yang berbahaya karena dapat menyebabkan kematian dalam waktu yang sangat singkat (beberapa hari). Vektor utama DBD ialah nyamuk Aedes aegypti, sedangkan vektor potensialnya ialah Aedes albopictus. Dalam konteks penanggulangan DBD, juga diperlukan data lingkungan terkait dengan segi bionomik nyamuk vektor DBD, yaitu Indeks Maya. DBD masih merupakan masalah kesehatan masyarakat di kota Manado. Pada tahun 2013, di Kecamatan Singkil didapatkan sebanyak 43 kasus DBD. Penelitian ini bertujuan untuk mengetahui gambaran Indeks Maya pada rumah penderita DBD di Kelurahan Kombos Barat Kecamatan Singkil. Indeks Maya didasarkan pada hygiene risk index (HRI) dan breeding risk index (BRI). Jenis penelitian ialah deskriptif. Sampel penelitian ialah rumah dari penderita DBD tahun 2015 sejak bulan Januari-Desember. Dari hasil penelitian didapatkan empat penderita sepanjang tahun 2015 dan setelah dilakukan observasi ke rumah penderita dan perhitungan data didapatkan dua rumah masuk pada kategori Indeks Maya rendah, satu rumah masuk pada kategori Indeks Maya sedang, dan satu rumah masuk kategori Indeks Maya tinggi.Kata kunci: demam berdarah dengue, indeks Maya


2021 ◽  
Vol 12 ◽  
Author(s):  
Katherine D. Mosquera ◽  
Luis E. Martinez Villegas ◽  
Sacha J. Pidot ◽  
Chinhda Sharif ◽  
Sven Klimpel ◽  
...  

Mosquito breeding sites are complex aquatic environments with wide microbial diversity and physicochemical parameters that can change over time during the development of immature insect stages. Changes in biotic and abiotic conditions in water can alter life-history traits of adult mosquitos but this area remains understudied. Here, using microbial genomic and metabolomics analyses, we explored the metabolites associated with Aedes aegypti breeding sites as well as the potential contribution of Klebsiella sp., symbiotic bacteria highly associated with mosquitoes. We sought to address whether breeding sites have a signature metabolic profile and understand the metabolite contribution of the bacteria in the aquatic niches where Ae. aegypti larvae develop. An analysis of 32 mosquito-associated bacterial genomes, including Klebsiella, allowed us to identify gene clusters involved in primary metabolic pathways. From them, we inferred metabolites that could impact larval development (e.g., spermidine), as well as influence the quality assessment of a breeding site by a gravid female (e.g., putrescine), if produced by bacteria in the water. We also detected significant variance in metabolite presence profiles between water samples representing a decoupled oviposition event (oviposition by single females and manually deposited eggs) versus a control where no mosquito interactions occurred (PERMANOVA: p < 0.05; R2 = 24.64% and R2 = 30.07%). Five Klebsiella metabolites were exclusively linked to water samples where oviposition and development occurred. These data suggest metabolomics can be applied to identify compounds potentially used by female Ae. aegypti to evaluate the quality of a breeding site. Elucidating the physiological mechanisms by which the females could integrate these sensory cues while ovipositing constitutes a growing field of interest, which could benefit from a more depurated list of candidate molecules.


2014 ◽  
Vol 2 (3) ◽  
Author(s):  
Aprianto Jacob ◽  
Victor D. Pijoh ◽  
G. J. P. Wahongan

