Comparison of Two Protein Extraction Methods for Tea Residues

2011 ◽  
Vol 138-139 ◽  
pp. 933-936 ◽  
Author(s):  
Xuan Chen ◽  
Hong Yu Luo ◽  
Jun Yu ◽  
Peng Xiang Yue ◽  
Lin Zhou ◽  
...  
Keyword(s):  
High Temperature ◽  
Protein Extraction ◽  
Ethanol Concentration ◽  
Extraction Methods ◽  
Extraction Yield ◽  
Factorial Experiments ◽  
Extraction Technology ◽  
Digestion Method ◽  
Solid Ratio ◽  
Lower Temperature

Alcohol-alkali method and base digestion method were investigated to extract proteins from tea residues, respectively. According to single factorial experiments, results showed that the optimal extraction technology of alcohol-alkali method were pH 12, temperature of 80 °C, ethanol concentration of 60%, liquid-solid ratio of 40, 60 min, and the protein extraction rate reached 15.0%. And the optimal extract conditions of base digestion were pH 12, temperature of 80 °C, liquid-solid ratio of 50, 80 min, which made the protein yield reached 31.5%. Furthermore, alcohol-alkali method was more beneficial to protein extraction from tea residues under lower temperature and weak alkali condition (40-60 °C, pH 8-10). While base digestion had higher extraction yield under high temperature and strong alkali condition (60-80 °C, pH 11-12).

scholarly journals Comparison of Conventional and Sustainable Lipid Extraction Methods for the Production of Oil and Protein Isolate from Edible Insect Meal

Foods ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. 572 ◽  
Author(s):  
Laroche ◽  
Perreault ◽  
Marciniak ◽  
Gravel ◽  
Chamberland ◽  
...  
Keyword(s):  
Supercritical Co2 ◽  
Protein Extraction ◽  
Lipid Extraction ◽  
Tenebrio Molitor ◽  
Extraction Methods ◽  
Extraction Yield ◽  
Acheta Domesticus ◽  
Human Consumption ◽  
Alternative Source ◽  
Edible Insect

Edible insects represent an interesting alternative source of protein for human consumption but the main hurdle facing the edible insect sector is low consumer acceptance. However, increased acceptance is anticipated when insects are incorporated as a processed ingredient, such as protein-rich powder, rather than presented whole. To produce edible insect fractions with high protein content, a defatting step is necessary. This study investigated the effects of six defatting methods (conventional solvents, three-phase partitioning, and supercritical CO2) on lipid extraction yield, fatty profiles, and protein extraction and purification of house cricket (Acheta domesticus) and mealworm (Tenebrio molitor) meals. Ethanol increased the lipid extraction yield (22.7%–28.8%), irrespective of the insect meal used or the extraction method applied. Supercritical CO2 gave similar lipid extraction yields as conventional methods for Tenebrio molitor (T. molitor) (22.1%) but was less efficient for Acheta domesticus (A. domesticus) (11.9%). The protein extraction yield ranged from 12.4% to 38.9% for A. domesticus, and from 11.9% to 39.3% for T. molitor, whereas purification rates ranged from 58.3% to 78.5% for A. domesticus and from 48.7% to 75.4% for T. molitor.

scholarly journals Industry-Friendly Hydroethanolic Extraction Protocols for Grateloupia turuturu UV-Shielding and Antioxidant Compounds

2020 ◽  
Vol 10 (15) ◽  
pp. 5304
Author(s):  
Rafael Félix ◽  
Ana M. Carmona ◽  
Carina Félix ◽  
Sara C. Novais ◽  
Marco F. L. Lemos
Keyword(s):  
Antioxidant Activity ◽  
Ethanol Concentration ◽  
Extraction Procedure ◽  
Extraction Methods ◽  
Antioxidant Compounds ◽  
Uv Absorbance ◽  
Grateloupia Turuturu ◽  
Solid Ratio ◽  
Uv Shielding ◽  
Cosmetic Industry

Grateloupia turuturu is an invasive macroalga on the Iberian coast, known to produce bioactive compounds with different cosmeceutical bioactivities, namely UV shielding and antioxidants. The goal of this study was to optimize the extraction procedure of main bioactivities of this species with cosmetic potential, using Response Surface Methodology. Two Box–Behnken designs were used to evaluate the effect of ethanol concentration (0–50%), liquid-solid ratio, time, pH, and temperature on yield, UV absorbance, and antioxidant activity. Both optimizations showed a similar trend: aqueous extracts have higher yields and extracts performed with ethanol as part of the solvent have higher activities concerning UV absorbance and antioxidant activity. For all the extracts an absorption peak between 320 and 340 nm was observed. This data now allows further studies by narrowing the extracts worthful of characterization. The development of industry-friendly extraction methods allows the valorization of this invasive species, contributing for the potential creation of natural and eco-friendly products by the cosmetic industry while contributing to the restoration of affected environments.

