scholarly journals Uji Bioaktivitas Ekstrak Buah Sawo Manila (Manilkara Zapota) Terhadap Pertumbuhan Bakteri Salmonella Typhi

2019 ◽  
Vol 4 (1) ◽  
pp. 11-16
Author(s):  
Subakhir Salnus ◽  
Nurhamsiah
Keyword(s):  
Antimicrobial Activity ◽  
Salmonella Typhi ◽  
Test Method ◽  
Laboratory Research ◽  
Growth Media ◽  
Diffusion Test ◽  
Gram Negative ◽  
Manilkara Zapota ◽  
Know How ◽  
Growth Of Bacteria

This research was conducted bioactivities by using raw fruits of sapodilla sapota (Manilkara zapota) as a drag growth media of Salmonella typhi bacteria causes typhoid fever disease. Salmonella typhi is a species of bacteria that are gram-negative bacilli shaped, facultative, aerobic, moving with a single pertrich. Sapdilla sapota has antimicrobial activity against Salmonella typhi, because it contains a variety of secondary metabolite compounds such as saponins, tannins and flavonoids.  The purpose of this research was to know how efective the raw fruits of sapodilla sapota (Manilkara zapota) against the growth of Salmonella typhi. This is an laboratory research by using diffusion test method. The sample of this research is to extract the fruit of sapodilla sapota (Manilkara zapota) crude. The results of this research, are 35% concentration of extract cannot be formed drag zone (0 mm), but in 40, 45, 50 and 55% of concentration there are had drag zone (9 mm, 13 mm, 13.5 mm, and 18 mm). this can be said that the raw fruit of sapodilla sapota extract can inhibit the growth of Salmonella typhi bacteria can be done, then the next extension to the sufferers of the disease typhus to consume raw fruits of sapodilla sapota so that can reduce the growth of bacteria.

scholarly journals UJI BIOAKTIVITAS EKSTRAK BUAH SAWO MANILA (Manilkara zapota) TERHADAP PERTUMBUHAN BAKTERI Salmonella typhi

2019 ◽  
Vol 4 (1) ◽  
pp. 11-16
Author(s):  
Subakhir Salnus ◽  
Nurhamsia
Keyword(s):  
Antimicrobial Activity ◽  
Salmonella Typhi ◽  
Test Method ◽  
Laboratory Research ◽  
Growth Media ◽  
Diffusion Test ◽  
Gram Negative ◽  
Manilkara Zapota ◽  
Know How ◽  
Growth Of Bacteria

This research was conducted bioactivities by using raw fruits of sapodilla sapota (Manilkara zapota) as a drag growth media of Salmonella typhi bacteria causes typhoid fever disease. Salmonella typhi is a species of bacteria that are gram-negative bacilli shaped, facultative, aerobic, moving with a single pertrich. Sapdilla sapota has antimicrobial activity against Salmonella typhi, because it contains a variety of secondary metabolite compounds such as saponins, tannins and flavonoids.  The purpose of this research was to know how efective the raw fruits of sapodilla sapota (Manilkara zapota) against the growth of Salmonella typhi. This is an laboratory research by using diffusion test method. The sample of this research is to extract the fruit of sapodilla sapota (Manilkara zapota) crude. The results of this research, are 35% concentration of extract cannot be formed drag zone (0 mm), but in 40, 45, 50 and 55% of concentration there are had drag zone (9 mm, 13 mm, 13.5 mm, and 18 mm). this can be said that the raw fruit of sapodilla sapota extract can inhibit the growth of Salmonella typhi bacteria can be done, then the next extension to the sufferers of the disease typhus to consume raw fruits of sapodilla sapota so that can reduce the growth of bacteria.

scholarly journals Uji Bioaktivitas Ekstrak Buah Sawo Manila (Manilkara Zapota) Terhadap Pertumbuhan Bakteri Salmonella Typhi

