Microbiological Analysis of Solar and Freeze-Dried Jameed Produced from Cow and Sheep Milk with the Addition of Carrageenan Mix to the Jameed Paste

2008 ◽  
Vol 7 (6) ◽  
pp. 726-729 ◽  
Author(s):  
Amer Al Omari ◽  
Jihad M. Quasem ◽  
Ayman Suliman Ma
2021 ◽  
Vol 4 (1) ◽  
pp. 14
Author(s):  
Tatyana Boryanova Balabanova ◽  
Mihaela Georgieva Ivanova

Milk secretion in small ruminants present some particularities for cytoplasmatic particles which are shed in the milk together with leukocytes and epithelial cells. Therefore, a parameter like a somatic cell count is worldwide used as a measurement indicator of subclinical mastitis. The aim of the present study was to establish the change of chemical and microbiological characteristics on the composition of sheep milk, obtained from Pleven Blackhead sheep breed during different stages of lactation. It was established that the chemical characteristics of sheep milk varied widely according to the way animals were raised and fed. The obtained results described a slight increase in the values of the total concentration of minerals (up to 1%) and titratable acidity from 19 to 25°T. A decrease in the values of total solids (19.2-16.6%), milk fat (8.0-6.2%), and protein content (5.8-4.9%) during the stages of lactation was established. The microbiological analysis showed that somatic cell count and total bacterial count during lactation progressively decreased, probably due to the account the hygienic conditions, nutrition, environment and health status of the animals. Moreover, changes in the composition of sheep milk were directly dependent on stable, stable/pasture and pasture feeding system during lactation period.


2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
K. Szajnar ◽  
M. Pawlos ◽  
A. Znamirowska

The global market for sheep’s milk and its products is increasing due to higher demand for cheese and traditional dairy products, and as a novelty, sheep’s milk is an ingredient in infant formulas and nutraceuticals. The aim of this study was to determine the properties of fermented sheep’s milk, which combines probiotic benefits with increased dietary fiber content. The influence of the applied dose of chokeberry fiber on the growth of living cells of Lactobacillus acidophilus and Lactobacillus rhamnosus in fermented sheep’s milk was also evaluated. Sheep milk with the addition of 0% (control sample), 1.5%, and 3.0% chokeberry fiber was fermented by two different probiotic monocultures L. acidophilus and L. rhamnosus. In the fermented milk, pH value, syneresis (%), color, and texture were determined. Furthermore, the microbiological analysis and an organoleptic evaluation were performed. With the increasing dose of chokeberry fiber, the pH value decreased already before the fermentation process. After fermentation, the milk’s pH values with fiber were still lower than those in the control sample. Presumably, more acidic metabolites were produced by L. acidophilus, causing a more intense reduction of the pH value than L. rhamnosus both in control milk and in milk with the addition of fiber. The addition of chokeberry fiber affected the stimulation of the growth of both types of bacteria. In the milk sample without fiber addition, a more significant number of viable cells were counted for L. acidophilus by 0.5 log CFU g-1 more than the milk fermented with L. rhamnosus. Furthermore, in milk fermented by L. acidophilus with 1.5% chokeberry fiber (LA1), the number of viable bacterial cells was higher than that in milk fermented by L. rhamnosus with the same addition of fiber (LR1). However, in sample LA3, the number of viable bacterial cells was lower than that in sample LR3. Tested fermented sheep milk met the Recommendation of the International Dairy Federation’s requirements regarding the number of live bacterial cells for dairy probiotics. The addition of chokeberry fiber increased syneresis in each fermented milk group, regardless of the bacteria used for fermentation. Moreover, the use of fiber caused a significant reduction in brightness L ∗ , an increase of red color, and a decrease of yellow color. Milk fermented with L. acidophilus was characterized by a harder gel, compared to their analogues fermented with L. rhamnosus. The addition of fiber intensified the sour taste and the taste of the additive in both types of fermented sheep milk.