Optimisation of the Ethanol-Based Heat Reflux Extraction of Isoflavones from Pueraria lobata (Willd.) Ohwi Root

2014 ◽  
Vol 955-959 ◽  
pp. 180-186 ◽  
Author(s):  
Shu Ya Wang ◽  
Jun Gao ◽  
Yi Lu Chen ◽  
Jun Zhou ◽  
Xiao Yu Yong ◽  
...  
Keyword(s):  
Large Scale ◽  
Ethanol Concentration ◽  
Orthogonal Experiment ◽  
Extraction Yield ◽  
Pueraria Lobata ◽  
Scale Production ◽  
Extraction Temperature ◽  
Extraction Technology ◽  
Large Scale Production ◽  
Solid Liquid

Ethanol-based heat reflux extraction has been successfully applied in extracting isoflavone compounds from the dried root of Pueraria lobata (Willd.) Ohwi. The different extraction factors including ethanol concentration, extraction temperature, extraction time, ratio of solid/liquid and number of extraction cycles has been studied on the influence of the extraction yield of isoflavone compounds. And the orthogonal experiment has been used to optimize the extracting conditions. A variety of indicators were employed to evaluate the extraction efficiency, including the yield of puerarin, daidzein and total isoflavone. The optimal extraction technology was as follows: extraction temperature 90°C, ratio of solid/liquid 1:6, ethanol concentration 90% and extraction 2 h. Under the optimal conditions extraction rates of puerarin, daidzein and total isoflavone were 37.51±0.64 mg/g, 2.63±0.03 mg/g and 117.4±0.89 mg/g. The study of this article can provide theoretical basis for large-scale production of puerarin, daidzein and total isoflavone.

scholarly journals Optimisation of High Hydrostatic Pressure Assisted Extraction of Anthocyanins from Rabbiteye Blueberry Pomace

2017 ◽  
Vol 35 (No. 2) ◽  
pp. 180-187 ◽  
Author(s):  
Zhang Haining ◽  
Ma Yongkun
Keyword(s):  
Hydrostatic Pressure ◽  
High Hydrostatic Pressure ◽  
Ethanol Concentration ◽  
Extraction Yield ◽  
Operating Conditions ◽  
Anthocyanin Content ◽  
Vaccinium Ashei ◽  
Box Behnken Design ◽  
Solid Ratio ◽  
Assisted Extraction

The purpose of this study was to evaluate the influence of high hydrostatic pressure assisted extraction (HHPE) on the anthocyanins from blueberry (Vaccinium ashei) pomace. From the Plackett-Burman Experimental Design (PBD) only the liquid-solid ratio, ethanol concentration, and extraction pressure were found to significantly affect the extraction yield of anthocyanin content. Hence, the outcome of Box-Behnken Design suggested that the optimal operating conditions of the HHPE for the yield of anthocyanin content were liquid-solid ratio 41 ml/g, ethanol concentration 63%, and extraction pressure 443 MPa. At these conditions, 107.9 mg/100 g anthocyanins was obtained, which was more than by the control extraction (67.63 mg/100 g). 10 anthocyanins were identified by HPLC-ESI-MS, malvidin-3-galactoside and malvidin-3-glucoside were the major anthocyanins.

Optimization and Comparison of Three Methods on Anthocyanins Extraction from Blackcurrant (Ribes nigrum L.) Using RSM

2011 ◽  
Vol 361-363 ◽  
pp. 691-700 ◽  
Author(s):  
Yu Yang ◽  
Sheng Xin Zhao ◽  
Ya Qin Xu ◽  
Ze Yuan Yu
Keyword(s):  
Ethanol Concentration ◽  
Extraction Methods ◽  
Perfect Agreement ◽  
Solid Ratio ◽  
Assisted Extraction ◽  
Natural Colorants ◽  
Ultrasonic Assisted ◽  
Optimal Values ◽  
Commercial Applications ◽  
Solid Liquid

