RAPD-PCR Analysis of Genetic Variation among Isolates of Fusarium graminearum and Fusarium culmorum from Wheat in Adana Turkey

2010 ◽  
Vol 13 (3) ◽  
pp. 138-142 ◽  
Author(s):  
Serife Evrim Aric ◽  
Namik Kemal Koc
Keyword(s):  
Genetic Variation ◽  
Fusarium Graminearum ◽  
Fusarium Culmorum ◽  
Pcr Analysis ◽  
Rapd Pcr

scholarly journals Genetic variation of somaclonal mutants from the 8th generation of Pekalongan accession rodent tuber (typhonium flagelliforme lodd.) based on RAPD-PCR analysis

2018 ◽  
Vol 195 ◽  
pp. 012054
Author(s):  
Ragapadmi Purnamaningsih ◽  
Nesti Fronika Sianipar ◽  
Khoirunnisa Assidqi ◽  
Harco Leslie Hendric Spits Warnars
Keyword(s):  
Genetic Variation ◽  
Pcr Analysis ◽  
Rapd Pcr

RAPD-PCR Analysis of Genetic Variation among Isolates of Alternaria solani and Alternaria alternata from Potato and Tomato

Mycologia ◽  
1998 ◽  
Vol 90 (5) ◽  
pp. 813 ◽  
Author(s):  
Tiffany L. Weir ◽  
David R. Huff ◽  
Barbara J. Christ ◽  
C. Peter Romaine
Keyword(s):  
Genetic Variation ◽  
Alternaria Alternata ◽  
Alternaria Solani ◽  
Pcr Analysis ◽  
Rapd Pcr

scholarly journals VARIASI GENETIK Lactobacillus fermentum Beijerink ASAL SAYUR ASIN BERDASARKAN ANALISIS RFLP 16S-23S rDNA ISR, RAPD-PCR DAN ERIC-PCR

2017 ◽  
Vol 16 (2) ◽  
Author(s):  
Sulistiani Sulistiani ◽  
Wibowo Mangunwardoyo ◽  
Abinawanto Abinawanto ◽  
Endang Sukara ◽  
Achmad Dinoto ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Random Amplified Polymorphic Dna ◽  
Intergenic Spacer ◽  
Molecular Techniques ◽  
Lactobacillus Fermentum ◽  
Pcr Analysis ◽  
Intergenic Spacer Region ◽  
Rapd Pcr ◽  
Upgma Cluster Analysis ◽  
Process Combination

Molecular analysis of Lactobacillus fermentum isolates is essential to understand their genetic variation in relations to their roles in sayur asin fermentation process. Combination of three molecular techniques which is restriction fragment length polymorphism (RFLP) of 16S23S rDNA intergenic spacer region (ISR), random amplified polymorphic DNA (RAPD-PCR) and an enterobacterial repetitive intergenic consensus (ERIC-PCR) analysis were performed to discriminate 19 representative isolates of L. fermentum isolated from sayur asin. The result showed that L. fermentum strain D11 is distantly related to other isolates based on RFLP using HhaI restriction enzyme and RAPDPCR analyses. In addition, both of RAPD-PCR and ERIC-PCR successfully determined the genetic variation among L. fermentum strains by exhibiting distinct 4-8 bands (800-2080 bp) and 4-10 bands (280-3050 bp), respectively. A dendogram generated from UPGMA cluster analysis of both RAPD-PCR and ERIC-PCR data showed two distinct genotypic groups exist among L. fermentum isolated from sayur asin in Indonesia.

scholarly journals Comparative studies about fungal colonization and deoxynivalenol translocation in barley plants inoculated at the base with Fusarium graminearum, Fusarium culmorum and Fusarium pseudograminearum

2018 ◽  
Vol 27 (1) ◽  
Author(s):  
Francesco Pecoraro ◽  
Marta Giannini ◽  
Giovanni Beccari ◽  
Lorenzo Covarelli ◽  
Gianfranco Filippini ◽  
...  
Keyword(s):  
Real Time ◽  
Fusarium Graminearum ◽  
Fusarium Culmorum ◽  
Comparative Investigation ◽  
Crown Rot ◽  
Pcr Analysis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Fungal Colonization ◽  
Stem Base ◽  
Real Time Quantitative Pcr

Fusarium crown rot (FCR), an important disease of wheat and barley, is mainly caused by Fusarium graminearum, F. culmorum and F. pseudograminearum, which are also responsible for mycotoxin production. This is the first comparative investigation of their colonization on barley plants after stem base inoculation. At plant maturity, FCR symptoms were visually evaluated, fungal biomass was quantified by Real-Time quantitative PCR and deoxynivalenol (DON) was detected by enzyme-linked immunosorbent assay (ELISA). All the inoculated strains caused the typical FCR necrotic symptoms. Real-Time PCR analysis showed that F. graminearum and F. culmorum were present in the head tissues, while F. pseudograminearum colonized only up to the area including the second node of the stem. Conversely, DON was detected up to the head for all the three species. This study shows that, as already demonstrated in previous research for wheat, DON may be detected up to the head as a consequence of stem base infection by the three FCR agents

RAPD-PCR analysis of genetic variation among isolates ofAlternaria solaniandAlternaria alternatafrom potato and tomato

Mycologia ◽  
1998 ◽  
Vol 90 (5) ◽  
pp. 813-821 ◽  
Author(s):  
Tiffany L. Weir ◽  
David R. Huff ◽  
Barbara J. Christ ◽  
C. Peter Romaine
Keyword(s):  
Genetic Variation ◽  
Pcr Analysis ◽  
Rapd Pcr

scholarly journals Genetic variation and relationships of Zea mays and Sorghum species using RAPD-PCR and SDS-PAGE of seed proteins

2013 ◽  
Vol 12 (27) ◽  
pp. 4269-4276 ◽  
Author(s):  
Osman ◽  
G ◽  
Munshi ◽  
Altf A ◽  
F ◽  
...  
Keyword(s):  
Zea Mays ◽  
Genetic Variation ◽  
Seed Proteins ◽  
Rapd Pcr ◽  
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