Sub-Acute Toxicity Study of Fixed Dose Combination of Sulbactomax (Ceftriaxone-Sulbactam) in Swiss Albino Mice and Wistar Rat

The recent study was planned to determine the acute toxicity study of Apium leptophyllum pers. A single dose of 400,1200,2000 mg/ kg of ethanolic extract of Apium leptophyllum pers according to the OECD guidelines and the dose level was administrated orally into swiss albino mice. Oral administration of maximum level of ethanolic extract of Apium leptophyllum pers upto 2000 mg/kg body weight to experimental group of albino mice was evaluated. Biochemical, haematological and histopathological parameters were checked throughout the study. Signs of toxicity, mortality and body weight were monitored for 14 days post treatment of Apium leptophyllum pers . There is no substantial variations were noticed in control and treated groups. Result revealed that the ethanolic extract of Apium leptophyllum is safe and no toxicity was caused.

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Sub-Acute Toxicity Study of Calotropis gigantea Latex Extracts in Male Swiss Albino Mice

Toxicology and Forensic Medicine - Open Journal ◽

10.17140/tfmoj-1-109 ◽

2016 ◽

Vol 1 (2) ◽

pp. 54-64 ◽

Cited By ~ 2

Author(s):

Saswata Sanyal ◽

◽

Pralay Maity ◽

Ananya Pradhan ◽

Madhubanti Bepari ◽

...

Keyword(s):

Acute Toxicity ◽

Toxicity Study ◽

Swiss Albino Mice ◽

Calotropis Gigantea ◽

Acute Toxicity Study ◽

Albino Mice

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Acute Toxicity Study of Porang (Amorphophallus oncophyllus) Flour Macerated with Strobilanthes crispus in Wistar Rats

Open Access Macedonian Journal of Medical Sciences ◽

10.3889/oamjms.2021.6813 ◽

2021 ◽

Vol 9 (A) ◽

pp. 976-981

Author(s):

Rizka Qurrota A’yun ◽

Uswatun Hasanah ◽

Hamam Hadi ◽

Mustofa Mustofa ◽

Eva Nurinda ◽

...

Keyword(s):

Acute Toxicity ◽

Bioactive Compound ◽

Urinary Protein ◽

Toxicity Study ◽

Fixed Dose ◽

Oral Toxicity ◽

Biochemical Tests ◽

Serum Glutamic Oxaloacetic Transaminase ◽

Acute Toxicity Study ◽

Strobilanthes Crispus

BACKGROUND: Porang (Amorphophallus oncophyllus) is a local tuber food that high in bioactive compound glucomannan. It uses are limited due to oxalate acid content which poses health risks. Strobilanthes crispus leaves could reduce the level of calcium oxalate in porang. However, there is still no study to prove its safety. AIM: This study aimed to investigate the acute oral toxicity study of porang (A. oncophyllus) macerated with S. crispus based on observation of mortality rate (LD50), the changes in behavior during 72 h, renal and hepatic function such as urinary protein, serum glutamic oxaloacetic transaminase (SGOT), and serum glutamic pyruvic transaminase (SGPT) levels of Wistar rat (Rattus norvegicus) METHODS: An acute toxicity test was conducted based on the Organization of Economic Co-Operation and Development 420 Fixed-Dose Procedure Guideline that consists of preliminary and main studies. For the preliminary study, rats were divided into control and five treatment groups with the dosage of 50, 300, 2000, and 5000 mg/kg body weight (BW) for each natural porang flour (NPF) and S. crispus-macerated porang flour (SPF). For the main study, rats were divided into four groups, those were NPF and SPF with the dosage of 2000 and 5000 mg/kg BW. Levels of urinary protein and blood serum SGOT and SGPT levels were measured at 0, 24, and 72 after treatment. RESULTS: The acute toxicity study showed that porang and porang macerated with S. crispus were not toxic until the highest dose of 5000 mg/kg BW. It was proved by the absence of LD50, no change in behavior, no weight losses, and also the results of biochemical tests, such as urinary protein, SGOT, and SGPT which were still in the normal range. CONCLUSIONS: Porang flour and SPF were concluded as non-toxic food based on acute toxicity study.

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RESEARCH OF ACUTE TOXICITY OF FIXED DOSE COMBINATION TABLETS OF CITICOLINE AND MEMANTINE

"Medical & pharmaceutical journal "Pulse" ◽

10.26787/nydha-2686-6838-2021-23-7-49-54 ◽

2021 ◽

pp. 49-54

Author(s):

Salakhetdinov D.K. ◽

Sysuev B.B.

Keyword(s):

Acute Toxicity ◽

Clinical Signs ◽

Fixed Dose Combination ◽

Fixed Dose ◽

Intragastric Administration ◽

Test Drug ◽

Modified Release ◽

Dose Combination ◽

Males And Females

The development and study of the fixed dose combination tablets of citicoline and memantine is the relevant problem. The purpose of this study was the assessment of acute toxicity and local irritating effect of the new combined drug, the modified release tablets (citicoline 500 mg + memantine 10 mg). Material of research: fixed dose combination of modified release tablets (citicoline 500 mg + memantine 10 mg) (NovaMedica Innotech, Russia). The tested drug was administered intragastrically to males and females of half-plant outbred rats at a dose of 2000 mg/kg in the amount of active substances. According the research results, the death of animals was not noted. As it was found the value of LD50 for the test drug in the intragastric administration is>5000 mg/kg in accordance with OECD Test No.423. The tested drug can be attributed to the 5th grade of toxicity on the international classification of GHS, because LD50> 5000 mg/kg (V/g) and to 4 class of low-toxic compounds as per Hodge and Sterner classification: 500 <d50 ≤ 5000 mg/kg (V/g). No clinical signs of intoxication in males and females of autumn rats against the background of a single intragastric administration of the test drug was detected visually, There were no changes in animals behavior in the direction of oppression or excitement. The test drug in a single intragastric introduction in a dose of 2000 mg/kg in the amount of active ingredients did not cause a local irritant action. Thus, the drug does not have a toxic and local irritating action in one-time introduction.

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