Response surface methodology (RSM) complemented with a central composite design (CCD) was employed to optimize and compare three different anthocyanins extraction methods (solid–liquid extraction(SLE), ultrasonic-assisted extraction (UAE) and microwave-assisted extraction (MAE)) from blackcurrant fruits. The aim was to obtain extracts with high anthocyanins content, which would be potentially interesting for commercial applications as natural colorants. Three major independent variables such as extraction time(min), ethanol concentration (%,v/v), solution to solid ratio (mL/g) were coded at five levels and their actual values were selected on the basis of preliminary experimental results. Results indicated that the yields of anthocyanins varied when extracted with different method, the order of anthocyanins yield from high to low was: MAE (95.77%), UAE (93.65%) and SLE (90.82%). Among three reaction parameters, ethanol concentration consistently tended to significantly affect the anthocyanins yield for above three models (p < 0.0001). MAE was the best of three methods in this study and the optimal values were as follows: extract time controlled at 6.3 min, ethanol concentration maintained at 72.8%, solution to solid ratio equaled to 8.1:1 and working power at 400W. The experimental anthocyanins value under above optimum conditions could reach to 95.77±1.05% that was in perfect agreement with the predicted model 95.52%.

Study on the Optimization of Extraction Technology of Flavonoids in Clerodendrum inerme(L.) Gaertn. Roots

2012 ◽  
Vol 560-561 ◽  
pp. 349-354
Author(s):  
Jing Xu ◽  
Chuan Bo Cao ◽  
Dong Hai Li ◽  
Yan Feng Liu ◽  
Zhen Yi Liang ◽  
...  
Keyword(s):  
Ethanol Concentration ◽  
Extraction Yield ◽  
Extraction Time ◽  
Optimum Operating ◽  
Extraction Temperature ◽  
Liquid Ratio ◽  
Extraction Technology ◽  
Mangrove Plant ◽  
Operation Conditions ◽  
Total Flavonoids Content

Abstract. Based on the results of single factor experiments on the extraction of total flavonoids content (TFC) in roots of semi-mangrove plant Clerodendrum inerme(L.) Gaertn., operation conditions were optimized with orthogonal methodology. Four factors were applied to evaluate the extraction yield, including the ethanol concentration, extraction time, temperature and solid to liquid ratio. According to the experimental results, the influence order of different factors on the yield of TFC from the roots: ethanol concentration > solid to liquid ratio > extraction temperature > extraction time. The optimum operating parameters were determined as following, ethanol concentration of 70.0 %, extraction time of 90.0 min, temperature of 60.0 °C and solid to liquid ratio of 1:30. Under the optimized conditions, the maximal yield of TFC was enhanced to 1.279%.

scholarly journals Mechanical Processing of Hermetia illucens Larvae and Bombyx mori Pupae Produces Oils with Antimicrobial Activity

Animals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 783
Author(s):  
Alessio Saviane ◽  
Luca Tassoni ◽  
Daniele Naviglio ◽  
Daniela Lupi ◽  
Sara Savoldelli ◽  
...  
Keyword(s):  
Protein Extraction ◽  
Oil Quality ◽  
Antimicrobial Properties ◽  
Extraction Process ◽  
Extraction Methods ◽  
Extraction Yield ◽  
Oil Composition ◽  
Alternative Techniques ◽  
And Staphylococcus Aureus ◽  
By Products

The aim of this work was to develop processing methods that safeguard the quality and antimicrobial properties of H. illucens and B. mori oils. We adopted a vegetable diet for both insects: leftover vegetables and fruit for H. illucens and mulberry leaves for B. mori. First, alternative techniques to obtain a good oil extraction yield from the dried biomass of H. illucens larvae were tested. Traditional pressing resulted to be the best system to maximize the oil yield and it was successfully applied to B. mori pupae. Oil quality resulted comparable to that obtained with other extraction methods described in the literature. In the case of B. mori pupae, different treatments and preservation periods were investigated to evaluate their influence on the oil composition and quality. Interestingly, agar diffusion assays demonstrated the sensitivity of Gram-positive Bacillus subtilis and Staphylococcus aureus to H. illucens and B. mori derived oils, whereas the growth of Gram-negative Pseudomonas aeruginosa and Escherichia coli was not affected. This study confirms that fat and other active compounds of the oil extracted by hot pressing could represent effective antimicrobials against bacteria, a relevant result if we consider that they are by-products of the protein extraction process in the feed industry.

scholarly journals Box-Behnken experimental design for extraction optimization of cytotoxic activity from Curcuma aeruginosa rhizome

