Dihydromyricetin Prevents Inflammation and Oxidative Stress in Human Osteoarthritis Chondrocytes

2021 ◽  
Vol 20 (1) ◽  
pp. 207-212
Author(s):  
Pengbo Yao ◽  
Jinwei Ai
Keyword(s):  
Oxidative Stress ◽  
Nuclear Factor Kappa B ◽  
Therapeutic Potential ◽  
Cell Model ◽  
Cartilage Degradation ◽  
Cartilage Destruction ◽  
Interleukin 1Β ◽  
Nuclear Factor ◽  
Nuclear Factor Kappa

Osteoarthritis is characterized by inflammation and joint cartilage degradation. Dihydromyricetin, a natural flavonoid in rattan tea, exhibits several pharmacological properties including antitumor, cardioprotection, antidiabetes, neuroprotection, hepatoprotection, and dermatoprotection. Herein, we have investigated in vitro the effects of dihydromyricetin on osteoarthritis progression using an interleukin-1β-induced cell model. Our data show that dihydromyricetin markedly improved the viability of interleukin-1β-treated chondrocytes. Furthermore, dihydromyricetin markedly downregulated the expression of nitric oxide, prostaglandin-E2, tumor necrosis factor-α, and interleukin-6, and ameliorated cartilage destruction in interleukin- 1β-treated chondrocytes, as well as inhibited the interleukin-1β-induced oxidative stress via the nuclear factor kappa B axis. In summary, our results demonstrate therapeutic potential of dihydromyricetin in osteoarthritis through modulation of the nuclear factor kappa B signaling pathway.

L-Tetrahydropalmatine Inhibits the Progression of Glioblastoma Tumor by Suppressing the Extracellular-Signal-Regulated Kinase/Nuclear Factor-Kappa B Signaling Pathway

2020 ◽  
Vol 19 (2) ◽  
pp. 164-171
Author(s):  
Feng Xue ◽  
Tingting Chen
Keyword(s):  
Glioblastoma Multiforme ◽  
Nuclear Factor Kappa B ◽  
Matrix Metalloproteinase 2 ◽  
Nuclear Factor ◽  
Nuclear Factor Kappa ◽  
Extracellular Signal ◽  
Apoptosis Protein ◽  
Endothelial Growth Factor

Glioblastoma multiforme is the most common malignancy of central nervous system. Herein we have evaluated the effect of L-tetrahydropalmatine, an isoquinoline alkaloid, on the tumor growth both in vivo and in vitro using C6 glioblastoma multiforme cells and BALB/c mice injected subcutaneously with C6/luc2 cells. The results of these studies show that L-tetrahydropalmatine exhibited cytotoxic effect on C6 glioblastoma multiforme cells, suppressed nuclear factor-kappa B activity, suppressed the levels of tumor-linked proteins such as matrix metalloproteinase-2/9, Cyclin-D1, vascular endothelial growth factor, and X-linked inhibitor of apoptosis protein via ERK/nuclear factor-kappa B cascade. Further, L-tetrahydropalmatine inhibited the cell migration and invasion properties of C6 cells, and also suppressed the tumor weight and volume in mice. Immunohistochemical staining of tumor tissues suggested that L-tetrahydropalmatine inhibited the extracellular-signal-regulated kinase/nuclear factor-kappa B cascade and suppressed the levels of Cyclin-D1; matrix metalloproteinase-2/9; X-linked inhibitor of apoptosis protein; and vascular endothelial growth factor, and also the progression and growth of glioblastoma multiforme in mice. In summary, L-tetrahydropalmatine inhibits the ERK/nuclear factor-kappa B cascade, decreases the tumor volume, and inhibits the proteins responsible for tumor growth both in vivo and in vitro.

scholarly journals Brain angiotensin or nuclear factor kappa B blockade attenuates cytokines, oxidative stress and decreases sympathoexcitation in hypertension

2008 ◽  
Vol 22 (S1) ◽  
Author(s):  
Yu‐Ming Kang ◽  
Ying Ma ◽  
Srinivas Sriramula ◽  
Jin‐Ping Zheng ◽  
Zhi‐Ming Yang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Nuclear Factor Kappa B ◽  
Nuclear Factor ◽  
Nuclear Factor Kappa
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