Abstract
We have obtained a single spore isolate of Pasteuria penetrans, derived by
allowing a single spore to attach to a secondstage juvenile (J2) of the
root-knot nematode Meloidogyne javanica. By analysing DNA sequences at three
different loci we have obtained evidence that the isolate is, indeed,
genetically pure. We compared the ability of the single spore isolate and
the parent population from which it was selected to attach to and parasitise
both the original population of M. javanica on which it was isolated and a
single egg mass line derived from it. There was no difference in the
attachment of spores of the single spore isolate to juveniles compared to
the parental population, although there were higher numbers of both
attaching to J2 of the single egg mass line compared to its parental
population. Judging from the numbers of egg masses and Pasteuria -infected
females, the single spore isolate was less pathogenic to the parental
population of M. javanica than was the parental spore population.