2019 ◽  
Vol 6 (1) ◽  
pp. 32
Author(s):  
Subakir Salnus ◽  
Artati Artati
Keyword(s):  
Antimicrobial Activity ◽  
Typhoid Fever ◽  
Salmonella Typhi ◽  
Test Method ◽  
Diffusion Test ◽  
Manilkara Zapota ◽  
Growth Of Bacteria

This research tested the bioactive by using rawfruits of Sapodilla Sapota (Manilkara zapota) as a drag growthmedia of Salmonella Typhi bacteria causes typhoid fever disease.Salmonella typhi is a species of bacteria that are gram-negativebacilli shaped, facultative, aerobic, and moving with a singlePertrich. Sapdilla Sapota has antimicrobial activity againstSalmonella typhi, because it contains a variety of secondarymetabolite compounds such as Saponins, Tannins andFlavonoids. The purpose of this research was to know howefective the raw fruits of sapodilla sapota (Manilkara Zapota)against the growth of Salmonella typhi. This is a laboratoryyresearch by using diffusion test method. The sample of thisresearch is to extract the fruit of Sapodilla Sapota (ManilkaraZapota) crude. The results of this research are 35%concentration of extract cannot be formed drag zone (0 mm), butin 40, 45, 50 and 55% of concentration there are had drag zone (9mm, 13 mm, 13.5 mm, and 18 mm). This can be said that the rawfruit of Sapodilla Sapota extract can inhibit the growth ofSalmonella Typhi bacteria can be done, then the next extension tothe sufferers of the disease typhus to consume raw fruits ofSapodilla Sapota so that can reduce the growth of bacteria.

Synergistic Effects of Essential Oils of Ammoides verticillata and Satureja candidissima Against Many Pathogenic Microorganisms

2020 ◽  
Vol 18 (1) ◽  
pp. 72-78 ◽  
Author(s):  
Fatima Benyoucef ◽  
Mohammed El Amine Dib ◽  
Boufeldja Tabti ◽  
Arrar Zoheir ◽  
Jean Costa ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Synergistic Effects ◽  
Lactobacillus Rhamnosus ◽  
Salmonella Typhi ◽  
Antimicrobial Activities ◽  
Bacterial Strains ◽  
Gram Negative ◽  
Ammoides Verticillata

Background: Antibiotic resistance is today one of the most serious threats to global health, food security and development. Due to the growing number of infections, treatment becomes more difficult, if not impossible, because of the loss of antibiotic efficacy. Objective: In the present investigation, the chemical composition of essential oils of Ammoides verticillata and Satureja candidissima and their synergistic effects on antimicrobial activities were investigated. Methods: The chemical composition of the essential oil was analyzed by Gas Chromatography (GC) and Gas Chromatography-Mass Spectroscopy (GC/MS). The antimicrobial activity of the essential oils was investigated using dilution-agar method against nine bacterial strains three Gram-negative Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853) and Salmonella typhi (ATCC 14028), and six Gram-positive: Staphylococcus aureus (ATCC 43300,) Clostridium sporogenes (ATCC 19404), Bacillus subtilis (ATCC 6633), Enterococcus faecalis (ATCC 7314), Lactobacillus rhamnosus (ATCC 53103) and Bacillus cereus (ATCC 14579). Results: The essential oil of A. verticillata was characterized principally by carvacrol (44,3%), Limonene (19,3%) and p-cymene (19,2%). The constituents identified of S. candidissima essential oil were principally oxygenated monoterpenes represented by pulegone (70,4%). The essential oil of A. verticillata had a good antimicrobial activity against four bacterial strains (Escherichia coli, Salmonella typhi, Lactobacillus rhamnosus and Bacillus cereus) with MIC and MBC values between 0.2-0.4 µl/ml and 0.2-6.2 µl/ml, respectively. While, S. candidissima essential oil had moderate antimicrobial activities against all strains with MIC and MBC values between 1.5-6.2 µl/ml and 6.2-12.5 µl/ml, respectively. The results of antimicrobial activity of essential oils blend presented higher antimicrobial activity against all tested bacteria with MIC and MBC values between 0.3-1.5 µl/ml and 0.4-6.2 µl/ml, respectively. Conclusion: The essential oils blend presented high antimicrobial activity compared to virgin oils. This activity can be due to the association of active compounds such as carvacrol and pulegone. These findings provide a new source of drugs that may help in therapy to lead to the development of a new treatment based on a combination of these essential oils against gram-negative and gram-positive bacteria that continue to pose a threat to public health.