Author(s):  
Tuba Büyüksırıt Bedir ◽  
Hakan Kuleaşan

The use of dried cheese in food production and food preparation sectors is becoming increasingly widespread. Dried cheese is preferred because of their longer shelf life at room temperatures, smaller packaging sizes, practical use, and their ease of homogenization in food products. Various technologies are currently being used for the drying of cheese and efforts are still being made to develop new methods. Freeze drying is a highly preferred technique for the production of dried cheese due to high quality of the final product. Instant removal of water and low process temperatures ensure an effective prevention of chemical and microbial deterioration. In this study, White, Kashar and Tulum cheeses were dried by freeze-drying method. Physicochemical analyses were carried out on the first day in order to determine the initial quality of cheese samples. The moisture and salt content were highest in White cheese, titratable acidity and fat content were highest in Tulum Cheese. Freze dried cheese samples were stored in room conditions for 6 months and changes in microbial populations such as mesophilic aerobic bacteria, yeast and moulds, lactic acid bacteria, Staphylococci spp. and coliform group bacteria were determined. The results showed that Tulum Cheese contained highest microbial counts in general. The number of bacteria was 7.71±0.10 log cfu/g in Tulum Cheese, 5.70±0.07 log cfu/g in White cheese. Total counts of yeasts and molds were 7.37±0.06 log cfu/g for Tulum cheese and 4.92±0.07 log cfu/g for Kashar Cheese. Statistical analysis was performedby using Minitab Statistical Package Program to evaluate the results of microbiological analysis on dried cheese samples.


2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
S. H. B. Dilrukshi ◽  
H. P. S. Senarath

Fruits and vegetables are healthy food sources which contain nutrients and phytochemicals with health-promoting properties. The production of healthy and more convenient products can be contributed to increase the consumption of fruits and vegetables. In this study, a novel, instant food product which is more nutritive was developed using locally available fruits and green leafy vegetables and the physicochemical, microbial, and sensory qualities of the product were studied. The most acceptable formula for the fresh green smoothie was 28.6% green content with 71.4% fruit content. The developed green smoothie was freeze dried to obtain an instant green smoothie powder. The instant green smoothie powder was analyzed for proximate composition: protein ( 2.67 ± 0.00 ), fat ( 1.96 ± 0.001 ), moisture ( 4.82 ± 0.003 ), ash ( 1.22 ± 0.000 ), and fiber ( 28.57 ± 0.008 ). This instant powder contained 129.5 ppm vitamin C content and higher amount of minerals such as K ( 0.98 ± 0.12   mg / g ), Ca ( 1.74 ± 0.03   mg / g ), and Fe ( 0.04 ± 0.004   mg / g ). The powder properties revealed that the powder has very good flowability ( 6.665 ± 2.354 ) according to the Carr Index and it has low cohesiveness ( 1.0713 ± 0.0264 ) according to the Hausner ratio. The solubility ( 94.71 ± 2.4253 ) of the powder complied with the SLS (668: 1984). The microbiological analysis revealed that this powder only contains 1 log CFU/ml of total plate count. This instant powder can be introduced as a more convenient and healthy choice for the consumers which has acceptable sensory properties, better microbiological stability, and very good powder properties.


2014 ◽  
Vol 884-885 ◽  
pp. 490-493 ◽  
Author(s):  
P. Rattanaburee ◽  
T. Amnuaikit ◽  
N. Radenahmad ◽  
J. Puripattanavong

Young coconut juice (YCJ) from aromatic green dwarf variety fruit of Cocos nucifera L. (Family Arecaceae) is high valuated because of its nutritional and pharmacological properties (wound healing, anti-Alzheimer, anti aging). Due to the storage and handling problems, YCJ has been prepared in dry form by freeze-drying process in this study. The % yield of freeze-dried YCJ powder was 6.62 ± 0.28 %. Both of them (fresh and freeze-dried YCJ) were further studied on phytochemical, physical, chemical and microbiological properties. The results showed that β-sitosterol was isolated and identified by means of spectral data and their physical properties. The HPLC-parameters (linearity, repeatability, accuracy and specificity) were evaluated. The concentrations of β-sitosterol in HPLC analysis of both fresh and freeze-dried YCJ were 89.67 ± 0.01 and 31.84 ± 1.56 μg/ml, respectively. Furthermore, there was no significant (p>0.05) difference in physicochemical analysis in between both products. Therefore, the microbiological analysis of these products was within standard range of Thai community products standard (TCPS). In conclusion, Freeze-dried YCJ was observed to be the suitable technique to obtain the dry extract of young coconut juice.


