Isolation and characterization of 50 nuclear microsatellite markers for Cathaya argyrophylla , a Chinese endemic conifer

2010 ◽  
Vol 97 (11) ◽  
pp. e117-e120 ◽  
Author(s):  
Zhao-Shan Wang ◽  
Hai-Qin Sun ◽  
Hong-Wei Wang ◽  
Song Ge
Keyword(s):  
Microsatellite Markers ◽  
Isolation And Characterization ◽  
Nuclear Microsatellite

Cross-species amplification and characterization of microsatellite loci in Pinus mugo Turra

Biologia ◽  
2013 ◽  
Vol 68 (4) ◽  
Author(s):  
Konrad Celiński ◽  
Ewa Pawlaczyk ◽  
Aleksandra Wojnicka-Półtorak ◽  
Ewa Chudzińska ◽  
Wiesław Prus-Głowacki
Keyword(s):  
Microsatellite Markers ◽  
Polymorphic Information Content ◽  
Pinus Mugo ◽  
Genetic Studies ◽  
Transfer Rates ◽  
Isolation And Characterization ◽  
Nuclear Microsatellite ◽  
European Mountain ◽  
High Level

AbstractPinus mugo (dwarf mountain pine) is an important component of European mountain ecosystems. However, little is known about the present genetic structure and population differentiation of this species at the DNA level, possibly due to a lack of nuclear microsatellite markers (SSR) developed for Pinus mugo. Therefore in this study we transferred microsatellite markers originally developed for Pinus sylvestris and Pinus taeda to Pinus mugo. This cross-species amplification approach is much faster and less expensive than isolation and characterization of new microsatellite markers. The transfer rates from the source species to Pinus mugo were moderately low (26%). There were no differences in microsatellite repeat motifs between the source species and Pinus mugo. Nuclear microsatellite markers successfully transferred to Pinus mugo can be applied to various genetic studies on this species, due to the high level of their polymorphism and high value of polymorphic information content.

scholarly journals Short Note: Isolation and Characterization of 12 Polymorphic Microsatellite Markers in Engelhardia roxburghiana (Juglandaceae)

2014 ◽  
Vol 63 (1-6) ◽  
pp. 109-112 ◽  
Author(s):  
Dan-Dan Zhang ◽  
Pi Luo ◽  
Ying Chen ◽  
Zheng-Feng Wang ◽  
Wan-Hui Ye ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Short Note ◽  
Screening Method ◽  
Wide Distribution ◽  
Enriched Library ◽  
Isolation And Characterization ◽  
Nuclear Microsatellite ◽  
Polymorphic Microsatellite

Abstract Engelhardia roxburghiana is a common half evergreen tree with a wide distribution in southeast Asia. Despite its ecological and pharmaceutical values, its genetic diversity is poorly studied. Our objective was to develop nuclear microsatellite markers to investigate the level of genetic diversity within and among populations in the future. Using the microsatellite-enriched library and PCR-based screening method, 12 microsatellite markers were developed and showed polymorphism in a population. The number of alleles per locus for these 12 microsatellites ranged from four to 15. The observed and expected heterozygosities ranged from 0.358 to 0.897 and from 0.369 to 0.886, respectively. The developed microsatellites will be useful for studying genetic diversity and population structure in E. roxburghiana.

Isolation and characterization of novel polymorphic nuclear microsatellite markers from Ophrys fusca (Orchidaceae) and cross-species amplification

2008 ◽  
Vol 10 (3) ◽  
pp. 739-742 ◽  
Author(s):  
Helena C. Cotrim ◽  
Filipa A. Monteiro ◽  
Eva S. Sousa ◽  
Michael F. Fay ◽  
Mark W. Chase ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Isolation And Characterization ◽  
Nuclear Microsatellite

scholarly journals Isolation and Characterization of Microsatellite Markers for Shorea platyclados (Dipterocarpaceae)

2013 ◽  
Vol 1 (7) ◽  
pp. 1200538 ◽  
Author(s):  
Chin Hong Ng ◽  
Chai Ting Lee ◽  
Soon Leong Lee ◽  
Lee Hong Tnah ◽  
Kevin Kit Siong Ng
Keyword(s):  
Microsatellite Markers ◽  
Isolation And Characterization ◽  
Shorea Platyclados
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