scholarly journals Uji Efektivitas Ekstrak Kayu Tumbuhan Biau (Psophocarpus sp.) Terhadap Bakteri Salmonella typhi Dan Shigella dysenteriae Penyebab Diare

2019 ◽  
Vol 5 (1) ◽  
pp. 28-38
Author(s):  
Oecy Mardianti ◽  
Welly Darwis ◽  
Mardhatillah Sariyanti
Keyword(s):  
Minimal Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Salmonella Typhi ◽  
Scientific Data ◽  
Effective Concentration ◽  
Test Method ◽  
Shigella Dysenteriae ◽  
Disk Diffusion ◽  
Diffusion Test ◽  
Disk Diffusion Test

ABSTRAKLatar Belakang: Penelitian ini merupakan suatu studi ethnomedicine yang bertujuan untuk mendapatkan data ilmiah mengenai efek penggunaan kayu tumbuhan Biau sebagai salah satu obat diare yang banyak digunakan oleh masyarakat Suku Serawai di Kecamatan Pino Raya. Adapun yang menjadi latar belakang penulisan ini karena Data Dinas Kesehatan Provinsi Bengkulu menunjukkan kasus diare mengalami peningkatan, yaitu sebanyak 50.622 kasus pada tahun 2016 dan 53.348 kasus pada tahun 2017. Selain itu, pemanfaatan tumbuhan obat juga dapat menjadi alternatif dalam mengatasi berbagai efek samping penggunaan antibiotik dan fenomena resistensi antibiotik yang semakin meluas.Metode: Jenis penelitian ini adalah studi analitik eksperimental laboratorium dan menggunakan metode uji difusi cakram (disk diffusion test). Ekstraksi kayu tumbuhan Biau dilakukan dengan metode maserasi menggunakan pelarut etanol 96% selama 5 hari. Hasil ekstraksi kemudian dilakukan uji Minimal Inhibitory Concentration (MIC), dan dilanjutkan uji efektivitas. Parameter yang digunakan adalah diameter zona hambat yang terbentuk disekitar kertas cakram, dan kontrol positif yang digunakan adalah larutan antibiotik siprofloksasin.Hasil: Hasil uji MIC menunjukkan bahwa kayu tumbuhan Biau mempunyai aktivitas sebagai antibakteri yang dilanjutkan dengan pengujian efektivitas. Berdasarkan analisis statistik, konsentrasi 55% merupakan konsentrasi paling efektif untuk bakteri Salmonella typhi dengan diameter 6,20 mm termasuk dalam kategori sedang, dan konsentrasi paling efektif untuk bakteri Shigella dysenteriae adalah konsentrasi 70% dengan diameter 7,66 mm termasuk dalam kategori sedang yang ditandai dengan hasil statistik yang berbeda nyata.Kesimpulan: Ekstrak kayu tumbuhan Biau mempunyai aktivitas sebagai antibakteri dengan konsentrasi 55% sebagai konsentrasi paling efektif untuk bakteri Salmonella typhi dan konsentrasi 70% merupakan konsentrasi paling efektif untuk bakteri Shigella dysenteriae. Kata kunci: Kayu Biau (Psophocarpus sp.), Shigella dysenteriae dan Salmonella typhi. ABSTRACTBackgound: This research is a study of ethnomedicine that aims to obtain scientific data about the effects of the use of Biau wood as one remedy of diarrhea that is widely used by the people of the Serawai Tribe in Pino Raya sub-district. The background of this research is Data from public health office in Bengkulu Province showed that cases of diarrhea have increased from 50,622 cases in 2016 and became 53,348 cases in 2017. In addition, the utilization of medicinal plants can also be an alternative to resolve the various side effects of antibiotic use and antibiotic resistance phenomenon which is increasingly widespread.Methods: This type of research is a laboratory experimental analytical study and used a disk diffusion test method. Extraction of Biau plant wood was done by maceration method using 96% ethanol for 5 days. The extraction results then used to Minimal Inhibitory Concentration (MIC) assay, then effectiveness assay in which both of these assay looked the diameter of inhibition zone formed around the disc paper, and the positive control used ciprofloxacin antibiotic as comparison.Results: MIC test results showed that Biau plant wood had an antibacterial activity which followed by effectiveness assay. Based on the statistical analysis showed that 55% of concentration was the most effective for Salmonella typhi with a diameter of 6.20 mm included in the moderate category and 70% of concentration was the most effective for Shigella dysenteriae with a diameter of 7.66 mm included in the moderate category which was marked by statistically different results.Conclusion: Biau wood extract had an activity as an antibacterial with a concentration of 55% as the most effective concentration for Salmonella typhi and a concentration of 70% as the most effective concentration for Shigella dysenteriae. Keywords: Biau wood (Psophocarpus sp.), Shigella dysenteriae and Salmonella typhi.

