MTBE-Degradation by Hydrodynamic Induced Cavitation

2012 ◽  
Author(s):  
Andreas Andreas
Keyword(s):  
Mtbe Degradation

Aerobic biodegradation of gasoline oxygenates MTBE and TBA

2001 ◽  
Vol 43 (2) ◽  
pp. 277-284 ◽  
Author(s):  
G. J. Wilson ◽  
A. P. Richter ◽  
M. T. Suidan ◽  
A. D. Venosa
Keyword(s):  
Gel Electrophoresis ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Aerobic Biodegradation ◽  
Batch Experiments ◽  
Gradient Gel Electrophoresis ◽  
Biomass Retention ◽  
Gasoline Oxygenates ◽  
Continuously Stirred Tank Reactor ◽  
The Individual ◽  
Mtbe Degradation

MTBE degradation was investigated using a continuously stirred tank reactor (CSTR) with biomass retention (porous pot reactor) operated under aerobic conditions. MTBE was fed to the reactor at an influent concentration of 150 mg/l (1.70 mmol/l). A second identical reactor was operated as a control under the same conditions with the addition of 2.66 g/l of sodium azide, to kill any biological activity. Results from these experiments suggest that biomass retention is critical to the degradation of MTBE. The rate of MTBE removal was shown to be related to the VSS concentration. MTBE removal exceeded 99.99% when the VSS concentration in the reactor was over 600 mg/l. Results obtained from batch experiments conducted on mixed liquor samples from the porous pot reactor indicate that the individual rates of biodegradation of MTBE and TBA were higher for initial concentrations of 15 mg/l than for concentrations of 5 mg/l. The presence of TBA at lower concentrations did not effect the rate of MTBE degradation, however higher concentrations of TBA did reduce the rate of biodegradation of MTBE. Denaturing Gradient Gel Electrophoresis (DGGE) analysis reveals that the culture consisted of a community of bacterial organisms of about 6 species.

scholarly journals Characterization of co-metabolic biodegradation of methyl tert-butyl ether by a Acinetobacter sp. strain

RSC Advances ◽  
2019 ◽  
Vol 9 (67) ◽  
pp. 38962-38972
Author(s):  
Shanshan Li ◽  
Dan Wang ◽  
Dan Du ◽  
Keke Qian ◽  
Wei Yan
Keyword(s):  
Batch Reactor ◽  
Methyl Tert Butyl Ether ◽  
Fed Batch ◽  
Butyl Ether ◽  
Tert Butyl ◽  
Acinetobacter Sp ◽  
Mtbe Degradation

Acinetobacter sp. SL3 could co-metabolically degrade MTBE when grown on n-alkanes. An extremely low TBA accumulation were achieved on n-octane. The fed-batch reactor degradation revealed continuous MTBE degradation capacity by Acinetobacter sp. SL3.

scholarly journals Biodegradation of Methyl tert-Butyl Ether by a Pure Bacterial Culture

2001 ◽  
Vol 67 (12) ◽  
pp. 5601-5607 ◽  
Author(s):  
Paul B. Hatzinger ◽  
Kevin McClay ◽  
Simon Vainberg ◽  
Marina Tugusheva ◽  
Charles W. Condee ◽  
...  
Keyword(s):  
Bacterial Culture ◽  
Butyl Alcohol ◽  
The Other ◽  
Methyl Tert Butyl Ether ◽  
Butyl Ether ◽  
Pure Bacterial Culture ◽  
Degradation Activity ◽  
Type Strains ◽  
Mtbe Degradation ◽  
Allyl Thiourea

ABSTRACT Biodegradation of methyl tert-butyl ether (MTBE) by the hydrogen-oxidizing bacterium Hydrogenophaga flavaENV735 was evaluated. ENV735 grew slowly on MTBE ortert-butyl alcohol (TBA) as sole sources of carbon and energy, but growth on these substrates was greatly enhanced by the addition of a small amount of yeast extract. The addition of H2 did not enhance or diminish MTBE degradation by the strain, and MTBE was only poorly degraded or not degraded by type strains of Hydrogenophaga or hydrogen-oxidizing enrichment cultures, respectively. MTBE degradation activity was constitutively expressed in ENV735 and was not greatly affected by formaldehyde, carbon monoxide, allyl thiourea, or acetylene. MTBE degradation was inhibited by 1-amino benzotriazole and butadiene monoepoxide. TBA degradation was inducible by TBA and was inhibited by formaldehyde at concentrations of >0.24 mM and by acetylene but not by the other inhibitors tested. These results demonstrate that separate, independently regulated genes encode MTBE and TBA metabolism in ENV735.

scholarly journals Metabolism and Cometabolism of Cyclic Ethers by a Filamentous Fungus, a Graphium sp.