2020 ◽  
Vol 11 (2) ◽  
pp. 1631-1637
Author(s):  
Rani Khoiriyah ◽  
Made Artika I ◽  
Waras Nurcholis
Keyword(s):  
Experimental Design ◽  
Medicinal Plants ◽  
Cytotoxic Activity ◽  
Ethanol Concentration ◽  
Extraction Yield ◽  
Extraction Time ◽  
Pharmacological Activities ◽  
Solid Ratio ◽  
Bioactive Content ◽  
The Common

Curcuma aeruginosa is the common name of temuhitam in Indonesia, and the rhizome of parts plant had several pharmacological activities. Generally, pharmacological activities were associated with bioactive content in the extract of medicinal plants. Several factors can influence the bioactive extraction from medicinal plants such as solvent types, extraction time, extraction technique, and liquid-to-solid ratio. In this research, the extraction factors for extraction yield and cytotoxic activity of C. aeruginosa rhizome were optimized using the Box-Behnken experimental design. Effect of ethanol concentration, the ratio of liquid to solid, and extraction time for the maceration process was studied. The cytotoxic activity was determined by the brine shrimp lethality test. The optimum value that maximizes the extraction yield was 70 % ethanol, 300:15 ml/g liquid to solid ratio, and 1-day extraction time. The optimum value that maximizes the cytotoxic activity was 70 % ethanol, 150:15 ml/g liquid to solid ratio, and 2-day extraction time. The predicted extraction yield and cytotoxic activity at these projected values are 14.78 % and 78.26 mg/l, respectively. In this model, Adeq Precision (10.35 and 4.16), R-Squared (0.86 and 0.79), and F-value (7.92 and 2.04) is rational to fit the model for extraction yield and cytotoxic activity from C. aeruginosa rhizome.

scholarly journals Effect of Time, Solvent-Solid Ratio, Ethanol Concentration and Temperature on Extraction Yield of Phenolic Compounds From Olive Leaves

2018 ◽  
Vol 8 (2) ◽  
pp. 2805-2808
Author(s):  
N. Elboughdiri
Keyword(s):  
Phenolic Compounds ◽  
Ethanol Concentration ◽  
Extraction Yield ◽  
Extraction Time ◽  
Total Phenolic ◽  
Optimal Conditions ◽  
Total Phenolic Compounds ◽  
Olive Leaves ◽  
Factors Affecting ◽  
Solid Ratio

This study aims to determine the factors affecting the process of extraction of phenolic compounds from olive leaves. Two methods of extraction were used in this work and different tests were implemented with the aim of optimizing the recovery of phenolic compounds from olive leaves. The factors considered were the extraction time, temperature, solvent-solid ratio and the ethanol concentration. Obtained results show that the recommended optimal conditions for the extraction of the total phenolic compounds from olive leaves were found at 40°C with a solvent/solid ratio of 30:1 and ethanol concentration of 80% (v/v) when the dried olive leaves are stored at -21°C at the beginning of the experiments.

scholarly journals Analysis of Sweetness Components by Harvest Times and Extract Methods in Stevia

2019 ◽  
Vol 7 (1) ◽  
pp. 55
Author(s):  
Sang Un Kim ◽  
In Seok Um ◽  
Il Rae Rho
Keyword(s):  
High Temperature ◽  
Stevia Rebaudiana ◽  
Extraction Methods ◽  
Extraction Yield ◽  
Hot Water ◽  
Total Phenolic ◽  
Vacuum Extraction ◽  
Rebaudioside A ◽  
Stevia Rebaudiana Bertoni ◽  
Pressure Extraction

The leaves of Stevia rebaudiana Bertoni have attracted much attention as a source of natural sweeteners. This study was conducted to investigate the natural sweeteners contents and analyze antioxidant materials and activity according to harvest times and extraction methods in stevia.Stevia leaves were extracted using 5 extraction methods to identify the extraction with high extraction efficiency. Extraction yield was high in the order of hot water extraction (HWE), reflux extraction (RE), high temperature and pressure extraction (HTPE), ultrasonic extraction (UE) and vacuum extraction (VE). The contents of rebaudioside A and stevioside in stevia leaves had the highest in HTPE. Also, the total phenolic and flavonoid contents had the highest in HTPE and VE. Using the HTPE method, the result analyzed that the contents of rebaudioside A and stevioside of stevia leaves harvested between April and October exhibited the tendency to increase gradually between July and October. HTPE is considered to be an appropriate method for extracting stevia leaves. Also, it was confirmed that the stevia leaves of July, September and October, except for the high temperature period of August, had superior in quality and quantity.

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