Inhibitory Effect of Lithospermum erythrorhizon Extracts on Melanin Biosynthesis

2005 ◽  
Vol 34 (9) ◽  
pp. 1325-1329 ◽  
Keyword(s):  
Inhibitory Effect ◽  
Lithospermum Erythrorhizon ◽  
Melanin Biosynthesis

scholarly journals Inhibitory Effect of Gnetin C, a Resveratrol Dimer from Melinjo (Gnetum gnemon), on Tyrosinase Activity and Melanin Biosynthesis

2012 ◽  
Vol 35 (6) ◽  
pp. 993-996 ◽  
Author(s):  
Miyako Yanagihara ◽  
Maiko Yoshimatsu ◽  
Akinori Inoue ◽  
Tomoko Kanno ◽  
Tomoki Tatefuji ◽  
...  
Keyword(s):  
Inhibitory Effect ◽  
Tyrosinase Activity ◽  
Melanin Biosynthesis ◽  
Gnetum Gnemon

Molecular characterization of prophenoloxidase-1 (PPO1) and the inhibitory effect of kojic acid on phenoloxidase (PO) activity and on the development ofZeugodacus tau(Walker) (Diptera: Tephritidae)

2018 ◽  
Vol 109 (2) ◽  
pp. 236-247 ◽  
Author(s):  
H.-H. Zhang ◽  
M.-J. Luo ◽  
Q.-W. Zhang ◽  
P.-M. Cai ◽  
A. Idrees ◽  
...  
Keyword(s):  
Kojic Acid ◽  
Normal Growth ◽  
Competitive Inhibitor ◽  
Inhibitory Effect ◽  
Pupal Weight ◽  
Mrna Levels ◽  
Melanin Biosynthesis ◽  
Horticultural Pest ◽  
Inhibition Experiment

AbstractPhenoloxidase (PO) plays a key role in melanin biosynthesis during insect development. Here, we isolated the 2310-bp full-length cDNA of PPO1 fromZeugodacus tau, a destructive horticultural pest. qRT-polymerase chain reaction showed that theZtPPO1transcripts were highly expressed during larval–prepupal transition and in the haemolymph. When the larvae were fed a 1.66% kojic acid (KA)-containing diet, the levels of theZtPPO1transcripts significantly increased by 2.79- and 3.39-fold in the whole larvae and cuticles, respectively, while the corresponding PO activity was significantly reduced; in addition, the larval and pupal durations were significantly prolonged; pupal weights were lowered; and abnormal phenotypes were observed. Anin vitroinhibition experiment indicated that KA was an effective competitive inhibitor of PO inZ. tau. Additionally, the functional analysis showed that 20E could significantly up-regulate the expression ofZtPPO1, induce lower pupal weight, and advance pupation. Knockdown of theZtPPO1gene by RNAi significantly decreased mRNA levels after 24 h and led to low pupation rates and incomplete pupae with abnormal phenotypes during the larval-pupal interim period. These results proved that PO is important for the normal growth ofZ. tauand that KA can disrupt the development of this pest insect.

scholarly journals Inhibitory effect of aflastatin A on melanin biosynthesis by Colletotrichum lagenarium

Microbiology ◽  
2001 ◽  
Vol 147 (9) ◽  
pp. 2623-2628 ◽  
Author(s):  
Susumu Okamoto ◽  
Masaru Sakurada ◽  
Yasuyuki Kubo ◽  
Gento Tsuji ◽  
Isao Fujii ◽  
...  
Keyword(s):  
Inhibitory Effect ◽  
Colletotrichum Lagenarium ◽  
Melanin Biosynthesis

Inhibition of Tyrosinase by Protocatechuic Aldehyde

2004 ◽  
Vol 32 (01) ◽  
pp. 97-103 ◽  
Author(s):  
Jae Kyung No ◽  
Min Sun Kim ◽  
You Jung Kim ◽  
Song Ja Bae ◽  
Jae Sue Choi ◽  
...  
Keyword(s):  
Competitive Inhibition ◽  
Salvia Miltiorrhiza ◽  
Inhibitory Effect ◽  
Tyrosinase Activity ◽  
Medical Problems ◽  
Melanin Biosynthesis ◽  
Rate Limiting Step ◽  
Protocatechuic Aldehyde ◽  
Dependent Inhibition ◽  
Potent Inhibitory Effect

The purpose of this study was to determine the inhibitory action of protocatechuic aldehyde (PCA) on tyrosinase activity. PCA is one of the compounds found in the root of Salvia miltiorrhiza. Our study documented that PCA has a potent inhibitory effect on tyrosinase, which catalyzes the rate-limiting step of melanin biosynthesis. Although melanin biosynthesis has an essential function normally in human skin for defense against ultraviolet light of the sun, its abnormal activity as seen in pigmentation disorder could lead to serious medical problems. Our data showed that PCA, with concentrations ranging from 1×10-5 M to 8×10-5 M , exhibited dose-dependent inhibition of the enzyme activity with 50% of inhibition at 19.92×10-6 M . A further kinetic analysis on PCA inactivation of tyrosinase activity revealed a competitive inhibition of the enzyme at the L-tyrosine binding site. The findings of our present study merit further research on the applicability of PCA as a potential agent for treatment of pigmentation disorder.