2009 ◽  
Vol 75 (17) ◽  
pp. 5514-5522 ◽  
Author(s):  
Kristin Skinner ◽  
Lynda Cuiffetti ◽  
Michael Hyman
Keyword(s):  
Diethyl Ether ◽  
Filamentous Fungus ◽  
Methyl Formate ◽  
Lag Phase ◽  
Cyclic Ethers ◽  
Butyl Ether ◽  
Alcohol Dehydrogenases ◽  
Vigorous Growth ◽  
Mtbe Degradation

ABSTRACT The filamentous fungus Graphium sp. (ATCC 58400) grows on gaseous n-alkanes and diethyl ether. n-Alkane-grown mycelia of this strain also cometabolically oxidize the gasoline oxygenate methyl tert -butyl ether (MTBE). In this study, we characterized the ability of this fungus to metabolize and cometabolize a range of cyclic ethers, including tetrahydrofuran (THF) and 1,4-dioxane (14D). This strain grew on THF and other cyclic ethers, including tetrahydropyran and hexamethylene oxide. However, more vigorous growth was consistently observed on the lactones and terminal diols potentially derived from these ethers. Unlike the case in all previous studies of microbial THF oxidation, a metabolite, γ-butyrolactone, was observed during growth of this fungus on THF. Growth on THF was inhibited by the same n-alkenes and n-alkynes that inhibit growth of this fungus on n-alkanes, while growth on γ-butyrolactone or succinate was unaffected by these inhibitors. Propane and THF also behaved as mutually competitive substrates, and propane-grown mycelia immediately oxidized THF, without a lag phase. Mycelia grown on propane or THF exhibited comparable high levels of hemiacetal-oxidizing activity that generated methyl formate from mixtures of formaldehyde and methanol. Collectively, these observations suggest that THF and n-alkanes may initially be oxidized by the same monooxygenase and that further transformation of THF-derived metabolites involves the activity of one or more alcohol dehydrogenases. Both propane- and THF-grown mycelia also slowly cometabolically oxidized 14D, although unlike THF oxidation, this reaction was not sustainable. Specific rates of THF, 14D, and MTBE degradation were very similar in THF- and propane-grown mycelia.

Chemical destruction of MTBE using Fenton's Reagent: effect of ferrous iron/hydrogen peroxide ratio

2003 ◽  
Vol 47 (9) ◽  
pp. 165-171 ◽  
Author(s):  
A. Burbano ◽  
D. Dionysiou ◽  
M. Suidan ◽  
T. Richardson
Keyword(s):  
Hydrogen Peroxide ◽  
Ferrous Iron ◽  
Degradation Process ◽  
Methyl Tert Butyl Ether ◽  
Molar Ratio ◽  
Limiting Factor ◽  
Fenton’S Reagent ◽  
Butyl Ether ◽  
Fenton's Reagent ◽  
Mtbe Degradation

In previous laboratory experiments Fenton's Reagent (FR) was successfully used as the source of hydroxyl radicals (OH•) for chemical treatment of low concentrations of methyl tert-butyl ether (MTBE) in water. Although under certain conditions MTBE degradation levels as high as 99.99% were achieved, none of these experiments resulted in complete MTBE mineralization. In all cases, these experiments applied FR as an equimolar concentration of ferrous iron (Fe2+) and hydrogen peroxide (H2O2). The present study investigates the effect of H2O2/Fe2+ molar ratio on the extent of degradation of MTBE and intermediate products in water at pH = 3.0. The initial concentration of MTBE studied was 0.0227 mM (approximately 2 mg/L). Initially, the dose of Fe2+ was kept constant at a Fe2+/MTBE molar ratio of 10:1 and the dose of H2O2 was varied to achieve different H2O2/Fe2+ molar ratios. The results revealed that higher degradation efficiency was achieved when FR was used as an equimolar mixture (H2O2/Fe2+ molar ratio = 1.0). The extent of MTBE degradation decreased when the H2O2/Fe2+ molar ratio was changed to values higher or lower than 1.0. These results suggest that a stoichiometric relationship (1:1) between the FR components optimizes the degradation process for this reactant system. It is hypothesized that an excess of H2O2 enhances the effect of reactions that scavenge OH•, while a decreased amount of H2O2 would be a limiting factor for the Fenton Reaction.

