Selection of the Most Suitable Host for the Mass Production of Pasteuria penetrans an Obligate Parasite of Root-knot Nematode Meloidogyne javanica

2002 ◽  
Vol 2 (11) ◽  
pp. 725-726
Author(s):  
Nazir Javed . ◽  
H.U. Khan . ◽  
Riaz Ahmad .
Nematology ◽  
2004 ◽  
Vol 6 (4) ◽  
pp. 463-471 ◽  
Author(s):  
James R. Trotter ◽  
Daim Ali Darban ◽  
Simon R. Gowen ◽  
Alistair H. Bishop ◽  
Barbara Pembroke

Abstract We have obtained a single spore isolate of Pasteuria penetrans, derived by allowing a single spore to attach to a secondstage juvenile (J2) of the root-knot nematode Meloidogyne javanica. By analysing DNA sequences at three different loci we have obtained evidence that the isolate is, indeed, genetically pure. We compared the ability of the single spore isolate and the parent population from which it was selected to attach to and parasitise both the original population of M. javanica on which it was isolated and a single egg mass line derived from it. There was no difference in the attachment of spores of the single spore isolate to juveniles compared to the parental population, although there were higher numbers of both attaching to J2 of the single egg mass line compared to its parental population. Judging from the numbers of egg masses and Pasteuria -infected females, the single spore isolate was less pathogenic to the parental population of M. javanica than was the parental spore population.


1968 ◽  
Vol 21 (1) ◽  
pp. 21 ◽  
Author(s):  
N Collis-George ◽  
HR Wallace

Variability in hatching of the root�knot nematode M. javanica was reduced by selection of egg sacs of uniform colour, size, and age. Oxygen consumption and hatching rates in a Warburg experiment were compared with hatching rates in dilute agar gel systems involving supply of oxygen by diffusion. Mathematical solutions of oxygen supply for the diffusion systems are possible. Analysis of experiment and of simplified theory suggests that the isolated nematode egg sac or egg has a floating oxygen potential at its surface. For the systems tested there is apparently a linear relationship between cumulative exposure to oxygen concentration and cumulative hatch in both experiment and theory.


1980 ◽  
Vol 55 (3) ◽  
pp. 495-498 ◽  
Author(s):  
D. J. Bagyaraj ◽  
A. Manjunath

1958 ◽  
Vol 9 (2) ◽  
pp. 182 ◽  
Author(s):  
JE Giles ◽  
EM Hutton

Two tomato hybrids bred at the Hawaiian Experiment Station for resistance to Meloidogyne incognita (Kofoid & Thite) Chitmood proved also to be resistant to M. javanica, which is dominant in the Murray Valley and subtropical areas of Australia. These hybrids also possessed resistance to a range of Australian isolates of the fusarium wilt fungus (Fusarium bulbigenum var. lycopersicli(Brushi) Wollenweber & Reinking). Both these important characters were heritable and could be recombined with agronomic quality in hybrids. Fusarium wilt resistance showed positive potence in the progenies. Root-knot nematode resistance had an irregular pattern of inheritance. The potential of varieties as parents was first gauged from simple crosses. The desirable characters were then concentrated in progenies by a modified backoross followed by selfing and successive selection. Yielding ability has been maintained and suitable agronomic characters have been achieved in the hybrids by the breeding methods used. The work has indicated that suitable crop rotation will be necessary to prevent the selection of nematode strains capable of causing severe infection.


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