Synthesis, Characterization and Antimicrobial Activity of Bis-Acetylferrocene Schiff Base

2011 ◽  
Vol 396-398 ◽  
pp. 1875-1878 ◽  
Author(s):  
Yu Ting Liu ◽  
Hai Long Guo ◽  
Da Wei Yin
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Salmonella Typhi ◽  
Bacterial Strains ◽  
Gram Negative ◽  
And Staphylococcus Aureus

Three bis-acetylferrocene schiff bases have been synthesized and characterized by IR, 1H NMR, and elemental analysis, the results conformed well with expected structures. The synthesized compounds were screened in vitro for their antimicrobial activity against three Gram-negative (Escherichia coli, Pseudomonas aeruginosa, and Salmonella typhi) and two Gram-positive (Bacillus subtilis and Staphylococcus aureus) bacterial strains. The results showed that these compounds are show excellent antimicrobia activities against Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi ,Bacillus subtilis,Staphylococcus aureus.

scholarly journals UJI ANTIMIKROBA EKSTRAK DAN FRAKSI TUNIKATA Didemnum molle TERHADAP PERTUMBUHAN Escherichia coli, Staphylococcus aureus, DAN Candida albicans YANG DIKOLEKSI DI SELAT LEMBEH BITUNG

PHARMACON ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 275
Author(s):  
Novia A. Pitoy ◽  
Adithya Yudistira ◽  
Defny S. Wewengkang
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Microbial Growth ◽  
Test Method ◽  
Chloroform Fraction ◽  
Marine Biota ◽  
Disc Diffusion ◽  
Diffusion Test

ABSTRACTTunicate Didemnum molle is a marine biota that has bioactive components that can be used as raw material for medicines. This study aims to determine the antimicrobial activity of extracts and fractions of Didemnum molle on microbial growth of Escherichia coli, Staphylococcus aureus and Candida albicans. The sample was extracted by maceration using ethanol and fractionated using, n-hexane, chloroform and methanol. Antimicrobial activity was tested using the disc diffusion test method (Kirby and Bauer). Ethanol extract of Didemnum molle inhibited the growth of microbes of Staphylococcus aureus (8.12 mm) and Candida albicans (9.00 mm). Chloroform fraction inhibited the growth of Staphylococcus aureus (9.10 mm), Escherichia coli (10.00 mm) and Candida albicans (9.65 mm). While the methanol fraction is only able to inhibit microbial growth of Candida albicans (10.00 mm). The hexane fraction shows no activity against all test microbes. Keywords : Tunikata Didemnum molle, antimicrobial, Escherichia coli, Staphylococcus   aureus, Candida albicans.                                                                                                     ABSTRAKTunikata Didemnum molle merupakan biota laut yang memiliki komponen bioaktif yang dapat dimanfaatkan sebagai bahan baku obat-obatan. Penelitian ini bertujuan untuk mengetahui aktivitas antimikroba dari ekstrak dan fraksi tunikata Didemnum molle terhadap pertumbuhan mikroba Escherichia coli, Staphylococcus aureus dan Candida  albicans. Sampel diekstraksi dengan cara maserasi menggunakan etanol dan difraksinasi menggunakan, n-heksan, kloroform dan metanol. Aktivitas antimikroba diuji menggunakan metode disc diffusion test (Kirby dan Bauer). Ekstrak etanol Didemnum molle menghambat pertumbuhan mikroba Staphylococcus aureus (8,12 mm) dan Candida albicans (9,00 mm). Fraksi kloroform menghambat pertumbuhan mikroba Staphylococcus aureus (9,10 mm), Escherichia coli (10,00 mm) dan Candida albicans (9,65 mm). Sementara fraksi metanol hanya mampu menghambat pertumbuhan mikroba Candida albicans (10.00 mm). Fraksi heksan tidak menunjukkan aktivitas terhadap semua mikroba uji. Kata Kunci : Tunikata Didemnum molle, antimikroba, Escherichia coli, Staphylococcus aureus, Candida albicans.