scholarly journals Naturally Occurring Bacteria Similar to the Methyl tert-Butyl Ether (MTBE)-Degrading Strain PM1 Are Present in MTBE-Contaminated Groundwater

2003 ◽  
Vol 69 (5) ◽  
pp. 2616-2623 ◽  
Author(s):  
Krassimira Hristova ◽  
Binyam Gebreyesus ◽  
Douglas Mackay ◽  
Kate M. Scow
Keyword(s):  
Dna Sequences ◽  
Field Studies ◽  
Methyl Tert Butyl Ether ◽  
Butyl Ether ◽  
Naturally Occurring ◽  
Degrading Bacteria ◽  
Pure Cultures ◽  
Air Force Base ◽  
Mtbe Degradation

ABSTRACT Methyl tert-butyl ether (MTBE) is a widespread groundwater contaminant that does not respond well to conventional treatment technologies. Growing evidence indicates that microbial communities indigenous to groundwater can degrade MTBE under aerobic and anaerobic conditions. Although pure cultures of microorganisms able to degrade or cometabolize MTBE have been reported, to date the specific organisms responsible for MTBE degradation in various field studies have not be identified. We report that DNA sequences almost identical (99% homology) to those of strain PM1, originally isolated from a biofilter in southern California, are naturally occurring in an MTBE-polluted aquifer in Vandenberg Air Force Base (VAFB), Lompoc, California. Cell densities of native PM1 (measured by TaqMan quantitative PCR) in VAFB groundwater samples ranged from below the detection limit (in anaerobic sites) to 103 to 104 cells/ml (in oxygen-amended sites). In groundwater from anaerobic or aerobic sites incubated in microcosms spiked with 10 μg of MTBE/liter, densities of native PM1 increased to approximately 105 cells/ml. Native PM1 densities also increased during incubation of VAFB sediments during MTBE degradation. In controlled field plots amended with oxygen, artificially increasing the MTBE concentration was followed by an increase in the in situ native PM1 cell density. This is the first reported relationship between in situ MTBE biodegradation and densities of MTBE-degrading bacteria by quantitative molecular methods.

scholarly journals Use of omic tools to assess methyl tert-butyl ether (MTBE) degradation in groundwater

2019 ◽  
Vol 378 ◽  
pp. 120618 ◽  
Author(s):  
Katarzyna H. Kucharzyk ◽  
Heather V. Rectanus ◽  
Craig M. Bartling ◽  
Steve Rosansky ◽  
Angela Minard-Smith ◽  
...  
Keyword(s):  
Methyl Tert Butyl Ether ◽  
Butyl Ether ◽  
Tert Butyl ◽  
Mtbe Degradation

scholarly journals Carbon Isotope Fractionation during Anaerobic Degradation of Methyl tert-Butyl Ether under Sulfate-Reducing and Methanogenic Conditions

2006 ◽  
Vol 72 (2) ◽  
pp. 1157-1163 ◽  
Author(s):  
Piyapawn Somsamak ◽  
Hans H. Richnow ◽  
Max M. Häggblom
Keyword(s):  
Carbon Isotope ◽  
Anaerobic Degradation ◽  
Isotope Fractionation ◽  
Isotope Analysis ◽  
Methyl Tert Butyl Ether ◽  
Public Attention ◽  
Butyl Ether ◽  
Carbon Isotope Fractionation ◽  
Sulfate Reducing ◽  
Mtbe Degradation