Anti-Melanogenic Activity of p-Chlorophenyl Benzyl Ether in α-MSH-Induced Mouse Melanoma B16F10 Cells

2019 ◽  
Vol 819 ◽  
pp. 118-123
Author(s):  
Wassana Riam-Amatakun ◽  
Panupan Limpachayaporn ◽  
Jhoan Rhea L. Pizon ◽  
Praneet Opanasopit ◽  
Nopparat Nuntharatanapon
Keyword(s):  
Kojic Acid ◽  
Positive Association ◽  
Inhibitory Effect ◽  
Tyrosinase Activity ◽  
Dependent Manner ◽  
Benzyl Ether ◽  
Melanin Content ◽  
Synthetic Compound ◽  
Melanin Biosynthesis ◽  
B16f10 Cells

Melanin is cutaneous pigment which level of its production determines skin complexion. Overproduction of melanin, frequently promoted by UV rays, results in darkening of the skin. Inhibition of tyrosinase activity, a core component in melanin biosynthesis, is one of the mechanisms of depigmenting agents. Hydroquinone and kojic acid are the examples of well-known whitening agents widely used in both pharmaceutical and cosmetic products. However, their adverse effect issues still needed to be overcome. A recent study showed that p-chlorophenyl benzyl ether (Cl-benz), a new synthetic compound, more strongly inhibited mushroom tyrosinase than kojic acid. In the current study, cytotoxicity, anti-melanogenic activity and anti-tyrosinase activity of Cl-benz were performed in mouse B16F10 melanoma cells compared to kojic acid. After 24 h of treatment on B16F10 cells, the cytotoxicity was not observed with Cl-benz and kojic acid. However, after incubation for 48 h, kojic acid at a concentration of 500 μM reduced cell viability less than 50%, whereas Cl-benz-treated cells showed negligible cytotoxicity. For cell-based assay, Cl-benz exhibited inhibitory effect similar to kojic acid. Melanin production in B16F10 cells was suppressed by Cl-benz in a dose dependent manner. One hundred micrograms of Cl-benz decreased melanin content in α-MSH by 66%. Moreover, the percentage of cellular tyrosinase activity of Cl-benz showed positive association with its corresponding melanin content. These results revealed that Cl-benz could inhibit melanogenesis via the mechanism of cellular tyrosinase inhibition. Accordingly, Cl-benz has potential to become a novel skin whitening agent in terms of efficacy and safety.

scholarly journals Activation of MITF by Argan Oil Leads to the Inhibition of the Tyrosinase and Dopachrome Tautomerase Expressions in B16 Murine Melanoma Cells

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Myra O. Villareal ◽  
Sayuri Kume ◽  
Thouria Bourhim ◽  
Fatima Zahra Bakhtaoui ◽  
Kenichi Kashiwagi ◽  
...  
Keyword(s):  
Therapeutic Potential ◽  
Inhibitory Effect ◽  
Melanoma Cells ◽  
Scientific Basis ◽  
Melanin Biosynthesis ◽  
Dopachrome Tautomerase ◽  
Murine Melanoma ◽  
Argan Oil ◽  
Dose Dependent Decrease ◽  
Murine Melanoma Cells

Argan (Argania spinosaL.) oil has been used for centuries in Morocco as cosmetic oil to maintain a fair complexion and to cure skin pimples and chicken pox pustules scars. Although it is popular, the scientific basis for its effect on the skin has not yet been established. Here, the melanogenesis regulatory effect of argan oil was evaluated using B16 murine melanoma cells. Results of melanin assay using B16 cells treated with different concentrations of argan oil showed a dose-dependent decrease in melanin content. Western blot results showed that the expression levels of tyrosinase (TYR), tyrosinase-related protein 1 (TRP1), and dopachrome tautomerase (DCT) proteins were decreased. In addition, there was an increase in the activation of MITF and ERK1/2. Real-time PCR results revealed a downregulation ofTyr,Trp1,Dct, andMitfmRNA expressions. Argan oil treatment causes MITF phosphorylation which subsequently inhibited the transcription of melanogenic enzymes, TYR and DCT. The inhibitory effect of argan oil on melanin biosynthesis may be attributed to tocopherols as well as the synergistic effect of its components. The results of this study provide the scientific basis for the traditionally established benefits of argan oil and present its therapeutic potential against hyperpigmentation disorders.

Inhibitory Effect of Isoprenoid-Substituted Flavonoids Isolated fromArtocarpus heterophylluson Melanin Biosynthesis

Planta Medica ◽  
2006 ◽  
Vol 72 (09) ◽  
pp. 847-850 ◽  
Author(s):  
Enos Arung ◽  
Kuniyoshi Shimizu ◽  
Ryuichiro Kondo
Keyword(s):  
Inhibitory Effect ◽  
Melanin Biosynthesis
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