scholarly journals 3D microfabrication of complex structures for biomedical applications via combination of subtractive/additive direct laser writing and 3D printing

2016 ◽  
Author(s):  
Linas Jonušauskas ◽  
Sima Rekštytė ◽  
Edvinas Skliutas ◽  
Simas Butkus ◽  
Mangirdas Malinauskas
Keyword(s):  
Biomedical Applications ◽  
Complex Structures ◽  
Direct Laser Writing ◽  
Laser Material Processing ◽  
Laser Writing ◽  
3D Microstructure ◽  
Direct Laser ◽  
3D Printers

In this work we present current progress on employing direct laser writing (DLW) for creation of 3D microstructures for biomedical applications. Both subtractive and additive variations of DLW allow fabricating structures for in vitro bioanalysis and in vivo tissue engineering. Furthermore, we show that efficiency of 3D microstructure manufacturing can be enhanced by combining femtosecond laser material processing with commercial 3D printers.

Fabrication of 3D micro-structured scaffolds by direct laser writing in pre-polymers for in vitro and in vivo studies

2017 ◽  
Author(s):  
Sima Rekštytė ◽  
Justinas Mačiulaitis ◽  
Romaldas Mačiulaitis ◽  
Mangirdas Malinauskas
Keyword(s):  
In Vivo Studies ◽  
Direct Laser Writing ◽  
Laser Writing ◽  
Direct Laser

scholarly journals Self-assembling peptides and their applications in tissue engineering

2018 ◽  
Author(s):  
Graziano Deidda
Keyword(s):  
Tissue Engineering ◽  
Direct Laser Writing ◽  
Laser Writing ◽  
Self Assembling ◽  
Direct Laser

Τα πρωτεϊνικά ικριώματα τα οποία σχηματίζονται από αυτοοργανωμένα πεπτίδια μπορούν να χρησιμοποιηθούν ως εναλλακτικά των πρωτεϊνών της εξωκυττάριας μήτρας (ECM) και θα μπορούσαν να βρουν εφαρμογή στην αναγεννητική ιατρική. Στο πρώτο κεφάλαιο της παρούσας διατριβής ένα πεπτίδιο που περιέχει την αμυλοειδή αλληλουχία NSGAITIG η οποία έχει ανακτηθεί από το μίσχο της πρωτεΐνης, ίνας του αδενοϊού ανασχεδιάστηκε για την κατασκευή τρισδιάστατων (3D) ικριωμάτων ανθρώπινου ιστού και μοντέλων κυτταρικής καλλιέργειας in vitro. Εν συνεχεία αυτή η ακολουθία μελετήθηκε έτσι ώστε να διαμορφωθεί ένα εντεκαπεπτίδιο το οποίο περιέχει το μοτίβο RGD στο αμινοτελικό άκρο και ένα κομβικό αμινοξύ όπως η κυστεΐνη στο καρβοξυτελικό άκρο, έτσι ώστε να σχηματιστεί η αλληλουχία RGDSGAITIGC ως τελικό προϊόν. Το αμυλοειδές αυτό πεπτίδιο μπορεί να παίξει έναν πολλά υποσχόμενο διλειτουργικό ρόλο ως βιολογικό ικρίωμα καθώς επάγει την κυτταρική προσκόλληση και λόγω του αμινοξέα της κυστεΐνης μπορεί να χρησιμοποιηθεί ως ενδιάμεσος προσδέτης βιομορίων. Εναλλακτικά, στο δεύτερο κεφάλαιο, φωτοευαίσθητα κατάλοιπα όπως η τυροσίνη ή η τρυπτοφάνη σχεδιάστηκαν για να εισαχθούν στο καρβοξυτελικό άκρο ώστε να δώσουν τις αλληλουχίες RGDSGAITIGY και RGDSGAITIGW. Αυτά τα κατάλοιπα μπορούν να πυροδοτήσουν ένα φαινόμενο πολυφωτονικού πολυμερισμού (MPP/MPA) μέσω της χρήσης της τεχνικής της Direct Laser Writing (DLW) με απώτερο στόχο την εφαρμογή σε υψηλά ανταποκρινόμενες βιολογικές θεραπείες, όπως η αναγέννηση του μυοκαρδίου. Εν συνεχεία, στο τρίτο κεφάλαιο, τα πεπτιδικά παράγωγα τα οποία χαρακτηρίστηκαν προηγουμένως, μελετήθηκαν ως υποστρώματα στη θεραπεία βλαστοκυττάρων. Επιπλέον τροποποιημένα εμβρυϊκά βλαστοκύτταρα μελετήθηκαν για κυτταρική προσκόλληση και πολλαπλασιασμό. Επιπροσθέτως, η μελέτη των ρεολογικών ιδιοτήτων όλων των υποστρωμάτων και η περαιτέρω αξιολόγηση της λειτουργικής έγχυσης μελετήθηκε με σκοπό την προοπτική θεραπευτικής χρησιμοποίησής τους in situ.

