Bacteriophages of Pseudomonas tolaasii for the Biological Control of Brown Blotch Disease

2011 ◽  
Vol 54 (1) ◽  
Author(s):  
Min-Hee Kim
Keyword(s):  
Biological Control ◽  
Pseudomonas Tolaasii ◽  
Brown Blotch ◽  
Brown Blotch Disease

Characterization of bacteriophage ϕPto-bp6g, a novel phage that lyses Pseudomonas tolaasii causing brown blotch disease in mushrooms

2012 ◽  
Vol 91 (3) ◽  
pp. 514-519 ◽  
Author(s):  
Hanh Thi Dieu Nguyen ◽  
Soojin Yoon ◽  
Min-Hee Kim ◽  
Young-Kee Kim ◽  
Moon-Young Yoon ◽  
...  
Keyword(s):  
Pseudomonas Tolaasii ◽  
Brown Blotch ◽  
Brown Blotch Disease

scholarly journals Resistance Sources to Brown Blotch Disease (Pseudomonas tolaasii) in a Diverse Collection of Pleurotus Mushroom Strains

Pathogens ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 227 ◽  
Author(s):  
Benjamin Azu Okorley ◽  
Frederick Leo Sossah ◽  
Dan Dai ◽  
Shuai Xu ◽  
Zhenghui Liu ◽  
...  
Keyword(s):  
Pseudomonas Tolaasii ◽  
Resistance Sources ◽  
Controlled Conditions ◽  
Resistant Strains ◽  
Brown Blotch ◽  
Brown Blotch Disease ◽  
Moderately Resistant

Brown blotch disease (BBD) caused by Pseudomonas tolaasii is one of the most devastating diseases of Pleurotus spp. worldwide. Breeding for resistant strains is the most effective method for controlling BBD. To identify resistant germplasm for BBD management, 97 strains comprising 21 P. cf. floridanus, 20 P. ostreatus, and 56 P. pulmonarius were screened by two different methods; namely, inoculation of the pathogen on the mushroom pileus (IMP) and on the spawned substrate (IMSS) under controlled conditions. Out of the 97 strains screened, 22 P. pulmonarius, and four P. cf. floridanus were moderately resistant to BBD using the IMP method. Eleven P. pulmonarius, six P. cf. florida, and one P. ostreatus strains were highly resistant to BBD using the IMSS method. All of the 97 strains showed varying degrees of susceptibility using the IMP method, but eight strains were completely resistant using the IMSS method. Combining these two methods, five strains were highly resistant (four P. pulmonarius and one P. cf. floridanus) and 11 were moderately resistant (eight P. pulmonarius and three P. cf. floridanus). The resistance sources to P. tolaasii identified in P. pulmonarius and P. cf. floridanus could be used for further breeding of Pleurotus spp.

scholarly journals Insights into detoxification of tolaasins, the toxins behind mushroom bacterial blotch, by Microbacterium foliorum NBRC 103072T

2021 ◽  
Author(s):  
Shun Tomita ◽  
Akinobu Kajikawa ◽  
Shizunobu Igimi ◽  
Hirosuke Shinohara ◽  
Kenji Yokota
Keyword(s):  
Degradation Process ◽  
Detergent Solution ◽  
Bacterial Cells ◽  
Biological Degradation ◽  
Pseudomonas Tolaasii ◽  
Cyclic Lipopeptides ◽  
Peptide Moiety ◽  
Brown Blotch ◽  
Brown Blotch Disease ◽  
Detoxification Process

Tolaasins are lipodepsipeptides secreted by Pseudomonas tolaasii, the causal agent of brown blotch disease of mushrooms, and are the toxins that cause the brown spots. We previously reported that Microbacterium foliorum NBRC 103072T is an effective tolaasin-detoxifying bacterium. In this study, we aimed to characterize the tolaasin-detoxification process of M. foliorum NBRC 103072T. The tolaasin-detoxification by M. foliorum NBRC 103072T was carried out by hydrolyzation of tolaasins at two specific sites in the peptide moiety of tolaasins by its cells, and the resulting fragments were released from bacterial cells. The tolaasin-hydrolyzing activity can be extracted by neutral detergent solution from M. foliorum NBRC 103072T cells. Moreover, tolaasin-adsorption to the bacterial cells occurred prior to hydrolyzation of tolaasins, which might contribute to the effective tolaasin-detoxification by M. foliorum NBRC 103072T. It is notable that the tolaasin-degradation process by M. foliorum NBRC 103072T is carried out by hydrolyzation at specific sites in the peptide moiety of lipopeptide by bacterial cells as a novel biological degradation process of cyclic lipopeptides.

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