Abstract: In Indonesia there are two vectors are known, the main vector Aedes aegypti and Aedes albopictus as a potential vector, Aedes spp mosquito breeding varies but generally prefer clear water reservoirs. Eggs Aedes spp mosquitoes can hatch in the sewage, although not known survival and growth of larvae into pupae and adult mosquitoes. Objective: To determine the survival and growth of Aedes spp in various types of water breeding. Methods: Four types of breeding water taken directly from the settlement, and immediately used. Eggs Aedes spp laboratory strains incubated in water media. Larvae reared until the age of 4 days. A sample of 25 healthy larvae included six types of breeding water. The number of surviving larvae, pupae and adult mosquitoes be observed and counted every day for 15 days. Data security and growth of larvae processed manually in the form of percentages and graphs. Results: Aedes spp shown to survive in water dug wells (SGL), sewage water (sewer), as well as tap water. The presence of mosquitoes living in the sewer water can last up to 15 days with the same amount of mosquitoes from the first day until the last day. This phenomenon is different in the SGL and PAM water where mosquitoes can survive until day 15, although with a small percentage. Aedes spp proved unable to survive in wastewater soap. Conclusion: Water drains were left in place and clear become breeding places for Aedes spp good to note that its presence in the cleaning mosquito breeding. Keywords: The larvae of Aedes spp, life, death, pupa, adult mosquitoes, breeding water.     Abstrak: Di Indonesia dikenal ada dua vektor, vektor utama nyamuk  Aedes aegypti dan Aedes albopictus sebagaivektor potensial, perindukan nyamuk Aedes spp sangat bervariasi tetapi umumnya lebih menyukai tempat penampungan air jernih. Telur Aedes sppdapat menetas pada air comberan,meskipun belum diketahui ketahanan hidup dan pertumbuhan larva menjadi pupa dan nyamuk dewasa. Tujuan: mengetahui ketahanan hidup dan pertumbuhan nyamuk Aedes spp pada berbagai jenis air perindukan. Metode: Empat jenis air perindukan diambil secara langsung dari pemukiman penduduk dan langsung digunakan. Telur Aedes spp strain laboratorium ditetaskan pada media air bersih. Larva dipelihara hingga berumur 4 hari. Sampel sebanyak 25 ekor larva sehat dimasukkan ke enam jenis air perindukan. Jumlah larva yang bertahan hidup, menjadi pupa dan nyamuk dewasa diamati dan dihitung setiap hari selama 15 hari. Data ketahanan dan pertumbuhan larva diolah secara manual dalam bentuk persentase dan grafik. Hasil: Nyamuk Aedes spp terbukti dapat bertahan hidup pada air sumur gali (SGL), air comberan (got), serta air PAM.  Keberadaan nyamuk hidup pada air got  mampu  bertahan  sampai 15 hari dengan jumlah nyamuk yang sama dari hari pertama sampai hari terakhir. Fenomena ini berbeda pada air SGL dan PAM dimana nyamuk mampu bertahan sampai hari ke-15 meskipun dengan persentase kecil. Nyamuk Aedes spp terbukti tidak dapat bertahan hidup pada air limbah sabun. Simpulan:  Air got yang didiamkan dan jernih menjadi tempat perindukan yang baik bagi Aedes spp sehingga keberadaannya perlu diperhatikan dalam pembersihan sarang nyamuk. Kata kunci: Larva Aedes spp, hidup, mati, pupa, nyamuk dewasa, air perindukan.


Author(s):  
Yulidar Yulidar

One of vector borne deseases which still remains a health problem in Indonesia is dengue fever (DBD). This desease is caused by dengue virus transmitted by Aedes aegypti mosquitoes. One of cities which is endemic for DBD in 2014 was Banda Aceh. The indicator for DBD endemic is based on entomology index. One of entomology index to be measured is the number of mosquito larvae-free. To know the status of mosquito larvae-free number in Banda Aceh, a suvey has been conducted in three subdistricts within Banda Aceh city, namely Banda Raya (Lamlagang village), Ulee Kareng (Ceurih village), and Baiturrahman (Peuniti village). A survey was conducted in 100 houses of every subdistrict. The total of 300 houses were investigated. From 300 houses investigated, there were 158 houses were positively had mosquito larvae. From 860 containers that were found and checked in those three subdistricts, it was found that there were 235 containers were positively contained mosquito larvae. The container which contained mosquito larvae dominantly was bathups inside the houses, there were 48,22% of larvae and 60,47% of pupas. The results showed that the number of mosquito larvae-free for Banda aceh was 47,33% which indicated that it was still below the number of national larvae-free that is > 95%. Therefore, Banda Aceh is still in a high risk of DBD transmission in the future.


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