ABSTRACT Methyl tert-butyl ether (MTBE), an octane enhancer and a fuel oxygenate in reformulated gasoline, has received increasing public attention after it was detected as a major contaminant of water resources. Although several techniques have been developed to remediate MTBE-contaminated sites, the fate of MTBE is mainly dependent upon natural degradation processes. Compound-specific stable isotope analysis has been proposed as a tool to distinguish the loss of MTBE due to biodegradation from other physical processes. Although MTBE is highly recalcitrant, anaerobic degradation has been demonstrated under different anoxic conditions and may be an important process. To accurately assess in situ MTBE degradation through carbon isotope analysis, carbon isotope fractionation during MTBE degradation by different cultures under different electron-accepting conditions needs to be investigated. In this study, carbon isotope fractionation during MTBE degradation under sulfate-reducing and methanogenic conditions was studied in anaerobic cultures enriched from two different sediments. Significant enrichment of 13C in residual MTBE during anaerobic biotransformation was observed under both sulfate-reducing and methanogenic conditions. The isotopic enrichment factors (ε) estimated for each enrichment were almost identical (−13.4 to −14.6; r 2 = 0.89 to 0.99). A ε value of −14.4 ± 0.7 was obtained from regression analysis (r 2 = 0.97, n = 55, 95% confidence interval), when all data from our MTBE-transforming anaerobic cultures were combined. The similar magnitude of carbon isotope fractionation in all enrichments regardless of culture or electron-accepting condition suggests that the terminal electron-accepting process may not significantly affect carbon isotope fractionation during anaerobic MTBE degradation.

scholarly journals Biodegradation of Ether Pollutants by Pseudonocardia sp. Strain ENV478

2006 ◽  
Vol 72 (8) ◽  
pp. 5218-5224 ◽  
Author(s):  
Simon Vainberg ◽  
Kevin McClay ◽  
Hisako Masuda ◽  
Duane Root ◽  
Charles Condee ◽  
...  
Keyword(s):  
Yeast Extract ◽  
Degradation Product ◽  
Suspended Solids ◽  
Total Suspended Solids ◽  
Butyl Alcohol ◽  
Butyl Ether ◽  
Degradation Rates ◽  
Degrading Bacteria ◽  
Contaminated Aquifers ◽  
Mtbe Degradation

ABSTRACT A bacterium designated Pseudonocardia sp. strain ENV478 was isolated by enrichment culturing on tetrahydrofuran (THF) and was screened to determine its ability to degrade a range of ether pollutants. After growth on THF, strain ENV478 degraded THF (63 mg/h/g total suspended solids [TSS]), 1,4-dioxane (21 mg/h/g TSS), 1,3-dioxolane (19 mg/h/g TSS), bis-2-chloroethylether (BCEE) (12 mg/h/g TSS), and methyl tert-butyl ether (MTBE) (9.1 mg/h/g TSS). Although the highest rates of 1,4-dioxane degradation occurred after growth on THF, strain ENV478 also degraded 1,4-dioxane after growth on sucrose, lactate, yeast extract, 2-propanol, and propane, indicating that there was some level of constitutive degradative activity. The BCEE degradation rates were about threefold higher after growth on propane (32 mg/h/g TSS) than after growth on THF, and MTBE degradation resulted in accumulation of tert-butyl alcohol. Degradation of 1,4-dioxane resulted in accumulation of 2-hydroxyethoxyacetic acid (2HEAA). Despite its inability to grow on 1,4-dioxane, strain ENV478 degraded this compound for >80 days in aquifer microcosms. Our results suggest that the inability of strain ENV478 and possibly other THF-degrading bacteria to grow on 1,4-dioxane is related to their inability to efficiently metabolize the 1,4-dioxane degradation product 2HEAA but that strain ENV478 may nonetheless be useful as a biocatalyst for remediating 1,4-dioxane-contaminated aquifers.

Widespread Potential for Microbial MTBE Degradation in Surface-Water Sediments

2001 ◽  
Vol 35 (4) ◽  
pp. 658-662 ◽  
Author(s):  
Paul M. Bradley ◽  
James E. Landmeyer ◽  
Francis H. Chapelle
Keyword(s):  
Surface Water ◽  
Mtbe Degradation
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