scholarly journals Methylglyoxal: Antimicrobial activity against blood culture isolates of Salmonella Typhi and other Gram negative rods

2019 ◽  
Vol 35 (4) ◽  
Author(s):  
Raja Kamran Afzal ◽  
Fizza Khalid ◽  
Abdul Hannan ◽  
Syed Azhar Ahmed
Keyword(s):  
Antimicrobial Activity ◽  
Blood Culture ◽  
Salmonella Typhi ◽  
P Value ◽  
Gram Negative ◽  
Creative Commons ◽  
Serological Identification ◽  
Agar Dilution

Objectives: To evaluate the activity of Methylglyoxal against the blood culture isolates of Salmonella Typhi and various Gram negative rods and to compare the activity of Methylglyoxal against S. Typhi and other Gram negative rods. Methods: It was an experimental study conducted at the Department of Microbiology, University of Health Sciences (UHS), Lahore-Pakistan in collaboration with the Department of Microbiology, CMH Lahore, from July 2011 to June 2012. Recent blood culture isolates of S. Typhi and other Gram negative rods were collected from different hospitals of Lahore and kept stored at -80°C. As per the latest CLSI guidelines, morphological, biochemical and serological identification was carried out and antimicrobial susceptibility was tested. A multi-point inoculator was used to carry out agar dilution for determination of MICs of MGO. Results were determined after compilation of data using latest SPSS version. Results: MIC90 of MGO against the clinical isolates of S. Typhi was 0.20 mg/mL (2.8 mM) and against Gram negative rods it was 0.21 mg/mL (3.0 mM). The p-value of MICs of MGO against the isolates of S. Typhi was 0.023 when compared with Gram negative rods (p<0.05; statistically significant). Conclusion: MGO has a scientifically proven in vitro antimicrobial activity against blood culture isolates of S. Typhi and various Gram negative rods. doi: https://doi.org/10.12669/pjms.35.4.807 How to cite this:Afzal RK, Khalid F, Hannan A, Ahmed SA. Methylglyoxal: Antimicrobial activity against blood culture isolates of Salmonella Typhi and other Gram negative rods. Pak J Med Sci. 2019;35(4):---------. doi: https://doi.org/10.12669/pjms.35.4.807 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

scholarly journals Antimicrobial Activity of Coleus Ambonicus Herbal Finish on Cotton Fabric

2017 ◽  
Vol 25 (0) ◽  
pp. 106-109 ◽  
Author(s):  
M. Gopalakrishnan ◽  
D. Saravanan
Keyword(s):  
Antimicrobial Activity ◽  
Cotton Fabric ◽  
Test Method ◽  
Bacterial Reduction ◽  
Gram Positive ◽  
Gram Negative ◽  
Low Levels ◽  
Wash Durability ◽  
Encapsulation Methods ◽  
Reduction Percentage

