Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants

10.3791/61517 ◽  
2020 ◽  
Author(s):  
Lim Chee Liew ◽  
Yan Wang ◽  
Marta Peirats-Llobet ◽  
Oliver Berkowitz ◽  
James Whelan ◽  
...  
Keyword(s):  
Gene Expression ◽  
Rna Sequencing ◽  
Expression Analysis ◽  
Laser Capture Microdissection ◽  
Gene Expression Analysis ◽  
Specific Gene ◽  
Tissue Specific ◽  
Tissue Specific Gene ◽  
Specific Gene Expression ◽  
Laser Capture

scholarly journals RNA-sequencing across three matched tissues reveals shared and tissue-specific gene expression and pathway signatures of COPD

2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Jarrett D. Morrow ◽  
Robert P. Chase ◽  
Margaret M. Parker ◽  
Kimberly Glass ◽  
Minseok Seo ◽  
...  
Keyword(s):  
Gene Expression ◽  
Rna Sequencing ◽  
Specific Gene ◽  
Tissue Specific ◽  
Tissue Specific Gene ◽  
Specific Gene Expression

scholarly journals Tissue-specific gene expression analysis of silkworm (Bombyx mori) by quantitative real-time RT-PCR

BMB Reports ◽  
2010 ◽  
Vol 43 (7) ◽  
pp. 480-484 ◽  
Author(s):  
Seung-Won Park ◽  
Seok-Woo Kang ◽  
Tae-Won Goo ◽  
Seong-Ryul Kim ◽  
Gwang-Gill Lee ◽  
...  
Keyword(s):  
Gene Expression ◽  
Bombyx Mori ◽  
Real Time ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Specific Gene ◽  
Rt Pcr ◽  
Tissue Specific ◽  
Tissue Specific Gene ◽  
Silkworm Bombyx Mori

Histones: genetic diversity and tissue-specific gene expression

1997 ◽  
Vol 107 (1) ◽  
pp. 1-10 ◽  
Author(s):  
D. Doenecke ◽  
W. Albig ◽  
C. Bode ◽  
B. Drabent ◽  
K. Franke ◽  
...  
Keyword(s):  
Gene Expression ◽  
Genetic Diversity ◽  
Specific Gene ◽  
Tissue Specific ◽  
Tissue Specific Gene ◽  
Specific Gene Expression

scholarly journals Blood—Brain Barrier Genomics

2001 ◽  
Vol 21 (1) ◽  
pp. 61-68 ◽  
Author(s):  
Jian Yi Li ◽  
Ruben J. Boado ◽  
William M. Pardridge
Keyword(s):  
Gene Expression ◽  
Blood Brain Barrier ◽  
Brain Barrier ◽  
Specific Gene ◽  
Microvascular Endothelium ◽  
Tissue Specific ◽  
Tissue Specific Gene ◽  
Specific Gene Expression ◽  
Blood Brain ◽  
Tester Cdna

The blood–brain barrier (BBB) is formed by the brain microvascular endothelium, and the unique transport properties of the BBB are derived from tissue-specific gene expression within this cell. The current studies developed a gene microarray approach specific for the BBB by purifying the initial mRNA from isolated rat brain capillaries to generate tester cDNA. A polymerase chain reaction–based subtraction cloning method, suppression subtractive hybridization (SSH), was used, and the BBB cDNA was subtracted with driver cDNA produced from mRNA isolated from rat liver and kidney. Screening 5% of the subtracted tester cDNA resulted in identification of 50 gene products and more than 80% of those were selectively expressed at the BBB; these included novel gene sequences not found in existing databases, ESTs, and known genes that were not known to be selectively expressed at the BBB. Genes in the latter category include tissue plasminogen activator, insulin-like growth factor-2, PC-3 gene product, myelin basic protein, regulator of G protein signaling 5, utrophin, IκB, connexin-45, the class I major histocompatibility complex, the rat homologue of the transcription factors hbrm or EZH1, and organic anion transporting polypeptide type 2. Knowledge of tissue-specific gene expression at the BBB could lead to new targets for brain drug delivery and could elucidate mechanisms of brain pathology at the microvascular level.

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