Author(s):  
Robert Glaeser ◽  
Thomas Bauer ◽  
David Grano

In transmission electron microscopy, the 3-dimensional structure of an object is usually obtained in one of two ways. For objects which can be included in one specimen, as for example with elements included in freeze- dried whole mounts and examined with a high voltage microscope, stereo pairs can be obtained which exhibit the 3-D structure of the element. For objects which can not be included in one specimen, the 3-D shape is obtained by reconstruction from serial sections. However, without stereo imagery, only detail which remains constant within the thickness of the section can be used in the reconstruction; consequently, the choice is between a low resolution reconstruction using a few thick sections and a better resolution reconstruction using many thin sections, generally a tedious chore. This paper describes an approach to 3-D reconstruction which uses stereo images of serial thick sections to reconstruct an object including detail which changes within the depth of an individual thick section.


Author(s):  
Jane A. Westfall ◽  
S. Yamataka ◽  
Paul D. Enos

Scanning electron microscopy (SEM) provides three dimensional details of external surface structures and supplements ultrastructural information provided by transmission electron microscopy (TEM). Animals composed of watery jellylike tissues such as hydras and other coelenterates have not been considered suitable for SEM studies because of the difficulty in preserving such organisms in a normal state. This study demonstrates 1) the successful use of SEM on such tissue, and 2) the unique arrangement of batteries of nematocysts within large epitheliomuscular cells on tentacles of Hydra littoralis.Whole specimens of Hydra were prepared for SEM (Figs. 1 and 2) by the fix, freeze-dry, coat technique of Small and Màrszalek. The specimens were fixed in osmium tetroxide and mercuric chloride, freeze-dried in vacuo on a prechilled 1 Kg brass block, and coated with gold-palladium. Tissues for TEM (Figs. 3 and 4) were fixed in glutaraldehyde followed by osmium tetroxide. Scanning micrographs were taken on a Cambridge Stereoscan Mark II A microscope at 10 KV and transmission micrographs were taken on an RCA EMU 3G microscope (Fig. 3) or on a Hitachi HU 11B microscope (Fig. 4).


Author(s):  
S.B. Andrews ◽  
R.D. Leapman ◽  
P.E. Gallant ◽  
T.S. Reese

As part of a study on protein interactions involved in microtubule (MT)-based transport, we used the VG HB501 field-emission STEM to obtain low-dose dark-field mass maps of isolated, taxol-stabilized MTs and correlated these micrographs with detailed stereo images from replicas of the same MTs. This approach promises to be useful for determining how protein motors interact with MTs. MTs prepared from bovine and squid brain tubulin were purified and free from microtubule-associated proteins (MAPs). These MTs (0.1-1 mg/ml tubulin) were adsorbed to 3-nm evaporated carbon films supported over Formvar nets on 600-m copper grids. Following adsorption, the grids were washed twice in buffer and then in either distilled water or in isotonic or hypotonic ammonium acetate, blotted, and plunge-frozen in ethane/propane cryogen (ca. -185 C). After cryotransfer into the STEM, specimens were freeze-dried and recooled to ca.-160 C for low-dose (<3000 e/nm2) dark-field mapping. The molecular weights per unit length of MT were determined relative to tobacco mosaic virus standards from elastic scattering intensities. Parallel grids were freeze-dried and rotary shadowed with Pt/C at 14°.


Sign in / Sign up

Export Citation Format

Share Document