An extract obtained from Coleus Ambonicus was applied on cotton fabric by means of the exhaust, micro encapsulation and nano encapsulation methods, and the antimicrobial activity of the finished fabric assessed quantitatively by the AATCC test method 100 against gram positive (staphylococcus aureus) and gram negative (Escherichia coli) microbes. The finish applied on the the samples using all three methods exhibit a good bacterial reduction percentage. The finish applied on the samples using all three methods possesses a higher bacterial reduction percentage against gram positive microbes than gram negative, even after washing. The method of washing conforms to ISO method 3. The wash durability of the antimicrobial activity was assessed by the bacterial reduction percentage after washing. The wash durability of the samples using the direct exhaust method was very poor and it lost its antimicrobial activity after 10 wash cycles. The wash durability of the samples using the micro encapsulated method shows antimicrobial activity up to 10 wash cycles, dropping gradually to very low levels at 20 wash cycles. The wash durability of the samples using nano encapsulation shows good antimicrobial activity against both gram positive and gram negative microbes even after 30 washes

SYNTHESIS, CHARACTERIZATION AND BIOLOGICAL EVALUATION OF 3-FORMYL INDOLE BASED SCHIFF BASES

2018 ◽  
Vol 7 (6) ◽  
Author(s):  
Singh Gurvinder ◽  
Singh Prabhsimran ◽  
Dhawan R. K.
Keyword(s):  
Antimicrobial Activity ◽  
Spectral Analysis ◽  
Schiff Bases ◽  
Antimicrobial Agents ◽  
Biological Evaluation ◽  
Fungal Strain ◽  
Bacterial Strains ◽  
Standard Drug ◽  
Gram Negative ◽  
E Coli

In order to develop new antimicrobial agents, a series of 3-formyl indole based Schiff bases were synthesized by reacting 3-formyl indole(indole-3-carboxaldehyde) with substituted aniline taking ethanol as solvent. The reaction was carried in the presence of small amount of p-toluene sulphonic acid as catalyst.All the synthesized compounds were characterized by IR, 1H-NMR spectral analysis. All the synthesized compounds were evaluated for antimicrobial activity against two gram positive bacterial strains (B. subtilisand S. aureus) and two gram negative bacterial strains (P. aeruginosaand E. coli) and one fungal strain (C. albicans). All the synthesized compounds were found to have moderate to good antimicrobial activity. The  standard drug amoxicillin, fluconazole were used for antimicrobial activity. Among the synthesized compounds, the maximum antimicrobial activity was shown by compounds GS04, GS07, GS08 and GS10.

Disinfection of Fruits with Activated Water

2019 ◽  
Vol 10 ◽  
pp. 1864-1872
Author(s):  
Prof. Teodora P. Popova
Keyword(s):  
Antimicrobial Activity ◽  
Aqueous Solutions ◽  
Antimicrobial Properties ◽  
The Other ◽  
Gram Negative Bacteria ◽  
High Antimicrobial Activity ◽  
Gram Negative ◽  
E Coli ◽  
Number Of Microorganisms ◽  
Lower Effect

The effect of ionized aqueous solutions (anolytes and catholyte) in the processing of fruits (cherries, morellos, and strawberries) for decontamination has been tested. Freshly prepared analytes and catholyte without the addition of salts were used, as well as stored for 7 months anolytes, prepared with 0.5% NaCl and a combination of 0.5% NaCl and 0.5% Na2CO3. The anolyte prepared with a combination of 0.5% NaCl and 0.5% Na2CO3, as well as the anolyte obtained with 0.5% NaCl, exhibit high antimicrobial activity against the surface microflora of strawberries, cherries, and sour cherries. They inactivate E. coli for 15 minutes. The other species of the fam. Enterobacteriaceae were also affected to the maximum extent, as is the total number of microorganisms, especially in cherries and sour cherries. Even stored for 7 months, they largely retain their antimicrobial properties. Anolyte and catholyte, obtained without the addition of salts, showed a lower effect on the total number of microorganisms, but had a significant effect on Gram-negative bacteria, and especially with regard to the sanitary indicative E. coli.

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