Expansion of direct laser writing (DLW) capabilities for usage in biomedical applications

2021 ◽  
Author(s):  
Linas Jonušauskas ◽  
Dovile Andrijec ◽  
Deividas Andriukaitis ◽  
Rokas Vargalis ◽  
Tomas Baravykas ◽  
...  
Keyword(s):  
Biomedical Applications ◽  
Direct Laser Writing ◽  
Laser Writing ◽  
Direct Laser

Biological and biomedical applications of collagenous biomaterials

1990 ◽  
Vol 48 (3) ◽  
pp. 856-857
Author(s):  
Yasushi P. Kato ◽  
Michael G. Dunn ◽  
Frederick H. Silver ◽  
Arthur J. Wasserman
Keyword(s):  
Biomedical Applications ◽  
Soft Tissues ◽  
Collagen Fibers ◽  
Bone Collagen ◽  
Cover Slip ◽  
Fibroblast Migration ◽  
Cellular Expression ◽  
Defect Repair

Collagenous biomaterials have been used for growing cells in vitro as well as for augmentation and replacement of hard and soft tissues. The substratum used for culturing cells is implicated in the modulation of phenotypic cellular expression, cellular orientation and adhesion. Collagen may have a strong influence on these cellular parameters when used as a substrate in vitro. Clinically, collagen has many applications to wound healing including, skin and bone substitution, tendon, ligament, and nerve replacement. In this report we demonstrate two uses of collagen. First as a fiber to support fibroblast growth in vitro, and second as a demineralized bone/collagen sponge for radial bone defect repair in vivo.For the in vitro study, collagen fibers were prepared as described previously. Primary rat tendon fibroblasts (1° RTF) were isolated and cultured for 5 days on 1 X 15 mm sterile cover slips. Six to seven collagen fibers, were glued parallel to each other onto a circular cover slip (D=18mm) and the 1 X 15mm cover slip populated with 1° RTF was placed at the center perpendicular to the collagen fibers. Fibroblast migration from the 1 x 15mm cover slip onto and along the collagen fibers was measured daily using a phase contrast microscope (Olympus CK-2) with a calibrated eyepiece. Migratory rates for fibroblasts were determined from 36 fibers over 4 days.

Conjugates of Classical DNA/RNA Binder with Nucleobase: Chemical, Biochemical and Biomedical Applications

2019 ◽  
Vol 26 (30) ◽  
pp. 5609-5624
Author(s):  
Dijana Saftić ◽  
Željka Ban ◽  
Josipa Matić ◽  
Lidija-Marija Tumirv ◽  
Ivo Piantanida
Keyword(s):  
Molecular Weight ◽  
Small Molecules ◽  
Biomedical Applications ◽  
Protein Targets ◽  
New Class ◽  
Rna Targets ◽  
Covalently Linked ◽  
Structural Aspects

: Among the most intensively studied classes of small molecules (molecular weight < 650) in biomedical research are small molecules that non-covalently bind to DNA/RNA, and another intensively studied class is nucleobase derivatives. Both classes have been intensively elaborated in many books and reviews. However, conjugates consisting of DNA/RNA binder covalently linked to nucleobase are much less studied and have not been reviewed in the last two decades. Therefore, this review summarized reports on the design of classical DNA/RNA binder – nucleobase conjugates, as well as data about their interactions with various DNA or RNA targets, and even in some cases protein targets are involved. According to these data, the most important structural aspects of selective or even specific recognition between small molecule and target are proposed, and where possible related biochemical and biomedical aspects were discussed. The general conclusion is that this, rather new class of molecules showed an amazing set of recognition tools for numerous DNA or RNA targets in the last two decades, as well as few intriguing in vitro and in vivo selectivities. Several lead research lines show promising advancements toward either novel, highly selective markers or bioactive, potentially druggable molecules.

scholarly journals Hexapod Assassins’ Potion: Venom Composition and Bioactivity from the Eurasian Assassin Bug Rhynocoris iracundus

Biomedicines ◽  
2021 ◽  
Vol 9 (7) ◽  
pp. 819
Author(s):  
Nicolai Rügen ◽  
Timothy P. Jenkins ◽  
Natalie Wielsch ◽  
Heiko Vogel ◽  
Benjamin-Florian Hempel ◽  
...  
Keyword(s):  
Biomedical Applications ◽  
Galleria Mellonella ◽  
Systemic Reactions ◽  
Venom Composition ◽  
Assassin Bug ◽  
Molecular Components ◽  
First Time ◽  
Tissue Digestion

Assassin bug venoms are potent and exert diverse biological functions, making them potential biomedical goldmines. Besides feeding functions on arthropods, assassin bugs also use their venom for defense purposes causing localized and systemic reactions in vertebrates. However, assassin bug venoms remain poorly characterized. We collected the venom from the assassin bug Rhynocoris iracundus and investigated its composition and bioactivity in vitro and in vivo. It caused lysis of murine neuroblastoma, hepatoma cells, and healthy murine myoblasts. We demonstrated, for the first time, that assassin bug venom induces neurolysis and suggest that it counteracts paralysis locally via the destruction of neural networks, contributing to tissue digestion. Furthermore, the venom caused paralysis and melanization of Galleria mellonella larvae and pupae, whilst also possessing specific antibacterial activity against Escherichia coli, but not Listeria grayi and Pseudomonas aeruginosa. A combinatorial proteo-transcriptomic approach was performed to identify potential toxins responsible for the observed effects. We identified neurotoxic Ptu1, an inhibitory cystin knot (ICK) toxin homologous to ω-conotoxins from cone snails, cytolytic redulysins homologous to trialysins from hematophagous kissing bugs, and pore-forming hemolysins. Additionally, chitinases and kininogens were found and may be responsible for insecticidal and cytolytic activities. We demonstrate the multifunctionality and complexity of assassin bug venom, which renders its molecular components interesting for potential biomedical applications.

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